• The Journal of Advanced Prosthodontics
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• v.6 no.1
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• pp.14-21
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• 2014

PURPOSE. The purpose of this study was to evaluate bone response to anodized titanium implants coated with the extract of black cohosh, Asarum Sieboldii, and pharbitis semen. MATERIALS AND METHODS. Forty anodized titanium implants were prepared as follows: group 1 was for control; group 2 were implants soaked in a solution containing triterpenoids extracted from black cohosh for 24 hours; group 3 were implants soaked in a solution containing extracts of black cohosh and Asarum Sieboldii for 24 hours; group 4 were implants soaked in a solution containing extracts of pharbitis semen for 24 hours. The implants from these groups were randomly and surgically implanted into the tibiae of ten rabbits. After 1, 2, and 4 weeks of healing, the nondecalcified ground sections were subjected to histological observation, and the percentage of bone-to-implant contact (BIC%) was calculated. RESULTS. All groups exhibited good bone healing with the bone tissue in direct contact with the surface of the implant. Group 2 ($52.44{\pm}10.98$, $25.54{\pm}5.56$) showed a significantly greater BIC% compared to that of group 3 ($45.34{\pm}5.00$, $22.24{\pm}2.20$) with respect to the four consecutive threads and total length, respectively. The BIC% of group 1 ($25.22{\pm}6.00$) was significantly greater than that of group 3 ($22.24{\pm}2.20$) only for total length. CONCLUSION. This study did not show any remarkable effects of the extract of black coshosh and the other natural products on osseointegration of anodized titanium implants as coating agents. Further studies about the application method of the natural products on to the surface of implants are required.

• Korean Journal of Plant Resources
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• v.7 no.2
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• pp.177-182
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• 1994

This study was carried out to investigate about cultivation and export of major medicinal crops in Korea. The results obtained were summarized as follows'Planted area, harvested area and pro-duction were increase very much, but yield was not increased almost to 1993 from 1988. In spiteof, cultivation area was increased, export was decreased to 1993 from 1988. In cultivation area, major medicinal crops were Angelica gigas, Platycodon grandiflorum, Astragalus membranaceus, Paeonia lactiflora and Codonopsis lanceolata in 1993. In amount of export, major medicinal cropswere Ginkgo biloba, Bupleurum falcatum, Cornus officinalis, Asarum sieboldii in 1993.

• Journal of Acupuncture Research
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• v.33 no.1
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• pp.23-36
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• 2016

Objectives : The aim of this study was to investigate the asthma-suppressive and immuno-regulatory effect of AHCR-HA (Root of Asarum sieboldii Miq. water extract) on OVA (ovalbumin)-induced asthma in mice. Methods : All C57BL/6 mice experimental groups, except the Normal and N-AHCR groups, were sensitized with OVA. The mice in the N-AHCR group and the OVA-AHCR group were treated with water extract of AHCR (1 %) by an oral administration, and the OVA-Saline group were treated with saline solution. Oral dosesof AHCR water extract and saline were administered for 8 weeks, three times a week. Results & Conclusion : The lung weight and total cells in the lungs of the OVA-AHCR group decreased significantly compared with those of the OVA-Saline group. Total leukocytes and eosinophils in BALF and photomicrographs of the OVA-AHCR group decreased significantly compared with those of the OVA-Saline group. The collagen accumulation in the lung sections of the OVA-AHCR group decreased significantly compared with those of the OVA-Saline group. The concentrations of IL-4, IL-5, IL-13 and IgE in BALF and the serum of the OVA-AHCR group decreased significantly compared with those of the OVA-Saline group. The mRNA expressions of $TNF-{\alpha}$, $IL-1{\beta}$, IL-4, IL-5 and IL-13 in the lungs of the OVA-AHCR group decreased significantly compared with those of the OVA-Saline group.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.3
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• pp.666-671
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• 2003

Dental caries are the most commonly occurring chronic diseases in the dental field. Because of increasing sugar consumption and extension of average human life, these diseases are widely found all over the world as the most typical cause for a person to lose a tooth. Therefore, the development of more effective, substantial and safe preventive agents against dental caries is strongly required. Streptococcus mutans is known as the causative bacterial playing the most important role informing plaque and it is being noticed as major causative bacteria of dental caries. The present study was designed to investigate the effect of Asarum sieboldii Miquel(Aristolochiaceae) extracts on the growth, acid production, adhesion, and insoluble glucan synthesis of Streptococcus mutans(S. mutans). Both methanol and aqueous extracts showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 100, 1,000 and 2,000 μg/ml compared to the control group(p<0.05 - p<0.01). The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 50% at the concentration of 10 μg/ml and complete inhibition was observed at the concentration of 2,000 μg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan from sucrose, methanol and aqueous extracts showed more than 70% inhibition over the concentration of 1,000 μg/ml. Hence, we conclude that Asarum sieboldii might be a candidate of anticaries agent.

• Journal of Conservation Science
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• v.33 no.4
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• pp.255-266
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• 2017

Chemically derived pesticides have been used to prevent biological damage to domestic cultural property. However, their use is gradually being restricted due to the harmful effects on the human body and environment. Therefore, there is a growing interest in the search for new antifungal biopharmaceuticals whose safety has been confirmed by toxicity evaluation through animal experiments. This paper presents methods of toxicity evaluation of natural biocides using Sprague-Dawley rats and New Zealand White (NZW) rabbits. Safety of the natural biocide extract of Asarum sieboldii was evaluated using single-dose oral and dermal toxicity tests in Sprague-Dawley rats, and eye and skin irritation tests in NZW rabbits. The extract has proven antimicrobial and insecticidal activities against wood-rotting fungi and termites. After single oral administration to rats, the $LD_{50}$ values were determined to be over 4,000 and 2,000 mg/kg for males and females, respectively. After single dermal administration to rats, the $LD_{50}$ values exceeded 10,000 mg/kg for both males and females. The extract was identified to be non-irritant to the rabbit eye, and only slightly irritant to the rabbit skin. In this study, we confirmed the safety of the A sieboldii extract through animal testing. Due to the harmfulness of humidifier disinfectants, focus is on the safety of chemical pesticides, and toxicity evaluation is suggested as the basic method for hazard evaluation.

• 보존과학연구
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• s.29
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• pp.125-136
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• 2008

To investigate bioactive materials for development of natural conservative agent on organic cultural heritage, methanol extracts from 17 medicinal plants were screened for antifungal and insecticidal activity against 6 wood rot fungi and adult of Lasioderma serricorne. Antifungal activity of extracts was tested by using paper disc soaking method against wood-rot fungi. Among these extracts, the most significant antimicrobial activity was observed from the extract of Coptis japonica at 5 mg/disc against all wood rot fungi tested. The insecticidal activity of extracts was examined by topical application against L. serricorne adults. The extracts from Asarum sieboldii gave 83.3 % mortality at $25{\mu}g/adult$ for 24hr. From these result, the methanol extract from C. japonica and A. sieboldii described could be useful for conservation of organic cultural heritage against biological deterioration by wood rot fungi and L. serricorne.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.344-354
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• 2019

In seven species of Korean Asarum, 44 qualitative and 28 quantitative characters were reviewed taxonomically. Among 44 qualitative characters observed in vegetative and reproductive organs, the states of 27 characters were common in all the seven species. Among 28 quantitative characters, five characters have the same values in all the seven species; the number of cataphyll (2-4), leaf (2), calyx lobe (5), stamen (12) and pistil (6). White spots on adaxial surface of leaf (effectively) distinguished A. maculatum and A. versicolor from the other species. Many long soft hairs on abaxial surface of leaf distinguished A. mandshuricum from other species with short hairs. The glandular hairs on inner surface of calyx tube were divided into 2 types, reflecting the taxonomic relationships between the species: Type 1 has non-stalked glandular hairs (A. maculatum, A. sieboldii and A. sp.), and Type 2 has stalked glandular hairs (A. patens, A. versicolor, A. mandshuricum and A. misandrum). The length, shape, colour of calyx lobe and the extent to which the calyx lobe recurves, as well as the diameter and colour of calyx tube throat, were confirmed to be taxonomically valuable. In the case of glandular hair density on the surface of calyx lobe, A. patens with low density and A. sp. with high density were clearly distinct from all the other species with intermediate hair density. Meanwhile, the stylar protuberance length of A. patens (2.9-3.3 mm) was strikingly different from other species (1.4-2.5 mm). Additionally, further examination of the identities of A. yeonbyeonense and A. yeonbyeonense var. viridiluteolum is required.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1992.05a
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• pp.46-46
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• 1992

항궤양약 및 진경약의 효능을 검색할 수 있는 방법을 추구하는데 목적이 있다. 항궤양약의 약효는 Shay궤양, 수침 Stress궤양, indomethacin, histamine 및 prednisolone으로 유발된 궤양에 대하여 실시하였고 진경약의 효능은 흰쥐 fundus 절편을 이용한 in vitro 방법으로 실시하였다. 그 결과 항궤양약 효능시험은 shay 궤양에 있어서 omeprazole은 10mg/kg (i.d.)의 용량에서 유의성 있는 항궤양 효과를 나타내었으며, 수침 stress궤양에서 famotidine은 0.3mg/kg(p.o.), indomethacin 궤양에서 famotidine은 3mg/kg(p.o.), histamine궤양에서 cimetidine 은 50mg/kg(p.o.), prednisolone궤양에서 cimetidine은 1mg/kg(p.o.)의 용량에서 유의성 있는 효과를 나타내었다. 또한 진경약의 약효검색에 관하여는 세신(Asarum sieboldii)의 MeOH엑스가 흰쥐 위절편(fundus) 표본에서 비상경적 진정 작용이 있음을 확인하였고, 그 작용강도는 papaverine의 1/50에 해당되나 이를 분획하였을 경우에 hexane 분획에서 papaverine의 1/2에 해당하는 강도가 있다는 결과를 얻었다.

• The Korean Journal of Pesticide Science
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• v.8 no.3
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• pp.220-230
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• 2004

This study was conducted of Korean native plants to screen herbicidal activity which could be used for the development of new natural herbicides. Ninety-eight plants were collected from Wan Island, Chollanamdo in Korea and their methanol extracts were obtained. Herbicidal activities of the methanol extracts were determined by seed bioassay using canola (Brassica napus L.) seedlings. Among ninety-eight species, twenty plants were highly herbicidal ($GR_{50}<1,000\;{\mu}g\;g^{-1}$): Abies holophylla MAXIM., Ailanthus altissima (MILL.) SWINGLE, Anthemis nobilis L., Aralia elata SEEM., Artemisia iwayomogi KITAMURA, Asarum sieboldii MIQ., Brassica campestris subsp. napus var. nippo-oleifera MAKINO, Clematis terniflora DC., Crataegus scabrida SARG., Gnaphalium affine D. DON, Jasminum nudiflorum LINDL., Kalopanax pictus (THUNE.) NAKAI, Machilus japonica S. et Z., Myrica rubra S. et Z., Osmunda japonica THUNB., Phytolacca esculenta V. Houtte, Platanus occidentalis L., Quisqualis indica L., Rubus hirsutus THUNB., Yucca smalliana FERN. Fifty plants were shown moderate herbicidal activity $(1,000\;{\mu}g\;g^{-1}, however, twenty-eight plants were not shown any herbicidal activity.

• The Korea Journal of Herbology
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• v.22 no.1
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• pp.49-52
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• 2007

Objectives: Asari Herba comes from the dried perennial herbaceous plant, Asarum sieboldii $M_{IQ}$., A. heterotropides $F_R$. SCHMIDT var. mandshuricum($M_{AXIM}$) $K_{ITAG}$. and A sieboldii $M_{IQ}$ var. seoulense $N_{AKAI}$., family Aristolochiaceae. This medicine is pungent in flavor(味辛), warm in nature(性溫), mildly toxic and affects the lung, kidney, and heart meridians. It is able to repel wind dispel cold(祛風散寒), remove the obstruction of the nose(宣通鼻竅), relieve pain(止痛) and warm the lungs to remove the retention of fluids(溫肺化飮). In a clinic it is prescribed for exterior syndrome of wine-cold type(風寒表證) manifested as headache and general pain. It is indicated for headaches, toothaches, bisyndrome of wind-cold-dampness type(風寒濕痺), etc. For many generations medical books have warned doctors that the dosage of Asari Herba should not be excessive because the pungent flavor has the effect of dispersing exopathogens from superficies of the body. The principle, 'Asari Herba should not exceed Jeon(錢, Qian, 3.0 gram)' is still considered to be relevant. Methods : Extensive research of several ancient medical texts has lead to the following conclusion. Results : The statement 'Asari Herba should not exceed Jeon' is a dosage which is used to prepare single recipe and powder. Conclusion : The definition of Jeon is not 1 Jeon(3.0 gram), but 1 Jeonbi(錢匕, Qianbi, 1.0 gram), moreover, the usage, collection. and refinement process are not in accordance with the contents of the ancient medical texts. A deeper study of Asari Herba's dosage in the future is expected to have interesting results.