Browse > Article

repABC- Type Replicator Region of Megaplasmid pAtC58 in Agrobacterium tumefaciens C58  

LEE KO-EUN (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
PARK DAE-KYUN (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
BAEK CHANG-HO (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
HWANG WON (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
KIM KUN-SOO (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.1, 2006 , pp. 118-125 More about this Journal
Abstract
The region responsible for replication of the megaplasmid pAtC58 in the nopaline-type Agrobacterium tumefaciens strain C58 was determined. A derivative ofa Co1E1 vector, pBluscript SK-, incapable of autonomous replication in Agrobacterium spp, was cloned with a 7.6-kb Bg1II-HindIII fragment from a cosmid clone of pAtC58, which contains a region adjacent to the operon for the utilization of deoxyfructosyl glutamine (DFG). The resulting plasmid conferred resistance to carbenicillin on the A. tumefaciens strain UIA5 that is a plasmidfree derivative of C58. The plasmid was stably maintained in the strain even after consecutive cultures for generations. Analysis of nested deletions of the 7.6-kb fragment showed that a 4.3-kb BglII-XhoI region sufficiently confers replication of the derivative of the ColE1 vector on UIA5. The region comprises three ORFs, which have high homologies with repA, repB, and repC of plasm ids in virulent Agrobacterium spp. including pTiC58, pTiB6S3, pTi-SAKURA, and pRiA4b as well as those of symbiotic plasmids from Rhizobium spp. Phylogenie analysis showed that rep genes in pAtC58 are more closely related to those in pRiA4 than to pTi plasmids including pTiC58, suggesting that the two inborn plasmids, pTiC58 and pAtC58, harbored in C58 evolved from distinct origins.
Keywords
Agrobacterium tumefaciens; rep genes; plasmid; pAtC58;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
연도 인용수 순위
1 Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215: 403-410   DOI
2 Baek, C.-H. and K.-S. Kim. 2003. lacZ- and aph-based reporter vectors for in vivo expression technology. J. Microbiol. Biotechnol. 13: 872-880
3 Bartosik, D., J. Baj, and M. Wlodarczyk. 1998. Molecular and functional analysis of pTAV320, a repABC-type replicon of the Paracoccus versutus composite plasmid pTAV1. Microbiology 144: 3149-3157   DOI   ScienceOn
4 Dessaux, Y., J. Tempe, and S. K. Farrand. 1987. Genetic analysis of mannityl opine catabolism in octopine-type Agrobaterium tumefaciens strain 15955. Mol. Gen. Genet. 208: 301-308   DOI
5 Gallie, D. R., M. Hagiya, and C. I. Kado. 1985. Analysis of Agrobacterium tumefaciens plasmid pTiC58 replication region with a novel high-copy-number derivative. J. Bacteriol. 161: 1034-1041
6 Jouanin, L. 1984. Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid 12: 91-102   DOI   ScienceOn
7 Kho, D.-H., J.-H. Jang, H.-S. Kim, K.-S. Kim, and J. K. Lee. 2003. Quorum sensing of Rhodobacter sphaeroides negatively regulates cellular poly-${\beta}$-hydroxybutyrate content under aerobic growth conditions. J. Microbiol. Biotechnol. 13: 477-481   과학기술학회마을
8 Ramirez-Romero, M. A., N. E. Soberon, A. Perez-Oseguera, J. M. Tellez-Sosa, and M. A. Cevallos. 2000. Structural elements required for replication and incompatibility of the Rhizobium etli symbiotic plasmid. J. Bacteriol. 182: 3117- 3124   DOI
9 Ramirez-Romero, M. A., J. Tellez-Sosa, H. Barrios, A. Perez-Oseguera, V. Rosas, and M. A. Cevallos. 2001. RepA negatively autoregulates the transcription of the repABC operon of the Rhizobium etli symbiotic plasmid basic replicon. Mol. Microbiol. 42: 195-204   DOI
10 Turner, S. L. and J. P. W. Young. 1995. The replicator region of the Rhizobium leguminosarum cryptic plasmid pRL8JI. FEMS Microbiol. Lett. 133: 53-58   DOI
11 del Solar. G., R. Giraldo, M. J. Ruiz-Echevarria, M. Espinosa, and R. Diaz-Orejas. 1998. Replication and control of circular bacterial plasmids. Microbiol. Mol. Biol. Rev. June: 434-464
12 Kim, K.-S. and S. K. Farrand. 1996. Ti plasmid-encoded genes responsible for catabolism of the crown gall opine mannopine by Agrobacterium tumefaciens are homologs of the T-region genes responsible for synthesis of this opine by the plant tumor. J. Bacteriol. 178: 3275-3284   DOI
13 Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., U.S.A
14 Ramirez-Romero, M. A., P. Bustos, L. Girard, O. Rodriguez, M. A. Cevallos, and G. Davilla. 1997. Sequence, localization and characteristics of the replicator region of the symbiotic plasmid of Rhizobium etli. Microbiology 143: 2825-2831   DOI   ScienceOn
15 Freiberg, C., R. Fellay, A. Bairoch, W. J. Broughton, A. Rosenthal, and X. Perret. 1997. Molecular basis of symbiosis between Rhizobium and legumes. Nature (London) 387: 394-401   DOI   ScienceOn
16 Kim, T., M.-S. Kim, M.-K. Jung, M.-J. Joe, J.-H. Ahn, K.-H. Oh, M. Lee, M. Kim, and J.-O. Ka. 2005. Analysis of plasmid pJP4 horizontal transfer and its impact on bacterial community structure in natural soil. J. Microbiol. Biotechnol. 15: 376-383   과학기술학회마을
17 Nishiguchi, R., M. Takanami, and A. Oka. 1987. Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA4b. Mol. Gen. Genet. 206: 1-8   DOI
18 Cangelosi, G. A., E. A. Best, G. Martinetti, and E. W. Nester. 1991. Genetic analysis of Agrobacterium. Methods Enzymol. 204: 384-397   DOI
19 Papas, K. M. and S. C. Winans. 2003. The RepA and RepB autorepressors and TraR play opposing roles in the regulation of a Ti plasmid repABC operon. Mol. Microbiol. 49: 441-455   DOI   ScienceOn
20 Suzuki, K., N. Ohta, Y. Hattori, M. Uraji, A. Kato, and K. Yoshida. 1998. Novel structural difference between nopaline-and octopine-type trbJ genes: Construction of genetic and physical map and sequencing of trb/traI and rep gene clusters of a new Ti plasmid pTi-SAKURA. Biochem. Biophys. Acta 1396: 1-7   DOI   ScienceOn
21 Baek, C.-H., S. K. Farrand, D. K. Park, K. E. Lee, W. Hwang, and K.-S. Kim. 2005. Genes for utilization of deoxyfructosyl glutamine (DFG), an amadori compound, are widely dispersed in the family Rhizobiaceae. FEMS Microbiol. Ecol. 53: 221-233   DOI   ScienceOn
22 Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70: 191-197   DOI   ScienceOn
23 Tabata, S., P. J. J. Hooykaas, and A. Oka. 1989. Sequence determination and characterization of the replicator region in the tumor-inducing plasmid pTiB6S3. J. Bacteriol. 171: 1665-1672   DOI
24 Van Montagu, M. and J. Shell. 1979. The Plasmid of Agrobacterium tumefaciens. Elsevier/North Holland Biomedical Press, Amsterdam, The Netherlands
25 Rosenberg, C. and C. Huguet. 1984. The pAtC58 plasmid of Agrobacterium tumefaciens is not essential for tumor induction. Mol. Gen. Genet. 196: 533-536   DOI
26 Baek, C.-H., D. K. Park, K. E. Lee, W. Hwang, I. H. Kim, J. Maeng, and K.-S. Kim. 2004. Genes for the catabolism of deoxyfructosyl glutamine in pAtC58 are attributed to utilization of octopine in Agrobacterium tumefaciens strain NT1. J. Microbiol. Biotechnol. 14: 822-828   DOI   ScienceOn
27 Bignell, C. and C. M. Thomas. 2001. The bacterial ParA-ParB partitioning proteins. J. Biotechnol. 91: 1-34   DOI   ScienceOn
28 Simon, R., U. Priefer, and A. Puhler. 1983. A broad host range mobilization for in vivo genetic engineering: Transposon mutagenesis in gram-negative bacteria. Biotechnology NOV: 784-791
29 Palmer, K. M., S. L. Turner, and J. P. W. Young. 2000. Sequence diversity of the plasmid replication gene repC in the Rhizobiaceae. Plasmid 44: 209-219   DOI   ScienceOn
30 Baek, C.-H., S. K. Farrand, K. E. Lee, D. K. Park, J. K. Lee, and K.-S. Kim. 2003. Convergent evolution of amadori opine catabolic systems in plasmids of Agrobacterium tumefaciens. J. Bacteriol. 185: 513-524   DOI
31 Li, P.-L. and S. K. Farrand. 2000. The replicator of the nopaline-type Ti plasmid pTiC58 is a member of the repABC family and is influenced by the TraR-dependent quorum-sensing system. J. Bacteriol. 182: 179-188   DOI
32 Cevallos, M., H. Porta, J. Izquierdo, C. Tun-Garrido, A. García-de-los-Santos, G. Davila, and S. Brom. 2002. Rhizobium etli CFN42 contains at least three plasmids of the repABC family: A structural and evolutionary analysis. Plasmid 48: 104-116   DOI   ScienceOn
33 Van Larebeke, N., G. Engeler, M. Holsters, S. Van den Elsacker, I. Zaenen, R. A. Shilperoort, and J. Shell. 1974. Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability. Nature 252: 169-170   DOI   ScienceOn
34 Bartosik, D., M. Szymanik, and M. Wlodarczyk. 2001. Identification of the partitioning site within the repABC-type replicon of the composite Paracoccus versutus plasmid pTAV1. J. Biotechnol. 183: 6234-6243
35 Sciaky, D., A. L. Montoya, and M. D. Chilton. 1978. Fingerprints of Agrobacterium Ti plasmids. Plasmid 1: 238- 253   DOI   ScienceOn
36 Zhang L., P. J. Murphy, A. Kerr, and M. E. Tate. 1993. Agrobacterium conjugation and gene regulation by N-acyl-$_L$-homoserine lactones. Nature 362: 446-448   DOI   ScienceOn
37 Mercado-Blanco, J. and J. Olivares. 1994. The large nonsymbiotic plasmid pRmeGR4a of Rhizobium melioti GR4 encodes a protein involved in replication that has homology with the RepC protein of Agrobacterium plasmids. Plasmid 32: 75-79   DOI   ScienceOn
38 Rigottier-Gois, L., S. L. Turner, J. P. W. Young, and N. Amarger. 1998. Distribution of repC plasmid-replication sequences among plasmids and isolates of Rhizobium leguminosarum bv. viciae from field populations. Microbiology 144: 771-780   DOI   ScienceOn
39 Soberon, N., T. Venkova-Canova, M. A. Ramirez-Romero, J. Tellez-Sosa, and M. A. Cevallos. 2004. Incompatibility and the partitioning site of the repABC basic replicon of the symbiotic plasmid from Rhizobium etli. Plasmid 51: 203- 216   DOI   ScienceOn
40 Nair, G. R., Z. Liu, and A. N. Binns. 2003. Reexamining the role of the accessory plasmid pAtC58 in the virulence of Agrobacterium tumefaciens strain C58. Plant Physiol. 133: 989-999   DOI   ScienceOn
41 Papas, K. M. and S. C. Winans. 2003. A luxR-type regulator from Agrobacterium tumefaciens elevates Ti plasmid copy number by activating transcription of plasmid replication genes. Mol. Microbiol. 48: 1059-1073   DOI   ScienceOn