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Analysis of $^1H$ MR Spectroscopy of parietal white matter material Phantom (두정부 백질 물질을 이용한 수소 자기 공명 분광 분석)

  • Lee, Jae-Yeong;Lim, Cheong-Hwan;Kim, Myeong-Soo
    • Journal of radiological science and technology
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    • v.26 no.2
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    • pp.57-61
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    • 2003
  • The purpose of this study is to compare both 1.5T and 4.7T in Praietal White matter material Phantom using the same methodology at both field strengths. Data at both field strengths are compared in terms of $T_2$ relaxation times, line widths and SNRs MR imaging and $^1H$ MR spectroscopy were performed on GE 1.5T SIGNA system and Broker Biospec 4.7T/30 MRI/MRS system. After phantom axial scan $^1H$ MRS was obtained from T2 weighted image by 3-dimensional localization technique(PRESS : Point RE solved spectroscopy Sequence) this phantom is composed of an aqueous solution 36.7 mmol/L of NAA, 25.0 mmol/L of Cr, 6.3 mmol/L of choline chloride, 30.0 mmol/L or Glu, and 22.5 mmol/L of MI(adjusted to a pH of 7,15 in a phosphate buffet). Data processed using software developed inhouse. At 1.5T, T2 relaxation times for Cho, Cr, and NAA were $0.41{\pm}0.07,\;0.26{\pm}0.04,\;0.46{\pm}0.07$ while at 4.7T they were $0.17{\pm}0.03,\;0.14{\pm}0.05,\;0.20{\pm}0.03$ respectively. At 1.5T, line widths for water, Cho, Cr and NAA were $2.9{\pm}0.7,\;1.6{\pm}0.7,\;1.7{\pm}0.8,\;2.2{\pm}0.02Hz$ while at 4.7T they were $5.2{\pm}1.1,\;4.6{\pm}1.9,\;4.01{\pm}1.8,\;4.8{\pm}1.9Hz$ respectively. It can be seen that $T_2$ relaxation times were significantly shorter at 4.7 compared to 1.5T and that the line widths were also broader. The average SNRs for NAA for subjects at short and long TEs were $23.5{\pm}11.3$ at TE=20 msec ; $15.4{\pm}7.7$ at TE=272 msec at 1.5T and $40{\pm}8.3$ and $17{\pm}3.5$ respectively at 4.7T higher field strength is superior because of improved sensitivity and chemical shift dispersion. However these improvements are partially offset by increased line widths and decrease $T_2$ relaxation times, which act to reduce both sensitivity and resolution. In our experiments with the equipment available to us, 4.7T proton spectra at short TEs exhibit moderately improved sensitivity compared to 1.5T.

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Development of Flower Color Changed Landscape Plant through Interspecific and Intergeneric Crosses of Several Cruciferae Crops (십자화과 작물의 종속간 교배를 통한 화색변화 경관용 자원식물 개발)

  • Kim, Kwang-Soo;Park, Won;Lee, Yong-Hwa;Lee, Ji-Eun;Moon, Youn-Ho;Cha, Young-Lok;Song, Yeon-Sang
    • Korean Journal of Plant Resources
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    • v.31 no.1
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    • pp.77-85
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    • 2018
  • The cultivation area of rapeseed (Brassica napus L.) has been increased for oil production and landscaping purpose in Korea. However, as the color of rapeseed flower is very simple, diversified flower color is necessary to improve landscape effect. Interspecific and intergeneric crosses between rapeseed (Brassica napus) and three Cruciferae crops were performed in order to grow diverse flower color of rapeseed. The silique formation rate of interspecific cross rapeseed with cabbage (B. oleracea L) was relatively high (65.8%) and higher than intergeneric cross with rapeseed and radish (Raphanus sativus L.), rapeseed and Orychophragmus, respectively. During silique developing period after artificial pollination, there were many siliques without seeds due to the failure of fertilization. The average number of seed per silique obtained from cross rapeseed and cabbage, rapeseed and radish, rapeseed and O. violaceus were 0.12, 0.4 and 0.12, respectively. The phenotypes of $F_1$ hybrid plants from cross rapeseed and Cruciferae crops were mostly similar to maternal line, but leaf length and leaf width were increased. The interspecific cross of rapeseed and cabbage generated ivory color of flower which is the medium color of parents, and intergeneric cross of rapeseed and O. violaceus created entities with larger flowers which seems to enhance landscape effect. The fatty acid composition of most hybrid seeds intermediated of the two parents for oleic acid, linoleic acid and linolenic acid, content. Whereas hybrid of rapeseed and radish produced less erucic acid than radish parent.

Elevated Temperature Treatment Induced Rice Growth and Changes of Carbon Content in Paddy Water and Soil (온도상승 환경 처리가 논토양과 용수에서 탄소량 변화와 벼 생육에 미치는 영향)

  • Hong, Sung-Chang;Hur, Seung-Oh;Choi, Soon-Kun;Choi, Dong-Ho;Jang, Eun-Suk
    • Korean Journal of Environmental Agriculture
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    • v.37 no.1
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    • pp.15-20
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    • 2018
  • BACKGROUND: The global mean surface temperature change for the period of 2016~2035 relative to 1986~2005 is similar for the four representative concentration pathway (RCP)'s and will likely be in the range of $0.3^{\circ}C$ to $0.7^{\circ}C$. Climate change inducing higher temperature could affect not only crop growth and yield, but also dynamics of carbon in paddy field. METHODS AND RESULTS: This study was conducted to evaluate the effect of elevated temperature on the carbon dynamics in paddy soil and rice growth. In order to control the elevated temperatures, the experiments were set up as the small scale rectangular open top chambers (OTCs) of $1m(width){\times}1m(depth){\times}1m(height)$ (Type 1), $1 m(W){\times}1m(D){\times}1.2m(H)$ (Type 2), and $1m(W){\times}1m(D){\times}1.4m(H)$ (Type 3). The average temperatures of Type 1, Type 2, and Type 3 from July 15 to October 30 were higher than the ambient temperatures at $0.4^{\circ}C$, $0.5^{\circ}C$, and $0.9^{\circ}C$, respectively. For the experiment, Wagner's pots (1/2,000 area) were placed inside chambers. The pots were filled with loamy soil, and chemical fertilizer and organic compost were applied as recommended after soil test. The pots were flooded with agricultural water and rice (Shindongjin-byeo) was planted. It was observed that TOC (total organic carbon) of the water increased by the elevated temperatures and the trend continued until the late growth stage of the rice. Soil TOC contents were reduced by the elevated temperatures. C/N ratios of the rice plant decreased by the elevated temperature treatments. Thus, it was assumed that the elevated temperatures induced to decompose soil organic matter. Elevated temperatures significantly increased the culm length (P<0.01) and culm weight (P<0.05) of rice, but the number and weight of rice panicle did not showed significant differences. CONCLUSION: Based on the results, it was suggested that the elevated temperatures had an effect on changes of soil and water carbons under the possible future climate change environment.

Consideration of the Quantitative Nut Characteristics in Chestnut Hybrids (밤나무 교잡종(交雜種) 과실(果實)의 양적특성(量的特性)에 관한 고찰(考察))

  • Lee, Uk;Kim, Mahn-Jo;Lee, Moon-Ho;Hwang, Myoung-Soo;Hwang, Suk-In
    • Journal of Korean Society of Forest Science
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    • v.94 no.1 s.158
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    • pp.34-38
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    • 2005
  • This study, basic research for releasing new chestnut cultivar, is conducted to consider quantitative nut characteristics according to combinations and individuals In 11 chestnut hybrids. Number of bur on bearing branch(NBB) and nut yield(NY), which showed large difference among combinations, was superior in JO ($Joook{\times}Otanba$) and KO ($Kwangeun{\times}Otanba$) combinations, respectively. Average nut weight (NW) was 21.1g, the highest in EO ($Eunsan{\times}Otanba$) combination. Nut shape which was expressed to the rate of nut height and width, was investigated to the range of 1.13-1.23 in the all combinations, so nut shape of all combinations showed oval type. EO and JO combinations, which were measured to 30.0% and 27.5%, respectively in the percentage with the pericarp split(PPS), were produced more bad fruits than the others. OK ($Otanba{\times}Kwangeun$) combination, 1.7%, was the most excellent in the percentage of polyembryonic nuts(PPN), and the superior combinations which was measured to the below 5%, could be included five combinations. ER and RK combinations, were the highest values, 16.3% and $10.0kg/cm^2$, in soluble solids content(SSC) and kernel hardness (KH), respectively. NBB showed highly positive correlation with NY, but showed highly negative correlation with SSC and NW. NW showed highly positive correlation with PPS, but showed highly negative correlation with SSC and NH. PPS showed highly positive correlation with PPN. Eight individuals such as superior individuals could be selected by selection criteria.

Environmental and Biological Effects at Narodo, in the Southern Water of Korea, on Bloom of Ichthyotoxic Dinoflagellates Cochlodinium polykrikoides (유해성 Cochlodinium polykrikoides 최초 발생에 관한 나로도 해역의 환경학적 특성 및 식물플랑크톤 군집 구조)

  • Cho Eun Seob
    • Journal of Life Science
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    • v.15 no.2 s.69
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    • pp.225-230
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    • 2005
  • The aim of this study was to examine the fluctuation in phytoplankton assemblages with regarding to environmental conditions and nutrients, which were surveyed quarterly over the fours seasons (February, May, July, and October). In turn, an understanding of biological effects should provide insights into a wide range of initiated Cochlodinium blooms in Narodo. Sampling was carried out throughout 2001 on the coasts of Busan (St. 1), Yeosu (St. 2), Narodo (St. 3), Kohung (St. 4), and Kwangdo (St. 5). The maximum surface water temperature was recorded in July, and it ranged from 20 to $22^{\circ}C$. Salinity showed no great variation, which maintained itself in the range of 29-34 psu. The maximum surface salinity was recorded in February, which was about 34 psu. The chlorophyll $\alpha$ concentration of the surface water ranged from 0.01 to $1.3\;{\mu}g\;1^{-1}$. The concentrations of $NH_{4}-N $ were persistently high from February to October; in particular, the peak was observed at St. 1 in February and May (0.15 and $0.19\;{\mu}mol\;1^{-1}$, respectively), while it was detected at St. 2 in July and October (0.22 and $2.2\;{\mu}mol\;1^{-1}$ respectively). Similar trends to those for $NH_4-N $ were observed in the concentrations of $NO_{2}-N$ and $NO_{3}-N$. In contrast to nitrogen, a distinct peak of $NO_{4}-P$ at St. 3, 4, and 5 was observed throughout year $(0.01-0.1\;{\mu}mol\;1^{-1}$ except for October. At St. 1 encounter a peak of cell number of 30,000 and $13{\times}10^3$ cells $1^{-1}$, respectively, in July and October. During the period of this study, the majority of the taxa were diatoms. The dinoflagellates were rather abundant after February, in particular at St. 3, 4, and 5 which attained an abundance of $10\~20\%$without marked fluctuation during the period of this study. At St. 3, the highest average cell width, $178.11\;{\mu}m$, was recorded: the highest cell length, $337.72\;{\mu}m$, was measured in July. Consequently, dinoflagellates bloom in July at Narodo influenced by warm water current are not only associated with a desirable development of cell morphometric characteristics, but also with the health growth of C. polykrikoides. During the period of this study, warm water currents caused an increased water temperature in Narodo, but did not change the amount of nutrients.

Effects of Post Individualities on Treatability of Small Diameter Japanese Larch (Larix Leptolepis) with ACQ and CCA (낙엽송 원주상 소경목의 원목성상과 방부 처리성)

  • Kim, Yeong-Suk;Hong, Soon-Il;Yun, Jeong-Hee
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.1
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    • pp.52-60
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    • 2006
  • This study investigated how wood properties (i.e., annual rings, sapwood, heartwood, and cracks) might affect preservative treatment in Japanese larch (Larix leptolepis) round-wood product. We specially focused on small-diameter (~10 cm diameter) wood that is commercially sold in market. Among 100 wood samples, the groups of sample with 13~16, 17~20 annual rings represented 33 and 27 in each frequency, while 2~3 and 4~5 mm annual ring width accounted for 72 and 68 in frequency. More than a half (54%) of wood samples contained a mix of heartwood and sapwood in its surface. The rest (46%) had only heartwood exposed in the wood surface. A wide range of checks were showed in the wood samples, but the highest frequency was observed in samples with 1~6 surface (1~14 mm in size) checks and 1~4 end-grain (8~14 mm in size) checks in each round-wood sample. Pressure treatment resulted in a wide range of penetration of ACQ (Alkaline Copper Quat) into the wood, showing $4.3{\pm}4.19mm$ penetration in the wood samples contained a mix of heartwood and sapwood in its surface. However preservative treatment was much less effective for the heartwood only wood samples, ranging average 1.3 mm with ACQ and 1.1 mm with CCA (Chromated Copper Arsenate). These penetration results shown in heartwood samples did not meet the penetration standard that is required for H3 by the Korean Forest Service in relation to wood preservation treatment. These low penetration results were not significantly improved even if we incised wood samples to improve treatment effect, showing only small increase of 0.7 mm with ACQ and 0.6 mm with CCA. When preservative treatment was tested with heartwood, penetration of preservatives decrease with increase of annual rings per a cross-section area (r=0.5345). We also found that the length and number of check had no effect on preservative treatment, showing r=0.1301 and r=0.1802, respectively.

Biological characteristics of Scuticociliate with a polymorphic life cycle of ciliate and cyst isolated from cultured olive flounder, Paralichthys olivaceus (양식넙치, Paralichthys olivaceus에서 분리된 Ciliate와 Cyst의 생활사를 갖는 스쿠티카섬모충의 생물학적 특성)

  • Jin, Chang-Nam;Kang, Hyun-Sil;Park, Geun-Tae;Lee, Je-Hee;Lee, Young-Don;Choi, Woo-Bong;Heo, Moon-Soo
    • Journal of fish pathology
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    • v.18 no.1
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    • pp.19-28
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    • 2005
  • Scuticociliatosis has badly settled one of most damaging diseases during the seedling production process of olive flounder. Paralichthys olivaceus in Korea. We isolated a new type of Scuticociliate from flounder. The parasite metamorphoses to ciliate and cyst phases with each other by environmental changes and survive for a relatively long span. The ciliate was measured average 41.8 ${\mu}m$ in length and 21.0 ${\mu}m$ in width, and cyst was 17.0 ${\mu}m$ and 13.5 ${\mu}m$, respectively. Nutritional condition was determined as a major parameter of metamorphosing between ciliate and cyst stages. The ciliate transforms to a cyst stage because of food shortage, and the cyst returns to a ciliate stage with a favorite environmental condition and shows active growth and reproduction. The ciliate multiplied at the maximal density of $2.9 {\times} 10^5 {m\ell}^{-1}$cells in vitro cultivation at $15 ^{\circ}C$temperature using MS BHI medium and bacterial food sources. The ciliate could be proliferated at a 2.5 to $30 ^ \circ}C$ temperature range, pH 6 to 9, and 1 to 55 ppt salinity. Particularly, it survived over one week at $0 ^{\circ}C$temperature showing a high resistance against unfavorable environmental conditions. And the cyst survived for 320 days in the condition of $5 ^{\circ}C$with no feeding, but its survival period was markedly shortened in higher temperature conditions. The chemotherapeutants (formalin and hydrogen peroxide) were clarified as effective chemicals against the ciliate during in vitro trials, but the effect of therapeutants differed in proportion, depending upon the density and the bathing time of chemical compounds.

Effects of Supplemental Lighting of High Pressure Sodium and Lighting Emitting Plasma on Growth and Productivity of Paprika during Low Radiation Period of Winter Season (겨울철 약광기 파프리카의 생육 및 생산성에 대한 고압나트륨 및 Lighting Emitting Plasma 램프의 보광 효과)

  • Lee, Jong-Won;Kim, Ho Cheol;Jeong, Pyeong Hwa;Ku, Yang-Gyu;Bae, Jong Hyang
    • Horticultural Science & Technology
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    • v.32 no.3
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    • pp.346-352
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    • 2014
  • This research was carried out to investigate the effect of supplemental lighting on stable productivity of paprika (Capsicum annuum L.) during low radiation period of winter season. The supplemental lighting sources used in this research were high pressure sodium (HPS) and lighting emitting plasma (LEP). Photosynthetic photon flux density (PPFD) emitted from both lamps decreased as vertical distance from lamp increased. The PPFD of LEP lamps were twice more than that of the HPS lamp per unit distance, but the rate of decreased PPFD of t he LEP per unit distance was higher than that of HPS lamp. And different degrees of PPFD between HPS and LEP lamps by horizontal distance had a smaller degree of difference than by vertical distance at the 100 cm away point. As daily average PPFD measured at the top of the plant under the supplemental lighting during January, the supplemental lighting significantly increased radiation. Radiation of HPS and LEP lighting was 137% and 315% higher than control (without supplemental lighting = sunlight). Air temperature in the top of the plant was not significant different among treatments. HPS and LEP lighting had no effect on increase of flower settings. Leaf length and width with LEP lighting was the longest, photosynthetic was higher than those of other treatments. Supplemental lighting treatments significant increased fruit length and diameter. Especially LEP lighting treatment had a greater effect on fruit length and diameter. In conclusion, LEP lighting treatment during low radiation period greatly affected growth and production of paprika. Further research will be required for the suitable application of LEP lighting in paprika production.

Spawning Behavior and Early Life History of Korea Freshwater Goby (Rhinogobius brunneus) (밀어 (Rhinogobius brunneus)의 산란행동 및 초기생활사)

  • Moon, Woon-Ki;Na, Young-Eun;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.38 no.1 s.110
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    • pp.83-94
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    • 2005
  • The objective of the study was to elucidate early life history of Rhinogobius brunneus, based on laboratory observations and field works, along with its courtship and spawning behaviors. The male and female fish matured were sampled for the lab experiments from Gap-Stream, which is one of the tributaries of Geum-River, Korea. The spawning behaviors and courtship of matured adults had a typical pattern; matured males of Rhinogobius brunneus built up spawning nests and had their own territory before the spawning, and the behavior then triggered the egg spawning of female with active courtship. The females attached elliptic egg mass as a single layer, on the stones below the spawning nest. After the spawning, the egg mass was guarded by males only until the hatching. The size of egg fertilized, measured by microscope was about 1.5${\pm}$0.1mm in length and 0.7${\pm}$0.1 mm in the egg's width. According to the lab experiments conducted under four temperature regime, the lapsed time for the hatching was shortened as temperatures increased in the treatments. On the contrary to the hatching time, the average length of newly hatched larvae showed a reverse relation with water temperature. The larvae, considered a final stage of the early life history was about 3.6 mm in total length for water temperature of $18^{\circ}C$, whereas they were between 3.1 mm and 3.2 mm for over $25^{\circ}C$. This indicates that higher water temperature may decrease the growth rate in the early life history. These results may provide valuable information for the population conservation and ecosystem protection under accelerated water pollution and habitat degradations in Korea.

The Growth Characteristic of the Main Afforestation Species Using the Change of the Annual Ring in Uiseong Area (연륜 변화를 이용한 의성지역 주요 조림수종의 생장특성)

  • Lee Dong-Sup;Kim Dong-Geun;Bea Kwan-Ho;Suh Hyoung-Min
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.4
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    • pp.274-281
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    • 2005
  • The climate of the Uisong Area is wet in the heat of summer and cold and dry in winter, The climate is highly consistent. The typical forest soil of the Uisong Area has an acidity of pH 4.5-5.6. The thickness of soil A layers is thin to an average of 10-20 cm and is a typical brown forest soil type. The growth characteristic of this climate and soil environment is as follows. In the case of Larix leptolepis, it takes 5-9 years to reach 6 cm diameter, The annual growth rate falls off greatly at an age of 17-19 years. The change of annual rings is more apparent on the north slopes compared with the east and northeast slopes. Pinus rigida takes 7-9 years to reach a 6 cm diameter. Annual growth rates of Pinus rigida fall greatly when age reaches 19 years. The change of annual ring growth of Pinus rigida was most apparent in the west and northeast slopes compared with the south and east slopes. In the case of Pinus koraiensis, the change of the annual ring width according to the characteristics of the slope is not important. Pinus koraiensis takes 6 yearsto reach the 6cm diameter, and annual growth rate fell off at age 19 years. In the case of Quercus acutissima, the growth of the valley trees is more stable than for trees grown at the mountain base. Annual growth rate of Quercus autissima fell off 19 years after planting. Ring growth of Robinia pseudo-acasia on northeast slopes appears much like that on northwest slopes. In conclusion, the main silviculture species reaches a 6 cm diameter in 5-9 years of growth, and the annual growth rate begins to fall between years 13 and 19 in the Uisong Area.