• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.59 no.6
• /
• pp.1136-1142
• /
• 2010

In this study, an efficient removal algorithm for specular reflections in a tooth color image is proposed to minimize the artefact interrupting color image segmentation. The pixel values of RGB color channels are initially reversed to emphasize the features in reflective regions, and then those regions are automatically detected by utilizing perceptron artificial neural network model and those prominent intensities are corrected by applying a smoothing spatial filter. After correcting specular reflection regions, multiple seeds in the tooth candidates are selected to find the regional minima and MCWA(Marker-Controlled Watershed Algorithm) is applied to delineate the individual tooth region in a CCD tooth color image. Therefore, the accuracy in segmentation for separating tooth regions can be drastically improved with removing specular reflections due to the illumination effect.

• Journal of Aquaculture
• /
• v.10 no.2
• /
• pp.199-211
• /
• 1997

Effects of light and temperature on the early growth of Hizikia fusiformis were investigated for establishment of artificial seed production. Young germlings were cultured for 70 days under different culutre conditions : light intensities (500, 1, 000, 2, 000, 4, 000, 8, 000 lux), photoperiods (8 : 16, 12 : 12, 16 : 8, (L : D)) and temperatue (10, 15, 20, 25, $30^{\circ}C$). Growth of the germlings was facilitated in 2, 000~4, 000 lux of light intensities, 16 : 8 (L:D) of photoperiods and 15~$20^{\circ}C$ of temperature. Culture conditions were revealed for the mass culture of artificial seeds of effective early growth in Hizikia cultivation.

• Journal of Korean Society of Forest Science
• /
• v.88 no.4
• /
• pp.485-489
• /
• 1999

The germination percentages of Quercus hybrid seeds produced in 1994 using stored pollens were low. The survival rate and height growth within 2 years differed according to crossing combinations. The survival rate and growth of seedlings from hybrid seeds produced in 1991 and 1993 were investigated. The survival rates of five-year-old and three-year-old seedlings were from 71 to 100% and from 33 to 100%, respectively. The survival rates differed according to cross combinations. The mortality of seedlings increased in proportion to the increase of percentages of dwarf seedlings. The growth of five-year-old seedlings, of which mother tree was Q. aliena, was the best. The growth of seedlings produced by artificial crossing tended to be worse than that of natural crossing.

• Korean Journal of Agricultural Science
• /
• v.15 no.1
• /
• pp.47-68
• /
• 1988

This study was conducted to define the optimal conditions for embryo growth during seed stratification and for breaking dormancy as well as seed germination of stratified ginseng seeds. The experiments were also carried out to detect some materials which were expected to induce seed dormancy in the ginseng seeds. The results summarized as follows; 1. The growth of embryo during seed stratification was significantly inhibited by the existence of endocarp. The fastest embryo growth was resulted at $15^{\circ}C$ and an estimated optimal temperature for embryo growth was about $18^{\circ}C$. 2. There was no significant difference between the embryo growth and germination ratio of ginseng seeds which were sown in seed bed at Aug-5 without seed stratification and that of artificial seed stratification. 3. Embryo growth and germination ratio was significantly inhibited by high temperature treatment at $30^{\circ}C$ for 24 hours or respiration stress by immersing seeds in water for 10 days or more. 4. When the seed stratification was started at $10^{\circ}C$, growth of embryo in the ginseng seeds were almost stopped. But, when the seeds were stratified first at $20^{\circ}C$ for 50 days and next at $10^{\circ}C$ for 50 days, the embryo growth was significantly promoted compared with the embryo growth in the seeds which were stratified at $20^{\circ}C$ for 100 days. 5. The successive embryo growth after seed stratification was significantly accelerated at $10^{\circ}C$ but the seeds chilled at $5^{\circ}C$ for 100 days were resulted in the highest germination ratio as well as the shortest days for germination. 6. The successive embryo growth during chilling treatment and seed germination were significantly inhibited by immersing seeds in water just before chilling treatment or during chilling treatment and by interruption of chilling treatment with raising temperature to $20^{\circ}C$ for 20 days during chilling treatment. 7. The germination ratio of ginseng seeds which finished chilling treatment was highest at $10^{\circ}C$ and 62.5% was the estimated soil moisture for the best germination of ginseng seeds. The ginseng seeds were found to require high amount of oxygen for germination. 8. Only water soluble material in homogenized ginseng seeds showed a significant inhibiting effect on the seed germination of sesame, millet and soybean. Water soluble material dissolved from undehisced ginseng seeds showed stronger inhibiting effect on the seedling growth of sesame than material from dehisced ginseng seeds. Extraction temperature did not influence the inhibiting effect of the material dissolved from ginseng seeds on the seedling growth of sesame. 9. Water soluble materials dissolved from the berry pulps, leaves, fresh roots and dried roots also showed a significant inhibiting effect on the seedling growth of sesame. 10. Water soluble materials dissolved from the ginseng seeds, leaves and fresh roots showed a significant inhibiting effect on the germination of true fungi and the growth of spawn but the growth of phytopathogenic bacteria was not. 11. Among the water soluble materials dissolved from ginseng seeds, the materials of low molecular weight less than 3,000 were resulted a significant inhibiting effect on the seedling growth of sesame and the materials of high molecular weight also showed an inhibiting effect.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.8 no.4
• /
• pp.81-90
• /
• 2005

This study aimed to address problems and suggest solutions in applying seed mixture design criterior of the slope revegetation works according to "The guidelines of Slope revegetation design and construction" proposed by the Ministry of Construction and Transportation. To do this, the planting thickness was set to be the same 2cm while the composition of seeds was made different, which was planted in artificial slopes and fields. The main results were summarized as follows. In the test, when applying the provisional standards of the plant mixtures proposed by the Ministry of Construction and Transportation, tree seeds and native plants were found poor coverage effect in the early stage in all test plots due to introduced grasses. This was because introduced winter grasses which grew well in the early stage, could grow densely, so it might hamper the growth of other plants. Therefore, it was found desirable that standards for seed mixing should be adjusted down by 10% and the ratio of introduced grasses in the mixture should be lowered to 25% while native plants should increase to 20-25%.

• The Plant Pathology Journal
• /
• v.22 no.1
• /
• pp.63-67
• /
• 2006

Apple scar skin, one of the most destructive diseases affecting apple, is caused by Apple scar skin viroid (ASSV d). Fruit dappling appeared on several cultivars in Korea and has been distributed to major cultivated areas since 2001. ASSVd was identified from infected fruits by using nucleic acid sequence-based amplification with electrochemiluminescence (NASBA-ECL). NASBA-ECL method was faster and hundredfold more sensitive than reverse transcription-polymerase chain reaction (RT-PCR) for ASSVd detection in apple leaves/ stems. ASSVd was rapidly transmitted to the entire tree in the second year after artificial inoculation. The ASSVd could be transmitted efficiently by using contaminated pruning scissors to both lignified stems (60 to $70\%$) and green shoots (20 to $40\%$) of apple tree and young plants. Dipping of contaminated scissors in $2\%$ sodium hypochlorite solution effectively prevented viroid transmission. In the ASSV d-infected fruits, the viroid was easily detected from fruit skin, seed coat, and embryo. Moreover, embryo and endosperm separately excised from the ASSVd-infected seeds were ASSVd positive in NASBA-ECL assay. Seedlings germinated from ASSVd-positive seeds showed $7.7\%$ infection rate., which indicated that ASSVd is seed-borne.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2008.11a
• /
• pp.399-399
• /
• 2008

Synthesis and characterization of ZnO structure such as nanowires, nanorods, nanotube, nanowall, etc. have been studied to multifunctional application such as optical, nanoscale electronic and chemical devices because it has a room-temperature wide band gap of 3.37eV, large exiton binding energy(60meV) and various properties. Various synthesis methods including chemical vapor deposition (CVD), physical vapor deposition, electrochemical deposition, micro-emulsion, and hydrothermal approach have been reported to fabricate various kinds of ZnO nanostructures. But some of these synthesis methods are expensive and difficult of mass production. Wet chemical method has several advantage such as simple process, mass production, low temperature process, and low cost. In the present work, ZnO nanorods are deposited on ITO/glass substrate by simple wet chemical method. The process is perfomed by two steps. One-step is deposition of ZnO seeds and two-step is growth of ZnO nanorods on substrates. In order to form ZnO seeds on substrates, mixture solution of Zn acetate and Methanol was prepared.(one-step) Seed layers were deposited for control of morpholgy of ZnO seed layers by spin coating process because ZnO seeds is deposited uniformly by centrifugal force of spin coating. The seed-deposited samples were pre-annealed for 30min at $180^{\circ}C$ to enhance adhesion and crystallinnity of ZnO seed layer on substrate. Vertically well-aligned ZnO nanorods were grown by the "dipping-and-holding" process of the substrates into the mixture solution consisting of the mixture solution of DI water, Zinc nitrate and hexamethylenetetramine for 4 hours at $90^{\circ}C$.(two-step) It was found that density and morphology of ZnO nanorods were controlled by manipulation of ZnO seeds through rpm of spin coating. The morphology, crystallinity, optical properties of the grown ZnO nanostructures were carried out by field-emission scanning electron microscopy, high-resolution electron microscopy, photoluminescence, respectively. We are convinced that this method is complementing problems of main techniques of existing reports.

• Research in Plant Disease
• /
• v.13 no.3
• /
• pp.145-151
• /
• 2007

Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max. (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.

• Journal of Life Science
• /
• v.21 no.7
• /
• pp.1060-1066
• /
• 2011

In order to investigate the variations on the ratio of mannose to galactose in the seeds of Phaseolus angularis, 17 local strains (Yangyang, Pyeongchang, Ganghwa, Pocheon, Geumsan, Seocheon, Jincheon, Danyang, Tongyeong, Sancheong, Gumneung, Wolseong, Wando, Gokseong, Okgu, Jangsu, Bukjeju), which are located from $33^{\circ}15'N$ to $38^{\circ}11'N$, were selected according to their latitudes and geographical distances. The seeds of these strains were collected and their contents of mannose and galactose were analyzed. Mannose contents in the seeds were variable, ranging from 17.071 mg/g at its highest (Jangsu) and 6.488 mg/g at its lowest (Geumsan). The contents of galactose also showed remarkable differences, ranging from 9.477 mg/g (Wolseong) to 19.877 mg/g (Jangsu). The local strains were classified into 3 variation types - coastal type I (Wando, Okgu, Bukjeju), the inland type (Jangsu, Weolseong, Danyang, Geumneung, Pyeongchang, Sancheong) and coastal type II (Ganghwa, Seocheon, Tongyeong, Jincheon), as well as 4 strange strains (Gokseong, Yangyang, Pocheon, Geumsan) according to the geographical climatic type and the ratio of mannose to galactose, which indicate the hardness of seeds in Leguminosae and ranged from 0.64 to 1.22. The variation types are very significant genecologically as evidence for microevolution related to natural and artificial selection in cultivated plants.

• Journal of Aquaculture
• /
• v.8 no.4
• /
• pp.307-316
• /
• 1995

Jicon scallop (Chlamys farreri), as one of new culture species, has been taken a growing interest in Korea recently. Artificial seeds are needed since seeds in natural waters are absolutely short. To develop the better methods for the artificial spawning and the rearing larvae on a large scale, experiments were carried out from July 1993 to May 1994. Mother shells were collected from the area near Tong-yeong and Geoje-do, the southern part of the Korean Penninsula. It was estimated that a period of 2-years was needed to be mother shells after the fertilization. Generally, spawning occourred intermittently from March to August and the maximum occurred in July. A best way for the artificial spawning was a chemical incentive, a 'serotonin injection' in parallel with the exposure and the temperature stimulation. Densities of the fertilized eggs for the normal development was from 30 to 40 per mililiter. It took 27-days from the fertilization to the settling larva averaging $155{\mu}m$ in size with water temperature 11.5\~13.0^{\circ}C$. Isochrysis galvana and Chaetoceros calcitrans with a density of $1,500\times10^4$ cells per ililiter were supplied for rearing the larvae. A proper density of larvae was 10 individuals per mililiter, and the survival rate of larvae from the flowing water system was much better than that of larvae from the still water.