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Antimutagenic Effect and Cytotoxicity to Human Cancer Cell Lines of Colored Potato Extracts (유색감자 추출물의 항돌연변이 및 항암 활성)

  • Park, Young-Eun;Jeong, Jin-Cheol;Cho, Huyn-Mook;Hwang, Young-Sun;Lee, Hyeon-Jin;Choi, Su-San-Na;Lee, Su-Jin;Park, Eun-Sun;Ko, Eun-A;Kim, Nan-Sol;Lim, Jung-Dae;Choung, Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.1
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    • pp.75-84
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    • 2008
  • Colored potatoes are an excellent source of dietary polyphenols including anthocyanins. Generally, anthocyanins from fruits and vegetables exhibit anti-carcinogenesis and anti-cancer properties in vitro test. This experiment was conducted to know the effects of colored potato extracts contained anthocyanins on antimutagenic activity and anticancer activity to six human cancer cell lines containing LNCaP (androgen-dependent) prostate cancer cells. Extracts of three colored potatoes ('Hongyoung', 'Jayoung' and 'Jasim') and the white potato ('Superior') cultivars were used in this study. The extracts of three colored potatoes inhibited the mutagenicities induced by direct mutagen such as 4-nitro-quinoline-1-oxide (4-NQO) and another indirect mutagens of bezo(a)pyrene (BaP). Also, the extracts of 'Hoyoung' and 'Jayoung' showed higher antimutagenic activity than 'Jasim' and 'Superior' against to direct or indirect mutagen on both strains of TA98 and TA100. The activity of growth-inhibitory of extract of four potato cultivars were screened by SRB (sulphorhodamine B) method on diverse human cancer cells representing different types of cancers. Among the extract of four potato cultivars, the extract of 'Jasim' showed moderate inhibition on proliferation of LNCaP, ACHN and MOLT-4F cells and did not inhibit the proliferation of other cancer cells. On the other hand, extract of 'Superior' did not inhibit the proliferation of any tested cancer cell lines. However, the extracts of 'Hongyoung and Jayoung' inhibited the proliferation of cancer cells with $GI_{50}$ values ranging from 2.5 to $30\;{\mu}g/mL$. On the basis of the $GI_{50}$ values, it is clear that LNCaP cells were more sensitive to extracts of colored potato cultivars than other cancer cells. The extract of 'Jayoung' at $30\;{\mu}g/mL$ were more active and inhibited cell proliferation, and induced apoptosis in LNCaP cells. This result revealed that the extracts of colored potatoes are expected to be good candidate for development into source of antimutagenic and anticancer agent.

A Study on Luxury Prohibition of Korean Personal Ornaments (한국장신구의 사치 금제 고찰)

  • 추원교
    • Archives of design research
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    • v.2 no.1
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    • pp.43-62
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    • 1989
  • The luxury is the expression of human being's ornament instinct. In this study, in order to grasp the moulding system of the Korean's personal ornaments, the process of luxury prohibition was reviewed to search for to which direction the ornaments developed in the frame of the prohibited style connected closely to the character of the personal ornaments. The proiod was fixed from the old society to the late Chosun dynasty era. The beginning of the luxury in Korea seems to be the start of the luxury burial at the time of funeral in the age of Koguryo., In the era of Koguryo, 10th year of King Dongmyung (B. C. 28), in the era of Baekje, 27th year of King Koi(260), the prohibition of dress regulation and the style of dress were conducted. The prohibition of personal ornaments in Silla was started from King Bup:Heung, and in the 9th year of King Heung-Duk, the prohibition was conducted in order to correct the luxury of the nobles and set up the social discipline. In the 11 th of King Il-Sung-Ni-Sa-Kum, the use of gold, silver and jade was prohibited in the civilian circles. The prohibition of Silla was succeeded to Koryo era, and in the 7th year of King Kwangjong(956), the system of Baekgwan Gongbok(uniform for government officials) was set up, and the system of Sasek Gongbok(four color official uniform) was set up in the 11 th year of the same King, and the prohibition of the personal ornaments such as crown and band is considred to have been conducted. The prohibition of gold and silver was conducted in the first year of King Sungjong(982), and in the 4th year of King Chungryul(l260), the order of wearing the dress and hat in accordance with the Yusan dynasty and the Mongolian customs were widely circulated in the royal court and vivilian circles. The strong influence of Mongolia made the taste of the traditional personal ornaments laste. The personal ornaments were used for the nobles until the age of the Unified Sillar but even the common people could use them in case they were rich, and such a circumstances made the use of foreign goods inflated. The prohibition of Koryo era was aimed at the prohibition of the foreign goods of luxury, and the classification of the social status.In the age of Chosun Dynasty, the production of gold and silver was feeble indeed but the oute reason of the prohibition was to eradicate the luxurious tendency, elevate the custom of eradicate the luxurious tendency, elevate the custom of thrift, and moreover, the gold, silver and jade were no the products of Korea and the prohibition was conducted but the true reason was afor the tribute tt China and the classification of status. The prohibition of Chosun dynasty was conducted first in the June of the 3rd year of King Taejo The major contents of prohibition was no use of gold, silver and jade, coral, agate, amber, etc. of th, wives of the Dang-Sang-Kwan (Court Nobleman) or their sons and daughters, and the same pheno menon was common even at the time of marriage. The people engaged in the secret trade there of wert beheaded. The personal ornaments in the prohibition were the pendent trinket, Binyo (crossbar) ceremonial ornamental hat, ring, earring, ornamental knife, hat string, hat ornament, belt, etc. Thl luxurious marriage expenses out of the luxury was severe, and lose of the marriageable age because 0 non-preparing the marriage goods was the national evil. The prohibition oC luxury was hard to bt kept to the nobles or rich people, the same as old days and present days. The prohibition of th{ luxury and personal ornaments of Korea had nothing to do with the commons, and it was limited tc the nobles and rich people. The prohibition was aimed to cultivating the custom of frugality by eradicating the luxurious atmosphere, but it was chiefly due to the tribute to the China and tht discrimination of the. status. We can say that the recent personal ornament was the flower of handi craft industry bloomed in the prohibition and regulation.

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Effect of Irradiation and Packaging Methods on the Oxidation of Cholesterol in Raw and Cooked Chicken Leg Meat (방사선 조사 및 포장방법이 생계육 및 조리계육의 콜레스테롤 산화에 미치는 영향)

  • Lee, J.I.;Shin, T.S.;Jin, S.K.;Kim, I.S.;Kim, Y.H.;Joo, S.T.;Park, G.B.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.825-834
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    • 2003
  • Chicken thigh from a retail market were used as experimental samples. Some chicken samples of raw state were packaged with PVDC at an aerobic and vacuum condition. The other samples were cooked until core temperature arrived at 70$^{\circ}C$ and then packaged immediately in the same way of raw samples. After samples were irradiated by electron beam at 6 kGy, they were stored in a refrigerator. Identification and quantity of cholesterol oxides were made at 0 and 7 days of storage, respectively. During the early stage of storage, 7$\beta$-hydroxycholesterol, $\alpha$,$\beta$-epoxide, cholestanetriol and 7-ketocholesterol were produced from the raw meat samples, and the production of these chemicals were significantly higher(P〈0.05) from the samples with aerobic packaging than those with vacuum packaging. With storage time, 7$\alpha$-hydroxycholesterol, 6-ketocholesterol and some other chemicals, which were not found during the early stage of storage, were found. Also, the formation of these chemicals were significantly increased(P〈0.05) with storage time. Cholesterol and lipid oxidation products of cooked meat after irradiation and irradiated meat after cooking were significantly increased(P〈0.05) with storage time for all treatments, and vacuum packaging results in showed significantly lower value(P〈0.05) than aerobic packaging. Summarizing the aforementioned results, it was found that the formation of cholesterol and lipid oxides and lipid oxidation was more easily affected by packaging condition than irradiation.

Estimation of Genetic Parameters of Body Weight by Growth Periods for Hanwoo Using Animal Model (개체모형에 의한 한우의 성장단계별 체중의 유전모수 추정)

  • Choi, J.G.;Jeon, K.J.;Lee, C.W.;Na, G.J.;Lee, C.;Kim, J.B.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.667-678
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    • 2003
  • The objectives of this study were to investigate the genetic characteristics of body weight by growth periods for Hanwoo. A total of 1,736 records were used for body weight. The data for body weights were collected from 1990 to 2000 in Daekwanryong branch, National Livestock Research Institute(NLRI). Estimates of (co)variance components were obtained by derivative-free Restricted Maximum Likelihood (DF-REML). The results are summarized as follows; The means for the weights were 25.60, 79.31, 98.91, 145.40, 283.26, 392.32, 545.65kg at birth, 3, 4, 6, 12, 18, 24month postpartum, respectively. The effects of calving year-season were significant for the milk yield of cow. Heritability estimates of direct genetic effects for birth weight were 0.54(all), 0.52(female), 0.36(male) in modelⅠ, 0.45(all), 0.41(female), 0.24(male) in modelⅡ, and heritabilities estimates of direct genetic effects for 4 month(weaning) weight were 0.47(all), 0.33(female), 0.28(male) in modelⅠ, 0.38(all), 0.21(female), 0.21(male) in modelⅡ. Heritability estimates for male and female data differed from those for combined data. The estimates became smaller for the body weights at 12 month or later(0.13~0.05). The heritabilities of average daily gain were smaller than those for body weights, but showed that the similar pattern to body weights.

A Monitoring for the Management of Microbiological Hazard in Rice-cake by Climate Change (기후변화에 따른 떡류의 미생물학적 위해관리를 위한 권역별 모니터링)

  • Choi, Song-Yi;Jeong, Se-Hee;Jeong, Myung-Seop;Park, Ki-Hwan;Jeong, Young-Gil;Cho, Joon-Il;Lee, Soon-Ho;Hwang, In-Gyun;Bahk, Gyung-Jin;Oh, Deog-Hwan;Chun, Hyang-Sook;Ha, Sang-Do
    • Journal of Food Hygiene and Safety
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    • v.27 no.3
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    • pp.301-305
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    • 2012
  • This study was conducted to investigate the microbiological contamination levels in rice cakes and rice flour due to climate change in three areas classified to their temperature and precipitation. We investigated the contamination levels of total aerobic bacteria, coliforms, Escherichia coli, Bacillus cereus, Staphylococcus aureus, Clostridium perfringens of rice flour and 3 rice cakes such as Garaetteok, Sirutteok and Gyeongdan. Contamination levels of total aerobic bacteria in rice flour were 4.9 log CFU/g. In a total of 70 rice flour, yeasts & molds and coliforms were detected in 42 and 52 samples at the levels of 43 CFU/g and 1.29 log CFU/g, respectively. S. aureus were detected in only 1 rice flour (1.66 log CFU/g) out of 70. In an investigation of contamination levels in rice cakes, the population of total aerobic bacteria were highest in Gyeongdan (5.18 log CFU/g) and coliforms were highest in Gareattock (2.93 log CFU/g). There was no detection of E. coli and B. cereus except for only 1 Gareattock (1.20 log CFU/g). There were no differences of contamination levels among the three areas. If constant monitoring of rice cakes and rice flour is conducted on the basis of this study, it is expected to be able to analyze the change of contamination levels in rice cakes and rice flour due to climate change.

Occupational Demands and Educational Needs in Korean Librarianship (한국적 도서관학교육과정 연구)

  • Choi Sung Jin;Yoon Byong Tae;Koo Bon Young
    • Journal of the Korean Society for Library and Information Science
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    • v.12
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    • pp.269-327
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    • 1985
  • This study was undertaken to meet more fully the demands for improved training of library personnel, occasioned by the rapidly changing roles and functions of libraries as they try to adapt to the vast social, economic and technological changes currently in progress in the Korean society. The specific purpose of this research is to develop a standard curriculum at the batchelor's level that will properly equip the professional personnel in Korean libraries for the changes confronting them. This study started with the premise that to establish a sound base for curriculum development, it was necessary first to determine what concepts, knowledge, and techniques are required for professional library personnel to perform it at an optimal level of efficiency. Explicitly, it was felt that for the development of useful curricula and courses at the batchelor's level, a prime source of knowledge should be functional behaviours that are necessary in the job situation. To determine specifically what these terminal performance behaviours should be so that learning experience provided could be rooted in reality, the decision was reached to use a systems approach to curriculum development, which is an attempt to break the mold of traditional concepts and to approach interaction from an open, innovative, and product-oriented perspective. This study was designed to: (1) identify what knowledge and techniques are required for professional library personnel to perform the job activities in which they are actually engaged, (2) to evaluate the educational needs of the knowledge and techniques that the professional librarian respondents indicate, and (3) to categorise the knowledge and techniques into teaching subjects to present the teaching subjects by their educational importance. The main data-gathering instrument for the study, a questionnaire containing 254 items, was sent to a randomly selected sample of library school graduates working in libraries and related institutions in Korea. Eighty-three librarians completed and returned the questionnaire. After analysing the returned questionnaire, the following conclusions have been reached: (A) To develop a rational curriculum rooted in the real situation of the Korean libraries, compulsory subjects should be properly chosen from those which were ranked highest in importance by the respondents. Characters and educational policies of, and other teaching subjects offered by, the individual educational institution to which a given library school belongs should also be taken into account in determining compulsory subjects. (B) It is traditionally assumed that education in librarianship should be more concerned with theoretical foundations on which any solution can be developed than with professional needs with particulars and techniques as they are used in existing library environments. However, the respondents gave the former a surprisingly lower rating. The traditional assumption must be reviewed. (C) It is universally accepted in developing library school curricula that compulsory subjects are concerned with the area of knowledge students generally need to learn and optional subjects are concerned with the area to be needed to only those who need it. Now that there is no such clear demarcation line provided in librarianship, it may be a realistic approach to designate subjects in the area rated high by the respondents as compulsory and to designate those in the area rated low as optional. (D) Optional subjects that were ranked considerably higher in importance by the respondents should be given more credits than others, and those ranked lower might be given less credits or offered infrequently or combined. (E) A standard list of compulsory and optional subjects with weekly teaching hours for a Korean library school is presented in the fourth chapter of this report.

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The Effect of Evaluation for Female teachers' Role-Performance on Their Appearance - according to clothing attitude of students and their parents - (학생과 학부모의 의복태도가 여교사의 외모관리에 따른 역할수행능력평가에 미치는 효과)

  • Yoo, Kyung-Ok;Chung, Myung-Sun;Wee, Eun-Hah
    • Journal of Korean Home Economics Education Association
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    • v.19 no.3
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    • pp.133-147
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    • 2007
  • The purpose of this study was to identify the effect on the evaluation of a female teachers' role-performance based on appearance according to the clothing attitude of students and their parents. Based on the results of this study a female teachers' role-performance evaluation can be broken down into four ability areas: leaning guidance, living guidance, human relations, and learning management. Likewise the clothing attitudes of students and their parents can be divided into three groups, the clothing oriented group, the trend-individuality group, and the chastity oriented group. The trend-individuality group of students felt that female teachers' appearances have a significant effect on learning guidance and human relations ability while parents thought that there was little relationship or that it has a moderate effect on the role of learning guidance. Because the concerns of students and parents about female teachers' clothing has an effect on female teachers' role performance evaluation, when directing a student group with a high concern for clothing, female teachers need to be sensitive about their appearances and it's affect on learning guidance, human relations, and class management. Based on these results, students and parents felt that female teachers' appearances have an effect on their role performance according to their clothing attitudes.

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Study on the Indoor Environment and Status of Facilities and Equipments of Home Economics Practice Rooms of Middle Schools in Jeju Special Self-Governing Province (제주특별자치도 중학교 가정실의 실내환경 및 시설.설비 현황에 관한 연구)

  • Park, Min-Hye;Kim, Bong-Ae
    • Journal of Korean Home Economics Education Association
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    • v.19 no.3
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    • pp.61-76
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    • 2007
  • The purpose of the study is to identify and understand problems existing in the middle school home economics practice rooms in Jeju Special Self-Governing Province. The findings are based on the examination and the analysis of the indoor environment and the condition of the facilities and equipment. Study method employs on-site research and a survey. The on-site research was conducted about temperature, humidity, intensity of illumination, and status of teaching instrument in 10 out of 41 middle schools in Jeju Special Self-Governing Province from August 16 to September 30, 2006. Meanwhile, the survey was implemented by mail for 95 teachers in charge of manual training and home economics education in 41 middle schools in Jeju from November 1 to 23, 2005. 64 questionnaires out of total 95 were collected, including those collected during the period of on-site research. Finally, 61 questionnaires which were effective among the answered ones were used for analysis. Collected materials were analyzed with the SPSS Win.12.0 program for frequency, percentile analysis. In conclusion, the study determines that the condition of the home economics practice rooms of the middle school in JSSGP in terms of temperature, humidity, lighting and ventilation is very inadequate. The structure of the practice room represents an inefficient work flow pattern. Further, the facilities and equipment are in a very poor condition because the facilities are old and the retention rate of teaching tools is low. Therefore, to address these problems, the study suggests that improvements on the facilities and equipment should be made and teaching tools should be replenished in accordance with the industry standard.

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Fragipan Formation within Closed Depressions in Southern Wisconsin, United States (미국 위스콘신 남부지방의 소규모 저습지에 나타나는 이쇄반층(Fragipan)의 형성과정에 관한 연구)

  • Park S.J.;Almond P.;McSweeney K.;Lowery B.
    • Journal of the Korean Geographical Society
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    • v.41 no.2 s.113
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    • pp.150-167
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    • 2006
  • This study was conducted to determine the pedogenesis of dense subsurface horizons (denoted either Bx or Bd) observed within closed depressions and in toeslope positions at loess-covered glacial tillplains in southern Wisconsin. Some of these dense subsurface horizons, especially those occurring within depressions, show a close morphological resemblance to fragipans elsewhere, even though the existence of fragipans has not been previously reported in southern Wisconsin. The spatial occurrence of fragipans was first examined over the landscape to characterize general soil-landscape relationships. Detailed physico-chemical and micromorphological analyses were followed to investigate the development of fragipans within a closed depression along a catenary sequence. The formation of fragipans at the study site is a result of sequential processes of physical ripening and accumulation of colloidal materials. A very coarse prismatic structure with a closely packed soil matrix was formed via physical ripening processes of loess deposited in small glacial lakes and floodplains that existed soon after the retreat of the last glacier. The physically formed dense horizons became hardened by the accumulation of colloidal materials, notably amorphous Si. The accumulation intensity of amorphous Si varies with mass balance relationships, which are governed by topography and local drainage conditions. Well-developed Bx horizons evolve at closed depressions where net accumulation of amorphous Si occurs, but the collapsed layers remain as Bd horizons at other locations where soluble Si has continuously been removed downslope or downvalley. Hydromorphic processes caused by the presence of fragipans are degrading upper parts of the prisms, resulting in the formation of an eluvial fragic horizon (Ex).

Comparison of Chemical Characteristics of Korean Mountain Ginseng Different Parts According to Extract Conditions (장뇌삼의 부위별 추출조건에 따른 이화학적특성 비교)

  • Kim, Jun-Han;Lee, Gee-Dong;Lee, In-Seon;Kim, Jong-Kuk
    • Food Science and Preservation
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    • v.13 no.6
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    • pp.720-725
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    • 2006
  • This study was conducted to investigate extracting solution effect on the chemical compositions in different parts of Korean mountain Ginseng. Water, 80% EtOH and 80% MeOH are used as extraction solutions, and extracting conditions were 2 hr at $85^{\circ}C$ in water bath. The Brix(%) of the extract were ranged from $0.42{\sim}22.58%$, 80% EtOH extract for leaf is the highest level as 22.58%. The pH ranges of the extracts were $4.43{\sim}7.41$ and brown color of the extract was the highest with 1.803 in 80% EtOH extract for leaf, respectively. In case of hunter's color value of the extract, L value is the highest with 24.35 in 80% EtOH extract of seed, a and b value were the highest with 0.41 in 100% water extract of leaf and 3.69 in 80% MeOH extract of stem. Sucrose is the major free sugar of the extinct it highest content with 3673 mg% in 80% MeOH extract of mot and fructose is the highest with 1897 mg% in 80% MeOH extract of leaf, Major organic acids are identified as malic, tartaric and citric acid, and total organic acid content is the highest with 5,254 mg% in 80% MeOH extract of leaf and 1,527 mg% in 80% EtOH extract of leaf, The extracted major minerals ate P and K, P content highest with 15,563 ppm in 100% water extract of stem, K is 4,952 ppm in 80% MeOH extract of leaf, and Ca is the highest with 3,052 ppm in 1011% water extract of leaf. These results suggest that extracting solvent (80% MeOH) is concerned with the extract preparation of Korea Mountain Ginseng.