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Evaluation of the Availability of Bolting Angelica gigas Nakai (추대 참당귀의 이용가능성 평가)

  • Lee, Sang-Hoon;Lee, So-Hee;Hong, Chung-Oui;Hur, Mok;Han, Jong-Won;Lee, Woo-Moon;Lee, Yi;Koo, Sung Cheol
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.318-324
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    • 2019
  • Angelica gigas Nakai (AGN) is a perennial herb belonging to the family Apiaceae. Its root has been utilized as a traditional medicine in Korea. Also, it contains decursin (D) and decursinol angelate (DA) as major active components. This study was performed to compare the change in content of active components and antioxidant activity between bolting and non-bolting AGN. Bolting AGN (B1) and non-bolting AGN (NB1) were harvested in the end of August. Additional non-bolting AGN (NB2) was harvested in the end of October. The total weight of B1 was 728.7 g, which was 98.5% of NB2 weight. Next, the AGN root (B1, NB1 and NB2) was divided into main root and lateral root. And the AGN aerial part (B1, NB1) was divided into flower, leaf and stem. The two active components, D and DA, and antioxidant activity were analyzed. The D content of B1 was 0.35-1.33% according to the plant parts and the DA content was 0.29-1.07%. In addition, B1 flower and leaf showed higher antioxidant activity than NB2 roots. The results show that B1 contained 15-56% of total major components compared with NB2 main roots, suggesting that B1 could be used as a potential material.

Cloning and Sequencing of the ${\alpha}-1{\rightarrow}6$ Dextransurcrase Gene from Leuconostoc mensenteroides B-742CB

  • Kim, Ho-Sang;Kim, Do-Man;Ryu, Hwa-Ja;Robyt, John-F.
    • Journal of Microbiology and Biotechnology
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    • v.10 no.4
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    • pp.559-563
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    • 2000
  • A dextransucrase gene (dsrB742) that expresses a dextransucrase to synthesize mostly ${\alpha}-1{\rightarrow}6$ linked dextran with a low amount (3-5%) of ${\alpha}-1{\rightarrow}3$ branching was cloned and sequenced from Leuconostoc mesenteroides B-742CB. The 6.1-kb PstI fragments were ligated with pGEM-3Zf(-) and transformed into E. coli $DH5{\alpha}$. The recombinant clone (pDSRB742) synthesized dextran on an agar plate containing 2% (w/v) sucrose. The dextran synthesized was hydrolyzed with Penicillium endo-dextranase. The hydrolyzate was composed of glucose, isomaltose, isomaltotriose, and branced pentasaccharide. The nucleotide sequence of dsrB742 showed one open reading frame (ORF) composed of 4,524 bp encoding dextrasnsucrase. The deduced amino acid sequence revealed a calculated molecular mass of 168.6 kDa. It also showed an activity band of 184 kKa on a non-denaturing SDS-PAGE (10%). The amino acid sequence of DSRB742 exhibited a 50% similarity with DSRA from L. mesenteroides B-1299, a 70% similarity with DSRS from L. mesenteroides B-512 (F, FMCM) and a 45-56% similarity with Streptococcal GTFs.

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광진단을 이용한 전자 에너지 분포 함수 변화 감지 알고리즘 개발

  • Park, Seol-Hye;Kim, Gon-Ho
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.08a
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    • pp.131-131
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    • 2010
  • 원자의 여기 및 천이에 의한 플라즈마에서의 빛 방출은 일차적으로 여기를 위한 특정 문턱값 이상의 에너지 공급이 전제 된다. 진공 플라즈마에서 대부분의 에너지 전달 과정은 전자와의 물리적 충돌에 의해 일어나므로 충돌 여기의 결과 발생한 광신호 세기는 전자 에너지 분포에 대한 정보를 내포하고 있다. 전자는 입자들 간의 에너지 전달 매개가 되는 동시에 플라즈마 구성 입자 중 가장 가벼워 빠르게 주변 환경 변화에 응답하여 열평형을 이루므로 EEDF는 플라즈마의 미세한 변동까지도 보여줄 수 있는 인자가 된다. 플라즈마의 열평형 이동에 관한 정보를 광신호로부터 EEDF의 형태로 추출해내기 위해 BEB (Binary - Encounter - Bethe) 모델을 근거로 충돌 반응 단면적을 함수로 나타내어 신호를 분석하였다. EEDF의 꼴을 $f(E)=AEexp(-E^b)$의 임의의 형태로 두고 아르곤의 427nm, 763nm 두 빛의 세기 비를 BEB 모델을 적용하여 전개한 결과 b factor 의 값을 구할 수 있었다. b factor 가 1인 경우는 Maxwellian, 2인 경우는 압력이 높은 조건에서 잦은 충돌에 의한 에너지 손실 때문에 고에너지 전자군이 현격하게 감소된 Druyvesteyn 분포를 의미하므로 광신호에 모델을 적용하여 얻은 b factor의 변화는 EEDF의 형태 자체의 변화가 감지되었음을 보여준다. 실제로 13.56MHz - 1kW ICP 장치에서 아르곤 플라즈마를 발생시켰을 때, 압력이 낮아 Maxwellian 분포가 예상되는 10mTorr 조건에서는 b=1.13, Druyvesteyn 분포에 가까워지는 100mTorr 조건에서는 b=1.502 로 관측되었다.

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Physiological Responses of Chromium on Radish(Raphanus Sativus L.) and Chinese Cabbages(Brassica campestris L.) in Hydrophonic Culture (수경재배시 무와 배추에 대한 크롬의 생리적 반응)

  • Han, Kang-Wan;Cho, Jae-Young;Choi, Jin-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.1
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    • pp.56-60
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    • 1998
  • Radish and Chinese cabbage were cultivated under hydrophonic culture to investigate the effect of chromium on germination, cell elongation, ${\alpha}$-amlyase activity, contents of chlorophyll and protein. With increasing concentration of cromium, germination, cell elongation, ${\alpha}$-amlyase activity were decreased in both radish and Chinese cabbage, the rate was higher in radish than in Chinese cabbage. Contents of chlorophyll a and b were also decreased and chlorophyll a was higher than chlorophyll b. As the concentration of chromium was increased inhibited in the order of protein> ${\alpha}$-amylase activity>chlorophyll a>chlorophyll b.

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CMOS true-time delay IC for wideband phased-array antenna

  • Kim, Jinhyun;Park, Jeongsoo;Kim, Jeong-Geun
    • ETRI Journal
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    • v.40 no.6
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    • pp.693-698
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    • 2018
  • This paper presents a true-time delay (TTD) using a commercial $0.13-{\mu}m$ CMOS process for wideband phased-array antennas without the beam squint. The proposed TTD consists of four wideband distributed gain amplifiers (WDGAs), a 7-bit TTD circuit, and a 6-bit digital step attenuator (DSA) circuit. The T-type attenuator with a low-pass filter and the WDGAs are implemented for a low insertion loss error between the reference and time-delay states, and has a flat gain performance. The overall gain and return losses are >7 dB and >10 dB, respectively, at 2 GHz-18 GHz. The maximum time delay of 198 ps with a 1.56-ps step and the maximum attenuation of 31.5 dB with a 0.5-dB step are achieved at 2 GHz-18 GHz. The RMS time-delay and amplitude errors are <3 ps and <1 dB, respectively, at 2 GHz-18 GHz. An output P1 dB of <-0.5 dBm is achieved at 2 GHz-18 GHz. The chip size is $3.3{\times}1.6mm^2$, including pads, and the DC power consumption is 370 mW for a 3.3-V supply voltage.

Inhibition of the Semaphorin 4D-Plexin-B1 axis prevents calcification in vascular smooth muscle cells

  • Hyun-Joo Park;Yeon Kim;Mi-Kyoung Kim;Hyung Joon Kim;Soo-Kyung Bae;Moon-Kyoung Bae
    • BMB Reports
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    • v.56 no.3
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    • pp.160-165
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    • 2023
  • Vascular calcification is common in cardiovascular diseases including atherosclerosis, and is associated with an increased risk of pathological events and mortality. Some semaphorin family members play an important role in atherosclerosis. In the present study, we show that Semaphorin 4D/Sema4D and its Plexin-B1 receptor were significantly upregulated in calcified aorta of a rat chronic kidney disease model. Significantly higher Sema4D and Plexin-B1 expression was also observed during inorganic phosphate-induced calcification of vascular smooth muscle cells. Knockdown of Sema4D or Plexin-B1 genes attenuated both the phosphate-induced osteogenic phenotype of vascular smooth muscle cells, through regulation of SMAD1/5 signaling, as well as apoptosis of vascular smooth muscle cells, through modulation of the Gas6/Axl/Akt survival pathway. Taken together, our results offer new insights on the role of Sema4D and Plexin-B1 as potential therapeutic targets against vascular calcification.

Factors influencing health-related quality of life in older adult women with sarcopenia: analysis of the Korean National Health and Nutrition Examination Survey 2019

  • Sol Hyun Lee;Ju-Hee Nho;Hye Young Kim;Eun Jee Lee
    • Women's Health Nursing
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    • v.29 no.4
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    • pp.328-336
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    • 2023
  • Purpose: This study aimed to identify factors influencing the health-related quality of life (HRQoL) of older adult women with sarcopenia. Methods: The study was secondary data analysis using data from the 2019 Korea National Health and Nutrition Examination Survey. The final sample consisted of 142 women aged 60 years and older with sarcopenia and were selected from 8,110 women. The participants was analyzed using complex descriptive statistics, independent t-test, and regression. Results: In terms of HRQoL, three general characteristics were found to be influential, with an explanatory power of 56.0%: difficulty climbing stairs, difficulty working, and perceived health status. Having no or mild difficulty when climbing stairs (B=.20, p=.001; B=.21, p<.001) and no or mild difficulty when working (B=.25, p=. 002; B=.208, p=.013) had a significant effect on HRQoL compared to severe difficulty. Having good or ordinary perceived health status had a significant effect on the HRQoL (B=.11, p<.001; B=.09, p<.001). Conclusion: Based on study findings that the HRQoL of older adult women with sarcopenia were influenced by difficulty climbing stairs and working, as well as good perceived health status, healthcare providers should assist elderly women to maintain physical activities in their daily lives.

Quality Characteristic of Glasswort (Salicornia herbacea L.) Fermented by Bacillus subtilis (Bacillus subtilis를 이용하여 발효시킨 퉁퉁마디(Salicornia herbacea L.)의 품질 특성)

  • Park, In-Bae;Park, Jeong-Wook;Lee, Young-Jae;Shin, Gung-Won;Kim, Hae-Seop;Jo, Yeong-Cheol
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.7
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    • pp.902-908
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    • 2009
  • In this study, we investigated the quality of glasswort (Salicornia herbacea L.) fermented by Bacillus subtilis at $37^{\circ}C$ for 48 hours. We determined the changes in temperature, the contents of moisture, crude protein, crude fat, crude ash, carbohydrate, solid content, pH, Brix, salinity, free amino acid, Hunter's color value, electron donating ability (EDA) activities and angiotensin converting enzyme (ACE) inhibition. The contents of crude protein, crude fat, crude ash and carbohydrate after fermentation to mixing ratio of glasswort were $10.45{\sim}30.18%$, $11.69{\sim}19.26%$, $17.65{\sim}25.56%$ and $21.01{\sim}59.10%$ (dry basis), respectively. The solid content, pH, sugar and salinity of fermented glasswort were $39.56{\sim}52.25%$, $6.01{\sim}6.71$, $1.2{\sim}1.7$ Brix and $0.3{\sim}0.6%$, respectively. Total amino acid level of the fermented glasswort was $447.57{\sim}668.89\;mg$/100 g, and was the highest in glasswort mixed with rice bran at a ratio of 1 (w) : 1 (w). Moreover, aspartic acid, asparagine, glutamic acid, alanine, valine, $\beta$-aminoisobutyric acid, lysine and arginine were the main free amino acids. EDA activities and ACE inhibition after fermentation were $46.66{\pm}1.21{\sim}50.87{\pm}1.84%$ and $96.77{\pm}1.23{\sim}97.56{\pm}1.23%$, respectively.

Variation in Agronomic Traits and Fatty Acid Compositions of the Seed Oil in Germplasm Collection of Brassica spp.

  • Ko, Ho-Cheol;Sung, Jung-Sook;Hur, On-Sook;Baek, Hyung-Jin;Lee, Myung-Chul;Luitel, Binod Prasad;Ryu, Kyoung-Yul;Rhee, Ju-Hee
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.590-600
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    • 2017
  • A total of 447 accessions consisting of seven Brassica spp.; Brassica carinata (34), B. juncea (199), B. rapa subsp. dichotoma (18), B. rapa. subsp. oleifera (14), B. rapa subsp. rapa (36), B. rapa subsp. trilocularis (56) and B. alba subsp. alba (90) were studied for their morphological characters and fatty acid compositions. There was a wide variation for morphological traits, oil content and fatty acid composition among Brassica species. Seed number/silique and yield/plant were varied from 4.2 (B. alba) to 25.1 (B. rapa subsp. trilocularis) and from 170.7 g (B. rapa subsp. oleifera) to 351.9 g (B. juncea L. Czern.), respectively. Among Brassica species, B. rapa subsp. trilocularis exhibited the highest oil (29.2%), stearic (20.4%) and erucic acid (45.3%) content. B. carinata had the highest content of palmitic (5.2%), oleic (21.2%) and linolenic acid (11.1%). B. rapa subsp. dichotoma and B. rapa subsp. oleifera exhibited the highest content of linoleic (8.1%) and behenic (26.9%) acid, respectively. B. rapa subsp. trilocularis exhibited the highest (45.3%) erucic acid content and significant positive relationship was observed between oleic acid and linoleic acid. This variation of agronomic and fatty acid compositions in Brassica species can be utilized to develop new varieties.

Comparison of Postoperative Stability between Distraction Osteogenesis and Bilateral Sagittal Split Ramus Osteotomy in Mandibular Retrognathism (하악후퇴증 환자에서 골신장술과 하악지 시상분할 골절단술의 술 후 안정성에 관한 비교 연구)

  • You, Myung-Su;Lee, Jee-Ho;Kim, Myung-Jin
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.34 no.2
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    • pp.100-105
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    • 2012
  • Purpose: In general, the surgical treatment for mandibular retrognathism is represented by two methods, distraction osteogenesis (DO) and mandibular osteotomy surgery. The DO is mostly preferred when the degree of advancement of mandible is large. However, the postoperative stability of mandibular advancement using DO have not been actively investigated. Therefore, in the present study we have compared the postoperative stability between DO and bilateral sagittal split ramus osteotomy (BSSRO) in mandibular retrognathism. Methods: Seven patients who had been treated by DO and thirteen patients with BSSRO were included in this study. Serial lateral cephalograms were analyzed by manual tracing and the amount of the mandibular elongation was measured. To evaluate the postoperative stability, positional changes of the condylar position and B point were analyzed. Results: Mean amount of mandibular advancement was $6.51{\pm}3.57mm$ for BSSRO group and $12.43{\pm}4.35mm$ for DO group, respectively. There was no significant difference in age between the two groups (P>0.05). Mean follow up periods were 10.77 months for BSSRO group and 11.28 months for DO group, respectively. After mandibular advancement, mean positional changes in the condyle were $0.56{\pm}1.43mm$ horizontally and $0.72{\pm}1.61mm$ vertically for BSSRO group and $0.53{\pm}1.56mm$ horizontally and $0.56{\pm}1.75mm$ vertically for DO group, respectively. Mean change of distance from B point to Y-axis was $-1.76{\pm}0.83mm$ for BSSRO group and $-2.14{\pm}1.82mm$ for DO group, respectively. According to the condylar position and B point, there were no significant differences in postoperative stability between the two groups (P>0.05). Conclusion: There was no significant difference in postoperative stability between DO and BSSRO group according to condylar position and B point. Based on the results of the present study, it is hypothesized that DO would be a good treatment choice for severe mandibular retrognathism because DO could achieve more mandibular advancement and concurrent soft tissue elongation.