• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.101.2-102
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• 2001

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.160.2-160.2
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.161.1-161.1
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• 2000

• Archives of Pharmacal Research
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• 제28권4호
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• pp.502-508
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• 2005

Titrated extract of Centella asiatica (TECA) contains three principal ingredients, asiaticoside (AS), asiatic acid (AA), and madecassic acid (MA). These components are known to be clinically effective on systemic scleroderma, abnormal scar formation, and keloids. However, one problem associated with administration of TECA is its low solubility in aqueous as well as oil medium. In this study, various nonionic surfactants and bile salts as anionic surfactant were tested and screened for solubilizing TECA with a view to developing topical hydrogel type of ointment which is stable physicochemically, and has better pharmacological effects. When TECA was incorporated into various nonionic surfactant systems, labrasol had the most potent capacity for solubilizing TECA. In cases of bile salt systems, Na-deoxycholate (Na-DOC) had foremost solubilizing capacity, even more than labrasol. In differential scanning calorimetric study, the peaks of AA, MA, AS and Na-DOC disappeared at the coprecipitate of $1\%$ TECA and $1\%$ Na-DOC, suggesting the optimum condition of Na-DOC for solubilizing TECA. When the physicochemical stability of hydrogel containing this mixture was assessed, it was stable at room temperature for at least one month. Pharmacologically it significantly decreased the size of wound area at the $9^{th}$ day when applied to the wound area of rat dorsal skin. Taken together, solubility of TECA was dramatically improved by using non ionic and anionic surfactant systems, and Na-DOC was found to be the most effective solubilizer of TECA in formulating a TECA-containing hydrogel typed ointment. Moreover this gel was considered to be applicable to clinical use for wound healing effect.

• 대한화장품학회지
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• 제45권4호
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• pp.341-351
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• 2019

본 연구에서는 참꽃나무 잎 추출물의 항산화 및 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. 참꽃나무 잎 에탄올 추출물 및 용매 분획물의 DPPH, ABTS⁺ 라디칼 소거 활성을 측정한 결과 추출물 및 ethyl acetate (EtOAc), n-butanol (BuOH) 분획물의 우수한 라디칼 소거 활성을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험 결과, 추출물 및 n-hexane (Hex), EtOAc 분획물에서 항균 활성이 나타났다. EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그래피를 수행하여 5개의 화합물을 분리하였다; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4), isoquercetin (5). 분리된 화합물 1-5는 모두 참꽃나무에서 처음으로 분리되었다. 분리된 화합물에 대한 항산화 활성 측정 결과 화합물 4, 5의 라디칼 소거 활성이 우수하였으며, 화합물 3은 항균 효과가 있음을 확인하였다. 또한 UPLC 분석을 통해 참꽃나무 잎에서 분리된 astragalin의 함량을 측정한 결과 추출물에서 8.1 mg/g, EtOAc 분획물에서 34.8 mg/g이 함유되어 있는 것으로 확인되었다. 이상의 연구 결과를 바탕으로 참꽃나무 잎 추출물을 이용한 항산화 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다.

• Archives of Pharmacal Research
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• 제28권10호
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• pp.1135-1141
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• 2005

Twelve compounds were isolated from the MeOH extract of the leaf and stem of the Korean endemic plant Weigela subsessilis L. H. Bailey. Their chemical structures were elucidated on the basis of physicochemical and spectroscopic data and by comparison with those of published literatures. These compounds were identified as three sterols, ${\beta}$-sitosterol acetate (2), ${\beta}$-sitosterol (3), daucosterol (11), eight triterpenoids, squalene (1), ursolic acid (4), ilekudinol A (5), corosolic acid (6), ilekudinol B (7), esculentic acid (8), pomolic acid (9), asiatic acid (10), and one iridoid glycoside, alboside I (12). This is the first report pertaining to the isolation of these compounds from Weigela subsessilis L. H. Bailey. In addition, three compounds 7, 9, and 12 were found to display a strong inhibitory effect on the production of IL-8 in the HT29 cells stimulated by TNF-${\alpha}$.

• Natural Product Sciences
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• 제24권3호
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• pp.213-218
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• 2018

Chemical investigation of the plant Tristemma hirtum P. Beauv (Melastomataceae) resulted to the isolation of a new flavonol glycoside named quercetin-7-O-${\alpha}$-D-arabinofuranoside (1), together with nine known compounds including 3'-hexadecanoyl-2'-(9aZ)-tetradecanoyl-glycerol 1'-O-[${\beta}$-D-galactopyranosyl-(1'' ${\rightarrow}$ 6'')-${\alpha}$-D-galactopyranoside] (2), arjunolic acid (3), ${\beta}$-sitosterol-3-O-${\beta}$-D-glucopyranoside (4), terminolic acid (5), quercetin (6), asiatic acid (7), maslinic acid (8), $1{\beta}$-O-galloylpedunculagin (9) and 6-hydroxyapigenin 7-O-${\beta}$-D-glucopyranoside (10) from the methanol extract using normal and reversed phase column chromatography. The structures of these compounds were determined by comprehensive interpretation of their spectral data mainly including 1D- 2D-NMR ($^1H-^1H$ COSY, HSQC, and HMBC) spectroscopic and ESI-TOF-MS mass spectrometric analysis.

• 대한화장품학회지
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• 제46권3호
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• pp.207-218
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• 2020

본 연구에서는 등수국 잎 추출물의 항염 및 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. RAW 264.7 세포를 이용한 항염 활성 실험 결과, n-hexane (Hex) 및 ethyl acetate (EtOAc) 분획물이 세포 독성 없이 nitric oxide (NO)의 생성 및 iNOS 단백질의 발현을 농도의존적으로 억제시키는 것을 확인하였다. 추가적인 항염 기전 연구 결과, n-Hex 및 EtOAc 분획물이 전염증성 cytokine (TNF-α, IL-1β, IL-6)의 생성을 억제하는 것을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험 결과, 추출물 및 n-Hex, EtOAc, n-butanol (BuOH) 분획물에서 항균 활성이 나타났다. n-Hex 및 EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그래피를 수행하여 4 개의 화합물을 분리하였다; phytol (1), corosolic acid (2), asiatic acid (3) 및 1-O-p-coumaroyl-β-D-glucopyranoside (4). 이들 화합물은 모두 등수국에서 처음으로 분리된 물질이다. 또한 HPLC 분석을 통해 등수국 잎 추출물에서 분리된 화합물의 함량을 측정한 결과 phytol (1) 이 27.8 mg/g 함유되어 있는 것으로 확인되었다. 이상의 연구 결과를 바탕으로 등수국 잎 추출물을 이용한 항염 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.171-171
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• 2003

Asiaticoside, a biologically active triterpenoid present in Centella asicatica, has been known to exert a variety of biological effect such as wound-healing, hepatoprotective, anti-inflammatory. In this study, we observed the effect of asiatic acid asiaticoside and its 16 derivatives on the cytotoxicity and the content of hydroxyproline in rat hepatic stellate cell line, HSC-T6 cell as a preliminary study for screening the anti-hepatofibrotic effect.(omitted)

• Natural Product Sciences
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• 제22권1호
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• pp.20-24
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• 2016

Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an $IC_{50}$ value of $64.6{\mu}g/mL$. In addition, the PRE inhibited tyrosinase enzyme activity with an $IC_{50}$ value of $104.8{\mu}g/mL$. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at $4^{\circ}C$. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.