• Journal of Microbiology and Biotechnology
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• 제33권7호
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• pp.934-940
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• 2023

Syneilesis palmata (SP) is a traditional medicinal plant. SP has been reported to have anti-inflammatory, anticancer, and anti-human immunodeficiency virus (HIV) activities. However, there is currently no research available on the immunostimulatory activity of SP. Therefore, in this study, we report that S. palmata leaves (SPL) activate macrophages. Increased secretion of both immunostimulatory mediators and phagocytic activity was observed in SPL-treated RAW264.7 cells. However, this effect was reversed by the inhibition of TLR2/4. In addition, inhibition of p38 decreased the secretion of immunostimulatory mediators induced by SPL, and inhibition of TLR2/4 decreased the phosphorylation of p38 induced by SPL. SPL augmented p62/SQSTM1 and LC3-II expression. The increase in protein levels of p62/SQSTM1 and LC3-II induced by SPL was decreased by the inhibition of TLR2/4. The results obtained from this study suggest that SPL activates macrophages via TLR2/4-dependent p38 activation and induces autophagy in macrophages via TLR2/4 stimulation.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2021년도 제64차 하계학술대회논문집 29권2호
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• pp.601-604
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• 2021

본 논문에서는 모바일 디바이스의 카메라로부터 얻은 RGB이미지를 분석하여 장애물을 안정적으로 탐지할 수 있는 프레임워크를 제안한다. 본 논문에서는 장애물을 안정적으로 찾기 위해 RANSAC(Random Sample Consensus)기반의 다중 평면 방식을 이용한 위험감지 시스템을 제안한다. 우리의 접근 방식은 RGB영상으로부터 특징점(Feature point)을 추출하고, 특징점을 분석(Feature point analysis)하여 영상내의 평면을 감지한다. 복잡한 지형으로 인해 생성되는 다수의 평면을 RANSAC을 통해 단일 평면으로 정규화하고, 이로부터 특징점을 분류하기 위한 기준점을 계산한다. 모바일 디바이스의 위치와 회전 제약 없이 효과적으로 기준평면(Reference plane)을 탐색할 수 있고, 영상 내 특징점을 실시간으로 계산한다. 다양한 실험을 통해 기준평면과 장애물과의 거리를 파악하여 장애물을 효과적으로 분류하는 결과를 얻었다. 우리의 기법은 실세계에서의 위험요소를 감지하고 모바일 디바이스 사용자의 안전성 확보에 활용할 수 있을 거라 기대한다.

• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2022년도 제65차 동계학술대회논문집 30권1호
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• pp.377-380
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• 2022

본 논문에서는 모바일 기반 AR 환경에서 RGB카메라로부터 얻은 영상 분석과 DB 기반의 특징점(Feature point) 매칭을 통하여 보다 정확하게 위험 상황을 알려줄 수 있는 프레임워크를 제안한다. 본 논문에서는 RANSAC(Random sample consensus)기반의 다중 평면 방식을 이용한 특징점을 추출하고 분석하여 영상에 존재하는 장애물을 감지한다. RGB카메라로 얻은 영상을 기반으로 장애물을 검출하는 접근법은 영상에 의존하기 때문에 조명에 따른 특징점 검출이 부정확하고, 조명이나 자연광 또는 날씨에 영향을 많이 받기 때문에 어둡거나 흐린 날씨에서는 장애물 검출이 어려워진다. 이 문제를 완화하기 위해 본 논문에서는 DB기반의 특징점 매칭을 통해 조명에 관계없이 장애물을 효율적이고 정확하게 감지한다. 특징점 매칭을 이용하려면 우선 영상에서 특징점이 안정적으로 추출될 수 있는 환경인, 조명이나 자연광이 충분한 환경에서 감지된 장애물 정보를 데이터베이스화 하여 저장한다. 조명이 충분하지 않은 환경에서 사용자가 사전에 저장된 지역에 근접할 경우 특징점 분석이 아닌 DB 기반 특징점 매칭을 통해 위험 요소를 감지한다. 우리의 방법은 조명의 여부의 관계없이 효과적으로 위험을 감지할 수 있기 때문에 다양한 분야에 활용될 수 있다.

• 정보처리학회 논문지
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• 제13권4호
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• pp.181-188
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• 2024

기존의 비속어 탐지 방법들은 의도적으로 변형된 비속어를 식별하는 데 한계가 있다. 이 논문에서는 자연어 처리의 한 분야인 개체명 인식에 기반한 새로운 방법을 소개한다. 우리는 시퀀스 레이블링을 이용한 비속어 탐지 기법을 개발하고, 이를 위해 한국어 악성 댓글 중 일부 비속어를 레이블링하여 직접 데이터셋을 구축하여 실험을 수행하였다. 또한 모델의 성능을 향상시키기 위하여 거대 언어 모델중 하나인 ChatGPT를 활용해 한국어 혐오발언 데이터셋의 일부를 레이블링을 하는 방식으로 데이터셋을 증강하여 학습을 진행하였고, 이 과정에서 거대 언어 모델이 생성한 데이터셋을 인간이 필터링 하는 것만으로도 성능을 향상시킬 수 있음을 확인하였다. 이를 통해 데이터셋 증강 과정에는 여전히 인간의 관리감독이 필요함을 제시하였다.

• 동의생리병리학회지
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• 제18권3호
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• pp.808-818
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• 2004

This study was designed to elucidate the synergistic cytotoxic mechanisms of the cotreatment of Paljintanghabhwajeoghwan (Paljin) and As₂O₃ in human lung cancer cell line, H-460. The combination of Paljin and As₂O₃ synergistically augmented the cytotoxicity of Paljin and As₂O₃ in H-460 cells. The nature of cytotoxicity was revealed as apoptosis which characterized by chromatin condensation and fragmentation in DAPI staining. Mitochodrial membrane potential transition was observed in H-460 cells treated with Paljin and As₂O₃. The apoptotic cytotoxicity of Paljin and As₂O₃ was accompanied by the cleavage of PARP and ICAD. Of note, pro-apoptotic Bak protein was obviously increased. However, the expression of p53 was not affected by the cotreatment of Paljin and As₂O₃. In addition, the expression of DR5 was increased by the cotreatment of Paljin and AS203. This results suggest that the synergistic cytotoxicity of the cotreatment of Paljin and As₂O₃ might be caused by the changes of the expression levels of a lots proteins, such as PARP, ICAD, Bak, DR5, which play pivotal roles in survival or death of cells.

• 디지털콘텐츠학회 논문지
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• 제19권6호
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• pp.1027-1032
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• 2018

최근, e-스포츠, e-게임을 포함하는 e-레저를 위한 새로운 콘텐츠가 요구되고 있다. 이러한 요구로 사람을 추적 대상으로 하는 e-레저용 모바일 AR 연구가 진행되고 있다. e-레저 모바일 AR은 실외환경에서 사용되기 때문에, 원거리에서의 추적 성능이 중요하다. 그러나, snow, snapchat 등과 같은 기존 모바일 AR은 원거리에서 추적 성능이 낮은 단점이 있다. 따라서, 본 논문에서는 실외환경에서의 e-레저 모바일 AR을 제안한다. 제안된 e-레저 모바일 AR은 색상 마커 및 인체비를 이용하여 실외환경(원거리)에서 머리의 위치를 추적하고, 추적된 위치에 가상의 객체를 증강한다. 제안된 e-레저 모바일 AR의 성능은 추적 성능 및 연산 시간의 측정 실험을 통해 검토되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권19호
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• pp.8337-8343
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• 2014

Paclitaxel is one of the best anticancer agents that has been isolated from plants, but its major disadvantage is its dose-limiting toxicity. In this study, we obtained evidence that the active mutant IPP5 ($8-60hIPP5^m$), the latest member of the inhibitory molecules for protein phosphatase 1, sensitizes human cervix carcinoma cells HeLa more efficiently to the therapeutic effects of paclitaxel. The combination of $8-60hIPP5^m$ with paclitaxel augmented anticancer effects as compared to paclitaxel alone as evidenced by reduced DNA synthesis and increased cytotoxicity in HeLa cells. Furthermore, our results revealed that $8-60hIPP5^m$ enhances paclitaxel-induced G2/M arrest and apoptosis, and augments paclitaxel-induced activation of caspases and release of cytochrome C. Evaluation of signaling pathways indicated that this synergism was in part related to downregulation of NF-${\kappa}B$ activation and serine/threonine kinase Akt pathways. We noted that $8-60hIPP5^m$ downregulated the paclitaxel-induced NF-${\kappa}B$ activation, $I{\kappa}B{\alpha}$ degradation, PI3-K activity and phosphorylation of the serine/threonine kinase Akt, a survival signal which in many instances is regulated by NF-${\kappa}B$. Together, our observations indicate that paclitaxel in combination with $8-60hIPP5^m$ may provide a therapeutic advantage for the treatment of human cervical carcinoma.

• 복식
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• 제65권8호
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• pp.50-63
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• 2015

The purpose of this study is to explore the potential of digital graffiti as a new creative tool in fashion through analysis of its expressive features and methods merged in fashion. The results from the analysis of fashion cases using digital graffiti may be summarized as follows. First, 'Mixing of Materials and Non-materials' matched immaterial lights and images with physical clothing materials and space, effectively expressing imaginary spheres that were not expressible with the existing materials. Second, 'Fantastic Storytelling' expressed theme such as 'Sun', 'UFO', 'Science Fiction Film', 'Fantasy Fairy Tale', and 'Universe' through lasers, lights, augmented reality and LED graffiti. Third, 'Convergence with Human and Digital Media' accentuated the active participation of spectators thereby causing human and digital media to interact with each other. Designer's ideas and unexpected responses of spectators realized various digital graffiti effects, which came across by sheer chance. Fourth, 'Utilizing of Site-Specificity' met with the best results when the digital graffiti was expressed in context of a place or location where fashion performance is taking place. Fifth, ' Playfulness by Consumer Participation' intended to induce voluntary participation from consumers by providing the fashion digital graffiti as play tools, and created new fashion digital graffiti or altered existing stereotypes, thereby invoking new visual and tactile experiences. Likewise, today's digital graffiti are emerging in various fields, and the trend of fashion-digital graffiti integration has already created interesting works.

• 동의생리병리학회지
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• 제17권5호
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• pp.1243-1250
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• 2003

This study was designed to elucidate the synergistic cytotoxic mechanisms of the co-treatment of adriamycin and Paljinhangahm-dan in human hepatoma malignant cancer cell line, HepG2. The combination of adriamycin and the ethanol extract of Paljinhangahm-dan synergistically augmented the cytotoxicity of Adriamycin and Paljinhangahm-dan in HepG2 cells. The cytotoxicity of two drugs was revealed as apoptosis characterized by DNA fragmentaton in agarose gel electrophoresis. The apoptotic cytotoxicity of adriamycin and Paljinhangahm-dan was accompanied by the cleavage of procaspase -3 protease and PARP. Of note, anti apoptotic Bcl2 protein was obviously decreased, but Fas was dramatically increased in HepG2 cells co-treated with Adriamycin and Paljinhangahm -dan. In addition, through 2-D gel electorphoresis, we observed that the expression levels of a lot of proteins were obviously changed by the status of drug treatments. This results suggest that the synergistic cytotoxicity of the co-treatment of adriamycin and Paljinhangahm-dan might be caused by the changes of the expression levels of a lot of proteins which play pivotal roles in cell survival or death.

• Asian Pacific Journal of Cancer Prevention
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• 제15권13호
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• pp.5455-5461
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• 2014

Background: Saponins are a major active component for the traditional Chinese medicine, Rubus parvifolius L., which has shown clear antitumor activities. However, the specific effects and mechanisms of saponins of Rubus parvifolius L. (SRP) remain unclear with regard to human chronic myeloid leukemia cells. The aim of this study was to investigate inhibition of proliferation and apoptosis induction effects of SRP in K562 cells and further elucidate its regulatory mechanisms. Materials and Methods: K562 cells were treated with different concentrations of SRP and MTT assays were performed to determine cell viability. Apoptosis induction by SRP was determined with FACS and DAPI staining analysis. Western blotting was used to detect expression of apoptosis and survival related genes. Specific inhibitors were added to confirm roles of STAT3 and AMPK pathways in SRP induction of apoptosis. Results: Our results indicated that SRP exhibited obvious inhibitory effects on the growth of K562 cells, and significantly induced apoptosis. Cleavage of pro-apoptotic proteins was dramatically increased after SRP exposure. SRP treatment also increased the activities of AMPK and JNK pathways, and inhibited the phosphorylation expression level of STAT3 in K562 cells. Inhibition of the AMPK pathway blocked the activation of JNK by SRP, indicating that SRP regulated the expression of JNK dependent oon the AMPK pathway. Furthermore, inhibition of the latter significantly conferred resistance to SRP pro-apoptotic activity, suggesting involvement of the AMPK pathway in induction of apoptosis. Pretreatment with a STAT3 inhibitor also augmented SRP induced growth inhibition and cell apoptosis, further confirming roles of the STAT3 pathway after SRP treatment. Conclusions: Our results demonstrated that SRP induce cell apoptosis through AMPK activation and STAT3 inhibition in K562 cells. This suggests the possibility of further developing SRP as an alternative treatment option, or perhaps using it as adjuvant chemotherapeutic agent for chronic myeloid leukemia therapy.