• 한국식생활문화학회지
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• 제32권6호
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• pp.583-588
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• 2017

This study examined the antioxidant and neuronal cell protective effects of the water and methanol extracts of Eugenia caryophyllata Thunb. The total polyphenol content was significantly higher in the methanol extract than in the water extract. The DPPH radical scavenging activity in the water extract was similar to Vit. C at a concentration of $100{\sim}200{\mu}g/mL$. The ABTS radical scavenging activity in the water and methanol extract was similar to Vit. C at a concentration of $800{\sim}1,000{\mu}g/mL$. The superoxide dismutase (SOD)-like activity in the methanol extract was similar to Vit. C at a concentration of $800{\sim}1,000{\mu}g/mL$. The DPPH, ABTS radical scavenging and (SOD)-like activity increased with increasing extract concentration. In a cell viability using MTT, the water extract (50 and 100 ppm) and methanol extract (100 ppm) had a protective effect against $H_2O_2$-induced neurotoxicity.The result ssuggest that the extract of E. caryophyllata Thunb. has antioxidant activities and may be useful for treating neurodegenerative disorders.

• 한국축산식품학회지
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• 제38권6호
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• pp.1168-1178
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• 2018

The present study aimed to characterise anti-oxidant peptides from water-soluble protein extracts of Hanwoo beef and evaluate their anti-proliferative effect on human colorectal carcinoma cells (HCT116). Antioxidant peptides were purified from the low-molecular-weight fraction (<3 kDa) of Hanwoo beef extract. Antioxidant activity of peptide fractions was determined using the oxygen radical absorbance capacity (ORAC) assay. Purified peptide (P3) displayed higher ORAC activity than the low-molecular-weight fraction ($202.66{\mu}M\;TE/g$ vs $167.38{\mu}M\;TE/g$ of dry matter, respectively) (p<0.05). The peptide sequence of P3 was Cys-Cys-Cys-Cys-Ser-Val-Gln-Lys (888.30 Da). The novel peptide P3, at $250{\mu}g/mL$, also significantly inhibited HCT116 cell proliferation up to 25.24% through phosphorylation of ERK, JNK, and p38 kinase (p<0.05). Hence, antioxidant peptide P3 from Hanwoo beef extract can be used as an antioxidative and anticancer agent in the functional food industry.

• 한국식생활문화학회지
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• 제35권6호
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• pp.605-612
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• 2020

In this study, 11 plant extracts from Bangladesh were used to evaluate the total phenolic and flavonoid content, in vitro antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzthiazolin-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay. Also, the inhibitory effect of nitric oxide (NO) production in RAW 264.7 macrophage cell line and the neuroprotective effect on H2O2-induced PC12 cells were tested. Our results revealed that Piper betle L. showed the highest total phenolic content (162.2 mg GAE/g extract) among the 11 plants from Bangladesh. Most plants showed strong radical scavenging effects and ferric reducing antioxidant power. Besides, Piper betle L. protected PC12 neuronal cells against H2O2 related oxidative stress in LPS-induced PC12 cells. Regarding the anti-inflammatory effect, Piper betle L. significantly inhibited NO accumulation in LPS-induced RAW 264.7 cells. Our results provide evidence that Piper betle L. could be useful for the development of functional health foods.

• 한국약용작물학회지
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• 제14권6호
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• pp.322-328
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• 2006

Green tea is of particular source as it has been found to have strong antioxidant activities. The extracts of green tea during the commercial harvest seasons from April, 2003 to August, 2003 were compared. The purpose of this study was to determine the correlation between the polyphenol content of green teas and its antioxidant activities. The con-tent of total polyphenols was analyzed and several antioxidant testings were performed. The levels of total polyphenols were higher in the green teas (e.g. Woojeon, Sejak) harvested during very early spring and lower in the green teas harvested late(eg. Ipha, Yepcha). In particular, the free radical scavenging, the inhibition of LDL oxidation, the cytoprotective effect and the inhibition of DNA damage were correlated with the total polyphenol contents of green tea extracts harvested early spring such as Woojeon, Sejak and Jungjak. The results obtained here show that all extracts of green teas including purified green tea catechin, GTC, have strong antioxidant activities on oxidative stress in vifrθ. The variation in polyphenol content and antioxidant activities among various types of green tea by the harvesting time may provide critical information for investigators and consumers using tea in purposes of nutrition and chemoprevention.

• 대한한방내과학회지
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• 제30권4호
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• pp.746-760
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• 2009

Objectives : This study was designed to evaluate the effect of Hasuogamibang (HGB) on hyperlipidemia and antioxidant activity. Methods : For this study, we divided four groups of rats (normal WKY group, normal SHR group, high cholesterol diet and saline-treated SHR group, high cholesterol diet and HGB-treated SHR group), and observed the change of body weight, weight of liver, cholesterol, triglyceride, glucose, albumin, histologic change of liver and aorta, lipid peroxidation and antioxidant activity of liver tissue, and cholesterol gene revelation control efficiency. Results : Total-cholesterol, LDL-cholesterol, triglycerides were decreased significantly by HGB. However, HDL-cholesterol increased significantly. The tissue of liver and aorta were controlled defect by HGB on histologic study. Lipid peroxidation and SOD of liver tissue was decreased significantly by HGB. Gene revelation of ACAT and HMG CoA reductase in hepatic tissue was decreased significantly by HGB. Conclusion : This study suggests that HOB is significantly effective on hyperlipidemia and antioxidant activity.

• Journal of Nutrition and Health
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• 제31권3호
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• pp.289-296
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• 1998

A large body of epidemiologic evidence suggests inverse relationships between ischemic heart disease and plasma vitamin C and E concentrations. Smokers have lower plasma concentrations of these vitamins than do nonsmokers. Smokers therefore need antioxidant vitamin supplementation . The purpose of study was to investigate the effect of antioxidant vitamin supplementation on blood composition in smoking college men. 24 subjects were divided into 3 groups of which were the vitamin C supplementation group(n=8), the vitamin E supplementation group(n=8), and the vitamin C+E supplementation group(n=8). The vitamin supplementation group consumed 500mg of ascorbic acid, the vitamin E supplementation group consumed 200IU of D-$\alpha$-tocopherol , and the vitamin C+E supplementation group consumed 500mg of ascorbic acid +200IU of D-$\alpha$-tocopherol for 4 weeks. We examined the blood compositions of the volunteers bofore and after vitamins were supplemented . The results obtained were as follows ; intakes of energy , carbohydrate , fat protein , vitamin C and vitamin E were not significantly affected by vitamin supplementation in all groups. Blood glucose concentrations were not significantly affected by vitamin supplementation in all groups. Concentrations of plasma uric acid and alkaline phosphatase activity were decreased significantly (p<0.05) with vitamin E supplementation. The results of this study show that antioxidant vitamin supplementation in smokers has a tendency to decrease coronary heart disease risk.

• Journal of Nutrition and Health
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• 제37권6호
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• pp.440-447
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• 2004

In this study the in vitro protective effects of several antioxidant vitamins (vitamin C, $\alpha$-tocopherol, $\beta$-carotene), fruits and vegetables (strawberry, tangerine, orange and 100％ orange juice, carrot juice), on the levels of isolated human lymphocyte DNA damage was measured using Comet assay. Comet assay has been used widely to assess the level of the DNA damage in the individual cells. Lymphocytes were pre-treated for 30 minutes with antioxidant vitamins (10, 50, 100, 500 $\mu$M) or fruits$.$vegetables (10, 100, 500, 1000 $\mu$g/ml), an4 then oxidatively challenged with 100 $\mu$M $H_2O$$_2$ for 5 min at 4$^{\circ}C$. The protective effect of antioxidant vitamins against DNA damage at a concentration of 50 $\mu$M were 50％ in vitamin C, 32％ in $\alpha$-tocopherol, whereas, fJ-carotene showed a 55％ protection at a dose as low as 10 $\mu$M. The inhibitory effects of DNA damage by strawberry, tangerine, orange, orange juices, carrot juices were 50 - 60％ with wide ranges of doses. The results of the present study indicate that most the antioxidant vitamins and fruits$.$vegetables juices produced a significant reduction in oxidative DNA damage.

• Advances in Traditional Medicine
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• 제7권4호
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• pp.426-435
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• 2007

The present study was designed to determine the antitumor and antioxidant properties of methanol extract from the root of Galega purpurea (Papilionaceae) (MEGP) against Ehrlich Ascites Carcinoma (EAC) bearing Swiss albino mice. Acute and short-term toxicity studies were performed initially in order to ascertain the safety of MEGP. The effect of MEGP on the growth of transplantable murine tumor, life span of EAC bearing hosts and simultaneous alterations in the hematological profile and liver biochemical parameters (lipid peroxidation, antioxidant enzymes) were estimated. The MEGP showed decrease in tumor volume, packed cell volume and viable cell count and increases the nonviable cell count and mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to more or less normal levels in extract treated mice. Treatment with MEGP decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase. The results suggested that the methanol extract of Galega purpurea root exhibited antitumor effect by modulating lipid peroxidation and augmenting antioxidant defense system in EAC bearing mice.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.210-210
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• 2017

Sorghum is rich source of various phytochemicals including phenolic acids that have potential to significantly impact human health. Phytochemical production may be induced by not only genotype but a number of environmental factors including temperatures and amount of sunshine. The objective of this study was to determine the effect of planting date and harvesting stage on the agronomic and quality traits of 'Donganme' grain sorghum variety developed to produce high antioxidant activity. 'Donganme' were planted in three locations at four dates from early May to early July. Each planted fractions were harvested five times 35, 40, 45, 50, 55 days after head shooting date, respectively. Significant difference existed between the growth period and the agronomic traits. The interaction effects planting date and harvesting date was significant for plant height, tiller production, grain yield and antioxidant activity, indicating that low temperature and integrated sunshine influence on that traits. The result showed that antioxidant activity production occurred when the sorghum crop was grown in late season although the yield is lower. To produce antioxidant activity from sorghum grain need to consider the relation between the yield and nutrition component simultaneously.

• Asian-Australasian Journal of Animal Sciences
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• 제21권6호
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• pp.890-895
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• 2008

The antioxidant activity of resveratrol in cholesterol-fed rats, along with its hypolipidemic effects was determined. Thirty two male Sprague-Dawley (SD) rats were randomly divided into three groups (Control, Res30 and Res70) and fed a hyperlipidemic diet for 4 weeks. Resveratrol was suspended in 0.3% carboxymethyl cellulose (CMC) solution and given to rats of the Res30 and Res70 groups once a day for 4 weeks by oral intubation at a dose of 30 and 70 mg/kg body weight, respectively. The control group received 0.3% CMC solution alone. Resveratrol significantly lowered serum lipid, hepatic cholesterol (TC) and triglyceride (TG) levels compared to the control. Excretion of bile acids was significantly enhanced by resveratrol. The overall potential of the antioxidant system was significantly enhanced by the resveratrol as plasma and hepatic thiobarbituric acid reactive substances (TBARS) levels were lowered while serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased in the cholesterol-fed rats. These findings suggest that resveratrol maintains an antioxidant efficacy as well as its anti-hyperlipidemic effect.