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Effect of Vitamin $B_{12}$ Addition on the Hatching Rate and Production of Resting Eggs of Freshwater Rotifer, Brachionus calyciflorus Pallas (Vitamin $B_{12}$ 첨가가 담수산 rotifer, Brachionus calyciflorus Pallas의 내구란의 생산과 부화율에 미치는 영향)

  • KWON O Nam;JO Soo-Gun;PARK Heum Gi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.2
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    • pp.160-163
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    • 2001
  • This study was performed to investigate the effect of vitamin $B_{12}$ addition on the production and hatching rate of resting egg of freshwater rotifer, Brachionus calyciflorus. Different concentrations of vitamin $B_{12}$ was added into concentrated freshwater Chlorella before fed to rotifer and rotifer was cultured in 250 mL flask. The maximum density of rotifer was 138 inds./mL at 6 ppm and it increased with an increase of vitamin $B_{12}$ addition. Mixis rate and fertilization rate of rotifer were $63.0\%$ and $89.6\%$, respectively, at 6.0 ppm. The production of resting eggs per mL was 231 eggs/mL at 1.5 ppm, but there was no significant difference among vitamin $B_{12}$ addition levels. The highest production of resting eggs per 10,000 rotifers and Chlorella dry weight (g) were 11,289 eggs and 121,252 eggs, respectively, at the 6 ppm. The hatching rate of resting egg was not significantly different among vitamin $B_{12}$ addition levels, but significantly higher than the control (without addition of vitamin $B_{12}$ ). These results showed that vitamin $B_{12}$ addition could affect production and hatching rate of resting egg of freshwater rotifer, B. calyciflorus.

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Preparation of B4C-Al2O3 Composite Powder by Self-propagation High-temperature Synthesis(SHS) Process under High Pressure (고압 자전 고온반응 합성법에 의한 B4C-Al2O3복합분말 제조)

  • 임경란;강덕일;김창삼
    • Journal of the Korean Ceramic Society
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    • v.40 no.1
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    • pp.18-23
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    • 2003
  • Composite powder of$B_4C-A1_2O_3$was prepared from a mixed powder of$B_2O_3/A1/C$by SHS under argon pressure instead of using a chemical furnace. A mixture of$B_2O_3,$Al and C powder (equivalent amounts to the reaction,$2B_2O_3+4A1+C=B_4C+2A1_2O_3)$was ball milled for 2 h. The mixed powder was placed in a SHS reactor and filled with 10 atm of argon gas and ignited. The inner and outer products were the same by XRD analysis. It was consisted of a composite powder of$B_4C-A1_2O_3$without $AlB_{12}/C_2$which was always produced using a chemical furnace. The composite powder was about$60~100{mu}m$size which was composed of crystalline particles of about 0.3~l${mu}m$size. But when 15 atm of argon was employed, partial sintering took place to give rise hard composite powder of$15~25{mu}m$$B_4C$with $0.1~0.2{mu}m$$A1_2O_3.$

Interaction between a Blood Vessel-Inducing Protein Angiogenin and Its Binding Protein Actin

  • Chang, Soo-Ik;Paik, Seung-Bum;So, Seung-Ho;Ahn, Byung-Cheol
    • BMB Reports
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    • v.29 no.4
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    • pp.353-358
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    • 1996
  • Bovine angiogenin (bAng) is a potent blood vessel inducing protein purified from cow In ilk. fluorescence spectroscopy has been used to study the interaction of bAng with actin in 50 mM Tris-HCl pH 7.5, and 1 mM $CaCl_2$ at $25^{\circ}C$. Actin contains four tryptophans but bAng contains no tryptophans. A 50% decrease in intrinsic fluorescence accompanied formation of the bAng/actin complex. By contrast, the interaction of RNase A, a homologous protein to bAng, with actin results in about 10% quenching of the fluorescence. Fluorescence titration experiments were performed by adding increasing concentrations of bAng (0~1.0 ${\mu}M$) to a constant concentration of actin (0.1 ${\mu}M$), and the dissociation constant $K_d$ for the bAng/actin complex and the stoichiometry n were measured as $20{\pm}1$ nM and $1.0{\pm}0.1$ respectively. These results suggest that the interaction between bAng with actin is specific and that quenching of actin fluorescence has occurred in the bAng/actin complex. The bAng binding sites of actin are discussed in the results of this study, and we propose that Trp-80 in the small domain of bovine actin is responsible for the bAng/actin binding.

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TABLES OF D-CLASSES IN THE SEMIGROUP $B_n1$ OF THE BINARY RELATIONS ON A SET X WITH n-ELEMENTS

  • Kim, Jin-Bai
    • Bulletin of the Korean Mathematical Society
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    • v.20 no.1
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    • pp.9-13
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    • 1983
  • M$_{n}$(F) denotes the set of all n*n matrices over F={0, 1}. For a, b.mem.F, define a+b=max{a, b} and ab=min{a, b}. Under these operations a+b and ab, M$_{n}$(F) forms a multiplicative semigroup (see [1], [4]) and we call it the semigroup of the n*n boolean matrices over F={0, 1}. Since the semigroup M$_{n}$(F) is the matrix representation of the semigroup B$_{n}$ of the binary relations on the set X with n elements, we may identify M$_{n}$(F) with B$_{n}$ for finding all D-classes.l D-classes.

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A 2.5V 80dB 360MHz CMOS Variable Gain Amplifier (2.5V 80dB 360MHz CMOS 가변이득 증폭기)

  • 권덕기;박종태;유종근
    • Proceedings of the IEEK Conference
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    • 2003.07b
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    • pp.983-986
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    • 2003
  • This paper describes a 2.5V 80dB 360MHz CMOS VGA. A new variable degeneration resistor is proposed where the dc voltage drop over the degeneration resistor is minimized and employed in designing a low-voltage and high-speed CMOS VGA. HSPICE simulation results using a 0.25${\mu}{\textrm}{m}$ CMOS process parameters show that the designed VGA provides a 3dB bandwidth of 360MHz and a 80dB gain control range in 2dB step. Gain errors are less than 0.4dB at 200MHz and less than 1.4dB at 300MHz. The designed circuit consumes 10.8mA from a 2.5V supply and its die area is 1190${\mu}{\textrm}{m}$$\times$360${\mu}{\textrm}{m}$.

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THE EFFECT OF XYLITOL ON THE EXPRESSION OF GTF GENE (gtf 유전자 발현에 대한 xylitol의 영향)

  • Yeom, Chung-Hyun;Chung, Jin;Jeong, Tae-Sung;Kim, Shin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.2
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    • pp.304-313
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    • 2004
  • Xylitol, a five-carbon natural sugar alcohol, is widely used non-cariogenic sugar substitute. In present study, the effects of xylitol on the expression of mRNA for glucosyltransferase which synthesizes glucan from sucrose were detected by Fluorescent in situ hybridization (FISH) and flow cytometry. FITC fluorescences for mRNA of gtfB, gtfC and gtfD were decreased further with increasing concentration of xylitol from 1% to 10% when detected by FISH. Flow cytometric analysis also showed that the expression of gtfB, gtfC and gtfD was increased by the addition of sucrose and decreased by the addition of xylitol to BHI broth containing 1% sucrose. In conclusion, the expression of gtfB, gtfC and gtfD mRNA was decreased by the addition of xylitol.

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Characteristics of $1{\times}N$ MMI Optical Power Splitters Fabricated by $Ag^+-Na^+$ Ion-exchange ($Ag^+-Na^+$ 이온교환법으로 제작된 $1{\times}N$ MMI 광파워 분리기의 특성)

  • Jeon, Keum-Soo;Jang, Myung-Ho;Kang, Dong-Sung;Kim, Hee-Ju;Pan, Jae-Kyung
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.38 no.8
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    • pp.34-42
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    • 2001
  • The 1${\times}$2, 1${\times}$4 and 1${\times}$8 multimode interference(MMI) optical power splitters are fabricated by using $Ag^+-Na^+$ ion exchange on BK7 glass. Before fabricating the MMI optical power splitters, we find the refractive index of the channel waveguide and calculate the multimode section length and width. The multimode section lengths and widths are 887${\mu}m$, 1666${\mu}m$ and 1834${\mu}m$ and 40${\mu}m$, 80${\mu}m$ and 120${\mu}m$ for 1${\times}$2, 1${\times}$4 and 1${\times}$8 MMI optical power splitters respectively. The measured properties of the fabricated MMI optical power splitters show that the unbalance ratios of the 1${\times}$2, 1${\times}$4 and 1${\times}$8 MMI optical power splitters are 1.4[dB], 1.7[dB] and 2.0[dB] and the excess losses of those sre 0.96[dB], 2.26[dB] and 1.67[dB]. respectively.

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Size and Resting Egg Formation of Korean Rotifer, Brachionus plicatilis and B. calyciflorus (한국산 Rotifer, Brachinus plicatilis와 B. calyciflorus의 크기 및 내구란 형성)

  • Hur, Sung-Bum;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.9 no.3
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    • pp.187-194
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    • 1996
  • Sixteen strains of marine rotifer, Braohionus plicatilis were isolated from salt pond, estuary and lagoon. Among 16 strains, 2 strains were large (L)-type and the others were small (S) or ultra small (US)-type. Four strains of fresh water rotifer, B. calyciflorus were isolated from commercial fish ponds. The size of lorica and resting egg were measured. In B. plicatilis, the range of lorica length from S-type and S-type were $244.3{\~}255.3\;{\mu}m$ and $131.0{\~}165.8\;{\mu}m$, respectively. The major axis of resting egg in the marine rotifer were $93.7\~116.4\;{\mu}m$ for S-type and $142.4{\~}145.5\;{mu}m$ for L-type, respectively. In freshwater rotifer, B. calyciflorus, the size range of lorica and major axis of resting egg were $211.8\~229.9\;{\mu}m$ and $126.8\~140.2\;{mu}m$, respectively. The size of freshwater rotifer was larger than that of S-type marine rotifer, but smaller than that of L-type one. Growth and formation of resting egg of B. plicatilis were different among the strains. The maximum density of S-type and L-type rotifer was 753.3 inds./ml for H-S strain and 220 inds./ml for O-L strain, respectively. The largest production of resting egg of S-type and L-type rotifer were 86.7 inds./ml for YY-S strain and 45.8 inds./ml for O-L strain, respectively.

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The Effect of Vitamin {TEX}$B_{6}${/TEX} Deficiency on the Utilization and Recuperation of Stored Fuel in Highly Intense Exercised Rats

  • Cho, Youn-Ok
    • Preventive Nutrition and Food Science
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    • v.1 no.1
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    • pp.75-79
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    • 1996
  • The purpose of this study was to investigate the effect of vitamin {TEX}$B_{6}${/TEX} deficiency on the utilization and recuperation of stored fuel in exercising rats. Thirty six rats were fed either a vitamin {TEX}$B_{6}${/TEX} deficient diet(-{TEX}$B_{6}${/TEX}) or a control diet(+{TEX}$B_{6}${/TEX}) for 5 weeks, then subdivided into 3 group: non-exercise group(NE), exercise group(EX), exercise and recuperation group(EX). EX group were exercised in treadmill({TEX}$10^{o}${/TEX}, 0.5~0.8km/h)for 2 hours and ER group were recuperated three days with the respective diet after exercise. Glucose(GLU), glycogen(GLY), protein(PRO), trigyceride(TG) and free fatty acid(FFA) were compared in plasma(P), liver(L) and skeletal muscle(M) of rats. Compared to +{TEX}$B_{6}${/TEX} rats, in NE group, the level of L-GLY of -{TEX}$B_{6}${/TEX} rats was higher, M-TG, L-PRO of -{TEX}$B_{6}${/TEX} rats were lower and there were no differences in P-PRO, P-FFA, P-TG, M-GLY, M-PRO and L-TG. In EX group, the levels of P-FFA, L-PRO of -{TEX}$B_{6}${/TEX} rats were higher. P-TG, L-TG of -{TEX}$B_{6}${/TEX} rats were lower and there was no difference in L-GLY. In EG group, the levels of P-GLU, P-PRO, P-RG, L-PRO of -{TEX}$B_{6}${/TEX} rats were lower and there were no differences in L-GLY, L-TG, M-TG and M-GLY. these results suggest that a lowered intake of vitamin {TEX}$B_{6}${/TEX} may impair the recuperation of aminals after exercise related to exercise fuel stores although there is a compensation among stored fuel utilization during exercise.

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10MHz/77dB dynamic range CMOS linear-in-dB variable gain amplifiers (10MHz/77dB 다이내믹 영역을 가진 선형 가변 이득 증폭기)

  • Cha, Jin-Youp;Yeo, Hwan-Seok;Kim, Do-Hyung;Burm, Jin-Wook
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.8
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    • pp.16-21
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    • 2007
  • CMOS variable gain amplifier (VGA) IC designs for the structure monitoring systems of the telemetries were developed. A three stage cascaded VGA using a differential amplifier and a linear-in-dB controller is presented. A proposed VGA is a modified version of a conventional VGA such that the gain is controlled in a linear-in-dB fashion through the current ratio. The proposed VGA circuit introduced in this paper has a dynamic range of 77 dB with 1.5 dB gain steps. It also achieved a gain error of less than 1.5 dB over 77 dB gain range. The VGA can operate up to 10MHz dissipating 13.8 mW from a single 1.8 V supply. The core area of the VGA fabricated in a Magnachip $0.18{\mu}m$ standard CMOS process was about $430{\mu}m{\times}350{\mu}m$. According to measurement results, we can verify that the proposed method is reasonable with regard to the enhancement of dynamic range and the better linear-in-dB characteristics.