• Korean Journal of Computational Design and Engineering
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• v.18 no.1
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• pp.71-82
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• 2013

To modify product design easily, modern CAD systems adopt the feature-based model as their primary representation. On the other hand, the boundary representation (B-rep) model is used as their secondary representation. IGES and STEP AP203 edition 1 are the representative standard formats for the exchange of CAD files. Unfortunately, both of them only support the B-rep model. As a result, feature data are lost during the CAD file exchange based on these standards. Loss of feature data causes the difficulty of CAD model modification and prevents the transfer of design intent. To resolve this problem, a tool for recognizing design features from a B-rep model and then reconstructing a feature-based model with the recognized features should be developed. As the first part of this research, this paper presents a method for decomposing a B-rep model into simple volumes suitable for design feature recognition. The results of experiments with a prototype system are analyzed. From the analysis, future research issues are suggested.

• Korean Journal of Computational Design and Engineering
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• v.12 no.3
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• pp.220-232
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• 2007

Current geometric modeling and analysis are commonly based on B-Rep modeling and a finite elements method respectively. Furthermore, it is difficult to represent an object whose material property is heterogeneous using the B-Rep method because the B-Rep is basically used for homogeneous models. In addition, meshes are required to analyze a property of a model when the finite elements method is applied. However, the process of generating meshes from B-Rep is cumbersome and sometimes difficult especially when the model is deformed as time goes by because the topology of deforming meshes are changed. To overcome those problems in modeling and analysis including homogeneous and heterogeneous materials, we suggest a unified modeling and analysis method based on implicit representation of the model using R-function which is suggested by Rvachev. For implicit modeling of an object a distance field is approximated and blended for a complex object. Using the implicit function mesh-free analysis is possible where meshes are not necessary. Generally mesh-free analysis requires heavy computational cost compared to a finite elements method. To improve the computing time of function evaluation, we utilize GPU programming. Finally, we give an example of a simple pipe design problem and show modeling and analysis process using our unified modeling and analysis method.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.118-125
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• 2006

The region responsible for replication of the megaplasmid pAtC58 in the nopaline-type Agrobacterium tumefaciens strain C58 was determined. A derivative ofa Co1E1 vector, pBluscript SK-, incapable of autonomous replication in Agrobacterium spp, was cloned with a 7.6-kb Bg1II-HindIII fragment from a cosmid clone of pAtC58, which contains a region adjacent to the operon for the utilization of deoxyfructosyl glutamine (DFG). The resulting plasmid conferred resistance to carbenicillin on the A. tumefaciens strain UIA5 that is a plasmidfree derivative of C58. The plasmid was stably maintained in the strain even after consecutive cultures for generations. Analysis of nested deletions of the 7.6-kb fragment showed that a 4.3-kb BglII-XhoI region sufficiently confers replication of the derivative of the ColE1 vector on UIA5. The region comprises three ORFs, which have high homologies with repA, repB, and repC of plasm ids in virulent Agrobacterium spp. including pTiC58, pTiB6S3, pTi-SAKURA, and pRiA4b as well as those of symbiotic plasmids from Rhizobium spp. Phylogenie analysis showed that rep genes in pAtC58 are more closely related to those in pRiA4 than to pTi plasmids including pTiC58, suggesting that the two inborn plasmids, pTiC58 and pAtC58, harbored in C58 evolved from distinct origins.

• Journal of the Korean Society for Precision Engineering
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• v.13 no.3
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• pp.158-164
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• 1996

In this paper, the CNC cutting planning module for product with three dimensional solid shape is realized to develop a smart CAD/CAM system which performs systematically from the shape design of procuct by the B-Rep solid modeler to the CNC cutting of product by a machining center. The three dimensional solid shape of product can be easily designed and constructed by the Euler operators and Boolean operators of the solid modeler. And the various functions such as the automatic generation of tool path for the rough and finish cutting processes, the automatic elimination of overcut, the automatic generation of CNC code for the machining center and do on, are established. Especially, the overcut-free tool paths are obtained by splitting the CL solid which is composed of the offset surfaces of the solid shape of product.

• Korean Journal of Computational Design and Engineering
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• v.10 no.4
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• pp.262-273
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• 2005

For CAD data users, few things are as frustrating as receiving CAD data that is unusable due to poor data quality. Users waste time trying to get better data, fixing the data, or even rebuilding the data from scratch from paper drawings or other sources. Most related works and commercial tools handle the boundary representation (B-Rep) shape of CAD models. However, we propose a design history?based approach for healing CAD model errors. Because the design history, which covers the features, the history tree, the parameterization data and constraints, reflects the design intent, CAD model errors can be healed by an interdependency analysis of the feature commands or of the parametric data of each feature command, and by the reconstruction of these feature commands through the rule-based reasoning of an expert system. Unlike other B Rep correction methods, our method automatically heals parametric feature models without translating them to a B-Rep shape, and it also preserves engineering information.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.05a
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• pp.45-45
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• 2004

B-rep 기반의 솔리드 복원 기법은 비교적 복잡한 물체의 경우에도 복원이 잘 되지만, 후보면의 수가 증가함에 따라 탐색 공간 및 시간이 기하급수적으로 늘어나는 단점이 있다. 빈번한 조합 탐색과 복잡한 기하 연산으로 인해 도면이 복잡해질수록 복원 효율성이 떨어지고, 모호성이 발생하는 문제가 있다. 그러나, 이차 곡면을 포함하는 복잡한 물체에 대해서도 복원이 가능하므로 복원 대상 범위가 넓다고 할 수 있다. CSG 기반의 솔리드 복원 기법은 세 투영면에서 돌출 시킨 각각의 솔리드를 서로 교차시켜서 3차원 물체를 복원하는 방법으로, 복잡한 조합 탐색이나 기하 연산 작업을 하지 않게 때문에 비교적 효율적인 복원이 가능하다.(중략)

• Korean Journal of Computational Design and Engineering
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• v.7 no.2
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• pp.121-130
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• 2002

The concept of Level Of Detail (LOD) was introduced and has been used to enhance display performance and to carry out certain engineering analysis effectively. We would like to use an adequate complexity level for each geometric model depending on specific engineering needs and purposes. Solid modeling systems are widely used in industry, and are applied to advanced applications such as virtual assembly. In addition, as the demand to share these engineering tasks through networks is emerging, the problem of building a solid model of an appropriate resolution to a given application becomes a matter of great necessity. However, current researches are mostly focused on triangular mesh models and various operators to reduce the number of triangles. So we are working on the multi-resolution of the solid model itself, rather than that of the triangular mesh model. In this paper, we propose multi-resolution representation of B-rep model by reordering and converting design features into an enclosing volume and subtractive features.

• IMMUNE NETWORK
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• v.2 no.4
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• pp.195-201
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• 2002

Background: IgE is closely related to the development of allergies. However, the poor relationship between the specific IgE level and the severity of allergic diseases suggests the possibility of functionally different IgE isoforms. With this in mind, rat basophilic leukemia (RBL)-2H3 activation was analyzed with each type of rat IgE for two parameters, exocytosis and IL-4 mRNA production. RBL-2H3 has been well documented in the rat mucosal mast cell line. Methods: RBL-2H3 cells sensitized with each kind of rat IgE was activated by cross-linking FcRI with B5 (monoclonal anti-rat IgE mouse IgG antibodies). The RBL-2H3 exocytosis was measured by analyzing the ${\beta}$-hexosaminidase level, and the level of IL-4 mRNA synthesis was analyzed using semiquantitative RT-PCR. Rat IgE, which was produced by a parasite infection (REP), was prepared using either Paragonimus westermani metacercariae (REP-PW) or Anisakis simplex third stage larvae (REP-AS). A rat IgE prototype of IR162 was prepared by a peritoneal injection of immunocytoma. Results: The level of exocytosis showed a linear relationship with the rat IgE concentration when REP-PW or REP-AS was applied. However, it exhibited a biphasic response with IR162. In addition, the time course of heating at $56^{\circ}C$ illustrated the similarity between REP-PW and REP-AS, which differed from that of IR162. In contrast, the level of IL-4 mRNA synthesis in the RBL-2H3 cells with IR162 was comparable to that of either REP-PW or REP-AS. Conclusion: These results suggest that functionally different rat IgE isoforms exists in RBL-2H3 exocytosis.

• Journal of Microbiology and Biotechnology
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• v.10 no.3
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• pp.312-320
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• 2000

Abstract The full sequence of the plasmid pKJ36, which was derived from Bifidobacterium longum KJ, was determined and analyzed to construct shuttle vectors between E. coli and Bifidobacterium. The plasmid pKJ36 was composed of 3,625 base pairs with a 65.1% G+C content. The structural organization of pKJ36 was highly similar to that of pKJ50, and the three major ORFs on pKJ36 showed high amino acid sequence homologies with those of pKJ50. The putative proteins coded by these three ORFs were designated as RepB (32.0 kDa, pI=9.25), MembB (29.0 kDa, pI=12.25), and MobB (39.0 kDa, pI=IO.66), respectively. The amino acid sequence of RepB showed a 57% identity and 70% similarity with that of the RepA protein of pKJ50. Upstream of the repB gene, the so-called iteron sequence was directly repeated four-and-ahalf times and a conserved dnaA box was identified. An amino acid sequence comparison between the MobB and MobA of pKJ50 revealed a 48% identity and 61 % similarity. A conserved oriT sequence with an inverted repeat identical to that of pKJ50 was also found upstream of the mobB gene. A hydropathy analysis of MembB revealed four possible transmembrane regions. The expressions of the repB and membB genes were confirmed by RT-PCR. The in vitro translation reaction of pKJ36 showed protein bands with anticipated sizes with respect to each putative gene product. S 1 endonuclease treatment and Southern hybridization suggested that pKJ36 replicates by a rolling circle mechanism via a single-stranded DNA (ssDNA) intermediate. A shuttle vector between E. coli and Bifidobacterium sp. was constructed using the pKJ36, pBR322, and staphylococcal chloramphenicol acetyl transferase (CAT) gene. The successful transformation of the Bifidobacterium strains was shown by Southern hybridization and PCR. The transformation efficiency differed from strain to strain and, depending on the electroporation conditions, with a range between $1.2{\times}10^1-2.6{\times}10^2{\;}cfu/\mu\textrm{g}$ DNA.X> DNA.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.10a
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• pp.244-248
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• 2001

A solid modeller was developed in this paper. Representation scheme of modelling is nonmanifold B-rep. While many B-rep modelers use edges as basic element of modelling, this modeller is triangle-based. New modelling element, split triangle that is composed of 3 pure half-edges and has no information about the adjoining triangles, was defined to enlarge the range of representation. Corresponding algorithms for Boolean set operations were also developed.