• Asian-Australasian Journal of Animal Sciences
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• v.22 no.11
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• pp.1555-1565
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• 2009

Anaerobic rumen fungi have been regarded as good genetic resources for enzyme production which might be useful for feed supplements, bio-energy production, bio-remediation and other industrial purposes. In this study, an expressed sequence tag (EST) library of the rumen anaerobic fungus Neocallimastix frontalis was constructed and functional genes from the EST library were analyzed to elucidate carbohydrate metabolism of anaerobic fungi. From 10,080 acquired clones, 9,569 clones with average size of 628 bp were selected for analysis. After the assembling process, 1,410 contigs were assembled and 1,369 sequences remained as singletons. 1,192 sequences were matched with proteins in the public data base with known function and 693 of them were matched with proteins isolated from fungi. One hundred and fifty four sequences were classified as genes related with biological process and 328 sequences were classified as genes related with cellular components. Most of the enzymes in the pathway of glucose metabolism were successfully isolated via construction of 10,080 ESTs. Four kinds of hemi-cellulase were isolated such as mannanase, xylose isomerase, xylan esterase, and xylanase. Five $\beta$-glucosidases with at least three different conserved domain structures were isolated. Ten cellulases with at least five different conserved domain structures were isolated. This is the first solid data supporting the expression of a multiple enzyme system in the fungus N. frontalis for polysaccharide hydrolysis.

• Journal of Korea Port Economic Association
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• v.24 no.1
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• pp.23-39
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• 2008

This paper outlines a plan to promote South Jeonnam region's logistics capability in connection with the regional logistics bases such as Gwangyang Port, Jangseong Integrated Freight Terminal, Mokpo Port and Muan International Airport. The capability of regional innovation bodies, and industrial innovation generally, needs to be improved by establishing a systematic regional innovation plan. Smaller import and export logistics bases, presently scattered in downtown Gwangju and its suburban area, need to be integrated as soon as possible, in advance of the completion of a traffic network connecting all of the triangle logistics bases. Moreover, plans to develop Muan International Airport to the status of a hub airport need to be established, and its function as an air freight terminal should be strengthened. Gwangyang Port and Mokpo Port should be developed as Korea's key harbors with international logistics bases the two ports, presently conducting simple cargo work, should be transformed into general logistics ports conducting logistics, assembling, trading, and international business by developing their surrounding areas.

• Journal of the Microelectronics and Packaging Society
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• v.22 no.2
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• pp.11-19
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• 2015

Since CMOS device scaling has stalled, three-dimensional (3-D) integration allows extending Moore's law to ever high density, higher functionality, higher performance, and more diversed materials and devices to be integrated with lower cost. 3-D integration has many benefits such as increased multi-functionality, increased performance, increased data bandwidth, reduced power, small form factor, reduced packaging volume, because it vertically stacks multiple materials, technologies, and functional components such as processor, memory, sensors, logic, analog, and power ICs into one stacked chip. Anticipated applications start with memory, handheld devices, and high-performance computers and especially extend to multifunctional convengence systems such as cloud networking for internet of things, exascale computing for big data server, electrical vehicle system for future automotive, radioactivity safety system, energy harvesting system and, wireless implantable medical system by flexible heterogeneous integrations involving CMOS, MEMS, sensors and photonic circuits. However, heterogeneous integration of different functional devices has many technical challenges owing to various types of size, thickness, and substrate of different functional devices, because they were fabricated by different technologies. This paper describes new 3-D heterogeneous integration technologies of chip self-assembling stacking and 3-D heterogeneous opto-electronics integration, backside TSV fabrication developed by Tohoku University for multifunctional convergence systems. The paper introduce a high speed sensing, highly parallel processing image sensor system comprising a 3-D stacked image sensor with extremely fast signal sensing and processing speed and a 3-D stacked microprocessor with a self-test and self-repair function for autonomous driving assist fabricated by 3-D heterogeneous integration technologies.

• Journal of the Korean Institute of Rural Architecture
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• v.18 no.1
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• pp.19-28
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• 2016

This research aimed to suggest a standard design that reflects Hanok design tendency, and present a selective design that can fulfill a building owner's intention beyond the simple function of building permit or report. In addition, this research attempted to become a standard by establishing a criterion in calculating the measurements of section design or primary framework members, in order to be a guideline for designing Hanok in different sizes and forms. The results are as follows. The building area of Hanok standard design was set to be below $85m^2$, with a straight type of $83.16m^2$ and an L-shape of $84.24m^2$. By dividing the plane into a straight type and L-shape, two straight types were suggested: 'general type' and 'large living room type.' The upper floor space, along with the main room and small room, was proposed as an option to be changed into a room where an underfloor heating is installed depending on the building owner's intention. In addition, a criterion for side design and calculation of framework measurements was suggested and applied, while a five-girder design without high pillars was suggested for material-assembling structure. Two types of pillars-circumference and square cylinder-were proposed for the building owner to choose from, and a pointed beam house and ikgong(orthogonally-projected bracket) house were suggested for pojak bracket structure so either of them could be chosen according to the building owner's taste and economic condition. Finally, the sectional size of main materials were divided according to the form of pojak bracket structure to be proposed.

• The KIPS Transactions:PartD
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• v.10D no.2
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• pp.261-266
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• 2003

A protein, which is a linear polymer of amino acids, is one of the most important bio-molecules composing biological structures and regulating bio-chemical reactions. Since the characteristics and functions of proteins are determined by their amino acid sequences in principle, protein sequence determination is the starting point of protein function study. This paper proposes a protein sequence prediction method based on data mining techniques, which can overcome the limitation of previous bio-chemical sequencing methods. After applying multiple proteases to acquire overlapped protein fragments, we can identify candidate fragment sequences by comparing fragment mass values with peptide databases. We propose a method to construct multi-partite graph and search maximal paths to determine the protein sequence by assembling proper candidate sequences. In addition, experimental results based on the SWISS-PROT database showing the validity of the proposed method is presented.

• Journal of KIISE:Software and Applications
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• v.32 no.8
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• pp.704-716
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• 2005

Assembling new software systems from Prepared components is an attractive alternative to traditional software development method to reduce development cost and schedule dramatically. However, if separately developed components are tested, integrated and verified with unreasonable effort and high cost, it would not be an effective way to software development. Components are not distributed in the shape of white-box source code so that should be hard to validate and test in new application environment. For solving this problem, built-in tester components are suggested to check the contract-compliance of their server components. If components have various and complex function, built-in tester should be heavy and unflexible to test in composition of components. This paper suggests enhancing automated wrapper technique which substitutes with built-in tester components and shows the usability of the wrapper by design and implementation. Component testing in this way reduces the cost and effort associated with preparation of component testing and makes the various test experiments in components assembly.

• Proceeding of Spring/Autumn Annual Conference of KHA
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• 2008.04a
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• pp.421-426
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• 2008

In case of the large-scaled indoor gymnasium that has been constructed in the local area, there are many instances for the use as multipurpose space where the public performances are possible such as leisure activity, lecture, assembling activity, drama, concert and so on for the resident together with the purpose of sporting facility. In order for utilization to the maximum of the function of such indoor gymnasium, the acoustic capabilities concerned with Definition of both Voice and Music are simultaneously required. However, in case of the large-scaled athletic facility, since it was designed with high ceiling-height in view of its characteristics, it forms a Sound Focus and then the sound is concentrating to the specific part, and because the vibration of sound is too loud due to its broad volume, the acoustic defects such as many Echoes are arising. On such viewpoint, based on the drawing of the indoor gymnasium that is scheduled to be built at B County, Chonbuk Province, this Study has proposed such indoor gymnasium equipped with the optimized acoustic condition passing through Acoustic Simulation Phase. As the result of Acoustic Simulation, we could design the indoor gymnasium that equipped with really satisfying acoustic performance 'after' reformation compared with 'before' reformation, and it is considering that such material could be utilized as the fundamental material that brings a curtailment effect of the construction cost and also enables us to improve the acoustic performance, at the stage of planning and designing for the similar sporting facility in the future.

• BMB Reports
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• v.35 no.2
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• pp.186-193
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• 2002

The acid-bible subunit (ALS) associates with the insulinlike growth factor (IGF)-I or II, and the IGF binding protein-3 (IGFBP-3) in order to form a 150-kD complex in the circulation. This complex may regulate the serum IGFs by restricting them in the vascular system and promoting their endocrine actions. Little is known about how ALS binds to IGFBP3, which connects the IGFs to ALS. Xenopus oocyte was utilized to study the function of ALS in assembling IGFs into the ternary complexes. Xenopus oocyte was shown to correctly translate in vitro transcribed mRNAs of ALS and IGFBP3. IGFBP3 and ALS mRNAs were injected in a mixture, and their products were immunoprecipitated by antisera against ALS and IGFBP3. Contrary to traditional reports that ALS interacts only with IGF-bound IGFBP3, this study shows that ALS is capable of forming a binary complex with IGFBP3 in the absence of IGF When cross-linked by disuccinimidyl suberate, the band that represents the ALS-IGFBP3 complex was evident on the PAGE. IGFBP3 movement was monitored according to the distribution between the hemispheres. Following a localized translation in the vegetal hemisphere, IGFBP3 remained in the vegetal half in the presence of ALS. However, the mutant IGFBP3 freely diffused into the animal half, despite the presence of ALS, which is different from the wild type IGFBP3. This study, therefore, suggests that ALS may play an important role in sequestering IGFBP3 polypeptides via the intermolecular aggregation. Studies using this heterologous model will lead to a better understanding of the IGFBP3 and ALS that assemble into the ternary structure and circulate the IGF system.

• Corrosion Science and Technology
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• v.11 no.2
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• pp.65-69
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• 2012

One dimensional(1D) $Na_2Ti_6O_{13}$ nanorods with 70 nm in diameter was synthesized by a molten salt method. Using the synthesized nanorods, about 750 nm thick $Na_2Ti_6O_{13}$ film was coated on Fluorine-doped tin oxide(FTO) glasss substrate by the Layer-by-layer self-assembly(LBL-SA) method in which a repetitive self-assembling of ions containing an opposite electric charge in an aqueous solution was utilized. Using the Kubelka-Munk function, the band gap energy of the 1D-$Na_2Ti_6O_{13}$ nanorods was nalyzed to be 3.5 eV. On the other hand, the band gap energy of the $Na_2Ti_6O_{13}$ film coated on FTO was found to be a reduced value of 2.9 eV, resulting from the nano-scale and high porosity of the film processed by LBL-SA method, which was favorable for the photo absorption capability. A significant improvement of photocurrent and onset voltage was observed with the $Na_2Ti_6O_{13}$ film incorporated into the conventional $Fe_2O_3$ photoelectrode: the photocurrent increased from 0.25 to 0.82 mA/$cm^2$, the onset voltage decreased from 0.95 to 0.78 V.

• Korean Institute of Interior Design Journal
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• v.24 no.5
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• pp.62-69
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• 2015

The purpose of this research is to analyze the methods of the decorations using module plants in interior spaces. This research produced 18 types of the module plant decoration: considering the classifications of module plants(soil, hydroculture, moss), directions of module plants (up, side, down), assembling ways of module plants (horizontal, vertical). Applying these 18 types to the interior space decoration (floor stand, wall attach, ceiling hanging), 54 types were classified. After that, 150 cases of the decoration using module plants in interior spaces were collected and analyzed. In result, the cases were belong to 25 types of 54 types. The important types were the types to be able to decorate wide area of walls or ceilings without occupying floor area: SOIL-UP-VERTICAL, HYDROCULTURE-UP-VERTICAL, MOSS-SIDE-VERTICAL. These types were the decorations with function of bio-filter for air cleaning. Special types were SOIL-SIDE-HORIZONTAL, SOIL-SIDE-VERTICAL with soil developed not to pour and SOIL-DOWN-HORIZONTAL, SOIL-DOWN-VERTICAL with lucks not to pour soil. Plants will be used widely in interior design because of the awareness of eco-friendly design. The strength that module plants are portable, changable, able to exchange parts helps users to maintain plants in interior spaces. For designers, module plants are flexible materials in order to make variety of forms to adjust to interior spaces. The results of this research about methods of the decorations using module plants in interior spaces are useful to designers who want to design interior spaces eco-friendly and user-friendly.