• The Korea Journal of Herbology
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• v.29 no.6
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• pp.35-43
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• 2014

Objectives : Official Arisaematis Rhizoma is described only three species, Arisaema amurnse, Arisaema erubescens, and Arisaema heterophyllum, in national Pharmacopoeia. However, other Arisaema species, Arisaema ringens, Arisaema takesimense and Arisaema serratum, also have been distributed as an inauthentic Arisaematis Rhizoma in the herbal market. To develop a reliable molecular authentication method for Arisaematis Rhizoma in species level, we analyzed DNA barcode regions using six Arisaema species. Methods : Thirty-eight samples of six Arisaema plants species (A. amurense, A. amurense f. serratum, A. heterophyllum, A. takesimense, and A. serratum) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL gene) were analyzed after PCR amplification. The species-specific sequences and phylogenetic relations were estimated using entire sequences of three DNA barcodes based on the analysis of ClastalW and UPGMA, respectively. Results : The comparative analysis of DNA barcode sequences were revealed inter-species specific nucleotides to distinguish the medicinal plant of Arisaema Rhizoma in species levels excluding between A. amurense and its subspecies (A. amurense f. serratum) and A. takesimense and A. serratum, respectively. However, we obtained sequence differences enough to discriminate authentic and inauthentic Arisaematis Rhizoma. Therefore, we suggest that these SNP type molecular genetic markers were an reliable method avaliable to identify official herbal medicines. Conclusions : These marker nucleotides could be useful to identify the official herbal medicines by providing definitive information that can identify original medicinal plant and distinguish from inauthentic adulterants and substitutes.

• Korean Journal of Plant Resources
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• v.31 no.1
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• pp.37-51
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• 2018

Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, Sinarisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2002.04b
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• pp.173-173
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• 2002

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.1
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• pp.16-22
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• 2007

This study was carried out to investigate the effects of shading conditions for appropriate light level for several floricultural plants native to Korea. The results under the different shading levels of 0, 30, 50, 60% and full sun light are as follows; Growth of Miscanthus sinensis and Pennisetum alopecuroides, which are heliophyte plants, were better under 30% shading condition than full sunlight. Higher shading levels was poor than control. Growth of Sedum spectable was decreased when light intensity was decreased. As shading level increased, growth reduction rate in Sedum spectable were higher than that of other species. Growth and ornamental value of Arisaema amurense, which is sciophyte plants, were increased under 50% shading.

• Korean Journal of Environment and Ecology
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• v.15 no.4
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• pp.293-318
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• 2002

As one of the botanical hot spots of the Korean peninsula, the vascular plant species in the areas of Taebaeksan, Hambaeksan, Geumdaebong(Peak) and Maebongsan were summarized as 694 taxa, 89 families, 302 genus, 579 species. 102 varieties and 13 forms. Based on the list of rare and endangered plants from the Forest Research Institute and the Ministry of Environment, 22 species were recorded in the studied areas: Thuja koraiensis(Cupressaceae), Arisaema heterophyllum(Araceae), Disporum ovale(Liliaceae), Tricyrtis dialata(Liliaceae), Lilium distichum(Liliaceae), Anemone koraiensis(Ranunculaceae), Rodgersia podophylla(Saxifragaceae), Rosa marretii(Rosaceae), Viola diamantica(Violaceae), Viola albida(Violaceae), Echinopanax horridum(Araliaceae), Acanthopanax chiisannensis(Araliaceae), Lysimachia coreana(Prinmulaceae), Syringa velutina var. kamibayashii, Halenia corniculata(Gentianaceae), Partrinia saniculaefolia(Valerianaceae), Adenophora grandiflora(Campanulaceae), Cacalia pseudo-taimingasa(Compositae) Iris odaesanensis(Iridaceae), Leontice microrhyncha(Berberidaceae), Rpdgersia tabularis(Saxifragaceae), and Acanthopanax sentico년(Araliaceae), Among them the four species were recorded from the list of the Ministry of Environment. As most of the species were recorded from the mountain-trails, the long-term habitat monitoring for the species is required .

• Korean Journal of Plant Resources
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• v.13 no.1
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• pp.61-65
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• 2000

It was investigated 91 families, 305 genera, 470 species, 1 subspecies, 30 varieties, 2 forma and total 503 taxa of herbal resources plants in Chollabuk-do area in this study. Among them 45 taxa belong to Compositae, 30 taxa belong to Rosaceae and 23 taxa belong to Leguminosae in order were occurred frequently. In general the herbal resources plants were distributed abundantly in Togyusan, Naejangsan, Changansan and Taedunsan area. Comparatively high frequent species was surveyed as follows: Schizandra chinensis, Cimicifuga heracleifolia, C. simplex, Asarum sieboldii, Arisaema amurense var. serratum, Polygonatum odoratum var. pluriflorum, Prunella vulgaris var. lilacina and Veratrum patulum etc. There was growing 30-50 individuals of Codonopsis lanceolata in 5m$\times$5m quadrat, 90 Chrysanthemum zawadskii var. latilobum plant in 1m$\times$1m and 100 Polygonatum odoratum var. pluriflorum plant in 1m$\times$1m quadrat from Togyusan quantitatively. Rare and herbal worth species were observed to distribute such as Gastrodia elata in Naejangsan and Togyusan and Acanthopanax senticosus in Mandoksan, Chinan-gun, respectively. Cultivating species in the farm now and/or favorable species in the future will be recommended here, for example, Codonopsis pilosus, A. senticosus, G. elata, Rubus coreanus, C. lanceolata and Pleuropterus multiflorus.

• Korean Journal of Environment and Ecology
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• v.24 no.2
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• pp.117-129
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• 2010

The vascular plants of the 15 sites of Taeanhaean National Park were identified as 493 taxa; 117 families, 326 genera, 436 species, 49 varieties, 6 form, 2 hybrids. The National Park area includes coastal areas and islands, these islands reflects well the floristic characteristics of coastal area and transition area of the middle province and the southern province. The study revealed that Ficus erecta Thunb., Machilus thunbergii S. et Z., Neolitsea sericea (Bl.) Koidz. and Eurya japonica Thunb. etc. were the mainly distributed species in the southern area while Rosa rugosa Thunb., Carex kobomugi Ohwi and Elymus mollis Trin., etc. were mainly distributed species over the coastal dune area. Deciduous and evergreens broad-leaved trees like Acer palmatum Thunb. ex. Murray, Euonymus japonica Thunb. and Camellia japonica L., etc. were all together distributed in Taeanhaean National Park. There are no any Endangered species designated by The Ministry of Environment were not found. The floristic special plants were recorded to a total of 53 taxa; that is, class I species (41 taxa) were containing as Vitex rotundifolia L. Fil., Arisaema heterophyllum Bl. and Arisaema ringins (Thunb.) Schott, etc, class II species (1 taxon) were Iris ensata var. spontanea (Makino) Nakai, class III species (7 taxa) were containing; Koelreuteria paniculata Laxm., Salsola collina Pall. and Daphniphyllum macropodum Miq., etc, class IV species (1 taxon) were Angelica japonica A. Gray, class V species (1 taxon) were Utricularia vulgaris var. japonica (Makino) Tamura. The naturalized plants were identified a total of 52 taxa; 17 families, 44 genera, 51 species, 1 form; Solanum carolinense L., Phytolacca americana L. and Oenothera odorata Jacq., etc. Among them the ecosystem disturbance wild animals·plants was identified as Solanum carolinense L. Hypochoeris radicata L. and Solidago altissima L. The study suggests that these areas require management such as physical remove of this ecosystem disturbance field plants.

• Korean Journal of Environment and Ecology
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• v.21 no.6
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• pp.468-486
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• 2007

It was found that vascular plants distributed at Yeondo islet were composed of 107 families, 312 genera, 408 species, 2 subspecies, 51 varieties and 5 forms, totaling 466 taxa. 39 species including Myrica rubra among the 466 taxa were evergreen broad-leaved tree, and as for flora region science-based specially designated plants by the Ministry of Environment, it was surveyed that 85 taxa(46 families, 70 genera, 80 species and 5 varieties) were distributed by identifying 53 taxa in grade one, 1 taxa in grade two, 26 taxa in grade three, 3 taxa in grade four, and Milletia japonica and Arisaema negishii in grade five. In the naturalized plants, a total of 42 taxa(13 families, 37 genera, 40 species and 2 varieties) were surveyed to be distributed and the ratio of their naturalization and Urbanization Index was analyzed to be 9.01% and 14.63%, respectively. Life form spectra bore a type of H-Dl-R5-e. Vegetation of Yeondo islet are classified as Pinus thunbergii community and Castanopsis sieboldii community, and Miscanthus sinesis var. purpurascens community spread on an gently slanted mountain side.

• Korean Journal of Environmental Biology
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• v.22 no.1
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• pp.127-132
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• 2004

The purpose of this study was to understand plant community structure in upper zone of Gyeryongsan National Payk. Total 51 plots were set up and surveyed toy this study. The fifty one plots were classified into four communities such as Quereus mongolica community, Carpinus laxiflora community, Pinus densiflora community, and Quercus variabizis community. The Quercus mongotica community were found in 29 plots of the 51 plots and the differential species in the community weve Quercus mongolica, Acer pseudosieboldianum var. koreanum, Arisaema amurense var. serratum, Diarrhena japonica, and Saussurea gyacilis. The Carpinus laxiflora community were found in 10 plots of the 51 plots and the differential species in the community were Carpinus laxiflora, Carpinus cordata, Cornus eontroversa, Carex siderosticta, and Hydrangea serrata for. acuminata. The Pinus densiflora community were found in 7 plots of the 51 plots and the differential species in the community were Pinus densiflora, Lespedeza maximowiczii, Rubus crataegifotius, Miseanthus sinensis, Persicaria filiforme, Artemisia keiskeana, and spodiopogon sibiricus. the Quereus vuriabilis community were found in 5 plots of the 51 plots and the differential species in the community were Quercus variabilis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.22 no.2
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• pp.31-48
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• 2019

The purpose of this study is to present the basic data for establishing the plan of usage and management of healing forest by surveying and analysing the vascular plants distributed in Jangseong Healing Forest. The numbers of vascular plants were summarized as 338 taxa including 85 families, 211 genera, 294 species, 3 subspecies, 33 varieties and 8 forms. In the result of life form, hemicryptophytes and geophytes were the most common and hydrophytes were the least. The rare plants were 5 taxa including Abies koreana Wilson(planted), Thuja orientalis L.(planted), Scutellaria insignis Nakai, Tricyrtis macropoda Miq. and Arisaema heterophyllum Blume. The Korean endemic plants were 12 taxa including Philadelphus schrenkii Rupr., Indigofera koreana Ohwi, Lycoris flavescens M.Y.Kim ＆ S.T.Lee and so forth. The specific plants by floristic region were 28 taxa including 2 taxa of grade IV, 3 taxa of grade III, 7 taxa of grade II and 16 taxa of grade I. The naturalized plants were 20 taxa including Fallopia convolvulus (L.) $A.L{\ddot{o}}ve$, Ailanthus altissima (Mill.) Swingle, Veronica arvensis L., Poa pratensis L. and so forth. The invasive alien plants were Rumex acetocella L. and Ambrosia artemisiifolia L..