• Korean Journal of Ecology and Environment
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• v.37 no.1 s.106
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• pp.130-136
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• 2004

In the present study, prey preference of juvenile fishes was examined using an experimental approach. Zooplankton composition, as a prey of the fish, was evaluated by taking into account the species as well as body size of juveniles in the aquarium. The predation of juvenile fishes is known to be an important factor in changes of zooplankton communities. In some previous studies at the regulated Nakdong River, the collapse of large cladcoerans and an increase in the rotifer population by selective predation during spring and summer were observed. This study focused on the predation of juvenile fishes such as Hyporhamphus sajori, Rhinogobius brunneus, and Opsariichtys uncirostris amurensis on zooplankton community structure in mesocosm scale experiments. These fishes selected the cladoceran Moina micrura with highest individual preference value (Manly/Chesson index)among zooplankton prey in the experimental aquarium. When the size-selective prey preferences of the juvenile fish were compared, both small (body size <2 cm) and large (body size >2cm) juveniles of O. uncirostris positively selected M. micrura. In the outdoor experimental tanks, juvenile fishes consumed the cladoceran M. micrura, resulting in an high abundance of the rotifer, Polyarthra spp. The results suggest that juvenile fish predation may play an important role in regulating the zooplankton community structure by reducing the cladoceran density and increase of rotifers in the Nakdong River during spring and summer.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.24 no.4
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• pp.492-500
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• 2014

Objectives: The purpose of this study is to estimate the applicability of regional sample collection of environmental samples. The concentration of asbestos fibers were analyzed with two devices. One was an existing commercial air sampling pump that has been proved to be accurate and exact, and the other is a remodeled pump for sample collection which was made from an electric bubble generator originally designed for aquarium fish. Samples were collected with the two devices under the same environmental conditions and collection equipment. A comparative analysis of the concentration of ambient asbestos fiber was then performed. Methods: Based on previous research, six farmhouses with asbestos fiber slate roofs known to have high concentrations of asbestos fiber were selected. Using the existing commercial air sampling pump and the remodeled electric bubble generator, four to seven samples were collected each day one meter downwind from the edge of the slate roof at high volume (about 4 L/min) and low volume (about 1.4 L/min). The analyzer responsible for sample quality control of asbestos fibers counted the number of asbestos fibers with a phase microscope. Results: The rates of flow change of the existed sampler and the remodeled pump at high volume were 0.82% and 0.17%, respectively. The rates of flow change at low volume were 3.83% and 1.09%, but there was not significant difference. The rates of flow change are within the error range (${\pm}5%$) of OSHA analyzing methods. For the high volume sampler, the average asbestos fiber concentration in the air collected by the existed sampler is 6.270 fibers/L and for the remodeled one 5.527 fibers/L, not a significant difference. For the low volume sampler, the average asbestos fiber concentration in the air collected by the existed sampler is 7.755 fibers/L and for the remodeled one 7.706 fibers/L, not a significant difference. The total area of the slate roof of the targeted farmhouse has an effect on the concentration of asbestos fibers in the air from the existing pump and the remodeled one (p<0.01). Conclusions: The sampling function between the existing commercial pump and the remodeled one shows little difference. Therefore, the remodeled pump is considered a pump with a good availability for collecting ambient air asbestos samples.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.19 no.2
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• pp.164-168
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• 2014

To determine the optimal storage method and longest possible storage period of Zostera marina seeds, we examined post-storage germination rates using different storage methods and periods for Z. marina seeds harvested in southern coast of Korea. Storage methods included in seawater at room temperature with air supply, seawater at $4^{\circ}C$, seawater at $30^{\circ}C$, an aquarium with continuous seawater circulation, seawater at room temperature in an airtight tank, a refrigerator at $-20^{\circ}C$, and a desiccator at room temperature. Germination rates of Z. marina seeds stored in seawater at $4^{\circ}C$ showed highest germination (52.0%), whereas those of seeds stored in seawater at room temperature and an aquarium were 27.4% and 17.7%, respectively. But the seeds stored in seawater at $30^{\circ}C$, a refrigerator, and a desiccator did not germinate. Storage periods were 10, 20, 30, 60, 180 and 240 days of storage. Z. marina seeds maintained germination rates of 46.4~52.4% until 10~60 days of storage, but showed rapidly decreasing germination rates after then and no germination after 240 days.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.6
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• pp.604-608
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• 2009

This study was conducted to evaluate extruded pellet (EP) diet compared with a raw fish moist pellet (MP) diet for flounder, Paralichthys olivaceus on field feeding experiments in Jeju area. The experimental EP diet (juvenile, growing and adult) was prepared based on the former studies on nutrient requirements and feed ingredient of flounder. Fish were distributed randomly to each aquarium as a group of 102,300 fish (initial mean weight 97 g) in field experiment I and reared randomly to each aquarium as a group of 40,000 fish (initial mean weight 120 g) in field experiment II. In field feeding experiment I conducted in commercial flounder farm, survival, wt. gain (94-1,090 g), feed efficiency and condition factor of fish fed experimental EP were comparable to those of fish fed MP, but considerably higher protein efficiency ratio were observed in fish fed experimental EP. In field feeding experiment II, no significant difference was observed in final mean weight of fish fed the experimental EP (1,234 g) and MP (1,286 g), and any noticeable problem caused by feeding the experimental EP was not found during the whole period of feeding trial. Survival of fish fed the experimental EP was lower than that of MP, and there was no significant difference in feed efficiency between the fish fed experimental EP and MP. Therefore, these results strongly suggest that diet EP could be developed to replace MP diet for the marketable size (1.3 kg) of production for flounder without adverse effects on growth performance. In particular, abdominal dropsy and reduction of condition factor, which are frequently occurred in commercial EP feeding farms, were not observed in flounder fed experimental EP.

• Journal of Aquaculture
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• v.13 no.4
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• pp.331-338
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• 2000

To establish an alternate technology for all male seed production of Nile tilapia, Oreochromis niloticus at hatchery scale, the performance of progenies produced by crossing superfemale with sex reversed male was evaluated. Free crossings between 21 superfemales and 7 sex reversed males stocked in an aquarium for 60 days resulted in the production of 93.5 % male progenies, compared to 53.2 % males produced by an equal number of normal females and normal males. Spawning frequency was lower (19 times) in the experimental cross including superfemales than that (24 times) in the control including normal females. Seed production in the experimental aquarium was (3,085) significantly lower than that (3,797) of the control. Both in terms of seeds per female per day or seeds per g female per day, seed productivity did not significantly differ between these groups. The alternate technology involving crossing between superfemale and sex reversed male has proven to be an efficient alternate technology for mass production of all male seeds at the hatchery scale.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.25 no.2
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• pp.26-41
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• 2020

In order to culture a life for the physiological and ecological research of the meiofauna, this study aimed to identify the most ideal condition in which the meiofauna can be cultured within a laboratory by setting various environmental conditions. The sediment deposits and seawater were collected from the intertidal zone in Mallipo of the west coast. A aquarium in which the internal environment can be controlled by constantly maintaining the temperature and humidity was fabricated and the culture experiments of the collected meiofauna were conducted together with the sea water and sediment deposits collected. The experiment 1 was conducted after establishing the similar environment as the collecting location. Under the same condition as the experiment 1, the experiment 2 verified a difference between when live foods were supplied and were not. In the experiment 3, the changes in the meiofauna colony were checked according to with or without light and live foods. In the results of culturing experiments, the habitat density and the number of appeared classification groups of the meiofauna colony were relatively higher both in the water tank with supplying the live foods and under the condition of having light in 12-hour cycle than those in the aquarium without live foods and under no light condition. In addition, the habitat density of meiofauna cultured within a laboratory exhibited relatively higher value than that under the natural state.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.14 no.2
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• pp.57-62
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• 1978

When fisherman use the boat seine net to catch anchovy, a large noise (drum can, small drum and small gong) is used to scare the anchovy school along the wing nets, and into the bag net were they are caught. We want to know how much of an effect these s:mnds have on forceing the anchovy school towards the bag net. The underwater sounds of ancho\'y, drum can, small drum and small gong were analyzed in the labroatory. The behavioral responeses to the playback sounds of anchovy feeding and sounds of artificial instruments were also investigated. The feeding and artificial sounds of the samples were recorded by a tape recorder through a hydrophone in an anechoic aquarium. The sound intensity level was measured by means of a sound level meter in an anechoic chamber. The frequency and intensity of various sounds were analyzed with an analyzing system consisting of a ~-octave filter set, a high speed level recorder, an amplifier and an oscilloscope. The most successful recording was edited into a 9 to 10 second sound track and was repeated in a sequence of 9 to 10 second intervals. The sequence was then reproduced into an anechoic aquarium through the underwater speaker. The results of investigation are as follows; 1. The frequency of the feeding sound was 63~80Hz, and the pressure level produced was less than 32db. 2. The frequencies of the artificial sounds were 315~ 1,OOOHz, and the pressure levels were 88~95 db in the air. 3. When a hydrophone was placed 70cm below the surface with artificial sounds (drum can, small drum and small gong) produced 1 meter above the surface, the pressure level decreased about 30db. 4. The feeding sound was ineffective in attracting the anchovy, because of interference from ambient noise. 5. The artificial sounds had such a small effect on the anchovy's that they could not be used in ocean fisheries.

• Journal of Veterinary Clinics
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• v.27 no.3
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• pp.289-294
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• 2010

Mandarin fish (SSOGARI: Korean name) has emerged as new aquaculture and aquarium fish among Korean native fish species. Unfortunately, this fish could not exempt from infectious agent invasion. As an epidemiological survey, high mortality of mandarin fish (Siniperca scherzeri) observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida (typical A. salmonicida) using API 20E and 20NE system. However, the polymerase chain reaction (PCR) assay using two different primers (AP and MIY) revealed that the bacterial strains were not typical A. salmonicida, but atypical A. salmonicida. This study demonstrates that PCR assay is a reliable method for the confirmation of atypical A. salmonicida in mandarin fish as compared to biochemical tests. Furthermore, this is the first account of extensive dermatitis in mandarin fish due to atypical A. salmonicida infection, which has high potential in aquaculture and aquarium fish among native fish species.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.15 no.3
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• pp.183-191
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• 2005

This study is designed to compare the performance of established samplers (personal air sampler and MiniVOL portable air sampler) commonly used in the air environment or work environment with that of the sampler made by remodeling the air bubble generator for aquarium fishes. Sampling method used in this study is the filter collection method for PM10 and total suspended particles (TSP), the liquid collection method for sulfur dioxide ($SO_2$) and nitrogen dioxide ($NO_2$), and the solid collection method for toluene, respectively. There is not a significant difference in the average concentration of TSP between the Gilian personal air sampler (1st, $0.316{\pm}0.095$; 2nd $0.191{\pm}0.090$; 3rd, $0.185{\pm}0.073mg/m^3$) and the remodeled sampler (1st, $0.317{\pm}0.106$, 2nd $0.201{\pm}0.050$; 3rd $0.189{\pm}0.081mg/m^3$). There are also not significant differences in the average concentration of PM10 among the Gilian personal air sampler ($0.058{\pm}0.006mg/m^3$), the remodeled sampler ($0.052{\pm}0.008mg/m^3$) and the MiniVOL portable air sampler ($0.054{\pm}0.007mg/m^3$). The average concentration of the SO2 by the established sampler and the remodeled one is $3.79{\pm}0.21ppb$ and $3.45{\pm}0.15ppb$, respectively. In addition, there are not sigmficant differences in the average concentration of the NO2 between the Gilian personal air sampler (1st, $0.325{\pm}0.068$; 2nd $0.341{\pm}0.206$; 3rd, $2.971{\pm}0.078{\mu}g/m^3$) and the remodeled sampler (1st, $0.300{\pm}0.062$; 2nd $0.332{\pm}0.144$, 3rd, $2.968{\pm}0.085{\mu}g/m^3$). There are not significant differences in the average concentration of toluene between the Gilian personal air sampler (1st, $0.499{\pm}0.072$; 2nd $0.598{\pm}0.112$; 3rd $2.284{\pm}0.077{\mu}g/m^3$) and the remodeled sampler (1st, $0.463{\pm}0.133$; 2nd $0.603{\pm}0.082$; 3rd $2.353{\pm}0.115{\mu}g/m^3$). From these results, we can conclude that the performance of the remodeled sampler is not different from that of established samplers. There is possibility that the remodeled sampler can be used as a alternative device for Gilian personal air sampler in area and personal air sampling.

• Korean Journal of Ecology and Environment
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• v.33 no.3 s.91
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• pp.282-294
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• 2000

The reproductive ecology and the early life history of Macropodus chinensis were investigated in aquarium. Mature male made the bubble nest and spawned with wrapping the female and reverse posture. The parental male protected the offspring until the larvae depart the bubble nest. Egg productivity and egg hatching rate were the highest at water temperature in $28^{\circ}C$ and $26^{\circ}C$ respectivity than any other artificial temperature. The eggs were buoyant, globular and 0.95${\sim}$1.05 mm in diameter. Cleavage was progressed at intervals of 15 minutes. Eggs hatched in 42${\sim}$44hours after fertilization and the newly hatched larvae were 3.0${\sim}$3.2 mm in total length. The lawae were 4.5${\sim}$5.4 mm in 4${\sim}$5 days after hatching and fed on the food with dispersion from the nest. In 40${\sim}$45 days after hatching, all fin rays completely developed, and juveniles reached 18.2${\sim}$23.5 mm in total length. In 90${\sim}$110 days after hatching, body from of young fishes were similar to adult with 37.4${\sim}$48.2 mm and represented secondary sexual characters longer than 45.0 mm in total length, and about 120 days, fishes began spawning(water temperature for ontogenesis: $26.0{\pm} 1^{\circ}C$).