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http://dx.doi.org/10.5187/JAST.2006.48.3.353

Effects of Artificial Stimulations on the activation of oocyte and the expression of cyclin B1 protein in mouse oocytes  

Hwang, S.S. (Dept of Animal Science & Technology, Chung-Ang University)
Kim, C.K. (Dept of Animal Science & Technology, Chung-Ang University)
Chung, Y.C. (Dept of Animal Science & Technology, Chung-Ang University)
Publication Information
Journal of Animal Science and Technology / v.48, no.3, 2006 , pp. 353-360 More about this Journal
Abstract
This study was performed to investigate the effects of artificial stimulation on the increase of the oocyte activation, to evaluate the expression of cyclin B1 protein levels in enucleated mouse oocytes, and to investigate correlation between the oocyte activation and the cyclin B1 protein levels. The oocyte activation was induced by 7% ethanol (EtOH) or 10μg/ml Ca-ionophore with or without 10μg/ml cycloheximide (CH). The activation rate was significantly higher in both single (p<0.05) and combined (p<0.01) stimulated groups compared to control group. The cyclin B1 protein level was significantly reduced in both stimulated groups (p<0.05), except for EtOH+CH treatment group. The expression of cyclin B1 protein showed a higher negative correlation with activation rate in EtOH+CH (r=0.61, p<0.05) and Ca+CH (r=0.86, p<0.01) stimulation groups, but not in a both single stimulation groups. Taken together, it can be suggested that single (EtOH and Ca- ionophore) and combined (EtOH+CH and Ca+CH) stimulation increases the oocyte activation, especially combined stimulation, because it induces the degradation of cyclin B1 protein after artificial stimulation treatments in mouse oocytes.
Keywords
Artificial stimulation; Oocyte activation; Cyclin B1 protein; Mouse;
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