• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.29-40
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• 2019

In Korea kiwifruit growing area is limited to southern coastal region and Jeju island, partly due to the lack of information on their cold hardiness in winter. This study was carried out to investigate cold hardiness of Korean kiwifruit cultivars in a period of dormancy for using it as preliminary data to expand the cultivation area of kiwifruit in Korea. A total of five kiwifruit cultivars in two species and hybrid, Actinidia deliciosa ('Hayward' and 'Garmrok'), A. chinensis ('Goldone') and A. arguta hybrid ('Bangwoori' and 'Skinny Green') were subjected to five freezing treatments of -12℃, -15℃, -18℃, -21℃ and -24℃. Cell membrane damage in all cultivars initiated in -18℃/32h and cell membrane stability was lost in -24℃ in most cultivars, except for 'Skinny Green'. Cold hardiness was estimated by 50% lethal temperature (LT₅₀) which was determined by triphenyl tetrazolium chloride (TTC) reduction. In branches, LT₅₀ was -15℃ in 'Hayward' and 'Garmrok', -18℃ in 'Bangwoori' and -21℃ in 'Goldone.' The LT₅₀ of buds on 'Hayward' and 'Garmrok' was 56 and 42 hours in -15℃ and 4 and 11 hours in -18℃, respectively; however, LT₅₀ of buds on 'Goldone' was 51 hours in -18℃ and that on 'Bangwoori' was 3 hours in -24℃. Cold hardiness results imply that it may be difficult for cultivars in A. deliciosa such as 'Hayward' and 'Garmrok' to be grown in the north of southern coastal region in Korea; however, it can be possible for several cultivars in A. chinensis and A. arguta hybrid to be grown in the northern part of Korean kiwifruit belt if cold tolerance in the thaw is confirmed.

• Applied Biological Chemistry
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• v.13 no.3
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• pp.219-222
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• 1970

Riboflavin in the various materials which used for Takju brewing was determined and its quantitative changes in the Takju mashes which were mashed with polished rice and wheat flour respectively using Nuruk only for enzyme source, and with wheat flour and corn powder respectively using Nuruk and Koji, were checked at 24 hours intervals during the whole brewing period. The results obtained were as follows. a) The riboflavin contents in 100 grams of each materials were: Polished rice $46.9{\gamma}$ Wheat flour $67.3{\gamma}$ Corn powder $73.9{\gamma}$ Nuruk $169.0{\gamma}$ Koji of wheat flour $87.1{\gamma}$ Koji of corn powder $84.4{\gamma}$ b) About 30 to 40 percent of riboflavin in the polished rice, wheat flour and corn powder were losed by steaming at 10 pounds for 50 minutes. c) Riboflavin content in the plots used Nuruk only was decreased during 3 days after mashing and then increased markedly after 5 days and, more or less, decreased after 6 days. Quantitative changes of riboflavin in the plots used Nuruk and Koji showed a rugged curve without decreasing in early stages, and its content was also increased markedly 5 days after mashing and then decreased a little after 6 days. d) Approximately $82{\gamma}$ of riboflavin was determined in 100ml. of the ripened mash of the rice plot and $72{\gamma}$ in the wheat flour plot in the case of using Nuruk only. $88.6{\gamma}$ of riboflavin were determined in 100ml. of the ripened mash of the wheat flour plot and $83.6{\gamma}$ in the corn powder plot in the case of using Nuruk and Koji. e) It was assumed that $28.8{\gamma}$ to $41{\gamma}$ of riboflavin were contained in 100 ml. of finished Takju of the two plots used Nuruk only and 33.6 to 44.5${\gamma}$ in the both two plots used Nuruk and Koji. f) About $47.3{\gamma}$ of riboflavin were determined in 100 grams of Takju cake of the rice plot and $66.2{\gamma}$ in the wheat flour plot used Nuruk only, and $62.9{\gamma}$ in the corn powder plot and $64.5{\gamma}$ in the wheat flour plot used Nuruk and Koji.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.197-205
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• 2007

This study was conducted to compare the effect of feeding molting and fasting molting on the performance, egg quality, and visceral organs in laying hens for animal welfare. Eighty one 62-wk-old White Leghorn hens that egg production was over 80% and average weight was $1.6{\pm}0.3\;kg$ were used in this study. Treatments were control (non-molt treatment), feeding molt treatment, and fasting molt treatment. The three treatments were administered to three replicate group of nine hens wherein each group. All treatment groups were fed the basal diet (CP 15%, ME 2,700 kal/kg) for two weeks as the adaptation period. Induced molt diets contains low CP (6.7%) and low energy (2,200 kal/kg). Test periods were 14 days for feeding molting and 10 days for fasting molting. Egg production decreased to be 0% at 10 days of feeding molting treatment, but at 2 or 3 days of fasting molting treatment. Egg production restarted after 19 days ending molt at feeding molting treatment, while after 24 days at fasting molting treatment. On the egg quality was improved at molting treatments (p<0.05) except egg yolk. Egg shell tissue was crowded at molting treatment to compare to control. Liver weights, heart weight, and oviduct weight of laying hens decreased at molting treatments (p<0.05). Finally, feeding molting might could be replaced fasting molting on the welfare and further studies were needed about molting program.

• Korean Journal of Poultry Science
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• v.34 no.3
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• pp.231-236
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• 2007

A feeding trial was conducted to study the effects of dietary supplement of fermented clay mineral (FCM) on the growth performance and immune stimulation in broiler chickens. A total of 36,800 broilers, (of) Ross strain-208, were randomly allotted into 2 experimental (diet) groups. Control group was fed the basal diet (corn-soybean meal based), and treatment group was fed the natural clay mineral that was fermented with aboriginal microorganisms at $37^{\circ}C$ for 72 h. Feed and water were provided ad libitum throughout the experiment for 5 weeks of experimental feeding period. Daily weight gain of treatment group fed 0.3% fermented clay mineral was significantly increased in comparison with control group. Feed intake of treatment group was significantly increased 21.4% (p<0.05), and feed efficiency was also increased 2.7% compared to control group. Especially, growth rate and production index of treatment group were significantly higher as much as 4.4% and 4.8%, respectively, than those of control group (p<0.05). By the feeding of dietary 0.3 % FCM, the antibody productions against Newcastle Disease and Infectious Bursal Disease antigens were shown to increase 13.8 % and 15.7 %, respectively, more than control group chickens. From these results, it appears that the supply of fermented clay mineral at 0.3 % level in the broiler chicken diet could enhance the growth performance and immune stimulation of broiler chickens.

• Journal of Embryo Transfer
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• v.20 no.3
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• pp.303-309
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• 2005

This study was conducted to examine the effects of OA on metaphase of meiosis II and the mitochondrial activity of cytoplasm in bovine cumulus oocytes complexes(COCs) during in vitro maturation. Hanwoo COCs were collected from the slaughterhouse cow ovaries and matured in TCM199 supplemented with $0.1\%$ PVA, 0.2 uM, 2 uM, 20 uM OA for the maturation rate of OA concentration. For the maturation effects between OA and cycloheximide(CX), COCs were matured in TCM199 with 25 ug/mL CX, 25 ug/mL CX (6 hrs culture) plus 2 uM OA or 2 uM OA only at a atmosphere $5\%\;CO_2,\;95\%$ air $39^{\circ}C$ for 6, 12, 24 hrs. To evaluate the nuclear types of matured COCs, cumulus cells were removedby $0.5\%$ hyaluronidase sol. and oocytes were fixed in 1:3 acetic acid ethyl alcohol for 30 sec. and then stained with $0.1\%$ basic Fuchsin sol. For the detection of fluoriscent intensity (FI) of matures oocytes, cumulus cells were removed same as performed above and were stained with 20 nM mite tracker for 20 min. at $39^{\circ}C$. Mitochondrial activity of FI in matured oocytes was imaged by laser conforcal microscopy (Fluoview, Olympus, Japan) and were measured scanned face on 5 um from median to endpoint of oocytes. Statical analysis of nuclear types observed the three replicates was carried out with ANOVA and Fisher's protected least significant difference test using the STATVIEW program. FI of matures oocytes was compared the multiples of the least intensity among the measured oocytes. Maturing in TCM199 supplemented with $0.1\%$ PVA, 0.2 uM, 2 uM, 20 uM OA, metaphase B were showed 72.0, 50.0, 70.0, $68.8\%$, respectively and there were different significant(p<0.05). In the case of treatment with OA and CX, metaphase were $73.8\%,\;8.2\%,\;45.5\%,\;73.7\%$ in $0.1\%$ PVA-TCM199, 25 ug/mL CX, 25 ug/mL CX plus OA or 2uM OA only, respeclively. FI was revealed the increasing tendency during the process of maturation. Whereas FI in CX was decreased about 3 times compared to the other treatments of 6 hrs maturation. We conclude that OA regulates bovine COCs maturation and induces the mitochondrial activity during the process of maturation.

• Journal of Life Science
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• v.23 no.3
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• pp.389-398
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• 2013

Platycodin D is a major constituent of triterpene saponins, which is found in the root of Platycodon grandiflorum, Platycodi Radix, which is widely used in traditional Oriental medicine for the treatment of many chronic inflammatory diseases. Several pharmacological effects of this compound have been reported recently, such as anti-inflammation, immunogenicity, anti-adipogenesis, lowered cholesterol, and anti-cancer activity. However, the mechanism by which this action occurs is poorly understood. In this study, we found that platycodin D greatly increased the potential of the anti-proliferative effect in various cancer cell lines. Our data revealed that platycodin D treatment resulted in a time- and concentration-response growth inhibition of U937 cells by inducing apoptosis, as evidenced by the formation of apoptotic bodies, chromatin condensation, and the accumulation of cells in the sub-G1 phase. Apoptosis induction of U937 cells by platycodin D correlated with an increase in the Bax/Bcl-2 ratio and caused the down-regulation of IAP family members. In addition, platycodin D treatment resulted in proteolytic activation of caspase-3, the concomitant degradation of poly(ADP-ribose) polymerases, and the collapse of the mitochondria membrane potential (${\Delta}{\Psi}_m$). However, the cytotoxic effects induced by platycodin D treatment were significantly inhibited by z-DEVD-fmk, a caspase-3 inhibitor, which demonstrated the important role that caspase-3 played in the observed cytotoxic effect. These findings suggest that platycodin D may be a potential chemotherapeutic agent for use in the control of human leukemia U937 cells. These findings also provided important new insights into possible molecular mechanisms of the anti-cancer activity of platycodin D.

• Korean Journal of Weed Science
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• v.18 no.2
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• pp.146-153
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• 1998

This experiment was conducted to clarify the characteristics of germination and emergence of Panicum dichotomiflorum which is a noxious weed species in direct-seeded rice field. P. dichotomiflorum was planted with several treatments such as different depths of irrigation to verifiy the ecological habits of seedling emergence and growth. In order to know the germination characteristics, Panicum dichotomiflorum seeds, pretreated with low-temperature($4^{\circ}C$) stratification for breaking the dormancy, were germinated under different temperature regimes and water potentials. Germination rates of P. dichotomiflorum was increased from 0% of dormant seed to 1%, 35% and 44% by stratification for 21, 28 and 42 days, respectively. Two dominant weed species in directseeded rice fields, Echinochloa crus-galli and Panicum dichotomitlorum, showed different germination habit under different temperature regimes. Echinochloa crus-galli showed more higher germination rate than Panicum dichotomiflorum at relatively low temperature regime(20/$10^{\circ}C$). Both species germinated faster at 30/$20^{\circ}C$ than at 20/$10^{\circ}C$. When the water potential was lowered, germination of Panicum dichotomiflorum was reduced more drastically than Echinochloa crus-galli. The critical water potential for germination of P. dichotomiflorum was -0.7MPa but Echinochloa crus-galli was affected slightly by the same water potential. The results showed that Echinochloa crus-galli can germinate under more wide range of soil water potential than Panicum dichotomiflorum. Emergence of P. dichotomiflorum was highly affected by irrigation depth and the level of water table. When the depth of irrigation water was increased, emergence of P. dichotomiflorum was dragged and emergence rate showed significant difference under the irrigation depth deeper than 6cm. The maximum depth of irrigation water for survival of Panicum dichotomiflorum seedling was 9cm.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.659-666
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• 2009

Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

• Proceedings of the Korean Society of Surveying, Geodesy, Photogrammetry, and Cartography Conference
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• 2002.04a
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• pp.3-10
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• 2002

요즘 측량분야에서 Geomatics 또는 Geoinformatics라는 새로운 용어가 대두되고 있다. 해외의 유수 기관 및 대학교에서도 측량이라는 학과명 또는 기관명을 Geomatics라는 용어로 교체하고 있으며, 이는 측량분야의 다양화와 학제간 연구수행의 필요성을 반영하는 것이다. 최근 45로 일컬어지는 GPS, 원격탐사(RS), GIS, ITS의 경우 각기 독자적인 영역을 구축하기보다는 상호 보완적인 역할을 하며 통합되어 가는 추세이다. 1950년대에 기본적인 위성관측기술 및 계산 기술의 개발을 시작으로 1980년대에 이르러서는 위성 기술은 측지학 및 측량 분야에 적용되기 시작했다. 그 대표적인 것이 GPS(Global Positioning System)로 기존의 천체측정학 방식을 대체하는 유용한 위치결정 수단으로 사용되기 시작했다. DNSS로부터 시작된 GPS는 측지 측량분야, 지구물리학분야, 항법 및 교통, GIS Mapping, 기상 및 해양, 재난 및 레저, 인공위성 궤도결정 등 다양한 분야에 적용되었으며, 특히 2000년 5월 1일 SA가 해제되면서 그 활용도는 더욱 증가하고 있다. 위성원격탐사의 경우 초창기에 중ㆍ저해상도의 다중분광영상에서 시작하여 그 해상도가 꾸준히 향상되어 오늘날 0.61 미터급 고해상도 위성인 QuickBird 위성이 발사되어 운용 중에 있다. 위성영상의 공간해상도, 분광해상도, 방사해상도는 향후 계속 향상될 것이며 이에 따른 방대한 데이터의 처리 문제 및 하드웨어/소프트웨어 지원에 대한 연구가 수반되어야 할 것이다. GPS 및 원격탐사(RS)는 더 이상 독자적인 영역으로서가 아니라 Geomatics를 이루는 중요한 분야로서 타 시스템과의 보완적인 관계로서 통합되어 나갈 것이다. 이를 위해서는 공간정보에 대한 표준화 및 데이터 처리, 통합, 시스템 구축을 위한 기술적 연구가 계속 진행되어야 할 것이다.분 공부상면적보다 늘어났다. 2. 좌표의 이동량이 일률적이지 못하므로 기초점에 대한 문제라고 생각되며, 따라서 도해지적을 수치지적으로 전환함에 있어서 가장 우선되어야 할 사항이 기초점 정비라 하겠다.승이 뚜렷하였다. 따라서 비파 착즙액 첨가 기능성 yoghurt 제조시 비파 착즙액을 10% 첨가하여 혼합 starter로 Str. thermophilus와 Lac. acidophilus 혼합균주를 사용하는 것이 이들 유산균의 증식에 가장 적합한 것으로 나타났다.타났다..297, 0.293, 0.205에서 50일 경과 후 0.612, 0.472, 0.898로 비살균에서 높은 값을 보였다. 따라서 비살균의 경우 저장말기에 TBA값이 높아지는 경향을 보였다. 5. L값은 살균처리의 경우 저장 30일 이후 약간 어두워지는 경향을 보였고, 121$^{\circ}C$ 살균처리에서 높은 값을 보였다. 대체로 저장온도가 높고 저장기간이 길어질수록 약간 밝아지는 경향을 보였다. 적색도는 인삼 첨가구의 경우 상온 및 냉장저장에서 10$0^{\circ}C$ 살균이 121$^{\circ}C$ 처리구 보다 약간 높은 값을 보였다. 저장기간에 따른 적색도의 변화는 인삼과 송이 첨가구에서 비교적 안정적이었다. 황색도는 상온 및 냉장저장에서 저장기간에 따라 약간 감소하는 경향을 보인 후 상온저장 50일 째, 냉장 60일 째 가장 높게 나타났다. 121$^{\circ}C$ 살균처리구가 10$0^{\circ}C$ 처리구보다 약간 높은 경향을 보였다.^{\circ}C$$,에서는 20시간 가열시 0.706$\mu\textrm{g}$/kg으로 가장 높게 생성된 후 서서히 감소하였다. 그러나 산값과 공액이중산값은 계속 증가하는 양상을 나타냈다. 즉 B(a)

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.891-900
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• 2003

Cellular FOS(c-fos) protein is a transcription factor that forms heterodimers mostly with c-jun family and stimulates the transcription of genes containing AP-1 regulatory elements. This c-fos expression can control growth and differentiation of various precursor cells including myoblasts. The controls by c-fos gene have been identified for affecting skeletal muscle fiber traits which are the key determinants of meat quality in pigs. As a first step for identifying the relationship between c-fos gene and meat quality traits in cattle, we fully sequenced 1,443 bp of Hanwoo c-fos mRNA and analyzed expression patterns from various organs and muscle tissues. The sequence identities of Hanwoo c-fos with that of human, pig and mouse showed 89.8%, 93.3% and 87%, respectively. Analyses of the northern blot showed high c-fos expressions were obtained in spleen and rib muscle from 7 organs and 9 different parts of muscles investigated. These results presented here can be used as a valuable marker for meat quality related traits in cattle with further verification.