• Journal of Veterinary Science
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• v.23 no.4
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• pp.55.1-55.12
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• 2022

Background: ASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection. Objective: To identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays. Method: We used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide. Results: The results of our prediction revealed that the possible antigen epitope regions were A23-29, A36-45, A72-94, A114-120, A124-130, and A137-150. The indirect ELISA showed that the peptides A23-29, A36-45, A72-94, A114-120, and A137-150 have good antigenicity. Moreover, the A36-45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44. Conclusions: Our study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.

• The Plant Pathology Journal
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• v.33 no.3
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• pp.288-295
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• 2017

Fig mosaic disease (FMD) is a viral disease that spreads in all Tunisian fig (Ficus carica L.) orchards. RT-PCR technique was applied to leaf samples of 29 fig accessions of 15 fig varieties from the fig germplasm collection of High Agronomic Institute (I.S.A) of ChattMariem, to detect viruses associated to FMD. Analysis results show that 65.5% of the accessions (19/29) and 80.0% (12/15) of the fig varieties are infected by FMD-associated viruses. From all fig accessions, 41.4% of them are with single infection (one virus) and 24.1% are with multi-infections (2 virus and more). Viruses infecting fig leaf samples are Fig mosaic virus (FMV) (20.7%), Fig milde-mottle-associated virus (FMMaV) (17.25%), Fig fleck associated virus (FFkaV) (3.45%), and Fig cryptic virus (FCV) (55.17%). A reliable protocol for FCV and FMMaV elimination from 4 local fig varieties Zidi (ZDI), Soltani (SNI), Bither Abiadh (BA), and Assafri (ASF) via in vitro culture of 3 meristem sizes was established and optimized. With this protocol, global sanitation rates of 79.46%, 65.55%, 68.75%, and 70.83% respectively for ZDI, SNI, BA, and ASF are achieved. For all sanitized varieties, the effectiveness of meristem culture for the elimination of FCV and FMMaV viruses was related to meristem size. Meristem size 0.5 mm provides the highest sanitation rates ranging from 70% to 90%.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39A no.5
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• pp.258-270
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• 2014

In this paper, we investigate channel capacity of two kinds of uplink OFDMA (Orthogonal Frequency Division Multiple Access) schemes, i.e. ZCZ (Zero Correlation Zone) code time-spread OFDMA and sparse SC-FDMA (Single Carrier Frequency Division Mmultiple Access) robust to access timing offset (TO) among multiple users. In order to reflect the practical condition, we consider not only access TO among multiple users but also peak to average power ratio (PAPR) which is one of hot issues of uplink OFDMA. In the case with access TO among multiple users, the amplified signal of users by power control might affect a severe interference to signals of other users. Meanwhile, amplified signal by considering distance between user and base station might be distorted due to the limit of amplifier and thus the performance might degrade. In order to achieve the maximum channel capacity, we investigate the combinations of transmit power so called ASF (adaptive scaling factor) by numerical simulations. We check that the channel capacity of the case with ASF increases compared to the case with considering only distance i.e. ASF=1. From the simulation results, In the case of high signal to noise ratio (SNR), ZCZ code time-spread OFDMA achieves higher channel capacity compared to sparse block SC-FDMA. On the other hand, in the case of low SNR, the sparse block SC-FDMA achieves better performance compared to ZCZ time-spread OFDMA.

• Journal of Korea Society of Digital Industry and Information Management
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• v.18 no.1
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• pp.25-35
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• 2022

The SAP(salt-and-pepper) noise changes the pixel value to the maximum and minimum values of the dynamic region of the pixel. For this reason, unlike white Gaussian noise, SAP noise can predict the ratio of noise relatively easily. Because the condition of the neighboring pixels that can be referenced changes according to the noise ratio, it is necessary to apply different noise reduction methods according to the noise ratio. This paper proposes an adaptive switching filtering algorithm which can eliminates the SAP noise. It consists of two phases. It first detects the location of the SAP noise and calculates the noise ratio. After that, the image is reconstructed using different methods depending on which of the three sections the calculated noise ratio belongs to. As a result of the experiment, the proposed method showed superior objective and subjective image quality compared to the previous methods such as MF, AFSWMF, NAMF and RWMF.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.47 no.1
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• pp.15-21
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• 2010

This paper proposes the multimedia fingerprinting code insertion algorithm when video content is distributed in P2P environment, and designs the collusion codebook SRP(Small RISC Processor) embedded system for the collusion attack prevention. In the implemented system, it is detecting the fingerprinting code inserted in the video content of the client user in which it requests an upload to the web server and in which if it is certified content then transmitted to the streaming server then the implemented system allowed to distribute in P2P network. On the contrary, if it detects the collusion code, than the implemented system blocks to transmit the video content to the streaming server and discontinues to distribute in P2P network. And also it traces the colluders who generate the collusion code and participates in the collusion attack. The collusion code of the averaging attack is generated with 10% of BIBD code v. Based on the generated collusion code, the codebook is designed. As a result, when the insert quantity of the fingerprinting code is 0.15% upper in bitplane 0~3 of the Y(luminance) element of I-frame at the video compression of ASF for a streaming service and MP4 for an offline offer of video content, the correlation coefficient of the inserted original code and the detected code is above 0.15. At the correlation coefficient is above 0.1 then the detection ratio of the collusion code is 38%, and is above 0.2 then the trace ratio of the colluder is 20%.

• 한국HCI학회:학술대회논문집
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• 2008.02b
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• pp.600-607
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• 2008

메타버스 개발을 위한 세계 각국의 행보가 빨라지고 있다. 미국의 <세컨드라이프>를 시작으로 MS와 구글이 차세대 메타버스 개발을 서두르고 있고, 일본에서도 , , 등이 이미 출시되었거나 출시를 앞두고 있다. 이에 발맞춰 메타버스에 관한 연구도 한창인데, 2006년 5월 미국 캘리포니아에서 열린 제1회 메타버스 로드맵 서alt(Metaverse Roadmap Summit)이 대표적이다. 미국미래학협회(ASF)주최로 열린 이 행사에는 미국의 저명한 IT전문가, 게임제작자들이 대거 참가했고, 조직위측은 이날 햇사에서 진행된 수십 시간 분량의 토론을 인터넷에 고시해서 1년동안 각계각층의 의견을 수렴했다. 그리고 1년 후인 2007년 6월 <메타버스 로드맵 3D웹으로 가는길>을 발표됐다. 뿐만 아니라 비슷한 시기에 일본의 <노무라연구소>에서도 IT로드맵을 발표하고 메타버스의 발전전망을 제시했다. 이에 반해 세계최고의 IT기술력을 자랑하는 국내에서는 메타버스에 관한 개발과 연구가 매우 미흡한 실정이다. 이에 본 고에서는 미국의 <메타버스 로드맵>과 일본의 을 중심으로 메타버스의 개념과 발전전망을 살펴보고, 현존하는 가상세계 중 메타버스 개념에 가장 근접했다고 평가받는 린든랩의 <세컨드라이프>의 의의와 한계를 짚어보았다. 또한 제2, 제3의 <세컨드라이프>를 노리고 있는 세계 각 국의 메타버스 개발동향을 분석하고 이를 통해서 차세대 메타버스 개발의 성공조건으로서 풍부한 콘텐츠 제공과 SNS기능의 강화, 미러월드의 발전가능성등에 주목해야 함을 알 수 있었다. 이러한 연구결과는 90년대 온라인게임의 성공 이후 장기간 침체 현상을 보이고 있는 우리의 가상세계 개발연구에 유의미한 시사점을 지닌다고 하겠다.

• The Journal of Natural Sciences
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• v.8 no.1
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• pp.23-26
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• 1995

Operating characteristics of a $Cr^3+$:LiASF laser pumped by 640 nm and pulsed DCM dye laser are observed. The laser consists of a 93% reflectivity of half mirror and a total reflector, and the length between two mirrors is 3cm. Maximum efficiency is 10% for 22 mJ of pumping energy and the maximum slope efficiency is 19%.

• Transactions of the Korean Society of Automotive Engineers
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• v.11 no.4
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• pp.68-78
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• 2003

Recently, low speed vehicle (LSV) is beginning to appear for various usages. The body of the LSV is usually made of the aluminum space frame (ASF) type rather than the monocoque or unitary construction type. A pa.1 of the reason is that it is easier to reduce mass efficiently while the required stiffness and strength are maintained. A design flow for LSV is proposed. Design specifications for structural performances of LSV do not exist yet. Therefore, they are defined through a comparative study with general passenger automobiles. An optimization problem is formulated by the defined specifications. At first, one pillar which has an important role in structural performances is selected and the reinforcements of the pillar are determined from topology optimization to maximize the stiffness. At second, the thicknesses of cross sections are determined to minimize the mass of the body while design specifications are satisfied. The optimum solution is compared with an existing design. The optimization process has been performed using a commercial optimization software system, GENESIS 7.0.

• Korean Journal of Pharmacognosy
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• v.44 no.3
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• pp.242-252
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• 2013

This study was evaluated to biological activity of breeding lines from Acantopanax( A. sessiliflorus: ASF, A. koreanum: AKN, A. chiicanensis: ACS, A. senticosus: AST) and parts(root, stem, fruit, and leaf) and various extracted solvents( 100% water, 100% EtOH, 50% EtOH). Total polyphenol content of AKN root in 100% water extracts was high detected 464.46 mg/100 g. Total flavonoid content in the leaf was significantly higher than in other parts. The content of total sugars was high in the 50% EtOH extracts and fruit. The major free amino acids were arginine in all extracts. The content of arginine was detected in the root of AKN(1.807 mg/100 mg). Contents of eleutheroside B, E were high detected in 100% water extracts. Antioxidative capacity in the leaves of AKN was the higher than other extracts($EC_{50}=84.8{\mu}g/mL$). The results would be useful for understanding of the physiological properties of AKN extracts.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.1
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• pp.80-96
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• 2022

Nowadays, artificial intelligence promotes the rapid development of skin cancer detection technology, and the federated skin cancer detection model (FSDM) and dual generative adversarial network model (DGANM) solves the fragmentation and privacy of data to a certain extent. To overcome the problem that the many-objective evolutionary algorithm (MaOEA) cannot guarantee the convergence and diversity of the population when solving the above models, a many-objective evolutionary algorithm based on integrated strategy (MaOEA-IS) is proposed. First, the idea of federated learning is introduced into population mutation, the new parents are generated through sub-populations employs different mating selection operators. Then, the distance between each solution to the ideal point (SID) and the Achievement Scalarizing Function (ASF) value of each solution are considered comprehensively for environment selection, meanwhile, the elimination mechanism is used to carry out the select offspring operation. Eventually, the FSDM and DGANM are solved through MaOEA-IS. The experimental results show that the MaOEA-IS has better convergence and diversity, and it has superior performance in solving the FSDM and DGANM. The proposed MaOEA-IS provides more reasonable solutions scheme for many scholars of skin cancer detection and promotes the progress of intelligent medicine.