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Expression of Human Heavy-Chain and Light-Chain Ferritins in Saccharomyces cerevisiae for Functional Foods and Feeds  

Han, Hye-Song (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Lee, Joong-Lim (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Park, Si-Hong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Kim, Jae-Hwan (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Kim, Hae-Yeong (Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.3, 2008 , pp. 221-226 More about this Journal
Abstract
To produce human ferritins in yeast, human H-chain and L-chain ferritins were amplified from previously cloned vectors. Each amplified ferritin gene was inserted into the pYES2.1/V5-His-TOPO yeast expression vector under the control of the GAL1promoter. Western blot analysis of the recombinant yeast cells revealed that H-and L-chain subunits of human ferritin were expressed in Saccharomyces cerevisiae. Atomic absorption spectrometry (AAS) analysis demonstrated that the intracellular content of iron in the ferritin transformant was 1.6 to 1.8-fold higher than that of the control strain. Ferritin transformants could potentially supply iron-fortified nutrients for food and feed.
Keywords
Atomic absorption spectrometry; human ferritin; iron; Saccharomyces cerevisiae;
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