• Asian-Australasian Journal of Animal Sciences
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• 제11권3호
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• pp.311-317
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• 1998

A total of 1033 Brown Swiss and 610 Canadienne cows were phenotyped for the genetic variants ${\alpha}_{s1}$-casein, ${\beta}$-casein, ${\kappa}$-casein, ${\beta}$-lactoglobulin and ${\alpha}$-lactalbumin. In Brown Swiss, frequency distributions were: 97.3% B and 2.7% C variant of ${\alpha}_{s1}$-casein; 31.6% $A^1$, 51.8% $A^2$, 0.5% $A^3$ and 16.1% B variant of ${\beta}$-casein; 70.4% A, 29.3% B, and 0.3% C variant of ${\kappa}$-casein; 41.7% A and 58.3% B variant of ${\beta}$-lactoglobulin; and 100% B variant of ${\alpha}$-lactalbumin. Corresponding frequencies in Canadienne for those five milk proteins were: 98.6 and 1.4%;58.5, 33.5, 0.08 and 7.9%; 78.8, 21.1 and 0.1%, 42.4 and 57.6%; and 100%. Analysis of variance by least squares showed possible association between milk protein phenotypes and some lactational production traits. There were no significant association of phenotypes of ${\alpha}_{s1}$-casein, ${\beta}$-casein and ${\beta}$-lactoglobulin with milk yield, fat yield, protein yield, fat percentage and protein percentage in both breeds during the three lactations. In the Brown Swiss, ${\kappa}$-casein phenotype was associated with 305-day fat yield and protein yield during the first lactation. ${\kappa}$-Casein AB was associated with higher milk, fat and protein yield during the second lactation. During the third lactation, ${\beta}$-lactoglobulin AA in Canadienne cows was associated with higher protein content in the milk (3.70%) when compared to phenotypes AB (3.54%) and BB (3.64%).

• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1275-1277
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• 2013

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.482-490
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• 2001

The gene for glycoprotein B (gB2) of HSV-2-strain G was subcloned, sequenced, recombinated into the lacZ-HcNPV, expressed in insect cells, and compared with the homologous gene of other HSV-2 strains. The ORF of the gB2 gene was 2,715 bp. The overall nucleotide sequence homology of te gB2 gene compared ith that of the two previously reported HSV-2 strains appeared to be over 98%. A recombinant virus named Baculo-gB2 protein in insect cells. The recombination was confirmed by a PCR and the expression was demonstrated by radio immunoprecipitation. Insect cells infected with the Baculo-gB2 virus synthesized and processed gB2 with approximately 120 kDa in the cells, and then secreted it into the culture media, where it reacted with a nomoclonal antibody to gB2. The gB2 polypeptide contained two main hydrophobic regions (a signal sequence from 1 to 23 amino acid residues, and a membrane anchor sequence from aa 745 to 798), eight N-glycosylation sites evenly distributed, and was rich in alanine (11.2%). Antibodies to this recombinant protein that were raised in mice recognized the viral gB2 and neutralized the infectivity of the HSV-2 in vitro. There results show that the gB2 protein was successfully porduced in insect cells and could be used to raise a protective neutralizing antibody. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

• BMB Reports
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• 제32권1호
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• pp.45-50
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• 1999

We previously found three transcription factor-binding motifs in the rat p53 promoter. They are two recognition motifs of NF1-like protein (NF1-like element 1: -296 ~ -312, NF1-like element 2: -195 ~ -219) and a bHLH protein binding element (-142 ~ -146). In this study, we investigated the DNA-protein complex formation of the three elements with nuclear extracts from both normal and regenerating liver to find the element involved in the induced transcription of p53. The level of each DNA-protein complex on NF1-like and bHLH motifs was not changed. Instead, a new element located at -264 ~ -284 was detected in the DNase I footprinting assay with regenerating nuclear extract. This element has partial homology to the AP1 consensus motif. However, the competition studies with diverse oligonucleotides suggest that the binding protein is not AP1. An in vitro transcription assay shows that this element is important for the transcriptional activation of the rat p53 promoter. Therefore, for the induced transcription of the rat p53 promoter, the-264 ~ -284 region is required in addition to two NF1-like and one bHLH motif.

• 한국환경농학회지
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• 제25권1호
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• pp.7-13
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• 2006

비닐하우스는 노지보다 UV-B 선량이 적고 외기와 차단되어 노지와 다른 생육환경 하에서 작물이 생육하게 된다. 본 연구는 UV-B 저선량 상태인 비닐하우스 재배 잎들깨에 UV-B를 처리하여 수량과 생리변화를 검토하고자 수행하였다. UV-B는 형광램프를 이용하여 잎들깨 수관으로부터 150, 120, 90 cm에서 처리하여 각각 노지자연량, 노지자연량+50% 증가량, 노지자연량+100% 증가량을 다른 광의 간섭이 없는 밤 9시부터 2시간씩 30일간 처리하였다. UV-B 처리에 의한 잎들깨 수량은 비닐하우스, 노지 자연량 150% 증가량, 노지자연량, 노지자연량+100% 증가량 처리 순이었다. 7월 20일의 자연 UV-B 선량은 노지 13.6 kJ/일, 비닐하우스 4.9 kJ/일로 비닐하우스의 UV-B 선량은 노지보다 64% 감소되는 것으로 측정되었다. UV-B가 균일하게 처리된 잎들깨의 단백질을 추출하여 2차원전기영동으로 분리하고, 이미지분석하여 발현량을 분석한 결과 UV-B에 의해 33개의 단백질 발현이 변화되었으며 이중 10개가 동정되었다. 동정된 단백질의 기능별로 분류하면 광합성과 관련된 것이 40%, 스트레스 및 스트레스 방어와 관련된 것이 60%였다. UV-B에 의해 발현이 감소한 단백질은 광합성과 관련된 ATP synthase CF1 alpha chain이었고 발현이 증가한 단백질은 DNA recombination and repair protein recF, Heat shock protein 21, Catalase, Galactinol synthase, S-adenosyl-L-methionine, Calcium-dependent protein kinase(CDPK)-like 로 주로 스트레스 및 스트레스 방어와 관련된 단백질들로서 UV-B는 잎들깨 세포내 DNA와 광합성기구를 손상시켜 광합성에 저해적으로 작용하여 수량을 감소시킨 것으로 판단되었다.

• Parasites, Hosts and Diseases
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• 제51권5호
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• pp.497-502
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• 2013

Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cystforming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.256-263
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• 1994

The aceB gene, encoding for malate synthase, one of the key enzymes of glyoxylate bypass, was isolated from a pMT1-based Corynebacterium glutamicum gene library via complementation of an Escherichia coli aceB mutant on an acetate minimal medium. The aceB gene was closely linked to aceA, separated by 598 base pairs, and transcribed in divergent direction. The aceB expressed a protein product of Mr 83, 000 in Corynebacterium glutamicum which was unusually large compared with those of other malate synthases. A DNA-sequence analysis of the cloned DNA identified an open-reading frame of 2, 217 base pairs which encodes a protein with the molecular weight of 82, 311 comprising 739 aminoo acids. The putative protein product showed only limited amino acid-sequence homology to its counteliparts in other organisms. The N-terminal region of the protein, which shows no apparent homology with the known sequences of other malate synthases, appeared to be responsible for the protein s unusually large size. A potential calciumbinding domain of EF-hand structure found among eukaryotes was detected in the N-terminal region of the deduced protein.

• The Korean Journal of Physiology and Pharmacology
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• 제22권1호
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• pp.91-99
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• 2018

Protein phosphatase 1 (PP1) is involved in various signal transduction mechanisms as an extensive regulator. The PP1 catalytic subunit (PP1c) recognizes and binds to PP1-binding consensus residues (FxxR/KxR/K) in NBCe1-B. Consequently, we focused on identifying the function of the PP1-binding consensus residue, $^{922}FMDRLK^{927}$, in NBCe1-B. Using site-directed mutagenesis and co-immunoprecipitation assays, we revealed that in cases where the residues were substituted (F922A, R925A, and K927A) or deleted (deletion of amino acids 922-927), NBCe1-B mutants inhibited PP1 binding to NBCe1-B. Additionally, by recording the intracellular pH, we found that PP1-binding consensus residues in NBCe1-B were not only critical for NBCe1-B activity, but also relevant to its surface expression level. Therefore, we reported that NBCe1-B, as a substrate of PP1, contains these residues in the C-terminal region and that the direct interaction between NBCe1-B and PP1 is functionally critical in controlling the regulation of the ${HCO_3}^-$ transport. These results suggested that like IRBIT, PP1 was another novel regulator of ${HCO_3}^-$ secretion in several types of epithelia.

• 한국식품과학회지
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• 제34권3호
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• pp.407-412
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• 2002

1996년산 한국산 맥주보리의 단백질 함량은 높은 수준으로 조사되었고 지역별, 품종별 단백질 함량의 평균치는 전남산 Doosan 29호 14.1%(d.b), 경남산 Sacheon 6호 13.4%(d.b), 제주산 Doosan 8호 12.8%(d.b)이었으며 전남산 평균치가 14.1%(d.b)인 것은 맥주보리 단백질 함량으로는 매우 높은 수준이었다. 고단백 보리는 저단백 보리에 비하여 분상질이 적고 초자질이 많아 Mealy가 9.0 %인 반면에 Glassy는 54.0%로 높은 수준이었다. 고단백 보리의 곡피함량은 9.9%로 저단백질 보리 7.1% 보다 높았으며 색택 측정치에 있어서 고단백 보리 $51.4^{\circ}L$, 저단백 보리 $55.2^{\circ}L$로 고단백 보리에서 낮게 나타났다. Micromalting을 통하여 고단백질 보리가 발아기간중 맥아품질에 미치는 영향을 조사한 결과, 단백질 함량은 발아기간중 큰 변화가 없었다. 단백질이 높으며 맥아 용해도 즉, friability가 낮아져 발아 6일차 평균치를 기준으로 하면 저단백의 friability는 84.2%인 반면에 고단백은 44.5% 정도에 그쳤다. 맥주보리 단백질이 +1%(d.b) 증가함에 따라 -0.86%(d.b)의 맥아 fine grind extract가 손실되는 것으로 나타나 단백질은 경제적인 측면에 직접적인 손실을 주는 것으로 조사되었으며, 발아 6일차 평균치를 기준시 맥아 fine grind exract가 저단백은 80.8%(d.b)이었으나 고단백은 75.3%(d.b)로 낮았다. 발아 6일차 평균치를 기준시 고단백 맥아는 저단백 맥아 보다 ${\beta}-glucan$의 함량이 약 2.1배 높은 것으로 조사되었고, 단백질이 높으면 맥즙 점도와 맥아 색도가 높아지고, Kolbach index는 떨어지는 것으로 나타났다. 고단백 맥아는 유리 아미노질소가 254.2 ppm이었고 효소역가가 420.6 wk로서, 저단백 맥아에서 각각 187.2 ppm, 267.9 wk인 것 보다 크게 높아, 유리 아미노질소와 diastatic power는 고단백질 맥주 보리의 장점으로 확인되었다. 맥주품질에 큰 영향을 미치는 맥주보리의 단백질 함량은 너무 낮아도 안되지만 특히 높을 경우 여러 측면에서 맥아품질에 부정적인 영향을 주므로 한국산 고단백 보리를 맥주양조용으로 사용할 경우 맥아품질을 개선하기 위한 대책 및 고단백 보리의 근본적인 원인조사와 관리규격을 설정해야 할 것으로 조사되었다.

• Journal of Animal Science and Technology
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• 제48권3호
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• pp.353-360
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• 2006

본 연구는 쥐 난자에서 인위적 활성화 처리가 난자의 활성화, cyclin B1 단백질의 발현 및 난자의 활성화와 cyclin B1 단백질 발현간의 상관관계에 미치는 영향에 관하여 조사하고자 실시하였다. 난자의 활성화 처리는 7% ethanol(EtOH) or 10μg/ml Ca-ionophore with or without 10μg/ml cycloheximide (CH) 방법으로 단일 또는 복합처리 하였다. 난자의 활성화 비율은 단일처리(p<0.05)와 복합처리 (p<0.01)한 난자가 무처리에 비하여 유의하게 높았다. Cyclin B1 단백질의 발현이 EtOH+CH 처리한 난자를 제외한 다른 처리군에서는 무처리에 비하여 유의하게 감소하였다(p<0.05). 한편 EtOH+CH(r=0.61, p<0.05)와 Ca+CH(r=0.86, p<0.01) 처리그룹에서 cyclin B1 단백질의 발현과 난자의 활성화 간에 높은 역상관관계가 있음을 확인하였다. 하지만 단일처리 그룹에서는 두 요소간에 상관관계가 없음을 알 수 있었다. 따라서 단일(EtOH and Ca-ionophore) 또는 복합(EtOH+CH and Ca+CH) 활성화 처리가 난자의 활성화를 증가시키며, 이것은 난자의 활성화 처리에 따른 cyclin B1 단백질의 감소와 밀접한 연관이 있다고 사료된다.