• 미생물학회지
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• 제32권4호
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• pp.285-290
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• 1994

자연계에서 새로운 제한효소 생산균을 검색하여 한 균주를 선발하고, 형태학적, 생리학적, 생화학적 특성들을 조사하여 Alcaligenes sp.로 동정하고 제한효소의 특성을 조사하였다. Alcaligenes sp. J-482가 생산하는 제한효소를 AspJI으로 명명하였다. AspJI은 pBR322, Adenovirus 2-DNA, ${lambda}$ DNA 등에 대한 절단양식이 AatII와 같아 AatII의 isoschizomer로 추정 되었으며, 효소활성에 12.5mM 이상의 $MgCl_2$를 필요로 하였으며, NaCl에 의하여 저해되었다. AspJI의 반응 최적 온도는 $37^{circ}C$, 최적 pH는 7.5로 확인 되었으며, 내열성을 조사한 결과 $85^{circ}C$이상에서 15분처리 할 때 안전히 실활되는 것으로 관찰되었다.

• 대한핵의학회:학술대회논문집
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• 대한핵의학회 2003년도 추계학술대회 및 총회
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• pp.15.2-15.2
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• 2003

• 약학회지
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• 제36권5호
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• pp.469-473
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• 1992

Yeast alcohol dehydrogenase (YADH) has an acidic residue that interacts with the 2'- and 3'-hydroxyl groups of the adenosine ribose of the $NAD^+$ coenzyme. The acidic residue of Asp-223 (according to horse liver alcohol dehydrogenase amino acid sequence) is supposed to determine the coenzyme specificity for $NAD^+$ rather than $NADP^+$. We mutated Asp-223 to leucine and the mutant YADH was expressed in yeast and characterized for the coenzyme specificity. The turnover numbers of mutant enzyme for $NAD^+$ and ethanol were decreased 3.5- and 4.8-fold compared to wild-type enzyme, respectively. Contrastively, catalytic specificity for $NADP^+$ was increased 13-fold. As a result, the mutant YADH also employed $NADP^+$ as a coenzyme.

• 동아시아식생활학회지
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• 제14권4호
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• pp.363-369
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• 2004

Three different doengjang(fermented soybean paste) were prepared by using B. subtilis SS103 with high angiotensin converting enzyme(ACE) inhibitory activity, Asp. oryzae and mixed culture of two organisms, respectively. Quality characteristics and the inhibitory activity were compared. $\beta$-Amylase activity rapidly increased during 30 days of fermentation, but lower activity was found in doenjang prepared with B. subtilis. However, $\alpha$-Galactosidase activity decreased for 15 days of fermentation and little activity was observed after that fermentation period. Protease production tended to increase during the fermentation. High protease activity was found only in doengjang prepared with Asp. oryzae, but the doengjang prepared with B. subtilis SS103 showed very low activity. Total isoflavone content at 90 day fermentation was the highest in doengjang prepared with mixture of Asp. oryzae and B. subtilis. ACE inhibitory activity increased during the fermentation period. The highest activity was found in doengjang made with mixture of Asp. oryzae and B. subtilis. Sensory evaluation on doengjang itself and its soup indicated that doengjang made with mixture of Asp. oryzae and B. subtilis showed higher acceptability on taste, flavor and color than those prepared with only Asp. oryzae or B. subtilis, respectively.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.646-647
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• 2001

The enzymatic synthesis of conjugates of lysine and aspartic acid with D-glucose was studied. Optimase M-440 showed the very poor regioselectivity in the transesterification of $N{\alpha}$,$N'{\varepsilon}-di-t-Boc-L-Lys-OTFE$ and N-t-Boc-L-Asp-diOTFE with D-glucose. The acylation of glycosidic -OH and primary -OH of D-glucose occurred. However, Optimase M-440 catalyzed only the acylation of primary -OH group in the transesterification of $N{\alpha}$,$N'{\varepsilon}-di-t-Boc-L-Lys-OTFE$ and N-t-Boc-L-Asp-diOTFE with ${\alpha}-$ and ${\beta}-methylglucopyranoside$ in high yields without any other transesterification. Optimase M-440 also discriminated carboxyl groups of N-t-Boc-L-Asp-diOTFE.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.449-452
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• 2013

Polymorphisms in DNA repair genes have been shown to influence DNA repair processes and to modify cancer susceptibility. Here we conducted a case-control study to assess the role of potential SNPs of DNA repair genes on the risk of glioma and meningioma. We included 297 cases and 458 cancer-free controls. Genotyping of XRCC1 Gln399Arg, XRCC1 Arg194Trp, XRCC2 Arg188His, XRCC3 Thr241Met, XRCC4 Ala247Ser, ERCC1 Asn118Asp, ERCC2 Lys751Gln and ERCC5 Asp1558His were performed in a 384-well plate format on the Sequenom MassARRAY platform. XRCC1 Arg194Trp (rs1799782) and ERCC2 Asp312Asn rs1799793 did not follow the HWE in control group, and genotype distributions of XRCC1 Gln399Arg rs25487, XRCC2 Arg188His rs3218536 and ERCC2 Asp312Asn rs1799793 were significantly different between cases and controls (P<0.05). We found XRCC1 399G/G, XRCC1 194 T/T and XRCC3 241T/T were associated with a higher risk when compared with the wild-type genotype. For ERCC5 Asp1558His, we found G/G genotype was associated with elevated susceptibility. In conclusion, our study has shown that XRCC1 Gln399Arg, XRCC1 Arg194Trp, XRCC3 Thr241Met and ERCC5 Asp1558His are associated with risk of gliomas and meningiomas. This finding could be useful in identifying the susceptibility genes for these cancers.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.145-148
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• 2013

Aim: Individual differences in chemosensitivity and clinical outcome of non-small-cell lung cancer (NSCLC) patients may be induced by host inherited factors. We investigated the impact of XPD Arg156Arg, XPD Asp312Asn, XPD Asp711Asp and XPD Lys751Gln gene polymorphisms on the efficacy of platinum-based chemotherapy in NSCLC patients. Methods: A total of 496 were consecutively selected from the Affiliated Hospital of Nantong University between Jan. 2003 and Nov. 2006, and all patients were followed-up until Nov. 2011. The genotyping of XPD Arg156Arg, XPD Asp312Asn, XPD Asp711Asp and XPD Lys751Gln was conducted by duplex polymerase-chain-reaction with the confronting-two-pair primer methods. Results: Individuals with XPD 312 C/T+T/T and XPD 711 C/T+T/T exhibited poor responses to chemotherapy when compared with the wild-type genotype, with adjusted ORs(95% CI) of 0.67(0.38-0.97) and 0.54(0.35-0.96), respectively. Cox regression showed the median PFS and OS of patients of XPD 312 C/T+T/T genotype and XPD 711 C/T+T/T genotype to be significantly lower than those with wild-type homozygous genotype. Conclusion: We found polymorphisms in XPD to be associated with response to platinum-based chemotherapy in NSCLC, and our findings provide information for therapeutic decisions for individualized therapy.

• 대한물리치료과학회지
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• 제17권1_2호
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• pp.11-21
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• 2010

Background: This study was undertaken to evaluate how gender differences affect the sensory processing by the Adolescent/Adult Sensory Profile(ASP) which evaluates the behavioral responses of adults to everyday sensory experiences. Methods: The study period lasted one month which was June 2009, and the study subjects were 325(three hundred twenty five) people who were healthy and reside in Chungcheong province including Daejeon. Detailed information about the study was explained to the study group and the ASP questionnaires were drawn up by them personally. Results: On average, the study group scores similarly in low registration, sensation seeking, sensory sensitivity, and sensation avoiding. According to the sensory processing abilities by gender, the group obtained scores indicating performance similar to most people in low registration, sensation seeking, and sensory sensitivity, except for sensation avoiding. Furthermore, in the taste/smell processing of "I only eat familiar foods", sensation avoiding score was higher in females than males, and in the activity level of "I find time to get away from my busy life and spend time by myself", males' sensation avoiding scored higher than females. Conclusion: In the study of differences of sensory processing abilities of adults by gender using the ASP, gender differences were revealed in the category of sensation avoiding.

• 한국식품위생안전성학회지
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• 제15권2호
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• pp.122-127
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• 2000

아플라톡신 생성균의 생육을 억제할 수 있는 미생물을 분리동정하고 진공동결 건조한 대사산물로부터 길항물질을 분리하였다. 대두로부터 아플라톡신 생육을 저해하는 길항균을 분리하였으며, 그 균은 Bacillus subtilis로 동정되었다. Bacillus subtilis는 아플라톡신 생성균에 대한 길항물질을 생산하였으며 MeOH추출, 실리카겔 흡착 크로마토그래피법, Sephadex LH-20 크로마토그래피법으로 길항물질을 분리하였다. 아플라톡신 생성균인 Asp. flavus와 Asp. parasiticus의 생육은 길항미생물인 Bacillus subtilis가 생산하는 길항물질에 의해 강하게 저해되었으며 이는 아플라톡신 오염방지를 위하여 효과적인 생물학적 방법일 것이라 기대된다.

• 한국ITS학회:학술대회논문집
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• 한국ITS학회 2004년도 제3회 정기총회 및 추계학술대회
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• pp.365-369
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• 2004