• Title/Summary/Keyword: Asymmetric division

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First Report on Hirsutella subulata, a Pathogen of Rice Stem Borer, Chilo suppressalis in Korea (국내 미기록 이화명충 병원사상균(Hirsutella subulata)에 관한 보고)

  • Yoon, Cheol-Sik;Kim, Jeong-Jun;Lee, Min-Ho;Yun, Tae-Yu;Yoo, Jae-Ki
    • The Korean Journal of Mycology
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    • v.27 no.3 s.90
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    • pp.206-207
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    • 1999
  • Hirsutella subulata (Hyphomycetes) was observed for the first time on diseased larvae of Chilo suppressalis in Korea. This fungus formed slender and slightly attenuated synnemata consisting of closely bound, longitudinal hyphae on the insect larvae. Typical characteristics of this species are ellipsoid or broadly obovoid phialides with slender, needle-like necks and asymmetric conidia. It is very similar to H. barberi but can be distinguished by its asymmetrical conidia.

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Development of Audio Melody Extraction and Matching Engine for MIREX 2011 tasks

  • Song, Chai-Jong;Jang, Dalwon;Lee, Seok-Pil;Park, Hochong
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2012.07a
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    • pp.164-166
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    • 2012
  • In this paper, we proposed a method for extracting predominant melody of polyphonic music based on harmonic structure. Harmonic structure is an important feature parameter of monophonic signal that has spectral peaks at the integer multiples of its fundamental frequency. We extract all fundamental frequency candidates contained in the polyphonic signal by verifying the required condition of harmonic structure. Then, we combine those harmonic peaks corresponding to each extracted fundamental frequency and assign a rank to each after calculating its harmonic average energy. We run pitch tracking based on the rank of extracted fundamental frequency and continuity of fundamental frequency, and determine the predominant melody. For the query by singing/humming (QbSH) task, we proposed Dynamic Time Warping (DTW) based matching engine. Our system reduces false alarm by combining the distances of multiple DTW processes. To improve the performance, we introduced the asymmetric sense, pitch level compensation, and distance intransitiveness to DTW algorithm.

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Overexpression, Crystallization, and Preliminary X-Ray Crystallographic Analysis of the Alanine Racemase from Enterococcus faecalis v583

  • Priyadarshi, Amit;Lee, Eun-Hye;Sung, Min-Woo;Kim, Jae-Hee;Ku, Min-Je;Kim, Eunice Eun-Kyeong;Hwang, Kwang-Yeon
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.55-58
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    • 2008
  • Alanine racemase, a bacterial enzyme belonging to the fold-type III group of pyridoxal 5'-phosphate (PLP)-dependent enzymes, has been shown to catalyze the interconversion between L- and D-alanine. The alanine racemase from the pathogenic bacterium Enterococcus faecalis v583 has been overexpressed in E. coli and was shown to crystallize an enzyme at 295 K, using polyethylene glycol (PEG) 8000 as a precipitant. X-ray diffraction data to $2.5{\AA}$ has been collected using synchrotron radiation. The crystal is a member of the orthorhombic space group, $C222_1$ with unit cell parameter of a=94.634, b=156.516, $c=147.878{\AA},\;and\;{\alpha}={\beta}={\gamma}=90{\AA}$. Two or three monomers are likely to be present in the asymmetric unit, with a corresponding $V_m\; of\;3.38{\AA}^3\;Da^{-1}\;and\;2.26{\AA}^3\;Da^{-1}$ and a solvent content of 63.7% and 45.5%, respectively.

Investigation of an Arc-induced Long Period Fiber Grating Inscribed in a Photonic Crystal Fiber with Two Large Air Holes

  • Kim, Sun-Duck;Kim, Gil-Hwan;Hwang, Kyu-Jin;Lim, Sun-Do;Lee, Kwan-Il;Kim, Sang-Hyuck;Lee, Sang-Bae
    • Journal of the Optical Society of Korea
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    • v.13 no.4
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    • pp.428-433
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    • 2009
  • A photonic crystal fiber with two large air holes outside the holey cladding region is fabricated to induce an effective long periodic grating (LPG) in the core by an electric arc discharge. We believe that the two large air holes lead to the asymmetric perturbation in the core under the electric arc discharge, thereby introducing the coupling to the first higher-order mode. The transmission characteristics of the PCF with the LPG for the external perturbation such as strain, curvature, and temperature are also investigated. It was found that the shift of resonance peak in the transmission spectrum depends on the bending direction. The curvature of 8.55 $m^{-1}$ results in the center wavelength shifts of 1.8, 4.3, and 11 nm for a vertical, diagonal, and horizontal direction of the curvature to the large air-hole alignment, respectively.

Contribution of thermal-hydraulic validation tests to the standard design approval of SMART

  • Park, Hyun-Sik;Kwon, Tae-Soon;Moon, Sang-Ki;Cho, Seok;Euh, Dong-Jin;Yi, Sung-Jae
    • Nuclear Engineering and Technology
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    • v.49 no.7
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    • pp.1537-1546
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    • 2017
  • Many thermal-hydraulic tests have been conducted at the Korea Atomic Energy Research Institute for verification of the SMART (System-integrated Modular Advanced ReacTor) design, the standard design approval of which was issued by the Korean regulatory body. In this paper, the contributions of these tests to the standard design approval of SMART are discussed. First, an integral effect test facility named VISTA-ITL (Experimental Verification by Integral Simulation of Transients and Accidents-Integral Test Loop) has been utilized to assess the TASS/SMR-S (Transient and Set-point Simulation/Small and Medium) safety analysis code and confirm its conservatism, to support standard design approval, and to construct a database for the SMART design optimization. In addition, many separate effect tests have been performed. The reactor internal flow test has been conducted using the SCOP (SMART COre flow distribution and Pressure drop test) facility to evaluate the reactor internal flow and pressure distributions. An ECC (Emergency Core Coolant) performance test has been carried out using the SWAT (SMART ECC Water Asymmetric Two-phase choking test) facility to evaluate the safety injection performance and to validate the thermal-hydraulic model used in the safety analysis code. The Freon CHF (Critical Heat Flux) test has been performed using the FTHEL (Freon Thermal Hydraulic Experimental Loop) facility to construct a database from the $5{\times}5$ rod bundle Freon CHF tests and to evaluate the DNBR (Departure from Nucleate Boiling Ratio) model in the safety analysis and core design codes. These test results were used for standard design approval of SMART to verify its design bases, design tools, and analysis methodology.

Primer RNA Synthesis by E. coli RNA Polymerase on the SSB-coated 229-nt ssi Signal of Lactococcal Plasmid pGKV21 (Lactococcal plasmid pGKV21의 SSB-coated 229-nt ssi signal 상에서 E. coli RNA polymerase에 의한 시발체 RNA 합성)

  • Jeong, Jin-Yong;Kim, Eun-Sil;Kim, Sam-Woong;Kang, Ho-Young;Bahk, Jeong-Dong
    • Journal of Life Science
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    • v.19 no.3
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    • pp.305-310
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    • 2009
  • Plasmid pGKV21 contains a 229-nucleotide (nt) single-strand DNA initiation (ssi) signal. Using asymmetric PCR, we prepared a small single-stranded (ss) DNA fragment of the ssi signal and, using the 229-nt ssDNA fragment, determined the requirements of RNA polymerase for priming and DNA-protein interaction. The ssi fragment prepared was able to generate primer RNAs with almost the same efficiency as the $M13{\Delta}lac182/229$ phage DNA. However, the cssi (complementary strand of the ssi signal) fragment could not synthesize primer RNAs. This result suggests that the 229-nt ssi signal functions in a strand specific manner. Gel retardation and DNase I footprinting demonstrated that the synthesized ssi fragment could interact with both E. coli RNA polymerase and SSB protein to synthesize primer RNA. In Escherichia coli [pWVAp], an addition of rifampicin resulted in an accumulation of ssDNA, indicating that the host-encoded RNA polymerase is involved in the conversion of ssDNA to double-stranded plasmid DNA.

A Dynamic Channel Allocation Employing Smart Antenna to Resolve a Crossed Time-slot Problem in TD-SCDMA (TD-SCDMA에서 셀 간 교차 타임-슬롯 문제 해결을 위한 스마트 안테나 기반의 동적 채널 할당 방안)

  • Kim, Eun-Heon;Park, Jae-Hyun;Kim, Duk-Kyung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.32 no.12A
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    • pp.1276-1285
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    • 2007
  • Since the TD-SCDMA (Time Division-Synchronous Code Division Multiple Access) system is based on TDD (Time Division Duplexing), the uplink and downlink can be allocated asymmetrically according to the traffic e.g. Web browsing. Although this asymmetric allocation can increase the frequency utilization, it may cause time slot opposing, which implies the time slot is assigned in opposing direction between cells. The time slot opposing can generate significant interference between cells, which results in severe performance degradation. In the paper, a novel dynamic channel allocation (DCA) is proposed in the TD-SCDMA system, to mitigate the impact of time slot opposing considering smart antenna. When the smart antenna is applied in the system, the inter-cell interference is largely affected by beam pattern and beam direction between neighboring cells. Therefore, the time slot opposing and smart antenna should be considered together in the DCA. The intensive simulations show that the proposed scheme can improve the system capacity compared to the conventional DCA schemes.

Crystal Structures of the Two Isomorphous A-DNA Decamers d(GTACGCGTAC) and d(GGCCGCGGCC)

  • Kim, Tae-gyun;Kwon, Taek-Hun;Jung, Hye-sun;Ku, Ja-Kang;Sundaralingam, Muttaiya;Ban, Chang-ill
    • Bulletin of the Korean Chemical Society
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    • v.27 no.4
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    • pp.568-572
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    • 2006
  • To study the effect of sequence on DNA structure, the two decamer crystal structures one alternating,d(GTACGCGTAC), and the other non-alternating, d(GGCCGCGGCC), were solved. Crystals of both decamers belong to the hexagonal space group $P6_122$, with one strand in the asymmetric unit. The unit cell constants of the alternating decamer are a = b = 39.26 $\AA$, c = 77.70 $\AA$. The structure was refined with 1,828 reflections from 8.0 to 2.0 Aresolution to an R value of 21.3% with all DNA atoms and 63 water molecules. The isomorphous non-alternating decamer had unit cell dimensions of a = b = 39.05 $\AA$, c = 82.15 $\AA$. The structure was refined with 2,423 reflections from 8.0 to 2.0 $\AA$ resolution to a final R value of 22.2% for all DNA atoms and 65 water molecules. Although the average helical parameters of the decamers are typical of A-DNAs, there are some minor differences between them. The helical twist, rise, x-displacement, inclination and roll alternate in the alternating decamer, but do not in the non-alternating decamer. The backbone conformations in both structures show some differences; the residue G(7) of the alternating decamer is trans for $\alpha$ and $\gamma$ while the trans conformations are observed at the residue G(8) of the non-alternating decamer.

A study on the brownish ring of quartz glass crucible for silicon single crystal ingot (실리콘 단결정 잉곳용 석영유리 도가니의 brownish ring에 대한 연구)

  • Jung, YoonSung;Choi, Jae Ho;Min, Kyung Won;Byun, Young Min;Im, Won Bin;Noh, Sung-Hun;Kang, Nam-Hun;Kim, Hyeong-Jun
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.32 no.3
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    • pp.115-120
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    • 2022
  • A brown ring (hereinafter referred to as BR) on the inner surface of a quartz glass crucible used in the manufacturing process of a silicon ingot for semiconductor wafers was studied. BR is 20~30 ㎛ in size and has an asymmetric brown ring shape. The size and distribution of BR were different depending on the crucible location, and the size and distribution of BR were the largest and most abundant in the round part with the highest crucible temperature during Si ingot growth. BR contains cristobalite, which has a higher coefficient of thermal expansion than quartz glass, so it is considered that surface cracks appear. The color development of BR and pin holes are presumed to be due to oxygen vacancies.

Cloning and Characterization of Porcine Uroplakin II Gene

  • D. N. Kwon;H. K. Shin;C. K. Hwang;D. W. Ok;Kim, J. H.
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.19-19
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    • 2001
  • Mammalian urothelium undergoes unique membrane specialization by making the asymmetric unit membrane (AUM) that is covered with the apical cell surface during terminal differentiation. The AUM contains several major integral membrane proteins including uroplakin Ia, Ib, II and III. The genes for uroplakins have been cloned from humans and mice, but not from porcine. In this study, we report the cloning of the UPII genomic DNA, which codes for the full length open reading frame for the uroplakin II protein. The deduced amino acid sequence encodes of a hydrophobic NH$_2$-terminal peptide, a prosequence, and a mature protein. The prosequence contains three potential N-glycosylation sites and a RGRR cleavage site that may be involved in uroplakin II processing and maturation. Northern and immunohistochemistry analyses showed that the porcine UPII gene is only expressed in urothelium and that the protein was specifically localized in urothelial superficial cells. A 2kb of upstream in the promoter sequence contains multiple transcription factor binding sites, including GC-box, SPI, AP2, and GATA-box sites, but not for TATA or CAAT-box sequences. Comparison of the porcine UPII promoter sequence with that of the murine by MEME system presented two conserved motifs, suggesting a cis-acting regulatory role for the conserved sequences. Sequence homology between two species in motif A and B was 79% and 80% respectively, although their relative locations were different. During the gestation, mouse bladder at estrus stages and day 10 after parturition showed higher UPII expression, while showed lower expression at peri-implantation stage. Taken together, our results showed that the porcine UPII gene was expressed highly and specifically in the bladder urothelium and that steroid hormones for implantation changed the expression of UPII in the bladder, although the biological significance of UPII remains to be not determined.

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