• 대한한방내과학회지
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• 제26권4호
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• pp.828-835
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• 2005

Objectives : Boo-ja and Cheon-o are not commonly prescribed, but are necessary for some clinical conditions, dispite the fact that some negative effects have been known to occur with these medicines. Hence, consequences for aspartate aminotransferase(AST), alanine aminotransferase(ALT) due to herbal medicines, including boo-ja and cheon-o are here reported. Methods : From January 2005 to July 2005, results were analyzed for 36 inpatients belonging to the sixth internal medicine department of Dongeui Oriental Medical Hospital who took herbal medicine, including boo-ja and cheon-o, over 20 days. The study is about the comparison and the investigation of LFT, and all results were taken upon their hospitalization and upon their departure. The standard of liver injury was used as a standard for their examinations. Results were as follows : No Change in normal limit : 28 of the 36 Increased AST. ALT from when they were hospitalized : 2 of 36 Normalized AST. ALT from when they were hospitalized with results higher than normal : 6 of 36 These results show no side-effects, therefore suggest that these herbal medicines, including boo-ja and cheon-o, do not cause adverse side-effects for patients of this kind if administered as was done in this study.

• Natural Product Sciences
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• 제22권4호
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• pp.231-237
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• 2016

Prolonged alcohol consumption causes alcoholic liver damage due to the generation of reactive oxygen species, the accumulation of fatty acids, and an increase in inflammatory cytokines in the liver. In this study, the protective effect of a fruit extract of Paeonia anomala (FEPA) against chronic alcohol-induced liver damage was evaluated in Sprague-Dawley rats fed an ethanol or a control Lieber-DeCarli diet for 5 weeks to induce alcoholic liver damage. FEPA (50, 25, and 10 mg/kg body weight/day) as well as the reference control silymarin (25 mg/kg body weight/day) were administered along with the ethanol diet. FEPA protected against increases in alanine aminotransferase and aspartate aminotransferase in serum and attenuated alcohol-induced increases in triglycerides, tumor necrosis factor alpha, thiobarbituric acid-reactive substances, and cytochrome P450 2E1 enzyme activity in the liver compared with the group treated with ethanol only. Anti-oxidative defenses such as the total glutathione level and glutathione peroxidase activity were increased by FEPA treatment. These results suggest that FEPA exerts protective effects against chronic alcohol-induced liver damage by attenuating hepatosteatosis and pro-inflammatory cytokine production and enhancing anti-oxidative defense mechanisms in the liver.

• Clinical and Experimental Pediatrics
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• 제54권7호
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• pp.292-297
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• 2011

Purpose: Our study aimed to evaluated sex differences in clinical features of obese high school students. Methods: One hundred three obese high school students (body mass index [BMI]${\geq}$85th percentile) and 51 control students (BMI<85th percentile) were enrolled in this study. Anthropometric measurements were performed. Fasting serum glucose, insulin, aspartate aminotransferase, alanine aminotransferase, total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and high-sensitive C-reactive protein were measured. Abdominal fat thickness, degree of fatty liver, and carotid intima-media thickness were measured by ultrasound. Results: In control and obese groups, waist circumference was significantly longer in boys but body fat mass was Significantly higher in girls. In the control group, total cholesterol and LDL-C were higher in girls. In the obese group, however, aspartate aminotransferase, alanine aminotransferase and triglyceride were higher and HDL-C was lower in boys. Preperitoneal fat thickness was significantly higher in obese girls. In obese group, the degree of fatty liver was significantly higher in boys. Carotid intima-media thickness was not significantly different between boys and girls. Conclusion: Obese adolescents had distinguishable sex differences in body measurements, metabolic abnormalities, abdominal fat thickness and fatty liver. We can infer that these characteristics may extend into adult obesity.

• 한국응용과학기술학회지
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• 제36권4호
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• pp.1420-1426
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• 2019

Dawley 계 숫컷 흰쥐(200~210g)를 정상군, CCl₄-대조군, CCl₄-실험군으로 나누어 실험군은 애엽 에탄올 추출물을 1,000mg/kg,b.w의 용량으로 1일1회7일간 경구 투여 후 사염화탄소를 0.6mg/kg,b.w의 용량으로 복강내 주사 후 다음날 개복하여 혈청내의 Alanine aminotransferase(ALT), Aspartate aminotransferase(AST),Alkaline phosphatase(ALP), Glutamyltranspeptidase(γ-GT), Lactate dehydrogenase(LDH)의 활성도와 중성지방, 콜레스테롤 함량을 측정한 결과 애엽 추출물 투여군에서 CCl₄-대조군과 비교하여 유의적인 감소를 나타내었으며, HDL-콜레스테롤은 유의적인 증가를 나타내었다. 간조직중의 지질과산화 함량은 감소를 glutathione함량은 유의적인 증가를 나타내었다 이 실험 결과 애엽 에탄올 추출물은 사염화탄소 투여에 의한 간 손상을 억제하는 보호물질을 함유하고 있음을 알 수 있었다.

• 한국식품위생안전성학회지
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• 제26권4호
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• pp.370-376
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• 2011

본 연구에서는 한방에서 약용으로 사용하고 있는 미나리과에 속하는 천궁을 에탄올 추출을 사용하여 고지방식 이에 의해 유발된 고지혈 및 비만 흰쥐에서의 항산화 활성과 간 보호 효과에 미치는 영향을 관찰하였다. 고지방 식이에 의해 유도된 고지혈 및 비만 흰쥐에 있어서의 간 손상에 대한 천궁 추출물의 보호효과를 연구한 결과 고지방 식이를 먹인 흰쥐의 혈청 aspartate aminotransferase (AST)와 alanine aminotransferase (ALT)는 증가 되었고, 간조직의 균질액에서 과산화지질의 최종 산물인 MDA도 높게 나타났다. 이와 반대로 천궁 추출물을 처치한 군에서는 간 기능 지표효소의 증가를 억제시켰다. 간조직의 항산화 효소인 SOD, catalase와 GPx의 활성은 고지방식이 의해 감소되었고, 천궁 추출물의 투여로 인해 효소 활성도 가증가됨을 볼 수 있었다. 본 연구를 통해 천연물을 이용한 기반연구로서 향후 천궁의 지질개선 효과와 항산화 효과에 대한 분자생물학적 조직병리학적 후속연구가 필요하다고 사료되며 이 연구의 결과를 바탕으로 천궁의 항산화 식품이나 지질개선 기능성 식품소재로의 활용이 기대되어진다.

• 대한본초학회지
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• 제21권3호
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• pp.47-54
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• 2006

Objectives : This study was performed to compare the preventive effect of water extracts from Injinho-tang and Haninjinho-tang on hyperlipidemia and liver damage induced by alcohol. Methods : Except for the normal group, we fed rat on 25% alcohol for 55 days. And each extract was administrated for the same period. We measured the serum component in rat's blood, body weight and weight of liver. Results : 1. At first, we observed effects of Injinho-tang and Haninjinho-tang on hyperlipidemia induced by alcohol. Injinho-tang group showed significant decrease of triglyceride, body weight in comparison with those of the control group. Haninjinho-tang group showed significant decrease of total cholesterol, triglyceride in comparison with those of the control group. I 2. At second, we observed effects of Injinho-tang and Haninjinho-tang on liver damage induced by alcohol. lnjinho-tang group showed significant decrease of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALP (alkaline phosphatase), liver weight in comparison with those of the control group. Haninjinho-tang group showed significant decrease of AST (aspartate aminotransferase), ALT (alanine aminotransferase) in comparison with those of the control group. Conclusion : Reviewing these experimental results, it appears that water extracts from Injinho-tang and Haninjinho-tang have pharmaceutical preventive efficacy on hyperlipidemia and liver damage induced by alcohol. Therefore further additional study should be conducted to elucidate in depth the pharmaceutical efficacy of these.

• 생물정신의학
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• 제5권2호
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• pp.235-242
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• 1998

Objectives : Alcohol-induced oxidative stress has been known to injure various tissues or organs. This stress is related with free radicals which are produced as the result of long-term alcohol consumption. Malonyldialdehyde(MDA) is produced by the interaction of free radicals and cell membrane lipids, and indicates the degree of lipid peroxidation indirectly. The purpose of this study was to investigate the relationship between red blood cell(RBC) membrane lipid peroxidation by free radicals, and associated hepatic injuries and hematologic changes. Methods : Thirty-three subjects diagnosed as alcohol dependence according to DSM-IV diagnostic criteria were evaluated within 72 hours after discontinuing alcohol drinking. Clinical characteristics were evaluated by CAGE questionnaire and Korean Michigan Alcoholism Screening Test(MAST). RBC membrane MDA level was measured as the marker of RBC membrane lipid peroxidation. Aspartate aminotransferase(AST), alanine aminotransferase(ALT) and gamma-glutamyltransferase(GGT) were used as the biochemical markers of liver damage due to alcohol ingestion. The alcohol-induced hematologic change was assessed by mean corpuscular volume(MCV). Results : The results were as follows. Clinical characteristics were not different between two groups having normal and abnormal levels of AST, ALT, GGT or MCV. The levels of MDA were not correlated with the clinical characteristics and serum levels of AST, ALT and GGT. However, there was a significant correlation between the levels of MDA and the value of MCV(p=0.017). Conclusions : These findings suggest that oxidative stress in alcohol dependence may not be reflected in liver enzyme markers such as AST, ALT and GGT, but may be reflected in MCV.

• 한국식품위생안전성학회지
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• 제15권1호
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• pp.51-54
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• 2000

1. E.coli에서 별도의 expression vector를 사용하지 않고도 Bacillus 균주 유래의 유용효소(D-Amino acid aminotransferase;D-AAT, Aspartate aminotransferase;AspAT, Alanine dehydrogenase;AlaDH 등)의 유전자의 5'-up stream부위의 발현 기구를 검토한 결과 각 유용 효소의 유전자들의 5'-upstream부위에 존재하는 프로모터들이 연속적으로 존재한다는 사실이 유추되었고, E.coli의 SD sequence와 매우 상동성이 높은 서열 또한 존재함을 확인하였다. 2. 유용 효소 유전자의 번역과 관련된 5'-upstream 부위의 분석을 통하여 유용 효소의 유전자들은 E.coli의 ribosomal RNA와 매우 안정한 SD pairing을 형성(D-AAT의 경우: -13.0kcal/mol, AspAT의 경우; -9.5kcal/mol, AlaDH의 경우 -15.8kca1/mol)할 수 있음을 확인하여, 이러한 높은 자유에너지 변화는 E.coli내에서 유용 효소의 번역에 기여함을 예상할 수 있다.

• Journal of Acupuncture Research
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• 제18권6호
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• pp.188-205
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• 2001

Objective : The purpose of this studies was to determine the effect of Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. on changes of trabecular area and physiological metabolites in the ovariectomized osteoporotic Rats. Methods : In order to induce estrogen deficient osteoporosis, ovariectomy was done on rats. Then the Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. were orally administerd: Such indexes were measured as the changes of body weight, bone mineral density, trabecular area in tibia, and levels of osteocalcin, bone alkaline phosphatase, calcium, phosphorus, cholesterol, triglyceride, aspartate aminotransferase and alanine aminotransferase in serum. Results : 1. The change of bone mineral density in Bogol-Tang group and Rhynchosia volubilis lour. group. was significantly increased compare to control group. 2. The change of trabecular area % in epiphysis of tibia in Carthami semen group was significantly increased compare to control group. 3. The change of trabecular area % in diaphysis of tibia in Bogol-Tang group was significantly increased compare to control group. 4. The change of serum osteocalcin in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 6. The change of1 serum calcium in Rhynchosia volubilis lour. group was significantly decreased compare to control group. 7. The change of phosphorus in Carthami semen group and Rhynchosia volubilis Lour. group was significantly decreased compare to control group. 8. The change of serum cholesterol and triglyceride of experimental groups were decreased in comparison with control group. 9. The change of serum AST(Aspartate aminotransferase : GOT) in Rhynchosia volubilis Lour. group was decreased compare to control group. 10. The change of serum ALT(Alanine aminotransferase : GPT) in Bogol-Tang group was decreased compare to control group.

• 미생물학회지
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• 제45권4호
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• pp.416-418
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• 2009

번데기동충하초의 간독성 회복 효과를 HaM/ICR 계열의 흰쥐를 이용하여 검정하였다. 벤조피렌 복강주사시, 혈중의 aspartate aminotransferase (AST) 및 alanine aminotransferase (ALT)의 효소활성과 간조직의 lipid peroxide (LPO) 농도는 대조군과 비교시 급격히 증가하여 간독성을 유발하였다. 그러나, 벤조피렌 대조군과 비교시, 번데기 동충하초 배양액의 경구 투여시 AST와 ALT의 효소활성 및 LPO 농도는 감소하였으며, 벤조피렌에 유도된 간 조직의 glutathione 농도 및 glutathione S-transferase 효소활성은 증가하여 간독성을 회복하였다. 실험결과는 번데기 동충하초 균사체 배양액이 벤조피렌 유발 간독성에 대해 회복 효과가 있음을 보여준다.