• 대한예방한의학회지
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• 제15권1호
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• pp.37-47
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• 2011

Objectives : Previous studies have shown that Fructus mume (FM) has anti-platelet effects. The present study was performed to determine the acute oral toxicity and quality control of a crude extract of FM in ICR mice. Methods : We investigated the in vivo single dose acute toxicity of FM 95% ethanol extract. This test was orally administered once by gavage to 20 male and 20 female mice at dose levels of 0 (control group), 1250, 2500 and 5000mg/kg body weight, respectively. Mortalities, clinical findings, autopsy findings and body weight changes were monitored daily for the 14 days following the administration. HPLC analysis was performed for the simultaneous determination of ursolic acid and p-hydroxycinnamic acid in FM. Reverse-phase chromatography using a C18 column and photodiode array detection at 211 nm was used for quantification of the two maker components. The mobile phase for gradient elution consists of water and acetonitrile. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. The mice did not die after single oral administration of maximum dose of FM. Autopsy of animal revealed no abnormal gross finding. Therefore, $LD_{50}$ value of FM for ICR mice was more than 5000mg/kg on oral route. The HPLC analysis showed that ursolic acid and p-hydroxycinnamic acid amounts to 9.75- and 0.12% in the extract with the retention times of 47.99- and 15.38 minutes, respectively. Conclusions : These results suggest that no toxic dose level of FM in mice is considered to be more than 5000mg/kg. Consequently, it was concluded that FM have no effect on acute toxicity and side effect in ICR mice. For the quality control of FM extract, simultaneous determination of ursolic acid and p-hydroxycinnamic acid was established.

• 지구물리와물리탐사
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• 제8권1호
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• pp.26-33
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• 2005

최근 들어 토목관련 천부층 조사에 다중 모드 표면파 위상 속도의 역산이 많은 관심을 받고 있다. 감도 분석, 그리고 합성탄성파자료와 현장자료의 역산 결과는 이 방법이 기본 모드만을 이용하는 것에 비해 매우 효과적임을 보여주고 있다. 이중 모드 레일리 파의 위상속도들에서 층의 두께와 전단파 속도에서의 조그만 변화는 고차 모드의 감도들을 (a) 다른 주파수 대역들에 모이게 하고 (b) 심도가 깊어질수록 기본 모드보다도 더 크게 한다. 이 관찰을 통해 다중 모드 위상 속도 역산을 이용하면 기본 모드 자료들만의 역산에 비해 변수값들을 더 잘 구분해 낼 수 있고 깊은 구조, 특히 속도 역전이 일어난 구조에 대해 보다 나은 영상을 얻을 수 있음을 알 수 있다. 20 m 깊이에 저속도층이 존재하는 모델에서 이론적인 위상 속도들의 역산은 1차 모드만 첨가될 때 단지 연암층만을 영상화할 수 있다. 이 사실은 측정 가능한 가장 낮은 주파수가 단지 6 Hz 일 때 특히 중요하다. 현장시험들이 시추공과 PS 검층을 이용하여 조사된 지역들에서 행해졌다. 첫 번째 지역에서는 일본에서 심부 지질조사에 주로 이용되는 microtremor 배열 탐사가 35 m 깊이까지 토양층을 탐사하기 위해 사용되었다. 두 번째 지역에서는 12 m 깊이까지 조사하기 위해 sledgehammer 음원과 선형 다중 채널 수진기 전개를 이용하여 자료가 얻어졌다. 분산곡선 분석을 위해서 주파수-파수 파워 스펙트럼법이 사용되었고 각각의 시험에서 2차 모드의 속도까지 구해졌다. 다중 모드 역산 결과는 PS 검층기록과 잘 일치한다. 하지만 단지 기본 모드만을 이용하여 얻어진 결과는 매립지 아래의 천부 연암층까지의 깊이를 매우 작게 평가하였다.

• 천문학회지
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• 제52권1호
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• pp.23-30
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• 2019

The KVN(Korean VLBI Network)-style simultaneous multi-frequency receiving mode is demonstrated to be promising for mm-VLBI observations. Recently, other Very long baseline interferometry (VLBI) facilities all over the globe start to implement compatible optics systems. Simultaneous dual/multi-frequency VLBI observations at mm wavelengths with international baselines are thus possible. In this paper, we present the results from the first successful simultaneous 22/43 GHz dual-frequency observation with KaVA(KVN and VERA array), including images and astrometric results. Our analysis shows that the newly implemented simultaneous receiving system has brought a significant extension of the coherence time of the 43 GHz visibility phases along the international baselines. The astrometric results obtained with KaVA are consistent with those obtained with the independent analysis of the KVN data. Our results thus confirm the good performance of the simultaneous receiving systems for the nonKVN stations. Future simultaneous observations with more global stations bring even higher sensitivity and micro-arcsecond level astrometric measurements of the targets.

• 한국식품위생안전성학회지
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• 제34권4호
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• pp.334-339
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• 2019

본 연구는 조제유류 중 "식품의 기준 및 성분규격"에 기준 규격이 설정되어 있지 않으나 제품에 함유되어 있는 베타카로틴에 대해 분석법을 마련하고자 수행하였다. 조제유류에 함유된 베타카로틴 함량 분석을 위해 HPLC를 이용한 분석법을 확립하고 시중에 유통 중인 제품을 대상으로 적용성을 검토하였다. 베타카로틴 표준품을 이용하여 HPLC를 이용한 기기분석조건을 확립하고 시료 중의 베타카로틴을 추출하여 분석하였다. 분석법 검증은 특이성, 직선성, 검출한계 및 정량한계, 정확성, 정밀성에 대해 수행되었다. 0.125~2 mg/L의 농도범위에서 $R^2=0.999$ 이상의 우수한 직선성을 확인할 수 있었으며, LOD와 LOQ는 각각 0.1, 0.2 mg/L였다. 표준물질 첨가법을 이용하여 정확성을 검토하였으며, 81~120%의 회수율을 확인할 수 있었다. 정밀성을 검토한 결과 시료 채취량에 따른 반복성은 RSD값이 2.1~4.9%, 실험실간 교차검증을 통한 실험일자에 따른 재현성은 4.0 RSD(%)로 확인되었다. 본 연구에서 확립된 분석법을 적용하여 조제유류 13건, 성장기용조제식 7건 등 국내 유통 중인 제품 20건에 대해 적용성 검토를 실시한 결과 전체 시료에서 분석이 용이하였으며, 모두 표시기준에 적합함을 확인하였다.

• 동의생리병리학회지
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• 제34권6호
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• pp.348-354
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• 2020

Oryeong-san (ORS) is a traditional Korean herbal medicine widely used for renal associated diseases, composed of five medicine herbs; Atractylodes japonica Koidzumi, Cinnamomum cassia Presl, Polyporus umbellatus Fries, Poria cocos Wolf and Alisma orientale Juzepzuk. We studied to improve the convenience of intake and portability by developing modernized dosage forms, and examined the effect on anti-inflammation of ORS. In order to develop the tablet formulation of ORS (ORS-F), the tablets were evaluated on the basis of physical characteristics include diameter, thickness, weight variation, hardness, friability and disintegration. To analyze the marker components of ORS-F, eight index markers from five herbal medicines were chosen. And the method using high performance liquid chromatography (HPLC) with diode-array detector method was established for the simultaneous analysis. The biological activities were examined the effect of ORS-F on pro-inflammation mediated by LPS-stimulation. The production of nitric oxide (NO) and cytokines were determined by reacting cultured medium with griess reagent and enzyme-linked immunosorbent assay (ELISA). The expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) were investigated by Western blot and RT-PCR. The anti-oxidant activities of OJS-F increased markedly, in a dose-dependent manner. and, The total phenolic compound and flavonoids contents of OJS-F were 10.20±0.09 ㎍/㎎ and 12.86±0.86 ㎍/㎎. OJS-F which is LPS has diminished in the LPS-induced release of inflammatory mediators (NO, iNOS, COX2 and PGE2) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) from the RAW264.7 macrophages. Therefore, the developed formulation for tablet of ORS-F provide efficiency and usability, and indicated effect of anti-inflammation.

• 한국지반공학회논문집
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• 제23권2호
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• pp.47-60
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• 2007

해머나 중추 등의 순간가진원이나 진동기 등의 연속가진원을 사용하는 주동표면파기법에서는 저주파대역에서 충분한 에너지를 갖는 표면파를 얻기 어렵기 때문에, 종종 발생된 표면파의 가탐심도가 충분하지 못한 한계에 부딪힌다. 일반직인 가진원 장비로 발생되는 이러한 주동표면파의 한계는 심층지반구조를 파악할 수 없게 함으로써 지반의 정확한 지진응답해석을 어렵게 한다. 반면에 상시미동이나 주변잡음 등과 같은 수동가진원에 의해 발생된 수동표면파를 이용하면 저주파대역에서 충분한 에너지를 갖는 표면파를 얻을 수 있기 때문에 이러한 주동표면파의 단점을 극복하고 심층지반까지의 전단파속도 주상도를 얻을 수 있다. 수동표면파의 측정자료로부터 계산된 수동분산값의 신뢰도를 향상시키는 것은 보다 정확한 전단파속도 주상도를 얻기 위해 필수적이며, 수동분산값의 신뢰도를 향상시키기 위해선 수동분산값에 포함되는 계통오차에 대한 올바른 이해를 바탕으로 한 수동표면파의 측정과 분석이 필요하다. 본 논문에서는 수동표면파 시험에 있어 계통오차를 발생시키는 부족한 파수해상도와 측면로브를 통한 에너지누수에 관한 연구를 수행하고 신뢰성 있는 분산곡선 결정을 위한 수동표면파의 측정 및 분석기법을 제안한다. 제안된 기법을 적용한 일련의 수동표면파시험이 미국 캘리포니아주 산호세의 한 현장에서 수행되었다.

• 천문학회지
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• 제56권2호
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• pp.231-252
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• 2023

We present the analysis results of the simultaneous multifrequency observations of the blazar 4C +28.07. The observations were conducted by the Interferometric Monitoring of Gamma-ray Bright Active Galactic Nuclei (iMOGABA) program, which is a key science program of the Korean Very Long Baseline Interferometry (VLBI) Network (KVN). Observations of the iMOGABA program for 4C +28.07 were conducted from 16 January 2013 (MJD 56308) to 13 March 2020 (MJD 58921). We also used γ-ray data from the Fermi Large Array Telescope (Fermi-LAT) Light Curve Repository, covering the energy range from 100 MeV to 100 GeV. We divided the iMOGABA data and the Fermi-LAT data into five periods from 0 to 4, according to the prosody of the 22 GHz data and the presence or absence of the data. In order to investigate the characteristics of each period, the light curves were plotted and compared. However, a peak that formed a hill was observed earlier than the period of a strong γ-ray flare at 43-86 GHz in period 3 (MJD 57400-58100). Therefore, we assumed that the minimum total CLEANed flux density for each frequency was quiescent flux (S_q) in which the core of 4C +28.07 emitted the minimum, with the variable flux (S_var) obtained by subtracting S_q from the values of the total CLEANed flux density. We then compared the variability of the spectral indices (α) between adjacent frequencies through a spectral analysis. Most notably, α_22-43 showed optically thick spectra in the absence of a strong γ-ray flare, and when the flare appeared, α_22-43 became optically thinner. In order to find out the characteristics of the magnetic field in the variable region, the magnetic field strength in the synchrotron self-absorption (B_SSA) and the equipartition magnetic field strength (B_eq) were obtained. We found that B_SSA is largely consistent with B_eq within the uncertainty, implying that the SSA region in the source is not significantly deviated from the equipartition condition in the γ-ray quiescent periods.

• Korean Journal of Radiology
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• 제23권5호
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• pp.539-547
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• 2022

Objective: To investigate the association between functional tumor burden of peritoneal carcinomatosis (PC) derived from diffusion-weighted imaging (DWI) and overall survival in patients with advanced ovarian carcinoma (OC). Materials and Methods: This prospective study was approved by the local research ethics committee, and informed consent was obtained. Fifty patients (mean age ± standard deviation, 57 ± 12 years) with stage III-IV OC scheduled for primary or interval debulking surgery (IDS) were recruited between June 2016 and December 2021. DWI (b values: 0, 400, and 800 s/mm²) was acquired with a 16-channel phased-array torso coil. The functional PC burden on DWI was derived based on K-means clustering to discard fat, air, and normal tissue. A score similar to the surgical peritoneal cancer index was assigned to each abdominopelvic region, with additional scores assigned to the involvement of critical sites, denoted as the functional peritoneal cancer index (fPCI). The apparent diffusion coefficient (ADC) of the largest lesion was calculated. Patients were dichotomized by immediate surgical outcome into high- and low-risk groups (with and without residual disease, respectively) with subsequent survival analysis using the Kaplan-Meier curve and log-rank test. Multivariable Cox proportional hazards regression was used to evaluate the association between DWI-derived results and overall survival. Results: Fifteen (30.0%) patients underwent primary debulking surgery, and 35 (70.0%) patients received neoadjuvant chemotherapy followed by IDS. Complete tumor debulking was achieved in 32 patients. Patients with residual disease after debulking surgery had reduced overall survival (p = 0.043). The fPCI/ADC was negatively associated with overall survival when accounted for clinicopathological information with a hazard ratio of 1.254 for high fPCI/ADC (95% confidence interval, 1.007-1.560; p = 0.043). Conclusion: A high DWI-derived functional tumor burden was associated with decreased overall survival in patients with advanced OC.

• BMB Reports
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• 제57권3호
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• pp.161-166
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• 2024

Aberrant DNA methylation plays a critical role in the development and progression of colorectal cancer (CRC), which has high incidence and mortality rates in Korea. Various CRC-associated methylation markers for cancer diagnosis and prognosis have been developed; however, they have not been validated for Korean patients owing to the lack of comprehensive clinical and methylome data. Here, we obtained reliable methylation profiles for 228 tumor, 103 adjacent normal, and two unmatched normal colon tissues from Korean patients with CRC using an Illumina Infinium EPIC array; the data were corrected for biological and experiment biases. A comparative methylome analysis confirmed the previous findings that hypermethylated positions in the tumor were highly enriched in CpG island and promoter, 5' untranslated, and first exon regions. However, hypomethylated positions were enriched in the open-sea regions considerably distant from CpG islands. After applying a CpG island methylator phenotype (CIMP) to the methylome data of tumor samples to stratify the CRC patients, we consolidated the previously established clinicopathological findings that the tumors with high CIMP signatures were significantly enriched in the right colon. The results showed a higher prevalence of microsatellite instability status and MLH1 methylation in tumors with high CMP signatures than in those with low or non-CIMP signatures. Therefore, our methylome analysis and dataset provide insights into applying CRC-associated methylation markers for Korean patients regarding cancer diagnosis and prognosis.

• Clinical and Experimental Pediatrics
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• 제49권4호
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• pp.439-445
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• 2006

목 적 : ${\alpha}$-Galactosylceramide (GalCer)로 자극한 자연살상 T 세포(NKT)는 CD1d 및 T 세포 수용체(T cell receptor) 의존적으로 일부 백혈병에서 항암효과를 발현하나, CD1d음성인 신경모세포종에서는 세포독성을 유도할 수 없다. 이들 NKT세포의 활성화 시 분비되는 많은 양의 사이토카인의 직접적인 항암효과에 대해서는 소수의 보고가 있다. 본 연구에서는 hCD1d/${\alpha}$-GalCer tetramer로 NKT세포를 자극하여 얻은 상청액(supernatant)을 이용하여 NKT세포에 의한 신경모세포종의 치료적 접근의 가능성을 알아보았다. 방 법 : 신경모세포종 세포 주를 IMDM 배지에 배양하였고, NKT세포에서 분비되는 사이토카인 양은 cytometric bead array (CBA)분석으로 측정하였다. 세포 생존율은 calcein-AM 형광물질을 이용하여 digital image microscopy scanning (DIMSCAN)으로 측정하였고 특이 세포고사(specific apoptosis)는 annexin V and 7-AAD 염색 후 유식세포분석기를 통하여 산출하였다. 결 과 : 활성화된 NKT세포는 많은 양의 IL-2, IL-4, INF-${\gamma}$와 TNF-${\alpha}$을 분비하였다. NKT 자극 후 얻어진 상청액은 8개의 신경모세포종 세포 주 중 4개에서 의미있는 세포독성을 나타냈으며, 그 기전은 annexin-V 염색이나 pancaspase 억제제의 전 처치 실험으로 세포고사을 통하여 유도됨을 알 수 있었다. 그리고 이들 세포고사 유도는 anti-TNF-${\alpha}$, anti-IFN-${\gamma}$ 중화항체의 단독투여 시 현저히 감소하였고 동시투여 시에는 완전하게 억제되었다. 결 론 : NKT 세포의 활성화에 의해 분비된 IFN-${\gamma}$와 TNF${\alpha}$가 일부 신경모세포종 세포 주에서 협동적 세포 독성을 유도하였다.