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Inhibitory Effects of Mori Fructus on the Peroxynitrite and Proinflammatory Proteins  

Woo, Sung-Ho (Department of Internal Medicine, College of Korean Medicine, Dongguk University)
Jeong, Ji-Cheon (Department of Internal Medicine, College of Korean Medicine, Dongguk University)
Shin, Hyeon-Cheol (Department of Internal Medicine, College of Korean Medicine, Daegu Hanny University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.6, 2008 , pp. 1475-1481 More about this Journal
Abstract
This study was to investigate the inhibitory effects of Mori Fructus on the generation of peroxynitrite ($ONOO^-$), nitric oxide (NO) and superoxide anion radical (${\cdot}O_2{^-}$) in the endothelial cells of rat vessels. The aim of this study was to investigate the $ONOO^-$, NO, ${\cdot}O_2{^-}$ scavenging and anti-inflammatory activitives of Mori Fructus. For this study, the fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed using anti-NF-${\kappa}B$ (p50, p65), anti-COX-2, anti-iNOS antibodies, respectively. Mori Fructus prevented lipopolysaccharide (LPS)-induced cell death in YPEN cells. Mori Fructus inhibited the generation of $ONOO^-$, NO and ${\cdot}O_2{^-}$ in the LPS-treated cells. Mori Fructus inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-${\kappa}B$ activation. These results suggest Mori Fructus is effective on inhibiting the generation of $ONOO^-$, NO and ${\cdot}O_2{^-}$, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.
Keywords
Mori Fructus; peroxynitrite; nitric oxide; superoxide anion radical; NF-${\kappa}B$; COX-2; iNOS;
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