• 대한예방한의학회지
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• 제26권2호
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• pp.25-35
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• 2022

Objectives : This study aims to analyze the patents filed in the clinical diagnosis sector where technologies have been actively developed since the advent of the 4th industrial revolution. Methods : The analysis has been conducted in two ways - the period from 2016 to 2021 and the time points before and after COVID-19 - by visualizing based on the word cloud method. Results : Over two thirds of patents has been filed in the A61B sector (71.8%) and cure, sensor, self diagnosis, control, and breakdown have been observed in the period above. During the overall period (2016~2021), 'ultrasound'(7.5%), 'image'(5.1%), 'skin'(4.0%), 'treatment'(3.4%), and 'artificial intelligence(2.5%)' were the frequently patent applications technologies. In addition, 'ultrasound'(6.2%), 'image'(5.5%), 'skin'(4.0%), 'treatment' (3.7%), and 'portable'(1.7%) appeared most frequently before COVID-19 whereas 'ultrasound(5.5%)', 'artificial intelligence(4.2%)', 'diagnostic device'(1.9%), 'dimentia'(1.6%), and 'diagnostic kit'(1.4%) emerged the most after COVID-19. Conclusion : This study is meaningful in that it showed the technological development trend in the digital diagnosis sector and it was found that the Korean medicine field should contribute to this field more actively in the future.

• 대한화장품학회지
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• 제31권3호
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• pp.253-257
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• 2005

피부노화의 정도를 판정하기 위해 사용되는 주름측정법들은 객관성과 재현성의 확보가 중요한 요소이다. 최근의 경향은 주름의 형태나 깊이에 주는 영향을 최소화하기 위해 주름 측정시 피부에 직접 기계나 도구를 접촉하지 않고 측정하는 비접촉식 측정방법으로 빠르게 전환되고 있는 상황이다. 저자들은 주름측정 기술의 변천 과정을 간단히 살펴보고, 비접촉식 fringe projection 방식의 미세주름 측정기기인 PRIMOS를 중심으로 측정원리, 특징들을 접촉식 측정방법인 모사판을 이용한 방법과 비교하였다.

• Journal of Microbiology and Biotechnology
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• 제28권12호
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• pp.2121-2132
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• 2018

Abnormal melanin synthesis results in several hyperpigmentary disorders such as freckles, melanoderma, age spots, and other related conditions. In this study, we investigated the anti-melanogenic effects of an extract from the microalgae Chlamydomonas reinhardtii (CE) and potential mechanisms responsible for its inhibitory effect in B16F10, normal human epidermal melanocyte cells, and human skin-equivalent models. The CE extract showed significant dose-dependent inhibitory effects on ${\alpha}$-melanocyte-stimulating, hormone-induced melanin synthesis in cells. Additionally, the CE extract exhibited suppressive effects on the mRNA and protein expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. The CE extract also inhibited the phosphorylation of protein kinase A and extracellular signal-related kinase, which function as upstream regulators of melanogenesis. Using a three-dimensional, reconstructed pigmented epidermis model, the CE-mediated, anti-pigmentation effects were confirmed by Fontana-Masson staining and melanin content assays. Taken together, CE extract can be used as an anti-pigmentation agent.

• 식물병연구
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• 제14권3호
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• pp.205-209
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• 2008

배 만수 품종에서 발생되는 수확 후 과피 갈변 발생원인을 구명하기 위해 2년간 시험한 결과를 요약하면 다음과 같다. 배 만수 품종의 과피 갈변은 수확 후 선과시 발생되는 미세한 물리적 상처 부위에서 갈변이 발생되었다. 따라서 과피에 인위적 상처를 낸 후 품종간 과피 갈변 정도를 비교한 결과 신고 품종에 비해 만수 품종이 과피 갈변 발생정도가 심하여 품종간 차이를 나타내었다. 또한 만수 품종은 신고 품종에 비해 과피 조직이 얇았으며, 과피내황산화능이 높고 함량이 많을수록 과피 갈변 발생이 적었다. 결과적으로 만수 품종의 과피 갈변은 수확후 선과주에 발생하는 물리적인 미세상처 발생을 억제하기 위해 수확 후 봉지를 씌운채 선과하면 과피의 상처 발생감소로 갈변 발생이 현저히 억제되었다.

• Journal of Ginseng Research
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• 제42권4호
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• pp.512-523
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• 2018

Background: 20(S)-Protopanaxadiol (20S-PPD) is a fully deglycosylated ginsenoside metabolite and has potent dermal antiaging activity. However, because of its low aqueous solubility and large molecular size, a suitable formulation strategy is required to improve its solubility and skin permeability, thereby enhancing its skin deposition. Thus, we optimized microemulsion (ME)-based hydrogel (MEH) formulations for the topical delivery of 20S-PPD. Methods: MEs and MEHs were formulated and evaluated for their particle size distribution, morphology, drug loading capacity, and stability. Then, the deposition profiles of the selected 20S-PPD-loaded MEH formulation were studied using a hairless mouse skin model and Strat-M membrane as an artificial skin model. Results: A Carbopol-based MEH system of 20S-PPD was successfully prepared with a mean droplet size of 110 nm and narrow size distribution. The formulation was stable for 56 d, and its viscosity was high enough for its topical application. It significantly enhanced the in vitro and in vivo skin deposition of 20S-PPD with no influence on its systemic absorption in hairless mice. Notably, it was found that the Strat-M membrane provided skin deposition data well correlated to those obtained from the in vitro and in vivo mouse skin studies on 20S-PPD (correlation coefficient $r^2=0.929-0.947$). Conclusion: The MEH formulation developed in this study could serve as an effective topical delivery system for poorly soluble ginsenosides and their deglycosylated metabolites, including 20S-PPD.

• 한국안광학회지
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• 제11권2호
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• pp.99-107
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• 2006

본 연구의 목적은 기초화장품에 노출된 소프트콘택트렌즈를 착용하였을 때 나타나는 불편감의 원인을 밝히는 것이다. 소프트콘택트렌즈를 스킨로션, 에센스로션 또는 영양크림에 노출 후 다시 7일간 인공누액에 노출하여 부착된 단백질 양, 가시광선 투과도, 습윤성 및 곡률반경의 변화를 측정하였다. 소프트콘택트렌즈에 부착된 단백질 양은 기초화장품의 노출에 의해 약 3배 증가하였다. 또한 영양크림과 인공누액 모두에 노출된 소프트콘택트렌즈의 가시광선 투과도는 92.8%로 인공누액에만 노출된 소프트콘택트렌즈의 97.5%보다 4.7% 더 낮았다. 스킨로션, 에센스로션, 영양크림에 노출됨에 따라 렌즈의 접촉각은 각각 $7.1^{\circ}$, $7.0^{\circ}$, $5.7^{\circ}$가 증가하였으며, 소프트콘택트렌즈의 곡률반경은 각각, 3.2%, 5.1% 및 8.3% 더 감소하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.92-94
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• 2003

각질세포의 배양법이 개발 후 피부 결손 부위의 치료에 인체 각질세포를 배양하여 얻어진 표피를 이식하는 방법부터 세포부유물을 도포하는 방법들은 Trypsin 처리 과정을 거치면서배양된 세포의 부착능력을 가진 단백질이 손상되어 성공적인 피부 재생이 불가능하다. 본 실험에서는 이러한 효소처리의 단점을 보완하기 위해서 고분자 미립구에 세포를 효소처리 과정없이 동물실험을 한 결과 21일 후에는 대조군에 비해서 표피가 완벽하게 재생되었고 이식 후 세포의 부착면적을 늘리고 생착율을 높일 수 있었다. 이 연구에서 효소처리 없이 고분자 미립구에서 배양된 세포를 이용한 인공피부 재생이 효과적인 것을 보여주었다. 이러한 모델은 앞으로 화상이나 궤양으로 인한 피부 손실 부위 치료에 사용될 수 있을 것으로 생각된다.

• Archives of Reconstructive Microsurgery
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• 제10권2호
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• pp.163-168
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• 2001

The defects of the abdominal wall could be brought about either congenitally, for instances in such cases as omphalocele or gastroschisis, or by various acquired causes-trauma, excision of tumors, excision of burn scar, tissue necrosis caused by infection, hematoma after abdominal surgery, tissue necrosis after radiation therapy and so on. As for the techniques of the reconstruction of the abdominal wall defects, many authors have developed and reported diverse methods. To summarize, primary closure, skin graft, local skin flaps, various myocutaneous flaps, free flap, fascia graft, artificial mesh, tissue expansion, etc could be used in the reconstruction of the abdominal wall defects. The periumbilical perforator-based island skin flap has a many advantages such as no significant sacrifice of the rectus abdominis muscle, wide rotation arc, reliable blood flow of the perforator, short elevation time for flap, and for middle-aged, obese patients, the donor site may be the best from the cosmetic point of view. We used perforator-based island skin flap in 5 cases with reasonable result from March 1999 to May 2001. There were no significant complications and donor sites could be repaired primarily.

• The Korean Journal of Physiology and Pharmacology
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• 제18권3호
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• pp.249-254
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• 2014

The purpose of this study is to characterize the effects of KHG26792 (3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride), a potential skin whitening agent, on melanin synthesis and identify the underlying mechanism of action. Our data showed that KHG26792 significantly reduced melanin synthesis in a dose-dependent manner. Additionally, KHG26792 downregulated microphthalmia-associated transcription factor (MITF) and tyrosinase, the rate-limiting enzyme in melanogenesis, although tyrosinase was not inhibited directly. KHG26792 activated extracellular signal-regulated kinase (ERK), whereas an ERK pathway inhibitor, PD98059, rescued KHG26792-induced hypopigmentation. These results suggest that KHG26792 decreases melanin production via ERK activation. Moreover, the hypopigmentary effects of KHG26792 were confirmed in a pigmented skin equivalent model using Cervi cornus Colla (deer antler glue), in which the color of the pigmented artificial skin became lighter after treatment with KHG26792. In summary, our findings suggest that KHG26792 is a novel skin whitening agent.

• The Korean Journal of Physiology
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• 제7권1호
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• pp.41-47
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• 1973

$Na^{+}$ balance was studied in Rana temporaria, which hibenates in fresh water in the winter time. $Na^{+}$ uptake rate, skin $Na^{+}$ loss rate, urinary $Na^{+}$ loss rate and $Na^{+}-K^{+}$ adenosine triphosphatase(ATPase) were measured at two different temperatures $1{\sim}2^{\circ}C\;and\;20{\sim}24^{\circ}C$ respectively. The results obtained were as follows: 1. $Na^{+}$ uptake rates in the frog in an artificial Pond water (APW) were found to be $8.28{\pm}0.73\;and\;2.19{\pm}0.37\;{\mu}Eq/g/day\;at\;20{\sim}24^{\circ}C\;and\;1.0{\sim}2.5^{\circ}$ respectively. 2. $Na^{+}$ loss rate through the frog skin to APW were found to be $4.26{\pm}0.72\;and\;0.93{\pm}0.21\;{\mu}Eq/g/day$ at the same temperatures. 3. Mean rates of urinary $Na^{+}$ loss at $20{\sim}24^{\circ}C\;and\;3{\sim}4^{\circ}C$ were found to be $3.02{\pm}0.73\;and\;0.78{\pm}0.13\;{\mu}Eq/g/day$ respectively. 4. The activities of $Na^{+}-K^{+}$ activated ATPase of frog skin fragments were found to be $258{\pm}39.4\;and\;49.6{\pm}7.1\;{\mu}M\;Pi/g$ protein/hr at $24^{\circ}C\;and\;2^{\circ}C$ respectively. From the above results, it may be concluded that frogs can take up enough $Na^{+}$ through the skin from APW exceeding skin loss Plus urinary loss at $1{\sim}2^{\circ}C$. It is suggested that $Na^{+}$ transport across frog skin is closely related with $Na^+-K^+$ ATPase since $Q_{10}\;of\;Na^{+}$ uptake is much similar to that of the activities of $Na^{+}-K^{+}$ ATPase.