• 대한방사성의약품학회지
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• 제6권1호
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• pp.20-26
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• 2020

Accelerator mass spectrometry (AMS) is a powerful detection technique with the exquisite sensitivity and high precision compared with other traditional analytical techniques. Accelerator mass spectrometry can be widely applied in the technique of radiocarbon dating in the fields of archeology, geology and oceanography. The ability of accelerator mass spectrometry to measure rare ¹⁴C concentrations in microgram and even sub-microgram amounts suggests that extension of ¹⁴C-accelerator mass spectrometry to biomedical field is a natural and attractive application of the technology. Drug development processes are costly, risky, and time consuming. However, the use of ¹⁴C-accelerator mass spectrometry allows absorption, distribution, metabolism and excretion (ADME) studies easier to understand pharmacokinetics of drug candidates. Over the last few decades, accelerator mass spectrometry and its applications to preclinical/clinical trials have significantly increased. For accelerator mass spectrometry analysis of biological samples, graphitization processes of samples are important. In this paper, we present a detailed sample preparation procedure to apply to graphitization of biological samples for accelerator mass spectrometry.

• 분석과학
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• 제5권2호
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• pp.235-238
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• 1992

• 한국환경보건학회지
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• 제21권3호
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• pp.87-95
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• 1995

A rapid and selective co-extraction systems of copper and zinc-thiocyanate complex into various types of alkylamine for the simultaneous determination of two metal ions by atomic absorption spectrometry and ion chromatograph have been proposed. The quantitative extractions of Cu(II) and Zn(II) at 0.1 M-thiocyanate and 0.1 M-HCI were achieved with Aliquat 336-$CHCl_3$. The detection limits of Cu and Zn were 2 ppb and 0.9 ppb respectively.

• 생약학회지
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• 제13권3호
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• pp.102-105
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• 1982

Nine trace elements in Bear Galls(Fel Ursi) studied in this paper are Na, Mg, K, Ca, Cu, Zn, Fe, Pb and Ge. The contents of Na, Mg, K, Ca, Cu, Zn, Fe, Pb are determined by atomic absorption spectrometry and Ge is determined by graphite atomic absorption spectrometry. In both methods, the sample is digested with nitric-perchloric acid and then ashed. The results of analysis are shown in table and the result obtained from this study are as follows. a. The highest content of trace element of Bear Gall is Na and other elements are getting lower in order of K, Mg, Ca, Ge, Zn, Fe, Cu, and Pb. b. Particularly the content of Ge in Bear Gall was determined.

• 한국미생물·생명공학회지
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• 제36권3호
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• pp.221-226
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• 2008

효모에서 사람의 H-, L-ferritin을 생산하기 위해서, 기존에 복제된 vector를 사용하였으며,단백질을 발현하기 위해서 각각의 증폭된 ferritin 유전자를 GALI promoter에 의해 조절되는 pYES2.1/V5-His-TOPO 효모 발현 vector에 삽입하였다. Western blot 분석을 통해서 사람의 H-, L-ferritin subunits을 함유한 재조합 효모에서 사람의 ferritin이 발현된 것을 확인할 수 있었다. 또한 Atomic absorption spectrometry(AAS) 분석을 통해서 형질변환된 효모의 철 함유량이 대조군과 비교하여 $1.6{\sim}l.8$배 증가한 것을 확인하였다. 향후 ferritin이 함유된 형질변환 효모를 사용하여 잠재적으로 철이 강화된 영양성분을 기능성 식품과 사료에 이용할 수 있을 것이다.

• 분석과학
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• 제12권2호
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• pp.130-135
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• 1999

쌀분말시료중에 미량으로 존재하는 비소(As), 납(Pb), 셀레늄(Se)등을 분석하기 위하여 매질변형방법에 의한 흑연로원자흡수분광법을 이용하였다. 흑연로내에서 측정원소의 열안정성과 간섭매질의 휘발성을 증가시키기 위해 매질변형재로서 팔라듐과 질산마그네슘 혼합물을 사용하였다. 몇가지 금속원소(As, Cd, Cu, Pb, Se, Zn)에 대해 팔라듐과 질산마그네슘 혼합물에 의한 매질변형효과를 검토한 결과 As, Pb, Se에 대해 뚜렷한 회화 및 원자화 온도의 증가와 흡수도 상승효과를 확인 하였다. 마이크로파 산가압분해법에 의해 쌀분말시료를 분해하고 표준물첨가법에 의해 As, Pb, Se을 분석하였다. 바탕선 보정에는 Zeeman effect를 이용하였으며 특성농도는 건조시료기준 As 26 ng/g, Pb 18 ng/g, Se 24 ng/g이었다.

• 분석과학
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• 제8권1호
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• pp.69-77
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• 1995

원자흡수용 gas-jet assisted RF 글로우 방전시스템을 제작하고 시료손실량과 원자흡광도에 미치는 실험변수들에 관한 연구를 수행하였다. 시료손실량과 흡광도는 방전전력, 압력 및 가스흐름에 큰 영향을 받았다. 원자흡광도와 시료손실량을 가스흐름이 600ml/min로 증가할 때 까지 계속 증가하였으며, 방전 압력은 3mbar에서 최대 흡광도를 나타내었다. 방전전력이 증가할수록 시료손실량과 흡광도는 증가하였으나 시스템의 안정도에 영향을 미치는 관계로 적당한 값의 선택이 필요하였다. 스테인리스 스틸 시료 중의 Ni과 황동시료 중의 Cr의 검량선을 작성한 결과 양호한 직선성이 얻어졌다.

• 대한화학회지
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• 제43권4호
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• pp.418-422
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• 1999

Cr(III)과 Cr(VI)이온 각각을 Aliquat 336으로 단계적으로 용매추출하여 분리하고 원자흡수분광법으로 정량하였다. 즉, $Cr^{3+}$와 $Cr_2O_7^{2-}$의 혼합용액으로부터 Aliquat 336/MIBK로 먼저 $Cr_2O_7^{2-}$을 선택적으로 추출하고, 그 추출액 중의 농도를 $N_2O-C_2H_2$ 불꽃으로 측정한 다음, 남은 수용액에 citrate를 가하여 $Cr^{3+}$을 음의 착이온으로 만들어 같은 추출제를 사용하여 같은 방법으로 추출 및 정량하였다. $Cr^{3+}$와 $Cr_2O_7^{2-}$의 농도가 Cr로서 각각 0.1∼1.0 g/mL 범위의 혼합시료 50mL부터 1%(V/V) Aliquat 336/MIBK 5 mL로 1회씩 추출하여 각 농도에 대한 분석결과, 회수율이 98.8∼101.7%였다.

• Bulletin of the Korean Chemical Society
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• 제32권3호
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• pp.829-835
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• 2011

A dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) has been developed as a new approach for the extraction of trace copper in water and beverage samples followed by the determination with flame atomic absorption spectrometry. In the DLLME-SFO, 8-hydroxy quinoline, 1-dodecanol, and methanol were used as chelating agent, extraction solvent and dispersive solvent, respectively. The experimental parameters related to the DLLME-SFO such as the type and volume of the extraction and dispersive solvent, extraction time, sample volume, the concentration of chelating agent and salt addition were investigated and optimized. Under the optimum conditions, the enrichment factor for copper was 122. The method was linear in the range from 0.5 to $300\;ng\;mL^{-1}$ of copper in the samples with a correlation coefficient (r) of 0.9996 and a limit of detection of $0.1\;ng\;mL^{-1}$. The method was applied to the determination of copper in water and beverage samples. The recoveries for the spiked water and beverage samples at the copper concentration levels of 5.0 and $10.0\;ng\;mL^{-1}$ were in the range between 92.0% and 108.0%. The relative standard deviations (RSD) varied from 3.0% to 5.6%.

• Bulletin of the Korean Chemical Society
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• 제23권4호
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• pp.545-549
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• 2002

A simple and rapid technique for the separation and preconcentration of lead in water and biological samples has been devised. Preconcentrationis based on the depositionof analyte onto a column packed with dithizone immobilized on sodium dodecyl sulfate coated alumina at pH $\geq$ 3. The trapped lead is eluted with 5 mL of 4 M nitric acid and determined by flame atomic absorption spectroscopy. A sample of 1 L, results in a preconcentration factor of 200 and the precision at 20${\mu}g$ $L^{-1}$ is 1.3%(n=8). The procedure is applied to tap water, well water, river water, vegetable extract and milk samples, and accuracy is assessed through recovery experiments and by independent analysis by furnace atomic absorption.