• Title/Summary/Keyword: Aspartic Acid

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Identification of Amino Acid Residues Involved in Xylanase Activity from Bacillus Pumilus TX703 (Bacillus Pumilus TX703 유래 Xylanase의 활성에 관여하는 아미노산 잔기의 확인)

  • Park Young-Seo
    • Journal of Life Science
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    • v.15 no.4 s.71
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    • pp.633-640
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    • 2005
  • The purified xylanase from Bacillus pumilus TX703 was modified with various chemical modifiers to determine the active sites of the enzyme. Treatment of the enzyme with group-specific reagents such as carbodiimide or N-bromosuccinimide resulted in complete loss of enzyme activity. These results assumed that these reagents reacted with glutamic acid or aspartic acid and tryptophan residues located at or near the active site. In each case, inactivation was performed by pseudo first-order kinetics. Inhibition of enzyme activity by carbodiimide and W-bromosuccinimide showed non-competitive and competitive inhibition type, respectively. Addition of xylan to the enzyme solution containing N-bromosuccinimide prevented the inactivation, indicating the presence of tryptophan at the substrate binding site. Analysis of kinetics for inactivation showed that the loss of enzyme activity was due to modification of two glutamic acid or aspartic acid residues and single tryptophan residue.

A Study on the Free Sugar and Amino Acids in Korean Arrowroot (칡뿌리의 유리당 및 아미노산 조성에 관한 연구)

  • 신수철
    • Korean Journal of Plant Resources
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    • v.10 no.1
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    • pp.1-5
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    • 1997
  • Proximate composition and the contents of free sugar and amino acid of Korean arrowroot were invesigated to develop and produce the high quality processed products. A higher content of moisture, protein, and fiber were found in male arrowroot than in those of female. Among the total free sugars, sucrose was the highest components and fructose and glucose were follwed. In Korean arrowroot, 17 amino acids were determined and proline of male arrowroot and aspartic acid of female arrowroot were found to be the major components. Arginine was abundant in male arrowroot, while aspartic acid was abundant in female arrowroot on the samples of November. Leucine was abundant in male arrowroot and glutamic acid was in female arrowroot in the samples of February. Among the free amino acid of Korean arrowroots, proline was the most plentiful and major components were histidine, alanine and serine.

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Free amino acids of brown rice in relation to specific gravity grade (비중선별(比重選別) 현미중(玄米中) 유리 Amino산 함량)

  • Park, H.;Chun, J.K.;Cho, I.H.
    • Applied Biological Chemistry
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    • v.15 no.1
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    • pp.35-40
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    • 1972
  • The contents of free amino acids in deembryod brown rice of two varieties were investigated by amino acid autoanalizer in relation to specific gravity grade. The analytical methods of free amino acid were also discussed. 1) The lower the specific gravity of the unhulled rice the higher the content of total free amino acids in the deembryod brown rice, and the similar trend appears to hold on each amino acids. 2) Main free amino acids were serine+asparagine, glutamic acid, aspartic acid, alanine and valine, and maximum values of them were 7.3, 5.1, 4.0, 3.4, 0.9mg/100g rice, respectively. They consist about 85% of total free amino acids in most cases. 3) The contents of soluble nitrogen and free amino acids appear to be lower in high protein variety (IR 667) than in low protein variety (Jinhung). The percentage of free amino acid nitrogen to soluble nitrogen, however, appears to be higher in high protein variety (IR 667). 4) Alanine was much lower than aspartic acid in IR 667 having Indica blood while alanine appears to be higher than aspartic acid in Jinhung (Japonica rice) suggesting varietal difference in amino acid metabolism. 5) Threonine peak was overlaped with glutamine, and serine was with asparagine in this study.

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Composition in Amino Acid and Changes in Protein Mineral Contents during Storage of Black Goat Extracts (흑염소 증탕액의 아미노산 조성 및 저장 중 단백질과 무기물의 변화)

  • 박창일;김영직
    • Food Science of Animal Resources
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    • v.20 no.4
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    • pp.257-263
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    • 2000
  • 본 연구는 체중 18~19kg(16~17개월령)의 측염소 3 두(♀)를 반도체로 한 후 흑염소육에 한약재를 첨가한 것을 가약 흑염소 소주로 하고, 흑염소육만 증탕한 것을 순 흑염소 소주로 하였고, 한약재만 증탕한 것을 한약재 증탕액으로 하여 4$^{\circ}C$에 30일간 저장하면서 VBN, 아미노산 무기질 조성 변화를 분석하여 다음과 같은 결과를 얻었다. VBN함량은 모든 처리구에서 저장기간이 경과하면서 증가하는 경향이었고, 가약 흑염소 소주보다 순 흑염소 소주의 VBN이높게 나타났으며(P<0.05) 처리구 모두 실험기간 동안 가식권 범위내에 있었다. 무기물 함량은 가약 흑염소소주가 순 흑염소 소주보다 높은 함량을 나타내었고, 한약재 증탕액에서 높은 함량을 나타내었던 무기물이 가약 흑염소 소주에서 높은 함량을 나타내었다. 특히 가약 흑염소 소주는 순 흑염소 소주도다 Ca, K, Mg가 2배이상 많은 함량을 나타내었다. 저장기간이 경과함에 따라 Ca, Fe는 감소하는 경향이었다. 순 흑염소 소주는 가약 흑염소소주보다 총 아미노산 함량이 높았으며, 가약 흑염소 소주는 glycine, glutamic acid, alanine, aspartic acid 순이었고, 순 흑염소 소주는 glycine, glutamic acid, alanine, aspartic acid, arginine 순이었으며, 한약재 증탕액은 gluta-mic acid와 aspartic acid 함량이 가장 높은 경향이었다.

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Studies on the Mucilage for the Manufacture of Korean Hand-Made Paper -Part II. Detection of Amino acids in the Mucilage of Ulmus coreana, Nakai Root- (한지(韓紙) 초조용(抄造用) 점액(粘液)에 관한 연구(硏究) -제2보(第二報). 느릅나무근(根) 점액(粘液)의 아미노산류(酸類)의 검색(檢索)-)

  • Sohn, Joo-Hwan;Im, Jai-Bin
    • Applied Biological Chemistry
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    • v.25 no.3
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    • pp.173-176
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    • 1982
  • The amino acids in the mucilage and its hydrolyzed products from Ulmus coreana, Nakai root were detected by thin layer chromatography. The mucilage contained 10 kinds of amino acids; alanine, aspartic acid, glutamine, glutamic acid, hydroxyproline, isoleucine, leucine, proline, phenylalanine and tyrosine. In the hydrolyzed mucilage 14 kinds of amino acids were detected; alanine, aspargine, arpartic acid, glutamine, glutamic acid, hydroxyproline, isoleucine, leucine, methionine, proline, phenylalanine, serine, tyrosine and valine.

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Content Analyses of Fiber, Protein and Amino Acids of Fully Ripe Fruits of Korea Native Squash, Cucurbita moschata Poir (한국재래종 호박 완숙과의 섬유질, 단백질 및 아미노산 함량 비교분석)

  • Youn, Sun-Joo;Jun, Ha-Joon;Kang, Sun-Chul
    • Applied Biological Chemistry
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    • v.47 no.4
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    • pp.403-408
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    • 2004
  • We studied active substances like crude cell wall components, crude protein, composing amino acids and free amino acids including orinithine cycle-related amino acids such as asparagine, ornithine and citrullin in fully ripe fruits of Korean native squash, Cucurbita moschata Poir. Crude protein content of 'Jeju 2' was the highest with $2,830\;{\mu}g/g$, while 'Sangju' was the lowest with $1,319\;{\mu}g/g$. Regarding the contents of crude cell wall components, fruit 'Kanghaw' was the highest with 2,961 mg% while 'Namhea' was the lowest with 1,582 mg%. Pectin contents of crude cell wall components were the highest in 'Kanghaw' (2,198 mg%) followed by 'Jeju 2' (2,178 mg%) and 'Jeju l' (1,461 mg%). The main contents of amino acids in squash were glutamic acid, aspartic acid, lysine, leucine and valine, which comprised to be more than 50% of total amino acid contents. Especially, in 'Jeju 2' aspartic acid and threonine were not detected. In fully ripe fruits, a total of 34 kinds of free amino acids were detected including 8 kinds of essential amino acids (histidine, isoleucine, leucine, lysine, phenylalanine, methionine, threonine and valine). More than 50% of the total free amino acids were aspartic acid and asparagine, and also all varieties were detected in ornithine, citrullin, and arginine, which are related to Ornithine cycle. There was a big difference in the contents of arginine in all varieties whereas the contents of ornithine and citrullin were very similar. 'Teaan' 29.34% was 7 times higher than 'Namhea' 4.30% in regards to arginine contents.

Studies on the Ecological Characteristics of Marine Bacteria Isolated from Deep Sea (심해 해양미생물의 분리 및 분리균의 생태적 특징에 관한 연구)

  • LEE Won-Jae;OHWADA Kouichi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.4
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    • pp.401-411
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    • 1995
  • Flavobacterium spp., Pseudomonas spp., and Vibrio spp. were isolated from samples(sediments) of Sagami Bay and Suruga Bay(in Japan) at 810-4,000m in depth. Among isolated strains, Vibvio sp.-86 and sp.-87 strains were identified as barophilic and psychrophilic ones. They grew in 400 atm and showed best growth at 100 atm. Marine bacteria grown at 400 atm were long rod shape and 30 to 50times longer than those grown at 1 atm. which were short rod shape and formed flocks (aggregates). Vibrio sp,-86 strain grew at $5-37^{\circ}C\;and\;0,5-9.0\%\;NaCl\;(3.0\%\;of\;optimum\;concentration),$ while Vibrio sp.-87 strain grew at $1-7\%\;NaCl\;(2,0\%\;of\;optimum\;concentration).$ The fatty acid compositions of Vibrio sp.-86 strain grown at 1 atm were $C_{20}-C_{22:0},\;C_{l6:1},\;and\;C_{16:0}$ in the order of their abundance and at 400 atm the order were $C_{18:1},\;C_{18:0},\;and\;C_{20}-C_{22}$, whereas those of Vibrio sp.-87 strain at 1 atm were $C_{6:1},\;C_{14:1},\;and\;C_{20}-C_{22}$ and at 400 atm the order were $C_{14:1},\;C_{12:0},\;and\;C_{16:1}$ The amino acids compositon of Vibrio sp.-86 strain grown at 1 atm were abundant in the order of aspartic acid, methionine, and glutamic acid and those at 400 atm were abundant in the order of methionine, glutamic acid, and aspartic acid. The amino acids composition of Vibrio sp.-87 strain grwon at 1 atm were abundant in the order of methionine, glutamic acid, and aspartic acid and those at 400 atm were abundant in the order of methionine, glutamic acid, and isoleucine.

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Changes of Ingredient in Traditional Andong Sikhe using Lactic Acid Bacteria and Yeast (젖산균 및 효모를 이용한 전통 안동식혜의 성분 변화)

  • Kim, Sung;Lee, Son-Ho;Choi, Hee-Jin;Jo, Guk-Young;Choi, Cheong
    • Korean Journal of Food Science and Technology
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    • v.30 no.6
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    • pp.1388-1393
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    • 1998
  • Traditional Andong sikhe was produced by fermenting L. bulgaricus LBS 47 and S. cerevisiae SCS 5. The changes of nitrogen compound and amino acid during fermentation and storage were investigated. Crude protein was increased until 4days, the main fermentation period. Amino form nitrogen increased up to 37.50 mg% at the 2nd day of fermentation and the product tasted best at this time. Water soluble and salt soluble protein decreased during fermentation. Proline and aspartic acid were the two major free amino acids. The free methionine increased while the free lysine decreased in the process of fermentation. The amino acids of water soluble protein and salt soluble protin were totally 17 kinds. The major amino acids of water soluble and salt soluble protein were glutamic acid and aspartic acid. The arginine content of salt soluble protein increased as the fermentation proceeded.

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Chemical Characteristics of Normal and Inferior Parts in Korean Red Ginseng (정상홍삼과 불량홍삼의 화학적 특성)

  • 김천석;이종원;도재호
    • Journal of Ginseng Research
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    • v.25 no.2
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    • pp.89-93
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    • 2001
  • This study was carried out to investigate a point of difference in chemical characteristics between normal and inferior Korean red ginseng (Naeback red ginseng=red ginseng with white part of clear boundary in phloem and/or xylem of ginseng body, saengnaeback red ginseng=red ginseng with white part of indistinct boundary). content of total sugar as chemical components of naeback and saengnaeback part from Korean red ginseng were less than that of normal part, and content of reducing sugar in normal and saengnaebakc part showed higher than that of naeback part. But differences in content of total phenolic compounds was not found. The content of crude saponin in normal part was highest, amounts of ginsneoside were about same. The content of constituent amino acid in normal part was about 2 times as compared with those in naeback and saengnaeback part in red ginseng. Among the various amino acids, the contents of arginine, glutamic acid, aspartic acid, leucine and alanine of normal and naeback part were higher than others, but in saengnaeback proline, glutamic acid, aspartic acid, threonin and glycine etc. were higher. In the total amount of free amino acids, those in normal, naeback and saengnaeback part were about the same one another, and arginine, glutamic acid and aspartic acid were major free amino acids.

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Comparative Study on Amino Acid Profiles of Wild and Cultured Carp, and Israeli Carp (천연 및 양식산 잉어와 이스라엘 잉어의 구성아미노산에 대한 비교연구)

  • CHOI Jin-Ho;RHIM Chae-Hwan;CHOI Yeung-Joon;PARK Kil-Dong;OH Sung-Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.6
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    • pp.545-549
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    • 1985
  • The muscles of wild and cultured carp (Cyprinus carpio), and Israeli carp (Cyprinus carpio nudus) were analyzed for the amino acid profiles, in vitro digestibility, predicted digestibility, C-PER and DC-PER. The distribution patterns of the total amino acid profiles in wild and cultured carp were found to be very similar, although there were relatively large differences in lysine, aspartic acid and glutamic acid contents which were higher in the cultured carp. Israeli carp was 2 times as much as wild carp in lysine. The quality of muscle proteins in wild and cultured carp, and Israeli carp was predicted by the in vitro method. The protein quality of Israeli carp was higher than that of wild and cultured carp, whereas there showed a similar tend to protein quality between wild and cultured carp. The contents of hitidine, tyrosine, aspartic acid, alanine and glutamic acid in diet showed stronger positive corelation with the levels of protein deposition than those of protein intake, and those amino acids would greatly influence on amino acid composition of muscle protein in fishes.

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