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Isolation of Two cDNAs Encoding a Putative Peptidohlycan Recognition Protein Gene from the Domestic Silkworm, Bombyx mori  

Kim, Sang-Hyun (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration)
Lee, Heui-Sam (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration)
Kim, Jin-Won (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration)
Lee, Young-Sin (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration)
Kim, Iksoo (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology, Rural Development Administration)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.4, no.1, 2002 , pp. 31-36 More about this Journal
Abstract
Peptidohlycan recognition protein (PGRP) is one of the pattern recognition proteins in innate immunity of insect. We isolated differentially expressed two cDNAa, BTL-LPI and BTL-LP2, in the fat body of Bombyx mori larvae injected with bacteria by subtractive hybridization method. These two clones showed amino acid sequence divergence of 30.4%. In the comparison with other insect PGRP genes, BTL-LP2 showed 48.8% and 45.2% of sequence homology to the known PGRP genes of Bombyx mori and Tricoplusia ni, respectively, and BTL-LP2 was 31.8% and 30.9% , respectively. Phylogenetic analysis showed relatively close relationship of the BTL-LP2 to the known insect PGRP, unlike BTL-LPI, which was equidistant both to insect and mammals, suggesting a divergent relationships of the two newly cloned B. mori PGRP genes. Northern blot analyses confirmed an induction of the expression of BTL-LP2 by the bacterial infection in the Int body of B. mori, suggesting the involvement of the gene in the insect immunity.
Keywords
Peptidoglycan recognition protein gene; PGRP; Silkworm; Bombyx mori; Insect immunity;
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