• Korean Journal of Medicinal Crop Science
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• v.11 no.4
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• pp.268-273
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• 2003

To identify the variation of the RAPD patterns between two Atractylodes species, 52 kinds of random primers were applied to each eight of A japonica and A. macrocephala genomic DNA. Ten primers of 52 primers could be used to discriminate between the species and 18 polymorphisms among 67 scored DNA fragments (18 fragments are specific for A. japonica and A. macrocephala) were generated using these primers, 26.9% of which were polymorphic. RAPD data from the 10 primers was used for cluster analysis. The cluster analysis of RAPD markers showed that the two groups are genetically distinct. On the other hand, to identify the variation of the AFLP patterns and select the species specific AFLP markers, eight combinations of EcoRI/MseI primers were applied to the bulked A. japonica and A. macrocephala genomic DNA. Consequently, three combinations of EcoRI/MseI primers (EcoRI /Mse I ; AAC/CTA, AAC/CAA, AAG/CTA) used in this study revealed 176 reliable AFLP markers, 42.0% of which were polymorphic. 74 polymorphisms out of 176 scored DNA fragments were enough to clearly discriminate between two Atractylodes species.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.596-598
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• 2004

The effects of water extract from Atractylodes macrocephala Koidz on biological activity were investigated. The crude water extract of A. macrocephala inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (3 mm inhibition zone at 300 ㎍/disc). However, it did not show growth inhibition activity against Sreptococcus mutans JC-2 (MIC >1,000 ㎍/mL). This extract was cytotoxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC/sub 50/ 62.24 ㎍/mL at 150 ㎍/disc). These results suggest that water extract of A. macrocephala possesses antitumoral, and antimicrobial activities.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.10b
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• pp.40-41
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• 2000

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.04a
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• pp.113-114
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• 2003

• Korean Journal of Medicinal Crop Science
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• v.26 no.3
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• pp.220-226
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• 2018

Background: Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz. belong to the family (Asteraceae). Their rhizomes, called white Atractylodes rhizomes, are used in traditional medicine. To address some issues with their cultivation, we recently developed eight hybrid cultivars by interspecific hybridization of A. japonica and A. macrocephala. This study was conducted to screen the hybrid cultivars that have high amounts of active ingredients and yield ability. Methods and Results: Experiments were conducted using the eight hybrid cultivars and A. macrocephala (control cultivar) in the experimental field of the department of Herbal Crop Research located in Eumseong, South Korea. We investigated the growth characteristics of the aerial and underground parts. Among the cultivars, 'Sanwon' had the highest rhizome dry weight (53.8 g/plant), followed by 'Dachul' (50.0 g/plant). In addition, the content of atractylenolide I, II, III and total active ingredients were investigated using high-performance liquid chromatography. Compared with A. macrocephala, most of the inter-specific hybrid cultivars had a higher content of active ingredients and yield ability. Conclusions: Through study, we established the superior quality of Atractylodes inter-specific hybrid cultivars. In particularly, it was found that 'Dachul' may be grown as a superior cultivar, with high amount of active ingredients as well as yield ability.

• Korean Journal of Plant Resources
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• v.12 no.4
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• pp.276-281
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• 1999

This study was conducted to determine the appropriate transplanting time for growing one year old rhizome of Atractylodes macrocephala Koidz. Transplanting on March 20 and April 5 showed the highest in plant height and shoot fresh weight but dry rhizome weight was the highest in the transplanting time of April 5. As the growth time of 209 days showed was the highest yield of dry rhizome. As a result, it seemed that the appropriate planting time was April 5 to 10 in the north area of the Kyongsangbuk-do. The shortest emergence time was that the accumulated temperature of early growth period was 267$^{\circ}C$, and that of highest dry rhizome yield was 3$600^{\circ}C$ for whole growth period.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.183-188
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• 2004

This study was conducted to investigate the effects on the growth and yield by the different seeding methods and cultivating root weight in Atractylodes macrocephata Koidz. Seeding distances were different such as 5${\times}$5 cm, 5${\times}$10 cm, 10${\times}$10 cm, 10${\times}$15 cm by the hand seeder and 15 cm seeding in drill. Emergence date, plant height were not significantly changed with seeding space, but the number of leaf, fresh weight of above-ground part and fresh weight of root were increased in the sparse seeding compared with the dense seeding. The highest fresh root yield was 1,012 kg/10 a at the 5${\times}$5 cm. Roots yield was increased in the sparse seeding compared with the dense seeding, but the highest number of roots above 16 g yield was observed at the 10${\times}$10 cm seeding distance. Emergence date was faster 1∼3 day root weight above 6 g than that root weight 5 g. The plant height, number of stem and fresh weight of above-ground part were more increased as the root weight was heavier. The growth of underground part were more increased as the root weight was heavier, yield was increased about 27％ to 112％ compared with root weight above 6 g than that 134.6 kg/10 a with root 5 g.

• Food Science and Biotechnology
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• v.27 no.6
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• pp.1823-1831
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• 2018

This study examined the efficacy of Atractylodes macrocephala Koidz (AMK) protein and polysaccharide extracts as adjuvant or adjuvant booster when given together with porcine pleuropneumonia vaccine. Experimental mice (n = 5/group) were subcutaneously immunized with $25{\mu}g$ ApxIIA #3 antigen, a target protein against A. pleuropneumoniae, together with alum and/or various concentrations ($0-500{\mu}g$) of the AMK extracts, while the control group received PBS only. Immunization with ApxIIA #3 antigen increased the antigen-specific IgG titer and this increase was enhanced in the immunization together with AMK protein, but not polysaccharide extract. Supplementation of AMK protein extract exhibited dose-dependent increases in the antigen-induced protective immunity against A. pleuropneumoniae challenge and in the lymphocyte proliferation specific to the antigen. Glycoproteins present in the AMK extract were the active components responsible for immune response induction. Collectively, the present findings suggest that AMK glycoproteins are useful as immune stimulating adjuvant or adjuvant booster.

• Korean Journal of Medicinal Crop Science
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• v.8 no.3
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• pp.216-224
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• 2000

This study was conducted to investigate the soil mulching effect on growth and yield of Atractylodes macrocephala Koidz. The number of stem, stem diameter, and fresh weight of aerial part in black poly-ethylene film and transparent poly-ethylene film mulched condition were higher than those of non-mulching and rice-straw mulching. Number of flowers were high in the order of non-mulching, rice-straw, trans-P. E. film, and black P. E. film. Yield of underground part was higher in mulching condition than that of non-mulching. Dry rhizome yield in black P. E. film mulching was significantly high to compare with the other treatments. There was lower disease incidence of Phytophthora blight in trans-P. E and black P. E. film compare with the other treatments but any difference was not observed in essential oil content of rhizome. Significant positive correlation was found between rhizome weight and fresh weight of aerial part. It was also shown that the fresh weight of aerial part was greatly dependent on the number of stems and stem diameter.

• Korean journal of food and cookery science
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• v.22 no.4 s.94
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• pp.458-467
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• 2006

This study was carried out to determine the functional works and basic ingredients of Sagoonja-Tang on white pan bread. The -experimental groups consisted of 6% mixtures of five(Ed- confirm the number) kinds powders, : B1 for Sagoonja-Tang powder, B2 for Panax ginseng, B3 for Poria cocos, Koidz, B4 for Atractylodes macrocephala, and B5 for Glycyrrhiza uralensis Fisch. The volume of white pan bread somewhat decreased after adding the ingredients, and the volume differences in order from highest to lowest, were white pan bread control>B2>B3>B4>B1>B5. For white pan bread, pH of control, B3, and B5 decreased on the 2nd day during storage, but increased on the 4th and 7th days. In the texture analyses of white pan bread, B4 and B5 showed a higher degree of hardness than that of control, while B2, and B3 were the lowest. In sensory test, the overall acceptability of regular white pan bread in order from highest to lowest was control>B3>B2>B1 ${\cdot}$ B5>B4.