• Asian-Australasian Journal of Animal Sciences
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• 제19권3호
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• pp.412-417
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• 2006

This study evaluated the effectiveness of different arsenical sources on inducing fatty liver, on changes in lipid metabolism and on liver function in mule ducks. Sixty twelve-week-old mule ducks were selected and randomly divided into five treatments, including the control group and four different arsenical sources; Roxarsone (300 mg/kg), arsanilic acid, $As_2O_5$ or $As_2O_3$, containing 85.2 mg/kg arsenic were included in the basal diet. The ducks were fed the medicated basal diet for 3 weeks followed by a one-week drug withdrawal. The results showed Roxarsone treatment decreased body weight, feed intake, liver weight and abdominal fat weight (p<0.05), while it increased the relative liver weight (p<0.05) during medication period ($3^{rd}$ week). The $As_2O_5$ treatment decreased abdominal fat weight and relative abdominal fat weight when compared to the control (p<0.05). Only Roxarsone among the treatment groups increased feed intake, liver weight and relative liver weight, while the $As_2O_3$ group showed the lightest liver weight and relative liver weight among treatment groups during the withdrawal period ($4^{th}$ week). The Roxarsone group decreased (p<0.05) NADP-malic dehydrogenase (MDH) and acetyl-CoA carboxylase (ACC) activities and increased (p<0.05) cholesterol concentration during the medication period, and elevated the MDH and ACC activities during the withdrawal period. All four arsenical treatment groups showed lymphocytic infiltration in liver tissue, while the Roxarsone and $As_2O_3$ treatments showed an increase in aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) activities (p<0.05). During the withdrawal period, arsenical treatments resulted in liver vacuoles. However, the arsenicals differed in effectiveness and mechanisms of inducing fat vacuoles.

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.31-38
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• 2002

Bacteria have evolved various types of resistance mechanism to toxic heavy metals, such as arsenic and antimony. An arsenical and antimonial resistant bacterium was isolated from a shallow creek draining a coal-mining area near Taebaek City, in Kangwon-Do, Korea. The isolated bacterium was identified and named as Pseudomonas sp. KM20 after biochemical and physiological studies were conducted. A plasmid was identified and its function was studied. Original cells harboring the plasmid were able to grow in the presence of 15 mM sodium arsenite, while the plasmid-cured (plasmidless) strain was sensitive to as little as 0.5 mM sodium arsenate. These results indicated that the plasmid of Pseudomonas sp. KM20 does indeed encode the arsenic resistance determinant. In growth experiments, prior exposure to 0.1 mM arsenate allowed immediate growth when they were challenged with 5 mM arsenate, 5 mM arsenite, or 0.1 mM antimonite. These results suggested that the arsenate, arsenite, and antimonite resistance determinants of Pseudomonas sp. KM20 plasmid were indeed inducible. When induced, plasmid-bearing resistance cells showed a decreased accumulation $of\;73^As$ and showed an enhanced efflux $of\;^73As$. These results suggested that plasmid encoded a transport system that extruded the toxic metalloids, resulting in the lowering of the intracellular concentration of toxic oxyanion. In a Southern blot study, hybridization with an E. coli R773 arsA-specific probe strongly suggested the absence of an arsA cistron in the plasmid-associated arsenical and antimonial resistance determinant of Pseudomonas sp. KM20.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.593-598
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• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• Asian Pacific Journal of Cancer Prevention
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• 제17권9호
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• pp.4185-4197
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• 2016

Chronic arsenicosis is a major environmental health hazard throughout the world, including India. Animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. Arsenic causes cancer with production of free radicals and reactive oxygen species (ROS) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidants. Dietary antioxidant supplements are useful to counteract the carcinogenesis effects of arsenic. Oyster mushroom lectins can be regarded as ingredients of popular foods with biopharmaceutical properties. A variety of compounds have been isolated from mushrooms, which include polysaccharides and polysaccharopeptides with immune-enhancing effects. Lectins are beneficial in reducing arsenic toxicity due to anticarcinogenetic roles and may have therapeutic application in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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• pp.159-164
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• 2001

A novel fluidized-bed reactor was designed and installed for bioleaching in a semi-continuous way, by which a process for bioleaching-cyanidation of Guangxi Jinya refractory gold arsenical concentrate was studied. An arsenic extraction rate reaches 82.5% after 4-day batch biooxidation of the concentrate under the optimized condition of pH 2.0, ftrric ion concentration 6.5g/L and pulp concentration 10%. And leaching rate of gold in the following gold cyanidation is over 90%. The parameters of three series fluid-bed reactors exhibit stability during the semi-continuous bioleaching of the concentrate. Arsenic in the concentrate can be got rid of 91% after 6-day leaching. Even after 4 days, 82% of arsenic extraction rate was still obtained. The recovery rates of gold are 92% and 87.5% respectively in cyaniding the above bioleached residues. The results will provide a base for further commercial production of gold development.

• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1375-1382
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• 2016

The extremely halophilic archaeon Halobacterium noricense is a member of the genus Halobacterium. Strain CBA1132 (= KCCM 43183, JCM 31150) was isolated from solar salt. The genome of strain CBA1132 assembled with 4 contigs, including three rRNA genes, 44 tRNA genes, and 3,208 open reading frames. Strain CBA1132 had nine putative CRISPRs and the genome contained genes encoding metal resistance determinants: copper-translocating P-type ATPase (CtpA), arsenical pump-driving ATPase (ArsA), arsenate reductase (ArsC), and arsenical resistance operon repressor (ArsR). Strain CBA1132 was related to Halobacterium noricense, with 99.2% 16S rRNA gene sequence similarity. Based on the comparative genomic analysis, strain CBA1132 has distinctly evolved; moreover, essential genes related to nitrogen metabolism were only detected in the genome of strain CBA1132 among the reported genomes in the genus Halobacterium. This genome sequence of Halobacterium noricense CBA1132 may be of use in future molecular biological studies.

• 한국기술사회:학술대회논문집
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• 한국기술사회 1983년도 한일기술사 합동 심포지움
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• pp.74-77
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• 1983

To attain the rich and comfortable human living, much development and economic growth are needed. But the radical development destroys natural environments or resources, and also the life environments are destroyed. How to maintain the balance between development and environments forms an important problem. Since 1955, a great deal of damage has been done to human being by the pollution of air, water or noise in Japan, for example, Minamata-byo (1956), arsenical poisoning by mkil (1955), Cd-I taiitai-byo (1955), asthma by air pollution, etc. Now, there are over 100,000 sick persons suffering from pollution in Japan. To prevent the pollution, the first act was made 1967, the kind of which were defined as follow:air, water, soil, noise, vibration ground subsidence, and bad smell. Laws on these were established 1970∼1981, and the environments here ave gradually being improved. The mentioned above gituation necsesitates the measuring or chemical analysis of pollution data, but the many cost is necessary to obtain such data. The special environment measuring certifier was needed and the Measurement Law was amended 1974. Some comment will be given of the present condition of environmental measuring certifier.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 The 6th International Symposium of East Asian Resources Recycling Technology
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• pp.183-187
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• 2001

Present states on reutilization of metal-bearing solid wastes in China including metal-containing gangue, red mud, nonferrous metallurgical slag or residue, arsenical slag, steel - iron slag, waste batteries, were described in detail. The wastes pile up at a large quantity, resulting in seriously potential harm to environment. Most of these wastes, however, contain valuable metals, which are regarded as important secondary resources for extracting metals. Waste slag and batteries with a high grade of metals are treated by a hydro-based and / or pyre-based method for extracting valuable metals. While gangue and waste slag with a low grade are as a raw material in architecture field. In the future, a novel technology, such as high-grads magnetization separation technique and biological technique, will be designed to treat these wastes for protecting environment and recycling valuable components. These wastes, furthermore, are synthetically reutilized to produce various architectural materials, including glass and ceramics.

• 박물관보존과학
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• 제13권
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• pp.13-22
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• 2012

국립중앙박물관 소장 적의본과 폐슬본은 20세기 초에 제작된 것으로 모두 지본채색되어 있다. 두 본에 사용된 채색 안료 및 염료에 대한 비파괴분석을 통해 채색 재료를 확인하고자 하였다. 채색 안료의 성분분석에는 XRF를 이용하였다. 적의본의 안료 분석결과 백색은 연분, 흑색은 먹, 황색은 석황, 적색은 진사와 연단, 녹색은 구리와 비소화합물 또는 석청과 석황을 혼합하여 사용한 것으로 추정되며 기타색상으로 황동을 사용하였다. 폐슬본에 사용된 백색, 흑색, 황색, 녹색은 적의본에 사용된 안료와 동일하나 적색은 채색 안료에 차이를 보였다. 한편 적의본과 폐슬본에 사용된 청색은 안료의 종류를 확인이 어려우나 연분과 혼합하여 사용한 것으로 추정된다. 또한 적의본의 청색 한지에 대한 UV-Vis 분광분석 결과 인디고(쪽)으로 염색한 것으로 추정되었다.