• Title/Summary/Keyword: AtPR4

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Selection of Primula malacoides Lines with Different Growth and Flowering Characteristics (생장이 다양한 Primula malacoides 순계 선발)

  • Song, Cheon Young;Lee, Jeong Ho
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.152-157
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    • 2009
  • To obtain pure lines for breeding $F_1$ varieties of Primula malacoides, 10 lines that were considered uniform in growth and flowering characteristics were selected from self-crossings of the sixth generation ($S_6$). Plant height of the selected lines ranged from 20.8 to 33.2 cm, and number of leaves ranged from 33.6 to 54.2. The selected lines flowered at 181.4 to 207.8 days after sowing. Three lines, including 'PR-03-81', flowered in less than 190 days from sowing, while six lines, including 'PR-03-228', only flowered after more than 200 days from sowing. In terms of petal color, six lines, including 'PR-03-81', were pink, three, including 'PR-03-236', were purple, and line 'PR-03-275' was white. Flower diameter ranged from 2.4 to 2.9 cm, and number of pedicels per plant was between 15.4 and 26.4. Number of flowers per pedicel ranged from 20.2 to 30.2. Germination was more than 70%, and number of seeds per capsule (pod) ranged from 110.0 to 162.8. In summary, plant height was low and flower diameter was small and plants had many flowers and readily formed seeds. The selected lines are not only valuable as ornamental plants but could also be used as breeding materials for $F_1$ hybrids of Primula polyantha.

MAGNETO-OPTICAL KERR SPECTRA FOR AMORPHOUS RE-Co ALLOY FILMS (비정질 RE-Co 합금막의 자기광학 스펙트럼)

  • Y. J. Choe;S. Tsunashima;S. Uchiyama
    • Journal of the Korean Magnetics Society
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    • v.4 no.2
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    • pp.154-159
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    • 1994
  • The magneto-optical Kerr spectra(${\lambda}=250~700nm$) of amorphous RE-Co(RE=Ce, Nd, Pr, Gd, Tb, Er, Ho) are compared with those of Y-Co films. It has been found that the Kerr rotation of RE-Co is mainly due to Co in the long wavelength region, whereas at short wavelength Ce, Pr, Nd, and Gd contribute positively, and Tb, Ho, and Er contribute negatively to the Kerr rotation of RE-Co amorphous films. In the interesting energy region(1.5~5.0 eV), the magneto-optical contribution of Pr and Nd are thought to be related with $4f{\uparrow}->5d{\uparrow}$ interband transition, and the contribution of Ce and Gd might be concerned with d->p interband transition. The magneto-optical effect of Tb in the short wavelength region might be related with $4f{\downarrow}->5d{\downarrow}$ and/or $5d{\downarrow}->4f{\downarrow}$, and that of Ho and Er can be explained by $4f{\downarrow}->5d{\downarrow}$ magneto-optical interband transition.

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Evaluation of Pepper Genetic Sources (Capsicum spp.) for Disease Resistance Breeding (병저항성 육종을 위한 고추 유전자원의 저항성 평가)

  • Lee, Sang-Jun;Kim, Byung-Sup
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.185-191
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    • 2012
  • For initiation of resistance breeding program of the red pepper, 21 PR ($Phytophthora$ resistance) cultivars, 36 cultivars collected from USA and 'Supermanitta' which is a susceptible cultivar against phytophthora blight were assayed against phytophthora blight, powdery mildew, and anthracnose. For seedling assay of phytophthora blight, three different mating type strains of $Phytophthora$ $capsici$ were used (A1, A2, Sterile). The result showed that most of the pepper of PR cultivars were resistance or moderately resistance at each mating type. 'Yeokganghongjanggun' was resistant to all three $P.$ $capsici$ strains and 'PR-Datta' and 'PR-Manitta' were resistant or moderately resistant at each type. In case of the collected cultivars, 'NuMex J.E.Parker', 'Omni Color', and 'SCM334' were resistant to all the three types and some cultivars including 'Sweet Banana' and 'Tabasco' were moderately resistant to each type fungi. 'Orange Habanero' and 'Black Cuban' were resistant to powdery mildew and 'Supermanitta' and 'PR Keumdong' were moderately resistant, while 'Santa Fe Grande', 'NuMex Pinata' and 'Puya' were very susceptible. In the case of anthracnose, 'Aji Limon' and 'Capsicum baccatum var. pendulum 3-4' were resistant and 'Pobalno', 'Omni Color', 'Negro', 'Mesilla', 'Mulato', 'Bhut Jolokia', 'Big Dipper', 'Black Cuban', 'NuMex Pinata', and 'NuMex Big Jim' were moderately resistant. The most PR cultivars except 'Taesan' were susceptible or very susceptible. These resistant individuals identified through this experiment can be used as sources of resistance to pepper pathogens in the future breeding programs.

Effect of Incorporation Rate of Polyacrylamide Hydrogel on Changes in Physical Properties of Root Media (Polyacrylamide 고흡수성 수지의 혼합 비율이 상토의 물리성에 미치는 영향)

  • Wang, Hyun-Jin;Choi, Jong-Myung;Lee, Jong-Suk
    • Journal of Bio-Environment Control
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    • v.14 no.3
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    • pp.182-189
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    • 2005
  • This research was conducted to determine physical properties of four root media, peatmoss + vermiculite (1:1, v/v; PV), peatmoss + composted rice hall (1:1, PR), peatmoss + composted saw-dust (1:1 : PS) and peatmoss + composted pine bark (1:1 PB), as influenced by incorporation rate of Stock-sorb C (STSB). Each root medium containing STSB was packed in 22 cm diameter plastic pot and the physical properties were determined at 5 weeks after packing. As incorporation rate of STSB were elevated, total porosity increased in PV, PS and PB media with statistical differences at $5{\%}$ level. Those also resulted in increase of container capacity in PS and PB media, but statistical differences were not observed in PV and PR media. Elevated incorporation rate of STSB in PV, PS and PB media resulted in increase of air space with statistical differences. Trends in air space of the three root media showed a linear as well as quadratic responses to STSB contents of media. As incorporation rate of STSB increased, more water was retained in four root media at the soil moisture tension of 4.90 kPa, 9.81 kPa, 29.4 kPa and 1.5 MPa. The amount of water retained in PS medium was the highest at the moisture tension at 29.4 kPa and 1.5 MPa followed by PB, PR and PV medium. These results indicated that elevation of incorporation rate of STSB to various root media increased moisture retention capacity, but did not increase the available water holding capacity.

Isolation and Characterization of Pathogenesis-Related Protein 5 (PgPR5) Gene from Panax ginseng

  • Kim, Yu-Jin;Lee, Jung-Hye;Jung, Dae-Young;Sathiyaraj, Gayathri;Shim, Ju-Sun;In, Jun-Gyo;Yang, Deok-Chun
    • The Plant Pathology Journal
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    • v.25 no.4
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    • pp.400-407
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    • 2009
  • A pathogenesis-related protein (PgPR5) gene that isolated from the leaf of Panax ginseng was characterized. The ORF is 756 bp with a deduced amino acid sequence of 251 residues. The calculated molecular mass of the matured protein is approximately 27.5 kDa with a predicated isoelectric point of 7.80. A GenBank BlastX search revealed that the deduced amino acid of PgPR5 shares highest sequence similarity to PR5 of Actinidia deliciosa (80% identity, 87% similarity). PgPR5 has a C-terminal and N-terminal signal peptide, suggesting that it is a vacuolar secreted protein. The expression of PgPR5 under various environmental stresses was analyzed at different time points using real-time PCR. Our results reveal that PgPR5 is induced by salt stress, chilling stress, heavy metal, UV, and pathogen infection. These results suggest that the PgPR5 could play a role in the molecular defence response of ginseng to abiotic and pathogen attack. This is the first report of the isolation of PR5 gene from the P. ginseng.

Synthesis and characterization of CaZrO3:Pr3+ phosphor by skull melting method (스컬용융법에 의한 CaZrO3:Pr3+ 형광체 합성 및 특성분석)

  • Choi, Hyunmin;Kim, Youngchool;Seok, Jeongwon
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.31 no.5
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    • pp.228-232
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    • 2021
  • Ca1-xZrO3:xPr phosphor with perovskite structure was successfully synthesized by using skull melting method. The crystal structure, morphology and optical properties of synthesized phosphor were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), ultraviolet fluorescence reaction and photoluminescence. The XRD results indicated that single crystals of CaZrO3:Pr3+ belongs to orthorhombic perovskite system. The synthesized phosphor could be excited by UV light (254 nm) and the emission spectra results indicated that green luminescence of CaZrO3:Pr3+ due to charge transfer transition 3P03H4, 3P13H5 and 3P03H5 at 506, 536 and 548 nm was dominant.

Study on variation of electrical properties of polymer thick film resistor regarding curing temperature, printing process and substrate (경화 온도와 인쇄 공정 및 기판에 따른 폴리머 후막 저항체의 특성 변화에 대한 연구)

  • Yoo, Myong-Jae;Lee, Sang-Myong;Park, Seong-Dae;Lee, Woo-Sung;Kang, Nam-Kee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2005.07a
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    • pp.311-312
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    • 2005
  • Applying a designed test coupon pattern for fabricating resistors various resistors were formed using PTF(polymer thick film) pastes. Aspect ratio from 0.25 to 4 were selected for fabricating resistors. Formed resistors were cured at $170^{\circ}C$ and $240^{\circ}C$. Electrical properties of fabricated resistors were measured and their values analyzed in relation to cure temperature and formed geometry via printing. Also effects of substrates used for fabricating resistors were observed.

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Kinetics and Mechanism of Substitution Reaction of $PPN^+(\eta^n-MeCp)Mn(CO)_2\;Cl^-$ with $PR_3$(R=Me, Et, OEt, $C_6H_5$)

  • Park, Yong Gwang;Lee, Yong Gu;Kim, Gyu Sik
    • Bulletin of the Korean Chemical Society
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    • v.17 no.2
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    • pp.138-142
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    • 1996
  • The transition metal carbonylate, PPN+(${\eta}^5-MeCp)Mn(CO)_2Cl^-$ undergoes a novel ligand substitution reaction with PR3 (R=Me, Et, OEt, $C_6H_5$ in THF at elevated temperatures (40 $^{\circ}C$ up to 60 $^{\circ}C)$ under the pseudo-first-order reaction conditions (usually 20-fold excess of PR3 with respect to metal carbonylate concentrations) where chloride is displaced by PR3. The reaction follows overall first order dependence on [(${\eta}^5-MeCp)Mn(CO)_2Cl^-$]; however, the negative entropy changes of activation (${\Delta}S^{\neq}$=-19.3 e.u. for $P(OEt)_3$; ${\Delta}S^{\neq}$=-16.4 e.u. for $PPh_3$) suggest the existence of the intermediate, ((η3-MeCp)Mn(CO)2(THF)Cl-, which eventually transforms to the product (${\eta}^5-MeCp)Mn(CO)_2(PR_3)$.

Seismic response and energy dissipation in partially restrained and fully restrained steel frames: An analytical study

  • Reyes-Salazar, Alfredo;Haldar, Achintya
    • Steel and Composite Structures
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    • v.1 no.4
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    • pp.459-480
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    • 2001
  • The damage suffered by steel structures during the Northridge (1994) and Kobe (1995) earthquakes indicates that the fully restrained (FR) connections in steel frames did not behave as expected. Consequently, researchers began studying other possibilities, including making the connections more flexible, to reduce the risk of damage from seismic loading. Recent experimental and analytical investigations pointed out that the seismic response of steel frames with partially restrained (PR) connections might be superior to that of similar frames with FR connections since the energy dissipation at PR connections could be significant. This beneficial effect has not yet been fully quantified analytically. Thus, the dissipation of energy at PR connections needs to be considered in analytical evaluations, in addition to the dissipation of energy due to viscous damping and at plastic hinges (if they form). An algorithm is developed and verified by the authors to estimate the nonlinear time-domain dynamic response of steel frames with PR connections. The verified algorithm is then used to quantify the major sources of energy dissipation and their effect on the overall structural response in terms of the maximum base shear and the maximum top displacement. The results indicate that the dissipation of energy at PR connections is comparable to that dissipated by viscous damping and at plastic hinges. In general, the maximum total base shear significantly increases with an increase in the connection stiffness. On the other hand, the maximum top lateral displacement $U_{max}$ does not always increase as the connection stiffness decreases. Energy dissipation is considerably influenced by the stiffness of a connection, defined in terms of the T ratio, i.e., the ratio of the moment the connection would have to carry according to beam line theory (Disque 1964) and the fixed end moment of the girder. A connection with a T ratio of at least 0.9 is considered to be fully restrained. The energy dissipation behavior may be quite different for a frame with FR connections with a T ratio of 1.0 compared to when the T ratio is 0.9. Thus, for nonlinear seismic analysis, a T ratio of at least 0.9 should not be considered to be an FR connection. The study quantitatively confirms the general observations made in experimental results for frames with PR connections. Proper consideration of the PR connection stiffness and other dynamic properties are essential to predict dynamic behavior, no matter how difficult the analysis procedure becomes. Any simplified approach may need to be calibrated using this type of detailed analytical study.

Cellular Prion Protein Enhances Drug Resistance of Colorectal Cancer Cells via Regulation of a Survival Signal Pathway

  • Lee, Jun Hee;Yun, Chul Won;Lee, Sang Hun
    • Biomolecules & Therapeutics
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    • v.26 no.3
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    • pp.313-321
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    • 2018
  • Anti-cancer drug resistance is a major problem in colorectal cancer (CRC) research. Although several studies have revealed the mechanism of cancer drug resistance, molecular targets for chemotherapeutic combinations remain elusive. To address this issue, we focused on the expression of cellular prion protein ($PrP^C$) in 5-FU-resistant CRC cells. In 5-FU-resistant CRC cells, $PrP^C$ expression is significantly increased, compared with that in normal CRC cells. In the presence of 5-FU, $PrP^C$ increased CRC cell survival and proliferation by maintaining the activation of the PI3K-Akt signaling pathway and the expression of cell cycle-associated proteins, including cyclin E, CDK2, cyclin D1, and CDK4. In addition, $PrP^C$ inhibited the activation of the stress-associated proteins p38, JNK, and p53. Moreover, after treatment of 5-FU-resistant CRC cells with 5-FU, silencing of $PrP^C$ triggered apoptosis via the activation of caspase-3. These results indicate that $PrP^C$ plays a key role in CRC drug resistance. The novel strategy of combining chemotherapy with $PrP^C$ targeting may yield efficacious treatments of colorectal cancer.