• 한국잡초학회지
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• 제11권2호
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• pp.111-116
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• 1991

본(本) 연구(硏究)에서는 간척지(干拓地)의 우점잡초(優占雜草)인 새섬매자기의 방제(防除)를 위한 기초자료(基礎資料)를 얻고자 새섬매자거의 번식(繁殖)에 관한 생태적(生態的) 특성(特性)을 조사(調査), 검토(檢討)하였다. l. 새섬매자기의 구경(球莖)은 토양심도(土壤深度) 0-5cm에 16%, 5-8cm에 66% 그리고 8-10cm에 18% 각각 분포(分布)되였으며 평균분포심도(平均分布深度)는 6.51cm였다. 2. 구경(球莖)당 근경(根莖) 및 신아(新芽)의 수(數)는 각각 1개(個)에서 4개(個)까지로 근경(根莖)은 평균(平均) 2.34개(個), 신아(新芽)는 평균(平均)2.85개(個)였으며 전체(全體) 눈 (근경(根莖)과 신아(新芽))의 수(數)는 3-7개로 평균(平均) 5.2개(個)였다 3. 엽서(葉序)는 1/3이였으며 총(總) 12엽중(葉中) 4엽(葉)까지는 포엽(苞葉)이었다. 4. 파종구경(播種球莖)으로부터 출아주(出芽株)가 100주(株)로 증식(增植)되기까지의 일수(日數)는 3월(月) 25일(日) 출아(出芽)를 시작한 구경(球莖)에서 58일(日), 5월(月)7일(日) 출아구경(出芽球莖)에서 52일(日), 그리고 6월(月) 6일(日) 출아구경(出芽球莖)에서 43일(日)이 각각 소요(所要)되었다. 5. 파종구경(播種球莖)으로부터 번식(繁殖)에 따른 출아(出芽)(신구경발달(新球莖發達) 차수(次數)가 증가(增加)할수록 다음 출아(出芽)까지의 일수(日數)는 단축(短縮)되었고 근경장(根莖長), 초장(草長) 및 줄기의 지름은 점차(漸次) 증가(增加)되었다. 6. 초장(草長)은 직파재배(直播栽培)에서 재배초기(栽培初期)부터 벼보다 빠르게 신장(伸長)되었으며 이앙재배(移秧栽培)에서는 이앙후(移秧後) 약 30일(日)부터 벼의 것을 능가하였다. 7. 새섬매자기의 번식속도(繁殖速度)는 이앙재배(移秧栽培)보다 직파재배(直播栽培)에서 빨랐다.

• 가정과삶의질연구
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• 제9권2호
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• pp.1-18
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• 1991

The purpose of this study is to find out influential variables related to Home Management Ability of urban home makers. This study focuses on the following aspects; 1) to find out which variables of sociodemographic variables (ie. home maker's age, level of education-husband, wife, job-husband, wife, income, duration of marriage), of psychological variables (ie. degree of resourcefulness recognition, degree of stress recognition, degree of life level recognition) have significant effects on home management ability. 2) to find out which variables of sociodemographic variables have significant effects on degree of resourcefulness recognition, of stress recognition, and of life level recognition. 3) to identify the influence of significant variables related to home management ability. Data was analyzed by frequency. percentage, mean , F-test, t-test, Duncan's multiple range test. regression analysis , path analysis pearson's r. x2-test. Major findings are as follows; 1) The level of education (husband , wife)and occupation of husband were variables to have influences on home management ability. 2) a. The level of education (husband, wife) and income were variable to have influences on degree of resourcefulness recognition. b. The employment of home makers. income, and the form of family were variables to have influences on degree of stress recognition. c. The level of education (husband, wife) occupation of husband , income , and duration of marriage were variables to have influences on degree of life level recognition. 3) There were significant relationships between home management ability and degree of resourcefulness recognition and of stress recognition (r=0.13, r=-0.12, p<.05). a. The higher degree of resourcefulness recognition, the higher home management ability (x2=11.17. df=4. p<.05) b. The higher degree of stress recognition, the lower home n=management ability (x2=14.64. df=4. p<.01) 4) The education level of homemakers (β =0.15) and income (β=0.12) were variables to have indirect influences on home management ability through the medium of the degree of resourcefulness recognition (β =0.13) 5) The employment of home makers (β=-0.17) was a variable to have indirect influence on home management ability through the medium of the degree of stress recognition(β=-0.12) 6) the education level of husband (β=0.16) and income (β=0.32) were variables to have direct influence on degree of life level recognition. 7) The degree of life level recognition (β=0.13) and education level of home makers (β=0.17) were variables to have indirect influences on home management ability through the medium of the degree of resourcefulness recognition (β=0.13) 8)The degree of life level recognition (β=-0.22) the employment of home makers(β=-0.17) and the from of family(β=-0.10) were variables to have indirect influences on home management ability through the medium of the degree of stress recognition.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.117.3-118
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• 2003

We tested for the presence of double-stranded RNA (dsRNA) mycovirus in 827 Fusarium graminearum isolated from diseased barley and maize. dsRNA mycoviruses with various sizes were isolated. Of them, it was previously reported that dsRNA from DK2l isolate had pronounced morphological changes, including reduction in mycelial growth, increased to red pigmentation, reduced virulence and sporulation. (Chu et al., Appl. Environ. Microbiol. 2002). For better understanding of this hypovirulence associated with DK2l dsRNA virus, we determined the complete nucleotide sequence of dsRNA genome and named Fusarium hypovirus DK2l strain (Fhv-DK2l ). Genomic RNA of Fhv-DK2l was determined to be 6625 nucleotides in length excluding the poly (A) tail and contained three putative open reading frame. RNA-dependent RNA polymerase (RdRp) and helicase domain were expected in ORF A, 54 to 4709 nucleotide position. ORE B, 4752 to 5216 nucleotide position, and ORF C, 5475 to 6578 nucleotide position, were predicted to encode 16.7kDa and 41.3kDa protein respectively each. We could not detect any conserved domains from these two proteins. Phylogenetic analysis showed Fhv-DK2l was related to Cryphonectria hypovirus 3. Ten additional isolates were found that were infected with dsRNA mycoviruses. These mycoviruses contain 2 to 4 different segments of dsRNAs with the size range of approximately 1.7 to 10-kbp in length. The presence of dsRNAs isolates did not affect colony morphology and were transmissible through conidia and ascospore with incidence of 30-100％. These results indicate that there is genomic diversity of dsRNA mycoviruses that infect F. graminearum isolates and that impact of virus infection on host's morphology and virulence is determined by the interaction between dsRNAs and the fungal host, not by the mere presence of the dsRNAs

• BMB Reports
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• 제34권3호
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• pp.206-211
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• 2001

Endostatin, a proteolytic fragment of collagen XVIII, is a potent inhibitor of angiogenesis and the growth of several primary tumors. However, the opinions on the activity of endostatin derivatives deleted N- or C- terminal are still controversial. In this regard, we produced mouse endostatin and its derivatives in the prokaryotic system, and studied their anti-tumor activity. The [$^3H$]-thymidine incorporation assay demonstrated that N-terminal deleted mouse endostatin, and a C- and N-terminal deleted mutant, effectively inhibited the proliferation of human umbilical vein endothelial cells (HUVECs). The biological activity of endostatin was also shown by its in vivo anti-angiogenic ability on the chorioallantoic membrane (CAM) of a chick embryo. Treatment of $200\;{\mu}g$ of mouse endostatin, or N-terminal deleted mouse endostatin, inhibited capillary formation of CAM 45 to 71%, which is comparative to a 80% effect of positive control, $1\;{\mu}g$ of retinoic acid. An in vivo mouse tumor growth assay showed that N-terminal deleted mouse endostatin, and the N-/C-terminal deleted mutant, significantly repressed the growth of B16F10 melanoma cells in mice as did the full-length mouse endostatin. According to these results, N-and N-/C-terminal deleted mouse endostatins are the potent inhibitors of tumor growth and angiogenesis.

• Asian-Australasian Journal of Animal Sciences
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• 제16권5호
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• pp.672-676
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• 2003

Effect of a surfactant Tween 80 on the bacterial growth in the rumen was examined on the in vitro pure cultures of Streptococcus bovis, Selenomonas ruminantium, Butyrivibrio fibrisolvens, Prevotella ruminicola, Megasphaera elsidenni, Fibrobacta succinogenes, Ruminanococcus albus and Ruminococcus flavefaciens. Dry matter degradability (DMD), concentrations and compositions of volatile fatty acids (VFA), and the most probable number (MPN) of cellulolytic bacteria and total number of bacteria in the presence of Tween 80 were also examined on the in vitro rumen mixed culture either with barley grain or orchardgrass hay. The growth of S. bovis, S. ruminantium, B. fibrisolvens, P. ruminicola, M. elsidenni and F. succinogenes were significantly higher (p<0.05) at over 0.05% concentrations of Tween 80 than those of the control cultures, while was not changed with R. albus and R. flavefaciens. With rumen mixed culture the DMD of barley grain and orchardgrass hay was significantly higher (p<0.05) at a 0.2% concentration of Tween 80 than the control, being reflected in the significantly higher (p<0.05) VFA production (mmol $g^{-1}$DDM) with orchardgrass hay. The higher (p<0.05) ratio of propionate to acetate at a 0.2% concentration of Tween 80 was also observed with orchardgrass hay, showing a similar trend with barley grain. No changes in the total bacterial number and MPN of cellulolytic bacteria were observed.

• Journal of Nutrition and Health
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• 제37권8호
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• pp.623-632
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• 2004

This study was performed to investigate effect of dried powder or ethanol extracts of onion flesh and peel intakes on lipid metabolism, antioxidative and antithrombogenic capacities in l6-month -old rats. Total of 40 Sprague-Dawley male rats of l6-month-old and weighing 816$\pm$6g were blocked into 5 groups according to body weight and raised for three months with control and experimental diets containing 5% (w/w) of dried powders of onion flesh or peel or ethanol extracts from equal amount of each dried powder. Contents of total flavonoids and total dietary fibers in peel powder were highest among onion preparations. Body weight gain and epididymal pad fat weight were lower in peel powder group than other groups. Plasma total lipid, triglyceride and total cholesterol concentrations of onion-containing groups were lower than control group. Above all, peel ethanol extract intake decreased them most remarkably. Plasma HDL-cholesterol concentrations in onion-containing groups were higher than control group, especially that of flesh powder group was the highest among groups. Liver total lipid, triglyceride and total cholesterol concentrations were not significantly different among all experimental groups. However, liver total lipid and triglyceride concentrations were tended to be lower in onion-containing groups than control group. Thiobarbituric acid reactive substances (TBARS) concentrations in LDL + VLDL fraction was not significantly affected by onion intakes, However peel powder group showed the lowest concentration, Plasma TX $B_2$ concentrations in onion flesh powder, peel powder and peel ethanol extract groups were lower than control group, while plasma 6-keto-PG $F_{1a}$ concentrations in these same groups were higher than control group. Clotting time was tended to be increased in peel ethanol extract group. In conclusion onion diets seemed to improve lipid metabolism and antithrombogenic capacity while effect on antioxidative was not significant.t.

• Journal of Crop Science and Biotechnology
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• 제11권4호
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• pp.243-248
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• 2008

The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. But the information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatus resistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed from the cross PI 171451 ${\times}$ Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering 1073.9 cM on 20 linkage groups (LGs). With 126 $F_5$ RILs, two independent QTLs for resistance to R. clavatus were mapped on LGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escape response to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance to R. clavatus detected in the RIL population were not significantly affected by epistatic interactions.

• 한국균학회지
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• 제44권4호
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• pp.252-258
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• 2016

본 연구는 제주도 사려니숲과 동백동산에서 편백나무(Chamaecyparis obtusa), 삼나무(Cryptomeria japonica), 비자나무(Torreya nucifera), 꽝꽝나무(Ilex crenata), 동백나무(Camellia japonica) 등 5종의 목본 식물의 잎에서 내생균을 분리하고, 형태 및 분자생물학적 특징 통해 동정하였다. 이를 위해 primer인 ITS1F와 ITS4를 이용하여 internal transcribed spacer (ITS) rDNA영역과 primer LR0R과 LR16을 이용하여 large subunit rDNA 영역을 그리고 primer Bt2a과 Bt2b를 이용하여 ${\beta}$-tubulin 영역의 염기서열을 동시에 분석하였다. 그 결과 Mycosphaerella aleuritidis, Neofusicoccum eucalyptorum, Neofusicoccum parvum, Phyllosticta citrichinensis, Phyllosticta cryptomeriae, Phomopsis cotoneastri, Sphaerulina rhododendricola, Guignardia mangiferae, Lophodermium jiangnanense, Lophodermium minus 등 10종의 미기록 내생균을 동정하였고, 이를 보고하고자 한다.

• 한국식품영양과학회지
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• 제44권11호
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• pp.1759-1762
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• 2015

시판 막걸리에서 분리한 균주(Lactobacillus paracasei ssp. paracasei M13-65-3)로 타락을 제조하였다. M13-65-3균주를 이용하여 제조한 타락 추출물에 의하여 T lymphocyte인 Jurkat 세포의 증식이 촉진되었고 피부세포의 멜라닌 생성이 억제되었다. 본 연구의 결과는 타락이 면역 증강 기능성과 피부색소 조절 기능성을 보유할 가능성을 제시하고 있다.

• 한국전통의학지
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• 제15권1호
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• pp.70-76
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• 2006

The aim of this study was to investigate the effect of ethanol extract of Fagopyrum escuentum(FE) on the melanogenesis. To determine whether ethanol extract of FE suppress melanin synthesis in cellular level, B16F10 melanoma cells were cultured in the presence of different concentrations of FE ethanol extract. In the present study, the author examined the effects of FE ethanol extract on cell proliferation, melanin contents, tyrosinase activity. Cell proliferation was slightly increased by treatment with ethanol extract of FE (25-200 ${\mu}$g/ml). The ethanol extract of FE effectively suppressed melanin contents at a dose of 100 ${\mu}$g/ml. It was observed that the color of cell pellets was totally whitened compared with the control. The ethanol extract of FE inhibited tyrosinase activity, regulate melanin biosynthesis as the key enzyme in melanogenesis. These results suggest that the ethanol extract of FE exerts its depigmenting effects through the suppression of tyrosinase activity. And it may be a potent depigmetation agent in hyperpigmentation condition.