• Journal of agricultural medicine and community health
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• v.31 no.3
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• pp.245-253
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• 2006

Objectives: Since 1983, vaccination for Hepatitis B virus has been recommended for all neonates in Korea. The objectives of this study was to assess the changing trend of the positive rate of HBsAg and anti-HBs in university students in one province. Methods: The study population consisted of one university students who taken physical exam in 2004, which included 8,527 students (3,391 males and 5,136 females). Serum HBsAg and anti-HBs were detected by immunochromatography method. Results: The positivities of HBsAg and anti-HBs were 2.7% and 61.1%. The positivity of HBsAg was significantly higher in males(p< .05), while that of anti-HBs was significantly higher in females(p< .05). There was some differences of HBsAg positivities among age groups. But there was no correlation between age and the positivities. There was some differences of anti-HBs positivities among age groups. But there was no correlation between age and the positivities. Conclusion: The positivity of HBsAg has significantly decreased and that of anti-HBs has increased with years. It was suggested that the changes of positivity were caused by nationwide hepatitis B vaccination program.

• The Korean Journal of Nuclear Medicine Technology
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• v.14 no.2
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• pp.203-207
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• 2010

Purpose: Hepatitis B is infection caused by Hepatitis B virus (HBV). Currently, there are several methods, Kits and equipments for conducting Hepatitis B test. Due to ununiformed methods, it would cause some differences. To manage these differences, it needs process evaluating function of test system and reagent using particular standard substance. The aim of this study is to investigate tendency of RIA method's reagent used in Asan Medical Center through comparing several other test reagents using national standard substance. Materials and Methods: The standard substance in National Institute of Food and Drug Safety Evaluation's biology medicine consists of 5 things, 4 antigens and 1 antibody. We tested reagents using A, B company's Kits according to each test method. All tests are measured repeatedly to obtain accurate results. Results: Test result of "HBs Ag Mixed titer Performance panel" is obtained match rate compared S/CO unit standard with RIA method and EIA 3 reagents, CIA 2 reagents is that company A's reagent is 94.4% (17/18), 83.3% (15/18), B is 88.9% (16/18), 77.8% (14/18). Test result of "HBs Ag Low titer Performance panel" is obtain that EIA 2 reagents is shown 7 posive results, CIA 3 reagents is 11, and RIA method's company A's reagent is 3, B is 2 of 13 in low panel. "HBV surface antigen 86.76 IU/vial" tested dilution. A is obtain positive results to 600 times(0.14 IU/mL), B is 300 times (0.29 IU/mL). Case of "HBV human immunoglobulin 95.45 IU/vial", A is shown positive result to 10,000 times (9.5 mIU/mL) and B is 4,000 times (24 mIU/mL). Test result of "HBs Ag Working Standards 0.02~11.52 IU/mL" is shown that Company A's kit concentration level was 0.38IU/mL, company B was 2.23 IU/mL and higher level of concentration was positive results. Conclusion: When comparing various test reagents and RIA method according to National Standard substances for Hepatitis B test, we recognized that there were no significant trends between reagents. For hepatitis B virus antigen-antibody titers even in parts of the test up to 600 times the antigen, antibodies to 10,000 times the maximum positive results could be obtained. Therefore, we confirmed that results from Asan Medical Center are performed smoothly by reagents and system for hepatitis B virus test.

• Environmental Mutagens and Carcinogens
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• v.11 no.2
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• pp.134-140
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• 1991

B형 간염바이러스의 표면항원, 파상풍, 디프테리아균의 toxoid, 백일해의 혼합백신인 PDT-Hepa의 효능을 실험하여 다음과 같은 결과를 얻었다. 시험물질을 기니픽에 접종하였을 때 B형 간염 바이러스의 표면 항원과 파상풍 및 디프테리아균의 toxoid에 대한 항체형성이 뚜렷이 관찰되었으며, 항체형성의 정도는 접종용량에 비례하였다. 백일해의 경우는 시험물질을 마우스에 접종시킨 후 마우스에 감수성이 있는 Bordetella pertussis 18323주로 직접 공격 접종하여 이 균에 대한 방어력이 형성되어 있음을 관찰하였으며, 이러한 방어정도는 투여 용량에 비레함을 알 수 있었다.

• Journal of Preventive Medicine and Public Health
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• v.19 no.1 s.19
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• pp.45-55
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• 1986

It is well known that Korea is one of the endemic area for type B viral hepatitis. A screening test for HBsAg by RPHA method was performed to evaluate the prevalence of HBsAg among urban, rural and coastal area population during the period between Jan. 1985 and Aug. 1985. The results obtained are as follows; 1) The HBsAg was detected in 242 among 2,849 with positive rate of 8.49%. The positive rate by sex showed higher rate in males(9.07%) than females(6.79%) for HBsAg. But these differences by sen were not statistically significant. 2) By living area, the positivity of HBsAg was 8.67% in urban, 7.43% in rural and 8.37% in coastal area. But the differences were not statistically significant. 3) The positivity differences of HBsAg were significantly observed between male(9.26%) and female(6.44%) in urban area. But there was not in rural and coastal area. 4) The positivity was the highest in 4th decade in urban area. But 6th decade in rural and 4th and 5th decade in coastal area. Generally the positivity of HBsAg was more prevalent in young age and decreasing by the age increasing in the male. But in the female it was most common in 6th decade. 5) Elevated SGOT(>40 Unit) was significantly higher in HBsAg positive group(13.6%) than in HBsAg negative group(3.4%).

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.455-460
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• 1986

Dane particle was prepared from the plasma of chronic HBsAg carrier with high levels of HBsAg activity. DNA extracted front Dane particle core after a DNA polymerase reaction with $\alpha$-($^{32}$P) dNTP, was identified as HBV DNA by liquid scintillation counter and agarose gel electrophoresis-G.M. counting. To produce Hepatitis B surface antigen for use as a vaccine against Hepatitis B virus infection, yeast strains harboring recombinant plasmid with Apase promoter was used. Recombinant plasmid was construced from pHBV 130 and pAN 82, transformed into E coli, and then transferred into yeast strains. HBsAg was produced by derepression in Burkholder minimal medium with controlled inorganic phosphate concentration. The kinetics of HBsAg production was also investigated. Total HBsAg activity increased rapidly between 3 and 6 hours after transfer to phosphate-free medium and reached a maximum at around 9th hour. The transfer into phosphate-free medium after 6 hours in high phosphate cell growth medium gave maximum activity.

• Journal of Korean society of Dental Hygiene
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• v.14 no.4
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• pp.563-570
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• 2014

Objectives : The purpose of this study was to investigate the relationship of subjective recognition for oral health in HBsAg positive carriers based on the data of the $5^{th}$ National Health and Nutrition Examination Survey. Methods : A statistical package SPSS 21.0 was used to analyze the relationship between oral health status and subjective oral health awareness based on data of the $5^{th}$ National Health and Nutrition Examination Survey. Results : The subjective poor oral health was found in those who had mastication and speaking discomfort and those having dental prosthesis. Conclusions : The respondents considered themselves to be in poor oral health when the subjective systemic health status and objective oral health status were worse.

• 한국약용작물학회:학술대회논문집
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• 2009.05a
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• pp.239-240
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• 2009

• Korean Journal of Microbiology
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• v.28 no.1
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• pp.6-12
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• 1990

The preS2 wequence of an adr hepatitis B virus was cloned and expressed in Escherichia coli as a $\beta$-galactosidase fusion polypeptide. Recombinant preS2 product interacted with the preS2-specific monoclonal antibody H8 which was induced by surface antigen particles isolated from a Korean gepatitis patient. The H8 showed only a minor cross-reactivity with recombinant preS2 product of adw2 subtype. Determination of nucleotide sequence of the adr preS2 revealed that twelve amino acid residue substitutions between adr and adw2 subtype sequences. The antigenic determinant to H8 must include some of these differences.

• Journal of Yeungnam Medical Science
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• v.13 no.2
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• pp.272-278
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• 1996

Four subtypes of hepatitis B surface antigen are useful in the epidemiologic studies of the route of virus transmission and clinical significance of simultaneous occurance of hepatitis B surface antigen and antibody to hepatitis B surface antigen in the same serum as well as useful marker for population migration. The sera were obtained from 214 HBs Ag positive patients who are diagnosed as chronic liver disease and following up in the Yeungnam university hospital. The subtypes were determined by solid-phase sandwich EIA using monoclonal antibodies. Among 214 specimens, the subtype adr was 93.9%, adw was 2.8%, ayr was 0.9%, ar was 0.9%, adwr was 1.4% and ayw was not detected. There were no correlation between subtype pattern and disease. In summary, the subtype adr was prominent in our study and the difference of subtype pattern by severity of disease was not significant. However, to determine the prognostic value of HBs Ag subtype and relationship between subtype and disease progression, long-term follow up will be needed.

• 대한예방의학회:학술대회논문집
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• 1996.10a
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• pp.377-378
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• 1996