• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1475-1481
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• 2008

This study was to investigate the inhibitory effects of Mori Fructus on the generation of peroxynitrite ($ONOO^-$), nitric oxide (NO) and superoxide anion radical (${\cdot}O_2{^-}$) in the endothelial cells of rat vessels. The aim of this study was to investigate the $ONOO^-$, NO, ${\cdot}O_2{^-}$ scavenging and anti-inflammatory activitives of Mori Fructus. For this study, the fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed using anti-NF-${\kappa}B$ (p50, p65), anti-COX-2, anti-iNOS antibodies, respectively. Mori Fructus prevented lipopolysaccharide (LPS)-induced cell death in YPEN cells. Mori Fructus inhibited the generation of $ONOO^-$, NO and ${\cdot}O_2{^-}$ in the LPS-treated cells. Mori Fructus inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-${\kappa}B$ activation. These results suggest Mori Fructus is effective on inhibiting the generation of $ONOO^-$, NO and ${\cdot}O_2{^-}$, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.

• Composites Research
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• v.19 no.2
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• pp.28-34
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• 2006

[ $Cu-TiB_2$ ] metal matrix composites with various weight fractions of $TiB_2$ were fabricated by combination of manufacturing process, SPS (self-propagating high-temperature synthesis) and SPS (spark plasma sintering). The feasibility of $Cu-TiB_2$ composites for welding electrodes and sliding contact material was investigated through experiments on the tensile properties, hardness and wear resistance. To obtain desired properties of composites, composites are designed according to reinforcement's shape, size and volume fraction. Thus proper modeling is essential to predict the effective material properties. The elastic moduli of composites obtained by FEM and tensile test were compared with effective properties from the original Eshelby model, Eshelby model with Mori-Tanaka theory and rule-of-mixture. FEM result showed almost the same value as the experimental modulus and it was found that Eshelby model with Mori-Tanaka theory predicted effective modulus the best among the models.

• Journal of Sericultural and Entomological Science
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• v.28 no.1
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• pp.15-23
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• 1986

This study has been carried out to acquire some basic informations on the digestive fluid of Silkworm, Bombyx mori for developing artficial diets. Silkworms reared on mulberry leaves and artificial diet, were used in this experiments. The results obtained are as follows ; 1. The Red Fluorescent protein was precipitated in 50% acetone solution and did not dissolved in n-butanol solution, but dissolved in methanol solution. 2. Electrophoretic analysis results of mulberry leaves rearing B. mori and artificial diet rearing B. mori, which has been treated with 50% acetone solution were as follows. i) There was distinct difference at the position of high mobility ii) Red Fluorescence was observed only at the position of first band of mulberry leaves rearing B. mori. iii) No different was shown in the electrophoretic patterns of mulberry leaves rearing B. mori on 5th instar 1st-3rd day, but some difference on 5th instar 4th, 5th day. 3. The RFP is the basic protein which has PI 8-9 according to the isoelectric electrophhoresis. 4. The SDS-polyacrylamide gel electrophoresis analysis showed that the molecular weight of RFP was 27,000. 5. The Sephadex G-75 chromatographic analysis showed that there was three peaks between number 16 and 28 in the mulberry leaves rearing B. mori chromatogram, but two peaks between number 18 and 31 in the artifical diet rearing B. mori chromatogram.

• Korean journal of applied entomology
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• v.43 no.2
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• pp.143-153
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• 2004

Non-spinning syndrome of Bombyx mori has been serious issue in sericulture industry near Kyungbuk area. This study was focused on the analysis of the mechanism and on screening candidate chemicals inducing the anti-metamorphosis of the silkworms. Rearing temperatures or initial body weight of the final instar larvae did not affect a normal larval to pupal metamorphosis of B. mori. However, pyriproxyfen (a juvenile hormone (JH) agonist) induced follicle patency significantly even at its 10$\^$-8/ M concentration and inhibited metamorphosis of B. mori in both developmental time and dose dependent manners. Pyriproxyfen induced JH esterase (JHE) activity and downregulated expression of JH binding protein of 5. mori. These results suggests that pyriproxyfen induced JHE activity as a JH agonist and that the elevated JHE activity degraded endogenous JH and resulted in JHBP gene expression. Based on the fact that the JH agonist induced follicle patency and inhibited metamorphosis of B. mori, follicle patency bioassay suggested that three commercial pesticides including simazine, molinate or alachlor were proved to give potent JH agonistic effect on B. mori. Further direct exposure experiments to these candidates are required to determine the chemicals responsible for the non-spinning syndrome of 8. mori.

• International Journal of High-Rise Buildings
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• v.10 no.3
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• pp.243-250
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• 2021

Y

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.35-40
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• 2004

Bombyx mori densonucleosis virus type 1 (BmDNV1)- a non occluded virus causes flacherie disease in the susceptible stocks of the silkworm, Bombyx mori. However, some stocks are non-susceptible. Non-susceptibility to BmDNV1 in B. mori is a unique case where the virus infection is completely inhibited by a single gene of the host. A survey conducted by this institute in some parts of Karnataka state has revealed that, 43.05% of the total incidence of flacherie disease caused by non-occluded viruses, are due to the synergistic infection of B. mori densonucleosis and infectious flacherie virus. Earlier study indicated that rearing of BmDNV1 resistant silkworm stock is effective in protecting silkworm against BmIFV also. In the present study the response of 78 silkworm stocks which include 42 of non-diapausing and 36 of diapausing groups, to BmDNV1 is investigated. Newly ecdysed third instar larvae were inoculated per-os with 10% inoculum of BmDNV1 extracted from the mid-gut of infected silkworm. One non-diapausing and three diapausing silkworm stocks were found to be resistant to BmDNV1. Eleven silkworm stocks were found to possess moderate resistance whereas rest sixty three were found to be susceptible to BmDNV1. Genetic analysis has shown that the resistance to BmDNV1 is autosomally inherited and controlled by a major dominant or a major recessive gene in different silkworm stocks. These resistant stocks can be utilized as the resource material to develop BmDNV1 resistant commercial hybrids. The selection strategies, depending upon the mode of inheritance of resistance in the resource material chosen, are discussed.

• Journal of Life Science
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• v.8 no.3
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• pp.285-293
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• 1998

We have cloned an internal fragment of the putative transoisase gene of MLE in the silkworm, Bombyx mori, using PCR method with degenerative oligonucleotide primers designed to represent regions of amino acids encoding transposase. The resulting PCR clone, designed as BmoMAR, cords a partial ORF(152 a.a.) of MLE in which interrupted by five stop codons, and the sequence of its deduced amino acids showed 37% homology with Mos1, an active mariner, from Drosophila mauritiana. Furthermore, the BmoMAR exhibits nucleotide and amino acid homology with 59% and 37% from Apis mellifera and D. mauritiana 7.9 clone, respectively. This result strongly that a MLE is present in the genome of B. mori.

• International Journal of Industrial Entomology and Biomaterials
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• v.4 no.1
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• pp.31-36
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• 2002

Peptidohlycan recognition protein (PGRP) is one of the pattern recognition proteins in innate immunity of insect. We isolated differentially expressed two cDNAa, BTL-LPI and BTL-LP2, in the fat body of Bombyx mori larvae injected with bacteria by subtractive hybridization method. These two clones showed amino acid sequence divergence of 30.4%. In the comparison with other insect PGRP genes, BTL-LP2 showed 48.8% and 45.2% of sequence homology to the known PGRP genes of Bombyx mori and Tricoplusia ni, respectively, and BTL-LP2 was 31.8% and 30.9% , respectively. Phylogenetic analysis showed relatively close relationship of the BTL-LP2 to the known insect PGRP, unlike BTL-LPI, which was equidistant both to insect and mammals, suggesting a divergent relationships of the two newly cloned B. mori PGRP genes. Northern blot analyses confirmed an induction of the expression of BTL-LP2 by the bacterial infection in the Int body of B. mori, suggesting the involvement of the gene in the insect immunity.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.24 no.3
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• pp.45-50
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• 1998

13 develop an active agent for skin whitening, we examined the inhibitory effects of 285 plant extracts on tyrosinase activity and found some plant extracts have an ability to inhibit tyrosinase activity. Of these plant extracts, ramulus mori extracts showed the highest inhibiting activity on enzyme activity. From these results, we selected ramulus mori as a new active agent for skin whitening. $IC_{50}$/ of this extracts on tyrosinase activity is 12.48$\mu\textrm{g}$/m1. Also, ramulus mori extracts showed inhibitory activity on melanin synthesis in B-16 melanoma cells. We isolated active compound from ramulus mori extracts using various column chromatography and recrystalization and identified its chemical structure using Mass spectroscopy, IR and NMR analysis. So, it is identified as Mulberrin, a maior compound of ramutus mori and turned out that it has potent ability on tyrosinase inhibition. $IC_{50}$/ of this compound for tyrosinase activity is 0.5$\mu\textrm{g}$/ml. Also, this compound showed potent inhibitory activity on melanin synthesis in B-16 melanoma cells. This extracts was turned out to be safe in many safety tests.

• Journal of Sericultural and Entomological Science
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• v.37 no.1
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• pp.57-61
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• 1995

In order to isolate naturally occuring novel B. thuringensis strains, we identified the distribution of B. thuringiensis from soils of sericultural farms(spring and autumn season, respectively) of various regions in Korea. A total of 82 strains of B. thuringiensis producing spore and crystal were isolated. We examined oral toxicity against lepidopterous larvae, Bombyx mori, dipterous larvae, Culex pipiens and coleopterous, Sitophilus oryzae. The results showed that 82 B. thuringiensis isolates from sericultural farms were isolated and most isolates were toxic to lepidopterous larvae. Particularly, B. thuringiensis strains which is lepidopterous-active, lepidopterous-and dipterous-active were widely distributed. Also, non-toxic B. thuringiensis isolate was common.