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http://dx.doi.org/10.5423/RPD.2007.13.3.145

Detection of Xanthomonas axonopodis pv. glycines and Survey on Seed Contamination in Soybean Seeds Using PCR Assay  

Hong, Sung-Jun (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Hong, Yeon-Kyu (Applied Entomology Division, National Institute of Agricultural Science and Technology)
Lee, Bong-Choon (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Lim, Mi-Jung (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Yoon, Young-Nam (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Hwang, Jae-Bok (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Song, Seok-Bo (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Park, Sung-Tae (Plant Environment Division, Yeongnam Agricultural Research Institute, NICS, RDA)
Publication Information
Research in Plant Disease / v.13, no.3, 2007 , pp. 145-151 More about this Journal
Abstract
Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max. (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.
Keywords
PCR assay; Soybean seed; Xanthomonas axonopodis pv. glycines;
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1 Tegli, S., Sereni, A. and Surico, G. 2002. PCR-based assay for the detection of Curtobacterium flaccumfaciens pv. flaccunifaciens in bean seeds. Letters in Applied Microbiology 35: 331-337   DOI   ScienceOn
2 Hartman, G. L., Sinclair, J. B. and Rupe, J. C. 1999. Compendium of Soybean Diseases. APS PRESS Fourth edition: 6-7
3 Oh, C. S., Heu, S. G. and Choi, Y.-C. 1999. Sensitive and Pathovar-specific Detection of Xanthomonas axonopodis pv. glycines by DNA Hybridization and Polymerase Chain Reaction Analysis. Plant Pathol. J. 15(1): 57-61
4 Fett, W. F. 1979. Survival of Pseudomonas glycinea and Xanthomonas phaseoli var. sojensis in leaf debris and soybean seed in Brazil. Plant Dis. Rep. 63: 79-83
5 김용욱, 조준형. 2005. 국내 육성 콩 품종의 논 재배에 따른 생육 반응과 수량성. 한국작물학회지 50: 161-169   과학기술학회마을
6 Graham, J. H. 1953. Overwintering of three bacterial pathogens of soybean. Phytopathology 43: 189-192
7 Meng, X. Q., Umesh, K. C., Davis, R. M. and Gilbertson, R. L. 2004. Development of PCR-Based Assays for Detecting Xanthomonas campestris pv. carotae, the Carrot Bacterial Leaf Blight Pathogen, from Different Substrates. Plant Dis. 88: 1226-1234   DOI   ScienceOn
8 Srivastava, S. S. L. and Bais, B. S. 1987. Survival and virulence of Xanthomonas campestris pv. glycines causing leaf pustules of soybean. Indian J. Plant Pathol. 5: 22-23
9 Walcott, Ron. R. 2003. Detection of Seedbome Pathogens. HortTechnology 13: 40-47
10 Prosen, D., Hatziloukas, E., Schaad, N. W. and Panopoulos, N. J. 1993. Specific detection of Pseudomonas syringae pv. phaseolicola DNA in bean seed by polymerase chain reactionbased amplification of a phaseoltoxin gene region. Phytopathology 83: 965-970   DOI
11 Schaad, N. W., Jones, J. B. and Chun, W. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. APS Press, St. Paul, MN
12 Audy, P., Braat, C. E., Saindon, G, Huang, H. C. and Laroche, A. 1996. A rapid and sensitive PCR-based assay for concurrent detection of bacteria causing common and halo blights in bean seed. Phytopathology 86: 361-366   DOI
13 Berg, T., Tesoriero, L. and Hailstones, D. L. 2005. PCR-based detection of Xanthomonas campestris pathovars in Brassica seed. Plant Pathology 54: 416-427   DOI   ScienceOn
14 Prathuangwong, S., Khandej, K. and Goto, M. 1994. A semiselective medium for detecting Xanthomonas campestris pv. glycines in contaminated soybean seed. Soybean Feeds The World. B. Napompeth (ed.) Kasetsart University Press, Bangkok. 1: 197-201
15 Laviolette, F. A., Athow, K. L., Probst, A. H. and Wilcox, J. P. 1970. Effect of bacterial pustule on yield of soybeans. Crop Sci. 10: 150-151   DOI
16 Molouba, F., Guimier, C., Berthier, C., Guenard, M., Olivier, V., Baril, C. and Horvais, A. 2001. Detection of Bean Seed-Borne Pathogens by PCR. Proc. Int. Symp. on Molecular Markers 603-607
17 Hartwig, E. E. and Johnson, H. W. 1953. Effect of the bacterial pustule disease on yield and chemical composition of soybeans. Agron. J. 45: 22-23   DOI