• Title/Summary/Keyword: Aspect dependence

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Myofibrillogenesis in Embryonic Chick Pectoral Muscle Cultures: An Immunoelectron Microscopic Study (계배 배양 출근세포에 있어서의 근원섬유 형성과정 :면역 전자현미경적 연구)

  • 하재청;김한도김동수
    • The Korean Journal of Zoology
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    • v.35 no.2
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    • pp.149-160
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    • 1992
  • Using the protein A-gold complex, the mvoabrillogenesis and actin localization of cultured myoblast were invastisated. In the superstructural changes of mvogenic cell during differentiation, pectoral myoblasts contained large nucleus and numerous ribosomes but no myofibrils during the first 24 hr of cultures. Mvoblast initiated to differentiate at 3-day of culture contained the primitive myofibrillar structure. At 96 hr of culture, the mvofibrillar structure showed reletively discernable Z band but pools defined A, H and M bands. The feature of sarcomeric structure showed more defined form at cultur 5 day. In the aspect of actin localization, actin wvas diffusely detected throughout the cytoplasm of myogenic cell and nucleus during the proliferating stage. At 72 hr of culture, with the appearantc oi primitive mvofibrils, gold particles were observed in surrounding of myofibrils but still presented in overall of cytoplasm, especially in the surface and lumen of endoplasmic reticulum. With the gradual increase of culture time, local distribution of actin was readily detected within cytoplasm. In the 5-day specimen of cultures, gold particles precisely indicate the sites of actin localifation within the sarcomere. These results indicate the time of onset of myofibrill appearance and the biosynthetic and incorporation pathway of actin molecules into sarcomeric structure during myofibrillogenesis. Thus, in the present study, the first mvoabrillar structure was detected at culture 3 day, and the initiation of assembly into a typical sarcmeric structure was observed at culture 5 day. It seems, however, that the course of events on myofibrillogenesis of cultured myoblasts can be changed with great dependence of culture conditions including the number and groluth rate of mononucleated mvoblasts after seeding although the fundamental process shows identical appearances.

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Microstructure and Giant Magnetoresistance of AgCo Nano-granular Alloy Films (Ag-Co합금박막의 두께에 따르는 미세구조 변화 및 자기저항 거동)

  • 이성래;김세휘
    • Journal of the Korean Magnetics Society
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    • v.8 no.3
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    • pp.131-137
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    • 1998
  • The thickness dependence of the microstructure and the giant magnetoresistance behavior of co-evaporated Co-Ag granular alloy films were investigated. The maximum magnetoresistance ratio of 24% was observed in the the as-deposited state of the 40 at. % Co alloy having 200 nm thickness. The surface scattering contributed about 20% to the total resistivity in the 20 nm thick films. The MR ratio dropped sharply when the film thickness was below 50 nm. The reduction in the Co particle size and the increase in solid solubility of Ag in fcc Co when the film thickness decreased were observed using a high resolution TEM. The aspect ratio of the Co particles was also affected by the film thickness. Those microstructural changes as well as the surface induced spin flipping play a significant role in the $\Delta$p change.

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Characteristics of single/poly crystalline silicon etching by$Ar^+$ ion laser for MEMS applications (MEMS 응용을 위한 $Ar^+$ 이온 레이저에 의한 단결정/다결정 실리콘 식각 특성)

  • Lee, Hyun-Ki;Han, Seung-Oh;Park, Jung-Ho;Lee, Cheon
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.48 no.5
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    • pp.396-401
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    • 1999
  • In this study, $Ar^+$ ion laser etching process of single/poly-crystalline Si with $CCl_2F_2$ gas is investigated for MEMS applications. In general, laser direct etching process is useful in microelectronic process, fabrication of micro sensors and actuators, rapid prototyping, and complementary processing because of the advantages of 3D micromachining, local etching/deposition process, and maskless process with high resolution. In this study, a pyrolytic method, in which $CCl_2F_2$ gasetches molten Si by the focused laser, was used. In order to analyze the temperature profile of Si by the focused laser, the 3D heat conduction equation was analytically solved. In order to investigate the process parameters dependence of etching characteristics, laser power, $CCl_2F_2$ gas pressure, and scanning speed were varied and the experimental results were observed by SEM. The aspect ratio was measured in multiple scanning and the simple 3D structure was fabricated. In addition, the etching characteristics of $6\mum$ thick poly-crystalline Si on the insulator was investigated to obtain flat bottom and vertical side wall for MEMS applications.

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Reliability Improvement of Offshore Structural Steel F690 Using Surface Crack Nondamaging Technology

  • Lee, Weon-Gu;Gu, Kyoung-Hee;Kim, Cheol-Su;Nam, Ki-Woo
    • Journal of Ocean Engineering and Technology
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    • v.35 no.5
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    • pp.327-335
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    • 2021
  • Microcracks can rapidly grow and develop in high-strength steels used in offshore structures. It is important to render these microcracks harmless to ensure the safety and reliability of offshore structures. Here, the dependence of the aspect ratio (As) of the maximum depth of harmless crack (ahlm) was evaluated under three different conditions considering the threshold stress intensity factor (Δkth) and residual stress of offshore structural steel F690. The threshold stress intensity factor and fatigue limit of fatigue crack propagation, dependent on crack dimensions, were evaluated using Ando's equation, which considers the plastic behavior of fatigue and the stress ratio. ahlm by peening was analyzed using the relationship between Δkth obtained by Ando's equation and Δkth obtained by the sum of applied stress and residual stress. The plate specimen had a width 2W = 12 mm and thickness t = 20 mm, and four value of As were considered: 1.0, 0.6, 0.3, and 0.1. The ahlm was larger as the compressive residual stress distribution increased. Additionally, an increase in the values of As and Δkth(l) led to a larger ahlm. With a safety factor (N) of 2.0, the long-term safety and reliability of structures constructed using F690 can be secured with needle peening. It is necessary to apply a more sensitive non-destructive inspection technique as a non-destructive inspection method for crack detection could not be used to observe fatigue cracks that reduced the fatigue limit of smooth specimens by 50% in the three types of residual stresses considered. The usefulness of non-destructive inspection and non-damaging techniques was reviewed based on the relationship between ahlm, aNDI (minimum crack depth detectable in non-destructive inspection), acr N (crack depth that reduces the fatigue limit to 1/N), and As.

Smart Structural Health Monitoring Using Carbon Nanotube Polymer Composites (탄소나노튜브 고분자 복합체 기반 스마트 구조건전성 진단)

  • Park, Young-Bin;Pham, Giang T.;Wang, Ben;Kim, Sang-Woo
    • Composites Research
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    • v.22 no.6
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    • pp.1-6
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    • 2009
  • This paper presents an experimental study on the piezoresistive behavior of nanocomposite strain sensors subjected to various loading modes and their capability to detect structural deformations and damages. The electrically conductive nanocomposites were fabricated in the form of a film using various types of thermoplastic polymers and multi-walled carbon nanotubes (MWNTs) at various loadings. In this study, the nanocomposite strain sensors were bonded to a substrate and subjected to tension, flexure, or compression. In tension and flexure, the resistivity change showed dependence on measurement direction, indicating that the sensors can be used for multi-directional strain sensing. In addition, the sensors exhibited a decreasing behavior in resistivity as the compressive load was applied, suggesting that they can be used for pressure sensing. This study demonstrates that the nanocomposite strain sensors can provide a pathway to affordable, effective, and versatile structural health monitoring.

Effects of oscillation parameters on aerodynamic behavior of a rectangular 5:1 cylinder near resonance frequency

  • Pengcheng Zou;Shuyang Cao;Jinxin Cao
    • Wind and Structures
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    • v.38 no.1
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    • pp.59-74
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    • 2024
  • Large Eddy Simulation (LES) is used to explore the influence of vibration frequency and amplitude on the aerodynamic performance of a rectangular cylinder with an aspect ratio of B/D=5 (B: breadth; D: depth of cylinder) at a Reynolds number of 22,000 near resonance frequency. In smooth flow conditions, the research employs a sequence of three-dimensional simulations under forced vibration with diverse frequency ratios fe / fo = 0.8-1.2 (fe : oscillation frequency; fo : Strouhal frequency when the rectangular cylinder is stationary ) and oscillation amplitudes Ah/D = 0.05 - 0.3. The individual influences of fe / fo and Ah/D on the characteristics of integrated and distributed aerodynamic forces are the focal points of discussion. For the integrated aerodynamic force, particular emphasis is placed on the analysis of the dependence of velocity-proportional component C1 and displacement-proportional component C2 of unsteady aerodynamic force on amplitude and frequency ratio. Near the resonance frequency, the dependencies of C1 and C2 on amplitude are stronger than that of frequency ratio. For the distributed aerodynamic force, the increase in frequency and amplitude promotes the position of the main vortex core and reattachment to the leading edge in the streamwise direction. In the spanwise direction, vibration enhances the spanwise correlation of aerodynamic force to weaken the three-dimensional effect of the flow field, and a lower frequency ratio and larger amplitude amplify this effect.

A Study on the Axial Dependence of the Traffic Distribution Function (통항분포함수 축방향 의존성에 관한 연구)

  • Yoo, Sang-Lok;Gang, Sang-Geun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.21 no.2
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    • pp.179-187
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    • 2015
  • The purpose of this study is to identify the aspect that the traffic distribution function changes, according to the direction of the datum line and the horizontal and vertical positions of the datum point applied when it is calculated. Targeting routes at the entrance of Mokpo Harbor, this study tested using AIS survey data of January 2013 the effects of the three variables-direction of the datum line(${\theta}$), horizontal position($\mathfrak{L}_H$) and vertical position($\mathfrak{L}_V$) on mean ($\bar{x}$) and standard deviation (${\delta}$). The test result showed that $\bar{x}$ and ${\delta}$ were changed according to the change of ${\theta}$, because the extracted sample data were changed according to ${\theta}$; and the changes of $\bar{x}$ and ${\delta}$ according to ${\theta}$ were drawn as the relation of the sine function' sum. In addition, it was found that setting up ${\theta}$ that the change value of ${\delta}$ becomes the least as the direction of the datum line was valid, to determine the optimum passage distribution function on complex waters with multiple branches of route. The result of this study is expected to be used as basic data to understand maritime traffic flow based on more quantified data of normal distribution and make decisions related to maritime traffic safety management.

A Study on the Collaboration Types of Knowledge Creation in Korean Government-supported Research Institutes of S&T: Focusing on Openness and Diversity (과학기술계 출연연구기관의 지식창출 협력유형 분석에 관한 연구: 개방성과 다양성 중심으로)

  • Park, Joohyoung;Lee, Heesang
    • Journal of Korea Technology Innovation Society
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    • v.17 no.3
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    • pp.563-583
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    • 2014
  • We classified collaboration types of Government-supported Research Institutes (GRIs) in Korea to make a creation knowledge with the openness and the diversity. The openness is described to collaboration dependence and leading intensity, and the diversity is expressed to collaboration diversity and collaboration concentration. We suggest four taxonomy models explained in terms of the openness and the diversity with country level and institute level. This study uses 39,507 papers in SCOUPS DB that include author(s) affiliated to 26 GRIs. It concludes that many GRIs is classified to the type of multilateral collaboration follower in institute level and to the type of global network player in country level from the perspective of openness. In the aspect of diversity, many GRIs is concentrated to the collaboration type of picky eater or monophagous in institute level and to the multinatioal asymmetry type or non-multinational asymmetry type in country level. It is necessary for GRIs to lead collaboration and to transfer inhouse R&D institutes with high performance to be competitive institutes which make high quality knowledge.

A Study on Field Applicability of Underground Electric Heating Mesh (매설용 전기 발열 매시의 융설 효과에 대한 현장 적용성 연구)

  • Suh, Young-Chan;Seo, Byung-Seok;Song, Jung-Kon;Cho, Nam-Hyun
    • International Journal of Highway Engineering
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    • v.15 no.2
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    • pp.19-27
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    • 2013
  • PURPOSES : This study aims to investigate the snow-melt effects of an underground electric heater's snow-melt system via a field performance test, for evaluating the suitability of the system for use on a concrete pavement. The study also investigates the effectiveness of dynamic measures for clearing snow after snowfall events. METHODS : In order to check the field applicability, in November 2010, specimens were prepared from materials used for constructing concrete pavements, and underground electric heating meshes (HOT-mesh) were buried at depths of 50 mm and 100 mm at the site of the Incheon International Airport Construction Research Institute. Further, an automatic heating control system, including a motion sensor and pavement-temperature-controlled sensor, were installed at the site; the former sensor was intended for determining snow-melt effects of the heating control system for different snowfall intensities. Pavement snow-melt effects on snowy days from December 2010 to January 2011 were examined by managing the electric heating meshes and the heating control system. In addition, data on pavement temperature changes resulting from the use of the heating meshes and heating control system and on the dependence of the correlation between the outdoor air temperature and the time taken for the required temperature rise on the depth of the heating meshes were collected and analyzed. RESULTS : The effects of the heating control system's preheat temperature and the hot meshes buried at depths of 50 mm and 100 mm on the melting of snow for snowfalls of different intensities have been verified. From the study of the time taken for the specimen's surface temperature to increase from the preheat temperature ($0^{\circ}C$) to the reference temperature ($5{\sim}8^{\circ}C$) for different snowfall intensities, the correlation between the burial depth and outdoor air temperature has been determined to be as follows: Time=15.10+1.141Depth-6.465Temp CONCLUSIONS : The following measures are suggested. For the effective use of the electric heating mesh, it should be located under a slab it may be put to practical use by positioning it under a slab. From the management aspect, the heating control system should be adjusted according to weather conditions, that is, the snowfall intensity.

A Short Reveiw on the Acupoints Used in Cocaine Studies (코카인 연구에 사용된 경혈에 대한 소고(小考))

  • Lee, Bong Hyo;Lim, Sung Chul;Kim, Jae Soo;Lee, Yun Kyu;Lee, Hyun Jong;Jung, Tae Young;Lee, Sang Nam
    • Korean Journal of Acupuncture
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    • v.30 no.3
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    • pp.145-150
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    • 2013
  • Objectives : The aim of this study is to review the acupoints used in cocaine studies and interpret the effectiveness in an aspect of meridian theory of Korean medicine. Methods : Authors searched 50 articles in PubMed, 16 in KISS, 9 in OASIS, 40 in NDSL, and 19 in DBPIA, with the keyword of 'cocaine' or 'acupuncture'. The articles written in English only were included. The articles related with cocaine(abuse, dependence, sensitization, intake, withdrawal sign, withdrawal syndrome, reinstatement, seeking) only were subjected. The articles which used manual- or electro-acupuncture were included, and auricular was excluded. Results : The most frequently used acupoint was HT7, while LI4, LU9, ST36, and SP6 were used once respectively. HT7 could be understood to be used from the meaning of the name as well as the theory that Heart Meridian is deeply related with the brain function. LU9 seems to be used to enhance the effect of HT7. LI4 is thought to control reverting yin that flows toward the top of head following the theory of Jang Bu Sang Tong. ST36 appears to be used because it could regulate stress reaction that reverting yin is associated with. SP6 seems to have effect on brain function due to its ability to control spiritual disorder. Conclusions : The acupoints used in the cocaine studies are thought to influence the brain function through diverse mechanism, and Jang Bu Sang Tong theory seems to be involved in the mechanism, at least in part.