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http://dx.doi.org/10.3347/kjp.2013.51.5.497

Identification of Atg8 Isoform in Encysting Acanthamoeba  

Moon, Eun-Kyung (Department of Parasitology, Kyungpook National University School of Medicine)
Hong, Yeonchul (Department of Parasitology, Kyungpook National University School of Medicine)
Chung, Dong-Il (Department of Parasitology, Kyungpook National University School of Medicine)
Kong, Hyun-Hee (Department of Parasitology, Dong-A University College of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.51, no.5, 2013 , pp. 497-502 More about this Journal
Abstract
Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cystforming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess a type of Atg8. In this study, an isoform of Atg8 was identified and characterized in Acanthamoeba castellanii (AcAtg8b). AcAtg8b protein was found to encode 132 amino acids and to be longer than AcAtg8 protein, which encoded 117 amino acids. Real-time PCR analysis showed high expression levels of AcAtg8b and AcAtg8 during encystation. Fluorescence microscopy demonstrated that AcAtg8b is involved in the formation of the autophagosomal membrane. Chemically synthesized siRNA against AcAtg8b reduced the encystation efficiency of Acanthamoeba, confirming that AcAtg8b, like AcAtg8, is an essential component of cyst formation in Acanthamoeba. Our findings suggest that Acanthamoeba has doubled the number of Atg8 gene copies to ensure the successful encystation for survival when 1 copy is lost. These 2 types of Atg8 identified in Acanthamoeba provide important information regarding autophagy formation, encystation mechanism, and survival of primitive, cyst-forming protozoan parasites.
Keywords
Acanthamoeba castellanii; encystation; autophagy protein 8; isoform;
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