• 약학회지
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• 제51권5호
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• pp.355-360
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• 2007

The essential oil fraction from the aerial parts of A. annua was analyzed by GC-MS. As the results, caryophyllene oxide (11.7%), caryophyllene (7.54%), camphor (7.32%), 1,8-cineol (4.98%), and borneol (3.99%) were confirmed as the main components of the oil fraction. The effects of this oil and its main components on antibiotic-susceptible and -resistant strains of Salmonella enteritidis and S. typhimurium were assessed. A. annua oil fraction significantly inhibited all strains of the two Salmonella species examined, with minimum inhibiting concentrations (MICs) ranging from 2.0 mg/ml to 8.0 mg/ml. Among the main components of the oil, borneol and camphor showed relatively strong inhibiting activity with MICs between 1.0 mg/ml and 4.0 mg/ml. The MICs of caryophyllene and caryophyllene oxide were higher than 16 mg/ml. The combination effects of the oils with kanamycin were evaluated using a checkerboard microtiter assay. Against S. typhimurium KCCM11862 and CCARM8009 strains, the oil fraction of A. annua, camphor, and 1,8-cineol exhibited significant synergistic with kanamycin with fractional inhibitory concentration (FIC) indices in the range of 0.085 to 0.375. In conclusion, a combination of kanamycin and A. annua oil or its main component, camphor, and cineol, may be useful for reducing the minimum effective dose of antibiotic required for the treatment of resistant S. typhimurium infections.

• 한국식품저장유통학회지
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• 제22권6호
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• pp.811-816
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• 2015

본 연구는 개똥쑥 분말(0 g, 30 g, 60 g, 90 g)을 반죽에 첨가하여 절편을 제조하고 절편의 영양성분, 색도, 아미노산 및 관능검사를 실시하여 개똥쑥 절편의 적합한 첨가비율과 최적 조건을 제시하고자 실시하였다. 일반성분의 경우 개똥쑥 분말 첨가 농도가 높아질수록 수분 함량은 낮아졌으며 탄수화물과 회분 함량은 다소 증가하는 경향을 보였다. 총 유리아미노산 함량은 개똥쑥 분말을 첨가할수록 유의적으로 증가하는 경향을 보여 개똥쑥 분말 첨가가 절편의 유리아미노산 함량에 영향을 주는 것으로 판단된다. 색도를 분석한 결과 명도를 나타내는 L값의 경우 개똥쑥 분말 첨가량이 증가하면서 감소하는 경향을 보였고, 녹색도를 나타내는 -a값, 황색도를 나타내는 b값은 개똥쑥 분말 첨가량이 증가할수록 증가하는 경향을 보였다. 유리당을 분석한 결과 sucrose만 검출되었으며 개똥쑥 분말 첨가량이 많아지면서 sucrose 함량이 증가하는 결과를 보였으며, 식이섬유 함량도 첨가량에 따라 증가하는 것으로 나타났다. 관능에 따른 시료별 선호도 결과 색(color)은 90 g 첨가 시료가 향미(flavor), 맛(taste), 식감(chewiness)은 30 g 첨가 시료가 가장 높은 값을 나타냈다. 전반적 기호도와 다양한 관능검사결과를 종합하여 볼 때 개똥쑥 분말 30 g을 첨가하여 제조한 개똥쑥 떡의 선호도가 가장 높다는 것을 알 수 있다.

• 한국약용작물학회지
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• 제18권1호
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• pp.46-50
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• 2010

In order to development medicinal Artemisia herbs of high quality, Korean A. annua L. were investigated with its ecological and morphological characteristics, and basic statistical data of agronomic characteristics. This species, which is annual herb, is mainly distributed to marginal land, riverside, roadside, grassland. Ecological niche is low species in competition of the others. Its pollination is basically anemogamous, but is frequency pollinated by insects. This species is characterized as tap root, 1~3 pinnate compound leaf of ovate or narrowly ovate, stem is green, erect and solitary. Inflorescence is paniculate, receptacle is not hair. Capitulum, consist of ray floret and disk floret, is subglobose shape. Additionally, this species could be easily discriminated from related Artemisia herbs by the capitulum size. Ray floret is female, disk floret is bisexual. Flowering season is from August to October. Seed is achene unattached hair. Stem length ranged from 179 cm to 225 cm, and stem diameter and number of branch were $17.14\;{\pm}\;1.68\;mm$, $2.43\;{\pm}\;0.51\;mm$, respectively. Length and width of leaf were $14.5\;{\pm}\;0.5\;cm$, $15.0\;{\pm}\;1.0\;cm$, and leaf number of main stem were $48.06\;{\pm}\;10.57\;cm$, respectively. Fresh weight of aerial parts and root were $364.7\;{\pm}\;14.1\;g$, $32.6\;{\pm}\;5.1\;g$, and its dry weight were $136.6\;{\pm}\;10.0\;g$, $14.9\;{\pm}\;2.34\;g$, respectively.

• Applied Biological Chemistry
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• 제35권2호
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• pp.106-109
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• 1992

국화과의 일증인 개똥쑥(Artemisia annua L., 청호(靑蒿))에는 항말라리아 물질인 artemisinin이 함유되어 있다. 11,12-Dihydroarteanuic acid는 cell-free 방법으로 수행한 실험에서, Agrobacterium tumefaciens로 형질 전환된 crown gall의 homogenate와의 incubation에서 artemisinin으로 전환되었다. 그러나 arteannuic acid는 NADPH와 함께 incubation하였을 때 dihydro acid로 전환되지 않았다. 따라서 이 결과는 식물체의 조건에 따라서, dihydro acid가 자발적으로 뿐만 아니라, 생체내에서 효소 반응에 의해서도 artemisinin으로 전환될 수 있음을 나타낸다.

• Journal of Plant Biotechnology
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• 제34권3호
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• pp.197-200
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• 2007

Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

• 한국식품영양과학회지
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• 제40권2호
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• pp.163-170
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• 2011

개똥쑥(Artemisia annua L.)의 기능성을 밝히고 식품으로써 이용성을 증대시키기 위하여 개똥쑥 잎과 줄기의 영양 성분 및 생리활성을 측정하였다. 수분, 조지방 및 조단백질 함량은 잎에서 높았으나 회분, 조섬유 및 무기물 함량은 줄기에서 유의적으로 높았다. 총 페놀과 플라보노이드 함량은 잎이 줄기에 비해 약 2배 정도 높았다. 추출물의 농도를 달리하여 항산화능을 측정한 결과 농도에 의존적으로 활성이 증가하였으며, 잎은 줄기에 비해 활성이 높았다. 특히, DPPH 라디칼 소거능, 환원력 및 tyrosinase 저해활성은 모든 농도에서 잎이 줄기에 비해 유의적으로 높았으나, ${\alpha}$-glucosidase 저해활성은 줄기 추출물이 더 높았다. 인체 유방암 세포인 MCF-7 및 MDA-MB-231에 대한 증식억제 활성은 줄기보다 잎 추출물에서, 물보다 에탄올 추출물에서 유의적으로 활성이 높았다. 특히, 잎 에탄올 추출물의 MCF-7 및 MDAMB-231 세포에 대한 증식억제 활성은 $250\;{\mu}g$/mL에서 각각 76.26% 및 52.59%로 추출물 중 가장 높은 활성을 보였다. 이상의 결과, 개똥쑥 추출물의 생리활성은 시료 중의 섬유소, 페놀 및 플라보노이드 함량에 의존적인 것으로 생각된다.

• 한국환경농학회지
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• 제33권3호
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• pp.194-197
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• 2014

BACKGROUND: Herbal extracts have been screened for their inhibitory effect of seed germination and root development on weeds, but there is a scarcity of reports for crop growth regulation. The objective of this research was to develop a growth inhibitor on Brassica campestris, and its effective extraction method from herbal medicine extract. METHODS AND RESULTS: Eighty four herbal medicine extracts were tested for their plant growth inhibition activity on B. campestris. The alcohol extracts of Artemisia annua, Cinnamomum cassia, and Mentha arvensis inhibited over 30% of germination and the extract of A. annua, and C. cassia inhibited over 70% of radicle growth at 0.1 % w/w treatment. The partially purified extracts of A. annua, and C. cassia with dichloromethane and hexane showed stronger radicle growth inhibition than the crude extracts on B. campestris. The diethyl ether extract of A. annua showed a similar 50% radicle growth inhibition ($RI_{50}$ = 45 mg/L) to its partially purified extract with dichloromethane or hexane, but the diethyl ether extract of C. cassia showed a worse $RI_{50}$ than the purified extract. CONCLUSION: The alcohol extracts of A. annua, and C. cassia showed potent radicle growth inhibition properties on B. campestris. Diethyl ether proved to be a good solvent for simple extraction from A. annua.

• 한국동물위생학회지
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• 제43권1호
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• pp.7-16
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• 2020

This study investigated the effects of the dietary supplementation with gaeddongssuk (Artemisia annua L.) powder on blood biochemical compositions, meat lipids and fatty acid profiles of the broiler chicks. One hundred male broiler chicks were divided randomly into five groups: group fed with basal diet (Control); group supplemented with 2.5% antibiotics in the drink water (Antibiotics), and groups supplemented gaeddongssuk powder with 5% (BG-I), 6% (BG-II), and 7% (BG-III) in the basal diets. Levels of total lipid and LDL-C in serum of broiler chicks were significantly lower in the groups supplemented with gaeddongssuk compared to the Antibiotics group. Contents of triglyceride and total cholesterol were significantly lower in the BG-III. HDL-C level was significantly higher in BG-I and BG-II compared to the Antibiotics group. Antioxidant activity of serum in the BG-II was significantly higher than Control and Antibiotics groups. Lipid peroxide contents in the BG-I and BG-II were significantly lower than to the Antibiotics group. Total lipids level of breast and legs meat was significantly lower in the groups supplemented gaeddongssuk compared to the Antibiotics group. Total cholesterol level of breast meat was significantly lower in the groups supplemented with gaeddongssuk compared to the Antibiotics group. UFA/SFA ratio of breast and legs meat from the BG-II was tend to higher compared to Control and Antibiotics groups. Taken together, these results suggest that dietary supplementation of gaeddongssuk with 6% could be applicable as the possibility to improve blood biochemical compositions and meat lipids properties in broiler chicks.

• 한국토양비료학회지
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• 제44권1호
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• pp.29-37
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• 2011

The experiment was carried out to find the effects of transplanting time and vinyl-film mulching treatment on the growth of artemisia by randomized block design with three replications. The experiment site ($100{\times}130$ m) was temporally established in the south-eastern part of Saemangeum reclaimed tidal land (near Gwanghwal myun, Gimjae-gun, Jellabukdo). Artemisia plants had been partly suffered from salt injury, because soil salinities in some area during growing period had been measured higher than 10 dS $m^{-1}$. Growth of plant height and survival ratio of transplanted plants had been significantly correlated with soil salinity and then the regression equations between plant height (y) and soil EC (x) and between survival ratio (y) and soil EC (x) were expressed as y=-16.59ln(x)+43.852 and $y=0.6453x^2-17.566x+103.99$, respectively. It was concluded that early transplanting and vinyl mulching was more beneficial for biomass production of artemisia, because biomass was 6.41 times more in the early transplanting than in the late transplanting, and 2.63 times more in the vinyl-film mulching than in the no mulching treatment.

• Bulletin of the Korean Chemical Society
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• 제29권7호
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• pp.1386-1390
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• 2008

Artemisinin, the effective ingredient of Chinese herb Artemisia annua L (Qinghao in Chinese), has been proved to be effective to antimalarial. Herein, a reliable, sensitive and convenient electrochemical method was developed for the determination of artemisinin utilizing the excellent properties of multi-wall carbon nanotube (MWNT). The electrochemical behavior of artemisinin was investigated. It is found that the reduction peak current of artemisinin remarkably increases and the peak potential shifts positively by 240 mV at the MWNT film-modified electrode. These phenomena indicate that the MWNT film exhibits efficient catalytic activity to the electrochemical reduction of artemisinin. The effects of pH value, amount of MWNT, scan rate and accumulation time were examined. The limit of detection (S/N = 3) is as low as 10 $\mu$ g $L^{-1}$. Finally, this newly developed method was used to determine the content of artemisinin in Artemisia annua L.