Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Anti-oxidative and Anti-bacterial Constituents from the Extracts of Rhododendron weyrichii Leaves

참꽃나무 잎 추출물 유래 항산화 및 항균 활성 성분

  • Kim, Jung Eun (Department of Chemistry and Cosmetics, Jeju National University) ;
  • Jo, Seong Mi (Department of Chemistry and Cosmetics, Jeju National University) ;
  • Lee, Nam Ho (Department of Chemistry and Cosmetics, Jeju National University)
  • 김정은 (제주대학교 화학.코스메틱스학과) ;
  • 조성미 (제주대학교 화학.코스메틱스학과) ;
  • 이남호 (제주대학교 화학.코스메틱스학과)
  • Received : 2019.10.16
  • Accepted : 2019.12.02
  • Published : 2019.12.30
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Abstract

In this study, we investigated anti-oxidative and anti-bacterial constituents from Rhododendron weyrichii leaves. DPPH and ABTS+ radical scavenging activities were screened for the ethanol extract and solvent fractions. Potent scavenging activities were appeared from the extract, ethyl acetate (EtOAc) and n-butanol (BuOH) fractions. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, extract, n-hexane (Hex) and EtOAc fractions showed strong activities. To isolate the active constituents, the EtOAc fraction was further purified to afford five phytochemicals; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4) and isoquercetin (5). All of the compounds 1-5 were isolated for the first time from this plant. Among the isolates, the compound 4 and 5 showed strong DPPH and ABTS+ radical scavenging activities. Also, compound 3 exhibited the most potent anti-bacterial activity. In addition, the content of astragalin isolated from this plant was determined by UPLC and the quantity was about 8.1 mg/g for the 70% ethanol extract and 34.8 mg/g for the EtOAc fraction. Based on these results, it is concluded that R. weyrichii extract could be potentially applicable as anti-oxidative and anti-bacterial ingredients in cosmetic formulations.

본 연구에서는 참꽃나무 잎 추출물의 항산화 및 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. 참꽃나무 잎 에탄올 추출물 및 용매 분획물의 DPPH, ABTS+ 라디칼 소거 활성을 측정한 결과 추출물 및 ethyl acetate (EtOAc), n-butanol (BuOH) 분획물의 우수한 라디칼 소거 활성을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험 결과, 추출물 및 n-hexane (Hex), EtOAc 분획물에서 항균 활성이 나타났다. EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그래피를 수행하여 5개의 화합물을 분리하였다; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4), isoquercetin (5). 분리된 화합물 1-5는 모두 참꽃나무에서 처음으로 분리되었다. 분리된 화합물에 대한 항산화 활성 측정 결과 화합물 4, 5의 라디칼 소거 활성이 우수하였으며, 화합물 3은 항균 효과가 있음을 확인하였다. 또한 UPLC 분석을 통해 참꽃나무 잎에서 분리된 astragalin의 함량을 측정한 결과 추출물에서 8.1 mg/g, EtOAc 분획물에서 34.8 mg/g이 함유되어 있는 것으로 확인되었다. 이상의 연구 결과를 바탕으로 참꽃나무 잎 추출물을 이용한 항산화 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다.

Keywords

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