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http://dx.doi.org/10.7732/kjpr.2018.31.1.037

Study on Molecular Phylogenetics of Korean Arisaema Species Based on Universal DNA Barcodes  

Noh, Pureum (K-herb Research Center, Korea Institute of Oriental Medicine)
Han, Kyeongsuk (K-herb Research Center, Korea Institute of Oriental Medicine)
Kim, Wook Jin (K-herb Research Center, Korea Institute of Oriental Medicine)
Yang, Sungyu (K-herb Research Center, Korea Institute of Oriental Medicine)
Choi, Goya (K-herb Research Center, Korea Institute of Oriental Medicine)
Ko, Sung Chul (Department of Biological Science and Biotechnology)
Moon, Byeong Cheol (K-herb Research Center, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Plant Resources / v.31, no.1, 2018 , pp. 37-51 More about this Journal
Abstract
Molecular phylogenetic analysis was conducted to evaluate the taxonomic relationships of genus Arisaema L. distributed in Korea and the molecular phylogenetic characteristics of three authentic Arisaema species for the herbal medicine Arisaematis Rhizoma (the rhizomes of A. amurense, A. heterophyllum, and A. erubescens). The sequences of three DNA barcodes (rDNA-ITS, matK, and rbcL) were analyzed using 50 samples of nine taxa consisted of eight Korean and one Chinese Arisaema with one outgroup (Dracunculus vulgaris). Both individual and combined phylogenetic analyses of three DNA barcode sequences revealed that the treated nine taxa are independently classified into six distinct clades (Clade I, A. amurense f. amurense and A. amurense f. serratum; Clade II, A. serratum and A. takesimense; Clade III, A. ringens; Clade IV, A. erubescens; Clade V, A. heterophyllum; Clade VI, A. thunbergii subsp. thunbergii and A. thunbergii subsp. geomundoense). These six clades were reasonably divided into three individual sections, Pedatisecta, Sinarisaema, and Tortuosa. Futhermore, the results of comparative DNA barcode sequences analyses provided a significant information for the taxonomic reconsideration of Arisaema L. at the specific and intraspecific level. However, we could not confirm the taxonomic characteristics or identity among the three authentic medicinal species through the molecular phylogenetic analyses of genus Arisaema L. for Arisaematis Rhizoma.
Keywords
Arisaema; DNA barcode; matK; Molecular phylogenetic analysis; rDNA-ITS; rbcL;
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