• Reproductive and Developmental Biology
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• v.36 no.1
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• pp.55-64
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• 2012

Peroxiredoxin II (Prdx II; a typical 2-Cys Prdx) has been originally isolated from erythrocytes, and its structure and peroxidase activity have been adequately studied. Prdx II has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in a variety of disease states associated with oxidative stress, including cancer and aging-associated pathologies. But, the precise mechanism is still obscure in various aspects of aging containing ovarian aging. Identification and relative quantification of the increased proteins affected by Prdx II deficiency may help identify novel signaling mechanisms that are important for oxidative stress-related diseases. To identify the increased proteins in Prdx $II^{-/-}$ mice, we performed RBC comparative proteome analysis in membrane fraction and cytosolic fractions by nano-UPLC-$MS^E$ shotgun proteomics. We found the increased 86 proteins in membrane (32 proteins) and cytosolic (54 proteins) fractions, and analyzed comparative expression pattern in healthy RBCs of Prdx $II^{+/+}$ mice, healthy RBCs of Prdx $II^{-/-}$ mice, and abnormal RBCs of Prdx $II^{-/-}$ mice. These proteins belonged to cellular functions related with RBC lifespan maintain, such as cellular morphology and assembly, cell-cell interaction, metabolism, and stress-induced signaling. Moreover, protein networks among the increased proteins were analyzed to associate with various diseases. Taken together, RBC proteome may provide clues to understand the clue about redox-imbalanced diseases.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.6
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• pp.1-6
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• 2002

Micro engine that includes Micro scale combustor is fabricated. Design target was focused on the observation of combustion driven actuation in MEMS scale. Combustor design parameters are somewhat less than the size recommended by feasibility test. The engine structure is fabricated by isotropic etching of the photosensitive glass wafers. Electrode is formed by electroplating of the Nickel. Photosensitive glass can be etched isotropically with almost vertical angle. Bonding and assembly of structured photosensitive glass wafer from the engine. Combustor size was determined to be 1mn scale. Piston in cylinder moves by fuel injection and reaction. In firing test, adequate engine operation including ignition, flame propagation and piston motion was observed. Present study warrants further application research on MEMS scale internal combustion power units.

• Design & Manufacturing
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• v.14 no.3
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• pp.23-30
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• 2020

Laser Assisted Thermo-Compression Bonding (LATCB) has been proposed to improve the "chip tilt due to the difference in solder bump height" that occurs during the conventional semiconductor chip bonding process. The bonding module of the LATCB system has used a piezoelectric actuator to control the inclination of the compression jig on a micro scale, and the piezoelectric actuator has been directly coupled to the compression jig to minimize the assembly tolerance of the compression jig. However, this structure generates a lateral force in the piezoelectric actuator when the compression jig is tilted, and the stacked piezoelectric element vulnerable to the lateral force has a risk of failure. In this paper, the optimal design of the flexure hinge was performed to minimize the lateral force generated in the piezoelectric actuator when the compression jig is tilted by using the displacement difference of the piezoelectric actuator in the bonding module for LATCB. The design variables of the flexure hinge were defined as the hinge height, the minimum diameter, and the notch radius. And the effect of the change of each variable on the stress generated in the flexible hinge and the lateral force acting on the piezoelectric actuator was analyzed. Also, optimization was carried out using commercial structural analysis software. As a result, when the displacement difference between the piezoelectric actuators is the maximum (90um), the maximum stress generated in the flexible hinge is 11.5% of the elastic limit of the hinge material, and the lateral force acting on the piezoelectric actuator is less than 1N.

• Journal of the Korean Society of Hazard Mitigation
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• v.6 no.4 s.23
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• pp.1-6
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• 2006

In terms of the speed-up of the conventional line, the research for a improved turnout was carried out continually around world. A turnout is known that it is the most unstable equipment structurally in the rail system. So, the turnout is needed more stable structure. An accurate assembly of turnout affects traveling qualify of turnout area and running safety. Therefore, a turnout which is pre-assembled at a factory by using the split PC sleeper is transported to construction site recently in advanced countries. In this paper, the performance measurements which estimate the safety of the split PC sleepers which are adopted for the first time to improve performance of turnout are implemented indoors. The split PC sleeper is one of components of the improved turnout. The test is considered of the worst ballast condition under the maintenance work as like STT(Swithc Tie Tamper) or MTT(Multiple Tie Tamper). These experiments results indicate that structural safety for the expansion joint of the split PC sleepers.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.40 no.5
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• pp.369-378
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• 2003

This paper presents design and VLSI implementations of a reassembly processor for ATM/AAL. The assembly processor is responsible for processing ATM cells from the receive physical interface. It controls the transfer of the AAL payload to host memory and performs all necessary SAR and CPCS checks. We propose the improved structure of cell identification algorithm and smart scatter method for host memory management. The proposed cell identification algorithm quickly locates the appropriate reassembly VC table based on the received VPI./VCI channel value in the ATM header. The cell identification algorithm also allow complete freedom in assignment of VCI/VPI values. The reassembly processor uses a smart scatter method to write cell payload data to host memory. It maintains the scatter operation and controls the incoming DMA block during scatter DMA to host memory. The proposed reassembly processor can perform reassembly checks on AAL. OAM cell. For an AAL5 connection, only CPCS checks, including the CRC32, are performed. In this paper, we proposed a practical reassembly architecture. The design of reassembly processor has become feasible using 0.6${\mu}{\textrm}{m}$ CMOS gate array technology.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2014.11a
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• pp.78-80
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• 2014

Silicone structural glazing (SSG) is a method utilizing a silicone adhesive to attach glass, metal, or other panel material to the structure of a building. Windload and other impact loads on the facade are transferred from the glass or panel through the silicone structural sealant to the systems' framework. Silicone structural glazing systems are currently a very common method of glazing throughout the world. Locally, structural silicone glazing has become very common to achieve aesthetically pleasing and high utilization of small land for both residential and commercial building. Although structural silicone glazing has been utilized for approximately thirty years in Korea, the understanding of its technology was low and limited. Consequently, Korean projects experienced many quality issues during assembly and construction, even in very recently finished buildings. Adhesion loss and water infiltration occurred on more than one project, and the time and cost to repair these issues were substantial. In general, there are two kinds of structural silicones depending on fabrication methods. 1part structural silicone is for site glazing system and 2part structural silicone is for unitized factory glazing system. In this paper, 2part structural silicone which is very common for factory fabricating curtainwall systems was evaluated with regards to various mixing ratio. Since the structural performance of 2part sealant can be affected by mixing ratios, some extra ranges of recommended mixing ratio were evaluated to see any performance differences. Besides on cure profile, comparative evaluations for mechanical properties and adhesion develop on common building substrates were conducted.

• Steel and Composite Structures
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• v.1 no.4
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• pp.427-440
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• 2001

The growing use of unprotected or partially protected steelwork in buildings has caused a lively debate regarding the safety of this form of construction. A good deal of recent research has indicated that steel members have a substantial inherent ability to resist fire so that additional fire protection can be either reduced or eliminated completely. A performance based philosophy also extends the study into the effect of structural continuity and the performance of the whole structural totality. As part of the structural system, thermal expansion during the heating phase or contraction during the cooling phase in most beams is likely to be restrained by adjacent parts of the whole system or sub-frame assembly due to compartmentation. This has not been properly addressed before. This paper describes an experimental programme in which unprotected steel beams were tested under load while it is restrained between two columns and additional horizontal restraints with particular concern on the effect of catenary action in the beams when subjected to large deflection at very high temperature. This paper also presents a three-dimensional mathematical modelling, based on the finite element method, of the series of fire tests on the part-frame. The complete analysis starts with an evaluation of temperature distribution in the structure at various time levels. It is followed by a detail 3-D finite element analysis on its structural response as a result of the changing temperature distribution. The principal part of the analysis makes use of an existing finite element package FEAST. The effect of columns being fire-protected and the beam being axially restrained has been modelled adequately in terms of their thermal and structural responses. The consequence of the beam being restrained is that the axial force in the restrained beam starts as a compression, which increases gradually up to a point when the material has deteriorated to such a level that the beam deflects excessively. The axial compression force drops rapidly and changes into a tension force leading to a catenary action, which slows down the beam deflection from running away. Design engineers will be benefited with the consideration of the catenary action.

• Journal of Magnetics
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• v.22 no.2
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• pp.299-305
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• 2017

Magnetic fluid, a new type of magnetic material, is a colloidal liquid constituted of nano-scale ferromagnetic particles suspended in carrier fluid. Magnetic fluid sealing is one of the most successful applications of magnetic fluid. As a new type of seal offering the advantages of no leakage, long life and high reliability, the magnetic fluid seal has been widely utilized under vacuum- and low-pressure-differential conditions. In practical applications, for improved pressure capacity, a multistage sealing structure is always used. However, in engineering applications, a uniform distribution of magnetic fluid under each tooth often cannot be achieved, which problem weakens the overall pressure capacity of the seals. In order to improve the pressure capacity of magnetic fluid seals and broaden their applications, the present study theoretically and experimentally analyzed the degree of non-uniform distribution of multistage magnetic fluid seals. A mathematical model reflecting the relationship between the pressure capacity and the distribution of magnetic fluid under a single tooth was constructed, and a formula showing the relationship between the volume of magnetic fluid and its contact width with the shaft was derived. Furthermore, the relationship of magnetic fluid volume to capacity was analyzed. Thereby, the causes of non-uniform distribution could be verified: injection of magnetic fluid; the assembly of magnetic fluid seals; the change of magnetic fluid silhouette under pressure loading; the magnetic fluid sealing mechanism of pressure transmission, and seal failure. In consideration of these causes, methods to improve the pressure capacity of magnetic fluid seals was devised (and is herein proposed).

• Journal of Korea Port Economic Association
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• v.26 no.4
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• pp.101-121
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• 2010

Domestic trucking transportation market does not have only the poor business environment but also the serious defects on the transportation trading structure. It makes weaken the competitiveness of trucking industry and hinders the development of trucking industry. The problems of trucking industry such as the recession of the Korean economy and decrease of the trucking charge cause the truck drivers suffer. Korean government and national assembly try to change the law of truck transportation business. The suggested law includes the obligation of trucking information networking system and it can make the transportation business structures more efficiently. This article deals with the strategies for the establishment of trucking transportation networking system and examines the cost-benefit analysis assuming the four demand senario. The results suggest the establishment of the information system not based on the individual trucking industry but on the joint industry at the level of the local area. This article also suggests that the information system has a positive financial feasibility.

• Bulletin of the Korean Chemical Society
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• v.34 no.2
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• pp.558-564
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• 2013

A simple and effective method is introduced to synthesize a series of polystyrene-b-poly(oligo(ethylene oxide) monomethyl ether methacrylate)-b-polystyrene (PSt-b-POEOMA-b-PSt) triblock copolymers. The structures of PSt-b-POEOMA-b-PSt copolymers were characterized by Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance ($^1H$ NMR) spectroscopy. The molecular weight and molecular weight distribution of the copolymer were measured by gel permeation chromatography (GPC). Furthermore, the self-assembling and drug-loaded behaviours of three different ratios of PSt-b-POEOMA-b-PSt were studied. These copolymers could readily self-assemble into micelles in aqueous solution. The vitamin E-loaded copolymer micelles were produced by the dialysis method. The micelle size and core-shell structure of the block copolymer micelles and the drug-loaded micelles were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The thermal properties of the copolymer micelles before and after drug-loaded were investigated by different scanning calorimetry (DSC). The results show that the micelle size is slightly increased with increasing the content of hydrophobic segments and the micelles are still core-shell spherical structures after drug-loaded. Moreover, the glass transition temperature (Tg) of polystyrene is reduced after the drug loaded. The drug loading content (DLC) of the copolymer micelles is 70%-80% by ultraviolet (UV) photolithography analysis. These properties indicate the micelles self-assembled from PSt-b-POEOMA-b-PSt copolymers would have potential as carriers for the encapsulation of hydrophobic drugs.