• 한국어병학회지
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• 제26권1호
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• pp.19-30
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• 2013

Megalocytivirus는 우리나라를 포함한 아시아 각국의 양식현장에서 고위험성 병원체이다. 그럼에도 불구하고 어류의 면역체계와 megalocytivirus의 상호관계에 관한 연구는 아직 부족한 실정이다. 다양한 연구에서 cyclooxygenase isoform중 COX-1 유전자는 constitutive 하게 발현되며, COX-2 enzyme은 면역반응에 중요한 역할을 한다고 알려져 있다. 또한 COX-2 유전자는 LPS (lipopolysaccharide) 또는 병원체의 감염과 같은 염증반응 시 그 발현이 증가한다고 알려져있다. 본 연구는 다른 어종의 COX-2 유전자를 바탕으로 제작된 degenerated primer와 5'- 그리고 3'-end RACE-PCR을 이용하여 돌돔에서 COX-2 유전자의 전체 염기서열을 밝혔으며, 그 결과 rbCOX-2 (rock bream COX-2)유전자 cDNA의 전체 길이는 2655 bp 였으며, 609개의 아미노산으로 구성되어있었다. rbCOX-2 유전자의 genomic organization은 9개의 intron과 10개의 exon으로 구성되어 있었다. 또한 본 연구에서는 돌돔에 megalocytivirus의 인위감염 시 COX-2 유전자의 발현을 조사하였다. LPS 접종 시 rbCOX-2 유전자는 접종 1일 후 대조구와 비교하여 13.10배 증가하여 최고 발현을 보였으나, megalocytivirus 접종 시 대조구와의 비교에서 유의적인 발현을 확인할 수 없었다. 돌돔에서 COX-2 유전자의 염기서열의 분석과 발현 분석은 바이러스 감염 시 어류의 방어기작을 이해하는데 도움이 될 것이며, 바이러스 백신개발 및 치료제 개발의 기초자료로 활용될 것이다.

• 과학기술학연구
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• 제18권2호
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• pp.181-232
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• 2018

한국의 엔지니어를 연구하려면 불가피하게 엔지니어가 누구인지에 대해 먼저 정의할 필요가 있다는 데서 이 연구는 출발한다. 엔지니어라는 영어 표현 속에는 한국을 비롯하여 동아시아 국가의 기술 관련 행위자들이 갖는 다양한 이름 뿐 아니라 그들의 역사가 숨 쉬고 있다. 그 이름들이 갖는 의미와 지위는 서로 다르다. 엔지니어가 누구인가에 관한 탐색은 동시에 한국 엔지니어의 역사를 이해하는 일이기도 하다. 이에 본 논문은 한국 엔지니어를 연구하는 데 필요한 질문들을 체계적으로 제시하고 그것을 다룰 적합한 연구방법론을 모색하는데 관심을 갖는다. 우리는 지금까지 이러한 연구에 요구되는 학문 간 토론과 접촉면을 만들고 확장하는데 충분한 노력을 기울이지 못했다. 비록 문제계가 다른 개념들을 결합시켜야 하는 이론적 난점이 존재하지만 이 연구는 사회과학 분야에서 이루어진 선행 연구들을 기반으로 어떻게 엔지니어 연구를 수행할 수 있을지, 한국 엔지니어의 형성 과정을 분석하기 위해 제기되어야 할 질문은 무엇이며 그에 적합한 개념과 방법론은 무엇인지를 논의하고자 한다. 연구 결과로서는 계보학, 개념사, 행위자-연결망과 기술-국가 개념 등이 한국 엔지니어의 역사를 분석하고 설명하는데 매우 효과적일 수 있음을 주장할 것이다.

• KDI Journal of Economic Policy
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• 제26권2호
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• pp.249-280
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• 2004

1990년대의 한국경제는 여러 가지 극적인 변화를 경험하였다. 가장 극적이었던 경제위기와 그로부터의 회복에 가려지기 쉽지만, 우리 경제가 겪은 가장 중요한 변화 중의 하나는 총요소생산성이 산업성장의 주요 동력으로 자리매김한 것이다. 본 연구는 이 기간 동안의 산업성장동력의 변화가 무역성과에 미친 영향을 특히 한국-중국-일본의 구도 속에서 분석한다. 무역성과의 분석을 위해서는 산업별 수출성과, CEPII 현시비교우위지수, 경합도 등이 사용되었다. 분석결과, 한국이 거의 모든 산업, 특히 노동집약적인 분야에서는 중국의 추격을 허용한 반면, 자본집약적이거나 기술집약적인 부문, 즉 총요소생산성의 증가가 성장을 주도한 산업에서는 일본을 극히 제한된 부문에서만 따라 잡는 것으로 나타나, 저간에 회자되는 'nut-cracking'의 우려가 1990년대부터 발생하였음을 보이고, 이로부터 벗어나는 것이 더 이상 미룰 수 없는 심각하고 시급한 과제임을 역설한다.

• Asian Journal of Atmospheric Environment
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• 제6권4호
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• pp.288-303
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• 2012

In this study, real time measurements of particle number size distribution in urban Gwangju, coastal Taean, and industrial Yeosu in Korea were conducted in 2008 to understand the occurrence of ultrafine particle (UFP) (<100 nm) events, the variation of its concentration among different sampling sites, and UFP formation pathways. Also, to investigate seasonal and long-term variation of the UFP number concentration, data were collected for the period of 5 years (2007, 2008, 2010, 2011, and 2012) in urban Gwangju. Photochemical and combustion events were found to be responsible for the formation of UFP in the urban Gwangju site, whereas only photochemical event led to the formation of UFP in the coastal Taean site. The highest UFP concentration was found in industrial Yeosu (the average UFP number fractions were 79, 59 and 58% in Yeosu, Gwangju, and Taean, respectively), suggesting that high amount of gas pollutants (e.g., $NO_2$, $SO_2$, and volatile organic carbon (VOC)) emitted from industries and their photochemical reaction contributed for the elevated UFP concentration in the industrial Yeosu site. The UFP fraction also showed a seasonal variation with the peak value in spring (61.5, 54.5, 50.5, and 40.7% in spring, fall, summer, and winter, respectively) at urban Gwangju. Annual average UFP number concentrations in urban Gwangju were $5.53{\times}10^3\;cm^{-3}$, $4.68{\times}10^3\;cm^{-3}$, $5.32{\times}10^3\;cm^{-3}$, $3.99{\times}10^3\;cm^{-3}$, and $2.16{\times}10^3\;cm^{-3}$ in the year 2007, 2008, 2010, 2011, and 2012, respectively. Comparison of the annual average UFP number concentration with urban sites in other countries showed that the UFP concentrations of the Korean sites were lower than those in other urban cities, probably due to lower source strength in the current site. TEM/EDS analysis for the size-selected UFPs showed that the UFPs were classified into various types having different chemical species. Carbonaceous particles were observed in both combustion (soot and organics) and photochemical events (sulfate and organics). In the photochemical event, an internal mixture of organic species and ammonium sulfate/bisulfate was identified. Also, internal mixtures of aged Na-rich and organic species, aged Ca-rich particles, and doughnut shaped K-containing particles with elemental composition of a strong C with minor O, S, and K-likely to be originated from biomass burning nearby agricultural area, were observed. In addition, fly ash particles were also observed in the combustion event, not in the photochemical event.

• Asian Journal of Atmospheric Environment
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• 제9권2호
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• pp.114-127
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• 2015

In the Mexico City Metropolitan Area (MCMA), ozone ($O_3$) concentration is still higher than in other urban areas in developed countries. In order to reveal the current state of photochemical air pollution and to provide data for validation of chemical transport models, vertical profiles of meteorological parameters and ozone concentrations were measured by ozonesonde in two field campaigns: the first one, during the change of season from wet to dry-cold (November 2011) and the second during the dry-warm season (March 2012). Unlike previous similar field campaigns, ozonesonde was launched twice daily. The observation data were used to analyze the production and distribution of ozone in the convective boundary layer. The observation days covered a wide range of meteorological conditions, and various profiles were obtained. The evolution of the mixing layer (ML) height was analyzed, revealing that ML evolution was faster during daytime in March 2012 than in November 2011. On a day in November 2011, the early-morning strong wind and the resulting vertical mixing was observed to have brought the high-ozone-concentration air-mass to the ground and caused relatively high surface ozone concentration in the morning. The amount of produced ozone in the MCMA was estimated by taking the difference between the two profiles on each day. In addition to the well-known positive correlation between daily maximum temperature and ozone production, effect of the ML height and wind stagnation was identified for a day in March 2012 when the maximum ground-level ozone concentration was observed during the two field campaigns. The relatively low ventilation coefficient in the morning and the relatively high value in the afternoon on this day implied efficient accumulation of the $O_3$ precursors and rapid production of $O_3$ in the ML.

• 시큐리티연구
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• 제37호
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• pp.29-50
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• 2013

최근 동북아시아 각국은 천안함 피격, 연평도 포격 등 북한의 대남 도발, 북한의 장거리미사일 발사와 핵실험 등 군사적 위협, 센카쿠 열도(댜오위다오)를 둘러싼 중국과 일본 간의 충돌 등 심각한 안보 위기를 맞고 있다. 특히 한국과 일본은 각기 2013년 2월 박근혜 정부, 2012년 12월 제2차 아베 신조(안부보삼(安部普三)) 내각 등 새로운 정권 출범을 맞아, 공히 주요 선거공약으로 제시한바 있는 위기관리 및 안보 정책의 정비 강화를 시도하고 있다. 그 핵심 중 하나는 최근 미국의 모델을 참조로 한 안보 및 위기관리 정책에서의 NSC 또는 그 유사 기구의 기능 확대 및 강화이다. 해당 각국의 NSC 조직은 각기 다른 특성을 가지고 있으나, 현 정치 안보 상황에서 안보 및 위기관리 정책의 컨트롤 타워로서 주된 역할을 담당할 수 있는 잠재성이 있다고 볼 수 있다. 따라서 이 논문에서는 한국, 미국, 일본 등 3개 국가 정부의 NSC 기구를 그 조직, 기능, 역사를 중심으로 비교 분석하고, 최근 이 3개 국가들, 특히 대한민국이 직면한 정치 안보 상황에 비추어 시사점을 도출해 보고자 한다.

• 생약학회지
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• 제46권1호
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• pp.44-51
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• 2015

The objective of this study is to characterize a toxicity of Polygalae Radix (PR) in F344 rats and to find a dose levels for the 13 weeks toxicity study. PR is well known as medicinal herb in many Asian countries for treatment of expectorant, tonic, tranquillizer, antipsychotic agent and functional diet for improving memory. However, there is insufficient background information on toxicological evaluation of PR extract to support its safe use. Therefore, we conducted toxicological evaluation of this drug in compliance with OECD and KFDA guideline in this study. The extract of PR was administered orally to F344 rats at dose levels of 0, 500, 1000, 2000, 3500 and 5000 mg/kg/day for 2 weeks. Each group was composed to five male and five female rats. In the result, there were no treatment PR-related adverse changes in food consumption, hematology, clinical chemistry, urinalysis, gross finding at necropsy, organ weight examination. Four males at 5000 mg/kg/day were found dead during the treatment period. These animals showed salivation. The cause of death is still under investigation. The animals treated at 500, 1000, 2000, 3500 and 5000 mg/kg/day showed salivation and all animals at 5000 mg/kg/day exhibited lower body weight and cumulative weight gain in compared to those of control animals. Therefore, we recommend that a dose group of 3500 mg/kg/day is a highest treatment group in 13-week exposure study.

• 한국식품영양학회지
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• 제26권3호
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• pp.485-491
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• 2013

본 연구에서는 피로 회복 또는 원기 회복에 효능이 있는 것으로 알려진 홍경천과 홍삼을 이용하여 홍경천-홍삼 복합 발효물의 산화적 손상 억제 효과를 평가하고자 $H_2O_2$로 산화적 스트레스를 유도시킨 C2C12 근육세포에 홍경천-홍삼 복합 발효물의 처리한 후, 세포의 morphology, cell viability 및 항산화 효소들의 유전자 발현 양상을 비교, 분석하였다. 홍경천-홍삼 복합 발효물은 C2C12 근육세포의 cell viability를 유의적으로 증가시켰으며, Cu/Zn-SOD, Mn-SOD 및 GPx 등과 같은 세포내 항산화 효소의 발현을 증가시킬 뿐만 아니라, 근육세포 분화의 주요 전사인자인 Myo D의 발현 또한 증가시키는 것으로 나타났다. 이상의 결과로, 홍경천-홍삼 복합 발효물은 세포내 항산화 효소 시스템을 증가시켜 외부로부터의 산화적 손상에 대한 방어효능을 갖는 것으로 나타났으며, 향후 in vivo 시스템 이용한 추가적인 연구가 수행된다면, 홍경천-홍삼 복합 발효물을 이용한 항피로 건강기능식품의 소재개발이 가능할 것으로 판단된다.

• Journal of Community Nutrition
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• 제2권1호
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• pp.62-70
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• 2000

Nutrition education should be an important component of ongoing health promotion for older adults and their caregivers. This is because prevention through sound nutrition and food hygiene practices and regular excercise is the most cost-effective way to reduce risks for and deal with their major health problems. nutritions education services should effectively promote optimum intake and successful self-care. Unfortunately, however relative to other vulnerable groups, nutrition education for older adults has not been systematically developed or evaluated. Usually oder adults care a lot about their health, so this should be a relatively easy group to teach - but their increasing numbers, longevity and great diversity with respect to health, physical, and economic status and educational level present challenges. Some older adults may not perceive they would benefit from nutrition education, so interesting and motivating them is a challenge. The food and nutrition knowledge of older people has been acquired through a lifetime of experience. For most older adults in the Asian region, their sources are restricted by their restricted education, so that their major sources of information have been informal sources, such as television, radio, friends, family, and perhaps newspapers and magazines if they are literature. Nonetheless, dietary advice for older people should build on their existing knowledge and ingrained values. It should provide information useful in daily food selection, and focus on food, not nutrients - the same foods and groups considered appropriate for younger people, with consistent messages as given throughout the population. Attention must also be paid to discovering learning styles in older people. When we teach in schools, the young students are a captive audience resigned to their learning role. Learning by an older adult, however, reflects an effort to meet his or her perceived needs. Therefore, nutrition education should be a positive experience in a non-threatening environment, relaxed and non-competitive, and perhaps even social environment. The messages also need to be practical and achievable. A needs assessment is essential, because our ability to provide the most effective nutrition education will depend on our success in matching the needs, both perceived and unperceived. of this vulnerable group. Therefore, go to the potential older learners to assess their interest and preferences. Nutrition education activities for older adults are widespread, but few have been evaluated. Evaluation is therefore also recommended, particularly when new methods are used. Tips from other countries for giving successful nutrition education will be given, including some examples of applications as attempted in Hong Kong. Research needs will also be described. In conclusion, successful nutrition education for older adults depends on positive needs-based messages. This is may be hard to do, as few good examples are available to illustrate these principles.

• Molecular & Cellular Toxicology
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• 제1권4호
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• pp.224-229
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• 2005

Cirsii Japonici Herba (CJH) extract has been used for hundreds of years in Asian countries as a treatment for pollutant, radiation, and alcohol-induced liver damage. The reducing effect of CJH on hydrogen peroxide-induced reactive oxygen species (ROS) production, the main cause of cell damage or death, was evaluated using the HepG2 cell line. Cell survival was determined using MTS assay. The viability of cells treated with CJH was not significantly different from oxidative-stressed HepG2 cells. A dose-dependent inhibitory effect by CJH on ROS production was shown in oxidative-stressed cells using the $H_{2}DCFDA$ assay. To identify candidate genes responsible for the anti-oxidative effects of CJH on HepG2 cells, an oligonucleotide microarray analysis was performed. The expressions of five genes were decreased, whereas nineteen genes were up-regulated in CJH plus hydrogen peroxide treated cells, compared to only hydrogen peroxide treated cells. Among them, the expression of 5 genes was decreased in hydrogen peroxide treatment when compared to control. These genes are known to regulate cell survival and progression. On the other hand, it was shown that its main compounds were not a sylimarin or its analogs. The list of differentially expressed genes may provide further insight on the action and mechanism behind the anti-oxidative effects of Cirsii Japonici Herba.