• 제목/요약/키워드: Arachis

검색결과 108건 처리시간 0.031초

Oil content and Fatty Acids Composition of 83 Groundnut (Arachis hypogaea L.) Genetic resources

  • Jae Eun Lee;Awraris Derbie Assefa;Young Jee Kim;Ae jin Hwang;Bich Saem Kim
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2020년도 춘계학술대회
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    • pp.95-95
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    • 2020

  • The groundnut (Arachis hypogaea L.), also known as peanut, is a high amount of good-quality vegetable oil in seeds cultivated worldwide. The aim of this study was to evaluate and analyze oil composition of 83 groundnut accessions collected from 7 countries include Korea and Philippines. Total oil contents were recovered by Soxhlet extraction and the fatty acid compositions were analyzed by using gas chromatography. Total oil contents of 83 groundnut accessions showed a significant variability among the entire domain of collections and rangerd from 44.59 to 57.58 %. The averages of palmitic and stearic acid were 11.36 and 3.39 % ranged from 8.77 to 13.65 %, and 1.88 to 7.22 %, respectively. Oleic and linoleic acid showed a wide variation which ranged from 40.32 to 61.67 %, and 22.54 to 41.99 %, respectively. Arachidic acid was ranged from 0.93 to 2.42 %. Significant negative correlation was observed between oleic and linoleic acid.

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The overexpression of Arachis hypogaea resveratrol synthase 3 (AhRS3) modified the expression pattern of phenylpropanoid pathway genes in developing rice seeds

  • Lee, Choonseok;Jeong, Namhee;Kim, Dool-Yi;Ok, Hyun-Choong;Choi, Man-Soo;Park, Ki-Do;Kim, Jaehyun
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.167-167
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    • 2017

  • Our previous study for developing seeds of Iksan 526 (I.526), an inbred line of resveratrol-producing transgenic rice line, showed that, in 20 days after heading (DAH) seeds, resveratrol was almost saturated and accumulation of piceid was highest though the expression of Arachis hypogaea resveratrol synthase 3 (AhRS3, GenBank DQ124938) was highest in 31 DAH seeds. In this study, it was investigated how the overexpression of AhRS3 affects phenylpropanoid pathway genes. p-Coumaroyl-CoA is derived from phenylpropanoid pathway and used as a substrate of AhRS3 reaction for resveratrol production. In 6, 13, 20, 31 and 41 (45 for Dongjin) DAH seeds of I526 and Dongjin, a wild type of I.526, respectively, the expression pattern of phenylpropanoid pathway genes, including phenylalanine ammonia-lyase (PAL: LOC_Os02g41630.2, LOC_Os04g43760.1), cinnamate 4-hydroxylase (C4H: LOC_Os05g25640.1), 4-coumarate-CoA ligase (4CL: LOC_Os02g08100.1), cinnamoyl-CoA reductase (CCR: LOC_ Os09g25150.1, LOC_Os08g34280.1), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: LOC_Os04g42250.2, LOC_Os02g39850.1) and cinnamyl alcohol dehydrogenase (CAD: LOC_Os02g09490.1), was examined using real time (RT)-PCR. Compared to developing seeds of Dongjin, RT-PCR results showed that the expression pattern of phenylpropanoid pathway genes was modified in developing seeds of I.526. In most genes, except for CAD, of I.526 developing seeds, the gene expression was highest in 20 DAH corresponding to biosynthesis of resveratrol and piceid, i.e. the expression of phenylpropanoid pathway genes was gradually increased by 20 DAH and decreased as seeds develop. Especially, in Dongjin, the highest expression of PALs and 4CL was in 6 DAH and their expression was gradually decreased as seeds develop. These genes expression data also exhibited that, in developing seeds of I.526, phenylpropanoid pathway genes were slightly or significantly (in some genes) upregulated compared to Dongjin. Therefore, the overexpression of AhRS3 changed the expression pattern of phenylpropanoid pathway genes in I.526 developing seeds and this modification for gene expression is closely related to biosynthesis of resveratrol and piceid.

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The overexpression of Arachis hypogaea resveratrol synthase 3 (AhRS3) modified the expression pattern of phenylpropanoid pathway genes in developing rice seeds

  • Lee, Choonseok;Jeong, Namhee;Kim, Dool-Yi;Ok, Hyun-Choong;Choi, Man-Soo;Park, Ki-Do;Kim, Jaehyun
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.105-105
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    • 2017

  • Our previous study for developing seeds of Iksan 526 (I.526), an inbred line of resveratrol-producing transgenic rice line, showed that, in 20 days after heading (DAH) seeds, resveratrol was almost saturated and accumulation of piceid was highest though the expression of Arachis hypogaea resveratrol synthase 3 (AhRS3, GenBank DQ124938) was highest in 31 DAH seeds. In this study, it was investigated how the overexpression of AhRS3 affects phenylpropanoid pathway genes. p-Coumaroyl-CoA is derived from phenylpropanoid pathway and used as a substrate of AhRS3 reaction for resveratrol production. In 6, 13, 20, 31 and 41 (45 for Dongjin) DAH seeds of I526 and Dongjin, a wild type of I.526, respectively, the expression pattern of phenylpropanoid pathway genes, including phenylalanine ammonia-lyase (PAL: LOC_Os02g41630.2, LOC_Os04g43760.1), cinnamate 4-hydroxylase (C4H: LOC_Os05g25640.1), 4-coumarate-CoA ligase (4CL: LOC_Os02g08100.1), cinnamoyl-CoA reductase (CCR: LOC_Os09g25150.1, LOC_Os08g34280.1), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: LOC_Os04g42250.2, LOC_Os02g39850.1) and cinnamyl alcohol dehydrogenase (CAD: LOC_Os02g09490.1), was examined using real time (RT)-PCR. Compared to developing seeds of Dongjin, RT-PCR results showed that the expression pattern of phenylpropanoid pathway genes was modified in developing seeds of I.526. In most genes, except for CAD, of I.526 developing seeds, the gene expression was highest in 20 DAH corresponding to biosynthesis of resveratrol and piceid, i.e. the expression of phenylpropanoid pathway genes was gradually increased by 20 DAH and decreased as seeds develop. Especially, in Dongjin, the highest expression of PALs and 4CL was in 6 DAH and their expression was gradually decreased as seeds develop. These genes expression data also exhibited that, in developing seeds of I.526, phenylpropanoid pathway genes were slightly or significantly (in some genes) upregulated compared to Dongjin. Therefore, the overexpression of AhRS3 changed the expression pattern of phenylpropanoid pathway genes in I.526 developing seeds and this modification for gene expression is closely related to biosynthesis of resveratrol and piceid.

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미국에 땅콩 줄무늬 바이러스병 발생

  • 유기열
    • 농약과 식물보호
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    • 제6권5호
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    • pp.47-49
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    • 1985

  • 땅콩 줄무늬 바이러스(peanut stripe virus, PSV)는 땅콩의 가장 피해가 큰 병 중 하나이며, 기타 중요한 두과(대두, cowpeas, arrowhead, crimson clovers)작물에도 피해를 입힌다. 최근에 이 바이러스는 중공에서 수입한 땅콩종자 유전자원(germ plasm)에 감염되어 있다는 사실을 발견하고 미국의 식물병리학자들이 세밀한 조사시험연구를 한 결과 미국에도 이미 이 바이러스가 발생되었다고 보고하였다. 그 결과 미국 동식물검역국(Animal and Plant, Health Inspection Service)은 최근에 중공으로부터 종자용을 포함한 땅콩(Arachis species)수입을 금지하기에 이르는 등 식물검역상 중요한 문제로 대두하고 있다.

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Colletotrichum gloeosporioides에 의한 땅콩 탄저병 (Anthracnose of Peanut Caused by Colletotrichum gloeosporioides)

  • 김주희;이용훈;이왕휴
    • 한국식물병리학회지
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    • 제14권6호
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    • pp.614-617
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    • 1998

  • Anthracnose of peanut (Arachis hypogaea L.) was found in the peanut cultivating fields in Iksan, Korea in September 1997. Infected plants showed irregularly circular water soaking brown lesions. In the severe case, leaves and stems were entirely died. The causal fungus of anthracnose isolated from the diseased plants was identified as Colletotrichum gloeosporioides Penz. and its teleomorph was Glomerella cingulata (Stonem.) Spauld. & Sch. according to the criteria based on the cultural and morphological characteristics. By arificial inoculation with fungal spores on healthy peanut, anthracnose symptom was observed 15 days after inoculation.

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