한국응용과학기술학회지 (Journal of the Korean Applied Science and Technology)

  • 제36권4호
  • /
  • Pages.1420-1426
  • /
  • 2019
  • /
  • 1225-9098(pISSN)
  • /
  • 2288-1069(eISSN)
한국응용과학기술학회 (The Korean Society of Applied Science and Technology)
권호 표지
DOI QR코드

DOI QR Code

애엽 에탄올 추출물이 사염화탄소로 유발된 흰쥐의 간 손상 보호효과

Protective Effect of Ethanol Extract of Artemisiae vulgaris L. on hepatic injury Induced by Carbon tetrachloride In Rat.

  • 김옥경 (대진대학교 과학기술대학 식품영양학과)
  • Kim, Ok-Kyung (Department of Food Science and Nutrition, Dae Jin University,)
  • 투고 : 2019.11.25
  • 심사 : 2019.12.29
  • 발행 : 2019.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Dawley 계 숫컷 흰쥐(200~210g)를 정상군, CCl4-대조군, CCl4-실험군으로 나누어 실험군은 애엽 에탄올 추출물을 1,000mg/kg,b.w의 용량으로 1일1회7일간 경구 투여 후 사염화탄소를 0.6mg/kg,b.w의 용량으로 복강내 주사 후 다음날 개복하여 혈청내의 Alanine aminotransferase(ALT), Aspartate aminotransferase(AST),Alkaline phosphatase(ALP), Glutamyltranspeptidase(γ-GT), Lactate dehydrogenase(LDH)의 활성도와 중성지방, 콜레스테롤 함량을 측정한 결과 애엽 추출물 투여군에서 CCl4-대조군과 비교하여 유의적인 감소를 나타내었으며, HDL-콜레스테롤은 유의적인 증가를 나타내었다. 간조직중의 지질과산화 함량은 감소를 glutathione함량은 유의적인 증가를 나타내었다 이 실험 결과 애엽 에탄올 추출물은 사염화탄소 투여에 의한 간 손상을 억제하는 보호물질을 함유하고 있음을 알 수 있었다.

This study was done to investigate the protective effects of ethanol extract Artemisiae vulgaris L(Av) on carbon tetrachloride(CCl4)intoxicated rats. Male sprague Dawley rats(200~210g)was used. experimental groups were divided into normal group, CCl4-control group, and ethanol extract CCl4-treated group. CCl4-treated groups were injected with CCl4 0.6mg/kg.b.w(i.p). The activities of Alanine aminotransferase(ALT), Aspartate aminotransferase(AST), Alkaline phosphatase(ALP), Glutamyl transpeptidase(γ-GT), Lactate dehydrogenase(LDH) in extract pretrated group was significantly decreased(p<0.05) compared to the CCl4-control group. The contents of triglyceride, cholesterol and lipid peroxide were significantly decreased(p<0.05). whereas the contents of HDL-cholesterol and glutathione(GSH) were significantly increased(p<0.05). These results suggest that extract of Artemisiae vulgaris L(Av) has hepatoprotective effect in the CCl4-intoxicated rats.

키워드

참고문헌

  1. J. Neuzil, J. Gebick, and R. Stocker, "Radical induced chain oxidation of proteins and its inhibition by chain breaking antioxidants". Journal of Biochemical, Vol.293, No.1, pp.601-606 (1993). https://doi.org/10.1042/bj2930601
  2. D. Steinberg, S. Pathasarathly, T. E. Carew, J. C. Khoo, and J. L Witztum "Beyond cholesterol. Modifications of low density lipoproteins that increase its atherogenicity" England. Journal of Meicine. Vol.320, No.3, pp.915-923 (1989). https://doi.org/10.1056/NEJM198904063201407
  3. W. Gary, and D. Cynthia "Leaf The role of oxidative stress in HIV disease. free radical". Journal of Biologycal and Medicine. Vol.19, No.2, pp.523-528 (1995).
  4. N. Manabe, M. Sugimoto, Y. Azuma, N. Taketome, A. Yamashita, H. Tsuboi, A. Tsunoo, N. Kinjo. "Effects of the nycelial extract of cultured cordces sinensis on in vivo hepatic energy metabolism in the mouse". Journal of Japaness. Pharmacology. Vol.70, No.7, pp.85-88 (1996). https://doi.org/10.1254/jjp.70.85
  5. S. N. Ryu, S. S. Han, J. J. Yang, H. G. Jeong, S. S. Kang. "Variation of eupatilin and jaceoside content of mugwert". Journal of Crop Science, Vol.50, No.10, pp.204-207 (2005).
  6. S. J. Lee, H. Y. Chang, I. K. Lee, I. D. Yoo. "lsolation and ldentification of Flavonoids from Ethanol Extracts of Artemisia vulgaris and Their Antioxidant Activity". Korean Journal Food. Science Technology. Vol.31, No.3, pp.815-822 (1999).
  7. Y. Koshihara, T. Neichi, S. Murota, A. Lao, Y. Fujimoto, T. Tatsuno. "Selective inhibition of 5-lipoxygenase by natural compounds isolated from chinese plants, Artemisia rurips Naki" FEBS Letters, Vol.158, No. 10, pp.41-44 (1983). https://doi.org/10.1016/0014-5793(83)80672-3
  8. C. K. Lee, J. J. Seo. "Antimicrobial Activity of the Aerial part of Artemisia capillaris Extracts on the Food-Borne Pathogens". Journal Korean Society Food Science and Nutrition, Vol.32. No.8, pp.1227-1232 (2003). https://doi.org/10.3746/jkfn.2003.32.8.1227
  9. S. S. Yun. "Effect of aqua-acupuncture of Artemisia capillaris herba extract on the rat hepatocyte damaged by carbon tetrachloride". MS Thesis. Daejeon University, pp.21-40 (1991).
  10. Y. X. Jin, Y. S. Yoo, E. K. Han, I. J. Kang, C. K Chung. "Artemisia capillaris and paecilomyces Japonica stimulate Lipid Metabolism and Reduced Hepatotoxicity Induced by Carbon Tetrachloride in Rats". Journal Korean Society Food Science and Nutrition Vol.37, No.5, pp. 548-554 (2008). https://doi.org/10.3746/jkfn.2008.37.5.548
  11. K. N. Kim, J. A. Lee, W. J Yoon, J. Y. Kim, G. P Song, S. Y. Park. "The Cytotoxicity of Artemicia fukudo Extracts Against HL-6-Cells". Journal Korean Society Food Science and Nutrition, Vol.36, No.7, pp.819-824 (2007). https://doi.org/10.3746/jkfn.2007.36.7.819
  12. V. C. Rao and H. M. Nehendale, "Colchicine Antimiosis Abolishes $CCl_4$ Autoprotection", Toxicology and Pathology, Vol.19, No.8, pp.597-599 (1991). https://doi.org/10.1177/019262339101900405
  13. O. K.Kim : "The Effects of Sedum sarmentosum Bunge Extract using Super Critical Carbon Dioxide on Lipid Metabolism, Lipid Peroxidation and Antioxidation in Carbon Tetrachloride Induced Hepatotoxicity in Rats". Journal of Korean Oil Chemist's Society, Vol.21, No.1, pp.204-213(2004).
  14. A. W. Hayes, "Principles and Methods of Toxicology", pp.407-410, Raben Press, New York (1982).
  15. S. B, Lee, C. Jeong, S. H Jeong, S. M. Lee, S. B. Shim, T. S. Cho. "hepatoprotective effects of extracts from artemisia iwayomogi". Journal of Applied. Pharmacology. Vol.20, No.5, pp.194-201 (1997).
  16. T. Y. Chen, C. F. Lu, Q. Y. Hu, S. X. Wang H. Tan. "protective effect of Yinchenhae Tang on hepatocytes of rats with experimental hepatic fibrosis and ultrastructural findings" Heilongjiang Medicine Pharmacology, Vol.27, No.9, pp.11-12(2004).
  17. J. B. Whitfield, R. E. Pounder, G. Neale, and D. W. Moss, "Serum ${\gamma}$ -Glutamyl Transpeptidase Activity in Liver Disease" Gut, Vol.13, No.2, pp.702-705 (1972). https://doi.org/10.1136/gut.13.9.702
  18. E. G. Han and S. Y. Cho, "Effect of Codonopsis lanceolata Water Extract on the Activities of Antioxidative Enzymes in Carbon Tetrachloride Treated Rats" Journal. Korean society Food Science and Nutrition Vol.26, No.5, pp.1181-1185 (1997).
  19. A. W. Hayes, "Principles and Methods of Toxicology" pp.407-409, Raben Press, New York (1982).
  20. S. M. Nam, S. S. ham, D. H. OH, I. J. kang, S. Y. Lee, C. K. Chung. "effects of Artemisia iwayomogi Kitamura ethanol extract on lowering serum and liver lipids in rats" Journal. Korean society Food Science and. Nutrition, Vol.27, Vol.4, pp.338-343(1998).
  21. R. O. Recknagel, E. A. Glende, and A. M., Hruszkewyez, "Chemical Mechanism in Carbon Tetrachloride Toxicity. In Free Radicals in Biology", W. A. Prayor (ed), pp.97-99, Academic Press, New York (1997).
  22. M. J. Burkitt and J. Duncan, "Effects of Trans Resveratrol on Copper Dependent Hydroxyl Radical Formation and DNA Damage : Evidence for Hydroxyl Radical Scavening and a Novel, Glutathione Sparing Mechanism of Action", Archives of Biochemistry and Biophysics, Vol.381, No.8, pp.253-260 (2000). https://doi.org/10.1006/abbi.2000.1973