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http://dx.doi.org/10.1007/s10068-018-0430-1

Glycoproteins isolated from Atractylodes macrocephala Koidz improve protective immune response induction in a mouse model  

Kim, Kyoung-A. (Institute of Oral Biosciences and School of Dentistry, Chonbuk National University)
Son, Young-Ok (Cell Dynamics Research Center and School of Life Sciences, Gwangju Institute of Science and Technology)
Kim, So-Soon (Department of Bioactive Material Sciences, Research Center of Bioactive Materials and Institute of Oral Bioscience, Chonbuk National University)
Jang, Yong-Suk (Department of Bioactive Material Sciences, Research Center of Bioactive Materials and Institute of Oral Bioscience, Chonbuk National University)
Baek, Young-Hyun (Institute of Oral Biosciences and School of Dentistry, Chonbuk National University)
Kim, Chun-Chu (Institute of Oral Biosciences and School of Dentistry, Chonbuk National University)
Lee, Jeong-Hoon (Institute of Oral Biosciences and School of Dentistry, Chonbuk National University)
Lee, Jeong-Chae (Institute of Oral Biosciences and School of Dentistry, Chonbuk National University)
Publication Information
Food Science and Biotechnology / v.27, no.6, 2018 , pp. 1823-1831 More about this Journal
Abstract
This study examined the efficacy of Atractylodes macrocephala Koidz (AMK) protein and polysaccharide extracts as adjuvant or adjuvant booster when given together with porcine pleuropneumonia vaccine. Experimental mice (n = 5/group) were subcutaneously immunized with $25{\mu}g$ ApxIIA #3 antigen, a target protein against A. pleuropneumoniae, together with alum and/or various concentrations ($0-500{\mu}g$) of the AMK extracts, while the control group received PBS only. Immunization with ApxIIA #3 antigen increased the antigen-specific IgG titer and this increase was enhanced in the immunization together with AMK protein, but not polysaccharide extract. Supplementation of AMK protein extract exhibited dose-dependent increases in the antigen-induced protective immunity against A. pleuropneumoniae challenge and in the lymphocyte proliferation specific to the antigen. Glycoproteins present in the AMK extract were the active components responsible for immune response induction. Collectively, the present findings suggest that AMK glycoproteins are useful as immune stimulating adjuvant or adjuvant booster.
Keywords
Adjuvant; ApxII toxin; Glycoproteins; Immunoglobulin; Oriental traditional medicine;
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1 Bao LD, Ren XH, Ma RL, Wang Y, Yuan HW, Lv HJ. Efficacy of Artemisia annua polysaccharides as an adjuvant to hepatitis C vaccination. Genet. Mol. Res. 14: 4957-4965 (2015)   DOI
2 Baudner BC, Giuliani MM, Verhoef JC, Rappuoli R, Junginger HE, Giudice GD. The concomitant use of the LTK63 mucosal adjuvant and of chitosan-based delivery system enhances the immunogenicity and efficacy of intranasally administered vaccines. Vaccine 21: 3837-3844 (2003)   DOI
3 Chen K, Cerutti A. Vaccination strategies to promote mucosal antibody responses. Immunity 33: 479-491 (2010)   DOI
4 Del Giudice G, Podda A, Rappuoli R. What are the limits of adjuvanticity? Vaccine 20 (Suppl 1): S38-S41 (2001)   DOI
5 Holmgren J, Czerkinsky C. Mucosal immunity and vaccines. Nat. Med. 11 (4 Suppl): S45-S53 (2005)   DOI
6 Huber VC, Arulanandam BP, Arnaboldi PM, Elmore MK, Sheehan CE, Kallakury BV, Metzger DW. Delivery of IL-12 intranasally leads to reduced IL-12-mediated toxicity. Int. Immunopharmacol. 3: 801-809 (2003)   DOI
7 Ji GQ, Chen RQ, Zheng JX. Macrophage activation by polysaccharides from Atractylodes macrocephala Koidz through the nuclear factor-${\kappa}B$ pathway. Pharm. Biol. 53: 512-517 (2015)   DOI
8 Kim SH, Jung HN, Lee KY, Kim J, Lee JC, Jang YS. Suppression of Th2-type immune response-mediated allergic diarrhea following oral administration of traditional Korean medicine: Atractylodes macrocephala Koidz. Immunopharmacol. Immunotoxicol. 27: 331-343 (2005)   DOI
9 Kim SH, Lee KY, Kim J, Park SM, Park BK, Jang YS. Identification of a peptide motif enhancing mucosal and systemic immune responses against the peptide conjugated antigen after oral administration in mice. Mol. Cells 21: 244-250 (2006)
10 Lee JC, Lee KY, Jung HN, Kim JG, Jang YS. Glycoproteins contained within Soamsan, a traditional Oriental medicine, are the main class of active ingredients responsible for the medicineinduced immune stimulation. J. Biochem. Mol. Biol. 38: 253-257 (2005)
11 Lee JC, Lee KY, Son YO, Choi KC, Kim J, Kim SH, Chung GH, Jang YS. Stimulating effects on mouse splenocytes of glycoproteins from the herbal medicine Atractylodes macrocephala Koidz. Phytomedicine 14: 390-395 (2007)   DOI
12 Podda A, Del Giudice G. MF59-adjuvanted vaccines: increased immunogenicity with an optimal safety profile. Expert Rev. Vaccines 2: 197-203 (2003)   DOI
13 Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J. Immunol. 136: 2348-2357 (1986)
14 Ott G, Barchfeld GL, Van Nest G. Enhancement of humoral response against human influenza vaccine with the simple submicron oil/ water emulsion adjuvant MF59. Vaccine 13: 1557-1562 (1995)   DOI
15 Pi CC, Chu CL, Lu CY, Zhuang YJ, Wang CL, Yu YH, Wang HY, Lin CC, Chen CJ. Polysaccharides from Ganoderma formosanum function as a Th1 adjuvant and stimulate cytotoxic T cell response in vivo. Vaccine 32: 401-408 (2014)   DOI
16 Seo KW, Kim DH, Kim AH, Yoo HS, Lee KY, Jang YS. Characterization of antigenic determinants in ApxIIA exotoxin capable of inducing protective immunity to Actinobacillus pleuropneumoniae challenge. Immunol. Invest. 40: 465-480 (2011)   DOI
17 Seo KW, Kim SH, Park J, Son Y, Yoo HS, Lee KY, Jang YS. Nasal immunization with major epitope-containing ApxIIA toxin fragment induces protective immunity against challenge infection with Actinobacillus pleuropneumoniae in a murine model. Vet. Immunol. Immunopathol. 151: 102-112 (2013)   DOI
18 Wang R, Zhou G, Wang M, Peng Y, Li X. The metabolism of polysaccharide from Atractylodes macrocephala Koidz and its effect on intestinal microflora. Evid. Based Complement. Alternat. Med. 2014: 926381 (2014)
19 Song HP, Li RL, Zhou C, Cai X, Huang HY. Atractylodes macrocephala Koidz stimulates intestinal epithelial cell migration through a polyamine dependent mechanism. J. Ethnopharmacol. 159: 23-35 (2015)   DOI
20 Stevceva L, Strober W. Mucosal HIV vaccines: where are we now? Curr. HIV Res. 2 (1): 1-10 (2004)   DOI