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Anticancer Effect of Combination with Paljinhangahm-dan and Adriamycin on HepG2 Human Malignant Hepatoma Cell Line  

Baek Eun Ki (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Moon Goo (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Won Jin Hee (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Kim Dong Ung (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Baek Dong Gi (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Yoon Jun Chul (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Song Bong Gil (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Lee Byung Ho (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Park Sang Gu (Department of Internal Medicine, college of Oriental Medicine. Wongkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.5, 2003 , pp. 1243-1250 More about this Journal
Abstract
This study was designed to elucidate the synergistic cytotoxic mechanisms of the co-treatment of adriamycin and Paljinhangahm-dan in human hepatoma malignant cancer cell line, HepG2. The combination of adriamycin and the ethanol extract of Paljinhangahm-dan synergistically augmented the cytotoxicity of Adriamycin and Paljinhangahm-dan in HepG2 cells. The cytotoxicity of two drugs was revealed as apoptosis characterized by DNA fragmentaton in agarose gel electrophoresis. The apoptotic cytotoxicity of adriamycin and Paljinhangahm-dan was accompanied by the cleavage of procaspase -3 protease and PARP. Of note, anti apoptotic Bcl2 protein was obviously decreased, but Fas was dramatically increased in HepG2 cells co-treated with Adriamycin and Paljinhangahm -dan. In addition, through 2-D gel electorphoresis, we observed that the expression levels of a lot of proteins were obviously changed by the status of drug treatments. This results suggest that the synergistic cytotoxicity of the co-treatment of adriamycin and Paljinhangahm-dan might be caused by the changes of the expression levels of a lot of proteins which play pivotal roles in cell survival or death.
Keywords
Paljinhangahm-dan(八珍抗癌丹); adriamycin; anticancer effect;
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1 韓醫學的 診斷類型에 따른 韓藥方劑의 抗癌 및 免疫調節作用에 미치는 影響 /
[ 안규석(외) ] / 東醫病理學會誌
2 Apoptosis in the immune system /
[ Williams, G.T. ] / J. Patho   ScienceOn
3 Programmed cell death: apoptosis and oncogenesis /
[ Williams, G.T. ] / Cell   ScienceOn
4 Chemotherapy- induced apoptosis /
[ Mesner, P.;Budihardjo, I.;Kaufmann, S.H. ] / Adv. Pharmacol
5 Analysis by matrix assisted laser desorption/ionisation- time of flight mass spectrometry of the post-translational modifications of alpha 1-antitrypsin isoforms separated by two-dimensional polyacrylamide gel electrophoresis /
[ Mills, P.B.;Mills, K.;Johnson, A.W.;Clayton, P.T.;Winchester, B.G. ] / Proteomics.   ScienceOn
6 Human ICE/CED-3 protease nomencla ture /
[ Alnemri, E.S.;Livingstone, D.J.;Nicholson, D.W.;Salvesen, G.;Thornberry, N.A.;Wong, W.W.;Yuan, J. ] / Cell   ScienceOn
7 Dopamine induces apoptosis through an oxidation- involved SAPK/JNK activation pathways /
[ Luo, Y.;Umegaki, H.;Wang, X.;Abe, R.;Roth, G.S. ] / J Biol Chem   ScienceOn
8 A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells /
[ Han, J.;Lee, J.D.;Bibbs, L.;Ulevitch, R.J. ] / Science
9 Chemotherapy- induced apop tosis /
[ Mesner, P.;Budihardjo, I.;Kaufmann, S.H. ] / Adv. Pharmacol.
10 Caspase-dependent Cleavage of signaling proteins during apoptosis /
[ Widmann, C.;Gibson, S. ] / J. Biol. Chem.   ScienceOn
11 Nitric oxide inhibits apoptosis by preventing increase in caspase 3-like activity via two distinct mechanisms /
[ Kim, Y.M.;Talanian, R.V.;Billiar, T.R. ] / J. Biol. Chem
12 Sequential activation of ICE-like and CPP32-like protease during Fas-mediated apoptosis /
[ Enari, M.;Talanian, R.V.;Wong, W.W.;Nagata, S. ] / Nature   ScienceOn
13 Bcl2 and the regulation of programmed cell death /
[ Reed. J.C. ] / J. Cell. Biol   ScienceOn
14 八珍湯合化積丸의 抗腫瘍效果에 관한 연구 /
[ 김성진(외) ] / 大韓韓方內科學會誌
15 八珍湯合化積丸과 Adriamycin의 병용처리시 나타나는 syner gistic 抗腫瘍 效果에 관한 작용기전 연구 /
[ 이병구(외) ] / 大韓韓方內科學會誌
16 Palgin sensitizes the adriamycin- induced apoptosis via the enhancement of Fas/Fas ligand expression /
[ Lim, Y.S.;So, H.S.;Kim, M.S.;Moon, G.;Won, J.H.;Baek, S.W.;Moon, S.R.;Yang, S.H.;Kim, B.J.;Ko, C.B.;Park, R. ] / Life Sci   ScienceOn
17 /
[ 서울대학교의과대학 ] / 인간생명과학
18 /
[ 瀋敏求(외) ] / 中華腫瘤治療大成
19 八珍抗癌丹의 인간 前骨髓性 白血病 細胞 死滅 效果 /
[ 이건업 ] / 원광대 박사학위 논문
20 八珍抗癌丹의 급성 骨髓性 白血病 細胞에서 抗腫瘍 效果 /
[ 백동기 ] / 원광대 석사학위 논문
21 /
[ 대한병리학회 ] / 병리학
22 /
[ 홍원식(역) ] / 現代中國의 癌治療法
23 /
[ 최승훈 ] / 東醫腫瘍學
24 癌腫의 病因病理에 關한 文獻的 考察 /
[ 전병욱(외) ] / 大韓韓方腫瘍學會誌
25 /
[ 김창종 ] / 病態生理學
26 /
[ 홍원식(편) ] / 精校 黃帝內經靈樞
27 景岳全書 /
[ 張介賓 ] / 文淵閣四庫全書 第七七七冊
28 癌의 中藥治療 /
[ 厲暢 ] / 東洋醫學
29 /
[ 이천 ] / 醫學入門
30 /
[ 李家康(외) ] / 中醫腫瘤防治大全
31 /
[ 劉春安(외) ] / 抗癌中草藥大事典
32 /
[ 常敏毅 ] / 抗癌本草
33 Apoptosis required for maintenance of homeostasis : Familial hemophagocytic lymphohistiocytosis caused by too little cell death /
[ Fadeel, B.;Henter, J.I.;Orrenius, S. ] / Lakar- tidningen
34 Programed cell death and the control of cell survival /
[ Raff, M.C.;Bares, B.A.;Burne, J.F.;Coles, H.S.;Ishizaki, Y.;Jacobson, M.D. ] / Science
35 /
[ 문구(외) ] / 암 동서의 결합치료 1
36 New approaches to proteomic analysis of breast cancer /
[ Wulfkuhle, J.D.;McLean, K.C.;Paweletz, C.P.;Sgroi, D.C.;Trock, B.J.;Steeg, P.S.;Petricoin, E.F. 3rd ] / Proteomics   ScienceOn
37 /
[ 홍원식(편) ] / 精校 黃帝內經素問
38 /
[ 서울대학교 의과대학 ] / 腫瘍學
39 /
[ 이문호(외) ] / 내과학
40 /
[ 楊寶仁 ] / 癌症的中醫治療
41 /
[ 홍원식 ] / 黃帝內經素問
42 益氣消症方治療晩期腫瘤臨床硏究 /
[ 李萍萍 ] / 中醫雜誌
43 君理湯加減方이 抗腫瘍 免疫反應과 抗癌劑로 誘發한 副作用에 미치는 影響 /
[ 유경태(외) ] / 大韓韓方腫瘍學會誌
44 消積白朮散이 Bleomycin의 副作用減少와 抗癌效果에 미치는 영향 /
[ 김태운(외) ] / 大韓韓方腫瘍學會誌
45 Apoptosis in the pathogenesis and treatment of disea se /
[ Thomson, C.B. ] / Science
46 /
[ 문준전(외) ] / 東醫病理學
47 /
[ 上海中醫學院 ] / 實用中醫內科學
48 /
[ 신민교 ] / 臨床本草學