Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Comparison of the Protective Effect of Antioxidant Vitamins and Fruits or Vegetable Juices on DNA Damage in Human Lymphocyte Cells Using the Comet Assay

Comet Assay를 이용한 항산화 비타민과 과일.야채즙의 인체 임파구 세포 DNA 손상 감소 효과 비교

  • 전은재 (한남대학교 이과대학 식품영양학과) ;
  • 박유경 (한남대학교 이과대학 식품영양학) ;
  • 김정신 (한남대학교 이과대학 식품영양학) ;
  • 강명희 (한남대학교 이과대학 식품영양학과)
  • Published : 2004.07.01
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Abstract

In this study the in vitro protective effects of several antioxidant vitamins (vitamin C, $\alpha$-tocopherol, $\beta$-carotene), fruits and vegetables (strawberry, tangerine, orange and 100％ orange juice, carrot juice), on the levels of isolated human lymphocyte DNA damage was measured using Comet assay. Comet assay has been used widely to assess the level of the DNA damage in the individual cells. Lymphocytes were pre-treated for 30 minutes with antioxidant vitamins (10, 50, 100, 500 $\mu$M) or fruits$.$vegetables (10, 100, 500, 1000 $\mu$g/ml), an4 then oxidatively challenged with 100 $\mu$M $H_2O$$_2$ for 5 min at 4$^{\circ}C$. The protective effect of antioxidant vitamins against DNA damage at a concentration of 50 $\mu$M were 50％ in vitamin C, 32％ in $\alpha$-tocopherol, whereas, fJ-carotene showed a 55％ protection at a dose as low as 10 $\mu$M. The inhibitory effects of DNA damage by strawberry, tangerine, orange, orange juices, carrot juices were 50 - 60％ with wide ranges of doses. The results of the present study indicate that most the antioxidant vitamins and fruits$.$vegetables juices produced a significant reduction in oxidative DNA damage.

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References

  1. Chem Res Toxicol v.12 no.6 Reactions of betacarotene with cigarette smoke oxidants. Identification of carotenoid oxidation products and evaluation of the prooxidant/antioxidant effect Baker DL;Krol ES;Jacobsen N;Liebler DC https://doi.org/10.1021/tx980263v
  2. Free Radic Biol Med v.27 Vitamin C prevents cigarette smoke induced oxidative damage of proteins and increased proteolysis Panda K;Chattopadhyay R;Ghosh MK;Chattopadhyay DJ;Chatterjee IB https://doi.org/10.1016/S0891-5849(99)00154-9
  3. Nutr Cancer v.34 Dietary antioxidant supplementation and DNA damage in smokers and nonsmokers Welch RW;Turley E;Sweetman SF;Kennedy G;Collins AR;Dunne A;Livingstone MB;McKenna PG;McKelvey-Martin VJ;Strain JJ https://doi.org/10.1207/S15327914NC3402_7
  4. Cancer Lett v.132 Inhibition of oxidative DNA damage, 8-OHdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, beta-carotene and red ginseng) Lee BM;Lee SK;Kim HS https://doi.org/10.1016/S0304-3835(98)00227-4
  5. Toxicol in Vitro v.15 no.1 Antioxidant and prooxidant roles for betacarotene, alpha-tocopherol and ascorbic acid in human lung cells Zhang P;Omaye ST https://doi.org/10.1016/S0887-2333(00)00054-0
  6. Am J Clin Nutr v.69 no.4 Does tomato consumption effectively increase the resistance of lymphocyte DNA to oxidative damage? Riso P;Pinder A;Santagelo A; Porrini M https://doi.org/10.1093/ajcn/69.4.712
  7. Carcinogenesis v.18 no.9 Consumption of vegetables reduces genetic damage in humans:first results of a human intervention trial with carotenoid-rich foos Pool-Zobel BL;Bub A;Muller H;Wollowski L;Rechkemmer G https://doi.org/10.1093/carcin/18.9.1847
  8. Methods Enzymol v.213 Antioxidant effects of carotenoids in vivo and in vitro:an overview Palozza P;Krinsky NI https://doi.org/10.1016/0076-6879(92)13142-K
  9. Physiol Rev v.74 no.1 Cellular defenses against damage from reactive oxygen species Yu BP https://doi.org/10.1152/physrev.1994.74.1.139
  10. Korean J Nutrition v.35 no.2 Application of the alkaline comet assay for detecting oxidative DNA damage in human biomonitoring Park EJ;Kang MH
  11. Mutat Res v.393 Quercetin and myricetin protect against hydrogen peroxide-induced DNA damage (stand breaks and oxidised pyrimidines) in human lymphocytes Duthie SJ;Collins AR;Duthie GG;Dobson VL https://doi.org/10.1016/S1383-5718(97)00107-1
  12. Mutat Res v.390 The effect of dietary flavonoids on DNA damage (strand breaks and oxidised pyrimidines) and growth in human cells Duthie SJ;Johnson W;Dobson VL https://doi.org/10.1016/S0165-1218(97)00010-4
  13. Am J Clin Nutr v.67 Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes Noroozi M;Angerson WJ;Lean MEJ https://doi.org/10.1093/ajcn/67.6.1210
  14. Food Chemical Toxicol v.39 Effects of brussel sprouts extracts on hydrogen peroxide-induced DNA strand breaks in human lymphocytes Zhu CY;Loft S https://doi.org/10.1016/S0278-6915(01)00061-8
  15. J Korean Soc Food Sci Nutr v.29 no.2 The antigenotoxic effects of Korean native fermented food, beachu kimchi using comet assay Ji ST;Park JH;Hyun CK;Shin HK
  16. Korean J Nutrition v.36 no.2 Protective effect of flavonoids on lymphocyte DNA damage using comet assay Park YK;Jeon EJ;Kang MH
  17. Korean J Nutrition v.36 no.1 Protective effect of yellow-green vegetable juices on DNA damage in chinese hamster lung cell using comet assay Jeon EJ;Kim JS;Park YK;Kim TS;Kang MH
  18. Free Rad Res v.27 Ex vivo assessment of lymphocyte antioxidant status using the comet assay Panayiotidis M;Collins AR https://doi.org/10.3109/10715769709065793
  19. Free Rad Res v.36 no.1 Effects of dietary antioxidants on human DNA ex vivo Szeto YT;Benzie IFF https://doi.org/10.1080/10715760210161
  20. Mutat Res v.500 Effects of dietary antioxidants on DNA damage in lysed cells using a modified comet assay procedure Szeto YT;Collins AR;Benzie IFF https://doi.org/10.1016/S0027-5107(01)00298-6
  21. FEBS v.449 ${\beta}$-Carotene engances hydrogen peroxide-induced DNA damage in human hepatocellular HepG2 cells Woods JA;Bilton RF;Young AJ https://doi.org/10.1016/S0014-5793(99)00450-0
  22. Nutr Cancer v.43 no.3 Increased cellular carotenoid levels reduce the persistence of DNA single-strand breaks after oxidative challenge Astley SB;Elliott RM;Archer DB https://doi.org/10.1207/S15327914NC432_11
  23. J Nutr Biochem v.13 no.10 Flavonoid antioxidants:chemistry, metabolism and structure-activity relationships Heim KE;Tagliaferro AR;Bobilya DJ https://doi.org/10.1016/S0955-2863(02)00208-5