Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Resveratrol on Serum and Liver Lipid Profile and Antioxidant Activity in Hyperlipidemia Rats

  • Zhu, Lixian (College of Food Science and Technology, Shandong Agricultural University) ;
  • Luo, Xin (College of Food Science and Technology, Shandong Agricultural University) ;
  • Jin, Zhengyu (College of Food Science and Technology, Southern Yangtze University)
  • Received : 2007.10.20
  • Accepted : 2008.01.03
  • Published : 2008.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidant activity of resveratrol in cholesterol-fed rats, along with its hypolipidemic effects was determined. Thirty two male Sprague-Dawley (SD) rats were randomly divided into three groups (Control, Res30 and Res70) and fed a hyperlipidemic diet for 4 weeks. Resveratrol was suspended in 0.3% carboxymethyl cellulose (CMC) solution and given to rats of the Res30 and Res70 groups once a day for 4 weeks by oral intubation at a dose of 30 and 70 mg/kg body weight, respectively. The control group received 0.3% CMC solution alone. Resveratrol significantly lowered serum lipid, hepatic cholesterol (TC) and triglyceride (TG) levels compared to the control. Excretion of bile acids was significantly enhanced by resveratrol. The overall potential of the antioxidant system was significantly enhanced by the resveratrol as plasma and hepatic thiobarbituric acid reactive substances (TBARS) levels were lowered while serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) activities were increased in the cholesterol-fed rats. These findings suggest that resveratrol maintains an antioxidant efficacy as well as its anti-hyperlipidemic effect.

Keywords

References

  1. AI-Manun, M., C. Tanaka, Y. Hanai, Y. Tamura and H. Sano. 2007. Effects of Plantain (Plantago lanceolata L.) herb and heat exposure on plasma glucose metabolism in sheep. Asian-Aust. J. Anim. Sci. 20(6):894-899. https://doi.org/10.5713/ajas.2007.894
  2. Arichi, H., Y. Kimura, H. Okuda, K. Baba, M. Kozawa and S. Arichi. 1982. Effects of stilbene components of the roots of Polygonum cuspidatum Sieb. et Zucc. on lipid metabolism. Chemical and Pharmaceutical Bulletin 30(5):1766-1770. https://doi.org/10.1248/cpb.30.1766
  3. Aust, S. D. and S. A. Svingen. 1982. In: Free radicals in biology (Ed. W. A. Pryor). Academic Press, New York, 1-28.
  4. Belch, J. F., A. B. Bridges, N. Scott and M. Chopra. 1991. Oxygen free radicals and congestive heart failure. Br. Heart J. 65:245-248. https://doi.org/10.1136/hrt.65.5.245
  5. Belguendouz, L., L. Fremont and M. T. Gozzelino. 1998. Interaction of transresveratrol with plasma lipoproteins. Biochem. Pharmacol. 55:811-816. https://doi.org/10.1016/S0006-2952(97)00544-3
  6. Belguendouz, L., L. Fremont and A. Linard. 1997. Resvertrol inhibits metal ion-dependent and independent peroxidation of porcine low-density lipoproteins. Biochem. Harmacol. 53: 1347-1355. https://doi.org/10.1016/S0006-2952(96)00820-9
  7. Brian, P., Laden, Todd, D. and Porter. 2001. Resveratrol inhibits human squalene monooxygenase. Nutr. Res. 21:747-753. https://doi.org/10.1016/S0271-5317(01)00287-1
  8. Carlson, L. A. 1982. Metabolic risk factors in ischemic cardiovascular disease. Raven Press, New York, 1-3.
  9. Chung, J. Y., J. H. Kim, Y. H. Ko and I. S. Jang. 2007. Effects of dietary supplemented inorganic and organic selenium on antioxidant defense systems in the intestine, serum, liver and muscle of Korean native goats. Asian-Aust. J. Anim. Sci. 20(1):52-59.
  10. Comporti, M. 1985. Lipid peroxidation and cellular damage in toxic liver injury. Laboratory Investigation 53:599-623.
  11. Daiki, M., M. Yutaka and Y. Kazumi. 2003. Hypolipidemic action of dietary resveratrol, a phytoalexin in grapes and red wine, in hepatoma-bearing rats. Life Sci. 73:1393-1400. https://doi.org/10.1016/S0024-3205(03)00469-7
  12. Dreosti, I. E. 2000. Antioxidant polyphenols in tea, cocoa, and wine. Nutr. 16:692-701. https://doi.org/10.1016/S0899-9007(00)00304-X
  13. Dvir, I., R. Chayoth, U. Sod-Moriah, S. Shany, A. Nyska and A. H. Stark. 2000. Soluble polysaccharide and biomass of red microalga Porphyridium sp. alter intestinal morphology and reduce serum cholesterol in rats. Br. J. Nutr. 84:469-476.
  14. Einarsson, K., L. Benthin, S. Ewerth, G. Hellers, D. Stahlberg and B. Angelin. 1989. Studies on acyl-coenzyme A: cholesterol acyltransferase activity in human liver microsomes. J. Lipid Res. 30:739-746.
  15. Fauconneau, B., P. Waffo-Teguo, F. Huguet, L. Barrier, A. Decendit and J. M. Merillon. 1997. Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests. Life Sci. 61(21):2103-2110. https://doi.org/10.1016/S0024-3205(97)00883-7
  16. Folch, J., M. Lees and G. H. Sloan-Stanley. 1957. A simple method for isolation, and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
  17. Frankel, E. N., A. L. Waterhouse, and J. E. Kinsella. 1993. Inhibition of human LDL oxidation by resveratrol. Lancet. 341: 1103-1104.
  18. Fremont, L. 2000. Biological effects of resveratrol. Life Sci. 66: 663-673. https://doi.org/10.1016/S0024-3205(99)00410-5
  19. Fridovich, I. 1989. Superoxide dismutases: An adaptation to a paramagnetic gas. J. Biologic. Chem. 264:7761-7764.
  20. Halliwell, B. and J. M. Gutteridge. 1990. Role of free radicals and catalytic metal ions in human disease: an overview. Methods in Enzymology 186:1-85. https://doi.org/10.1016/0076-6879(90)86093-B
  21. Hosoda, K., K. Kuramoto, B. Eruden, T. Nishida and S. Shioya. 2006. The effects of three herbs as feed supplements on blood metabolites, hormones, antioxidant activity, IgG concentration, and ruminal fermentation in holstein steers. Asian-Aust. J. Anim. Sci. 19(1):35-41.
  22. Huff, M. W. and D. E. Telford. 1989. Dietary fish oil increases the conversion of VLDL apoB to LDL apoB. Atherosclerosis 9(1):58-66.
  23. Jang, M., L. Cai, G. O. Udeani, K. V. Slowing, C. F. Thomas, C. W. Beecher, H. H. Fong, N. R. Farnsworth, A. D. Kinghorn, R. G. Mehta, R. C. Moon and J. M. Pezzuto. 1997. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Sci. 275:218-220. https://doi.org/10.1126/science.275.5297.218
  24. Kawada, N., S. Seki, M. Inoue and T. Kuroki. 1998. Effect of antioxidants, resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells. Hepatology 27(5):1265-1274. https://doi.org/10.1002/hep.510270512
  25. Krause, B. R., M. E. Pape, K. Kieft, B. Auerbach, C. L. Bisgaier, R. Homan and R. S. Newton. 1994. ACAT inhibition decreases LDL cholesterol in rabbits fed a cholesterol-free diet. Marked changes in LDL cholesterol without changes in LDL receptor mRNA abundance. Arterioscler Thromb. 14:598-604. https://doi.org/10.1161/01.ATV.14.4.598
  26. Lee, M. K., S. H. Bok, T. S. Jeong, S. S. Moon, S. E. Lee, T. B. Park and M. S. Choi. 2002. Supplementation of naringenin and its synthetic derivative alters antioxidant enzyme activities of erythrocyte and liver in high cholesterol-fed rats. Bioorganic and Medicinal Chemistry 10:2239-2244. https://doi.org/10.1016/S0968-0896(02)00059-7
  27. Miller, G. J. and N. E. Miller. 1975. Plasma high-density lipoprotein concentration and development of ischemic heart disease. Lancet. 1:16-19. https://doi.org/10.1016/S0140-6736(75)92376-4
  28. Miller, J. P. 1996. Hyperlipidaemia and cardiovascular disease. Curr. Opin. Lipidol. 7:18-24. https://doi.org/10.1097/00041433-199602000-00018
  29. Noguchi, N. and E. Niki. 2000. Phenolic antioxidants: a rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radical Biol. Med. 28(10):1538-1546. https://doi.org/10.1016/S0891-5849(00)00256-2
  30. O'Brien, B. C., C. L. Skutches, G. R. Henderson and R. Reiser. 1977. Interrelated effects of food lipids on steroid metabolism in rats. J. Nutr. 107(8):1444-1454. https://doi.org/10.1093/jn/107.8.1444
  31. Park, C. S., Y. C. Lee, J. D. Kim, H. M. Kim and C. H. Kim. 2004. Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme Acholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells. Vascular Pharmacol. 40:279-284. https://doi.org/10.1016/j.vph.2004.01.003
  32. Park, S. Y., S. H. Bok, S. M. Jeon, Y. B. Park, S. J. Lee, T. S. Jeong and M. S. Choi. 2002. Effect of rutin and tannic acid supplements on cholesterol metabolism in rats. Nutr. Res. 22: 283-295. https://doi.org/10.1016/S0271-5317(01)00398-0
  33. Rice-Evans, C. A. 1996. Structure-antioxidant relationships of flavonoids, and phenolic acids. Free Radical Biol. Med. 20: 933-956. https://doi.org/10.1016/0891-5849(95)02227-9
  34. Rotondo, S., G. Rajtar, S. Manarini, A. Celardo, D. Rotillo, G. Gaetano, V. Evangelista and C. Cerletti. 1998. Effect of transresveratrol, a natural polyphenolic compound, on human polymorphonuclear leukocyte function. Br. J. Pharmacol. 123(8):1691-1699. https://doi.org/10.1038/sj.bjp.0701784
  35. SAS Institute Inc. 1999. SAS system 8.0. SAS Institute Inc., Cary, NC, USA.
  36. Shan, C., S. Yang, H. He, S. Shao and P. P. Zhang. 1990. Cuspidatum root of traditional folk medicine for the treatment of atherosclerosis. Acta Pharmacologica Sinica 11:524-530.
  37. Shen, A. L., T. D. Porter, T. E. Wilson and C. B. Kasper. 1989. Structural analysis of the FMN binding domain of NADPH cytochrome P-450 oxidoreductase by site-directed mutagenesis. J. Biol. Chem. 264:7584-7589.
  38. Steinbrecher, U. P., H. Zhang and M. Lougheed. 1990. Role of oxidatively modified LDL in atherosclerosis. Free Radical Biolology and Medicine 9:155-168.
  39. Sun, A. Y., Y. M. Chen, M. James-Kracke, P. Wixom and Y. Cheng. 1997. Ethanol-induced cell death by lipid peroxidation in PC12 cells. Neurochem. Res. 22:1187-1192. https://doi.org/10.1023/A:1021968526696
  40. Wilson, T., T. J. Knight, D. C. Beitz, D. S. Lewis and R. L. Engen. 1996. Resveratrol promotes atherosclerosis in hypercholesterolemic rabbits. Life Sci. 59(1):15-21. https://doi.org/10.1016/0024-3205(96)00260-3
  41. Wu, X., N. Sakata, E. Lui and H. N. Ginsberg. 1994. Evidence for a lack of regulation of the assembly and secretion of apolipoprotein B-containing lipoprotein from HepG2 cells by cholesterylester. J. Biol. Chem. 269:12375-12382.
  42. Yu, B. P. 1994. Cellular defenses against damage from reactive oxygen species. Physiological Reviews 74:139-162. https://doi.org/10.1152/physrev.1994.74.1.139
  43. Yuan, H., Z. Gong, L. Y. Yuan and B. Han. 2006. In vitro arsanilic acid induction of apoptosis in rat hepatocytes. Asian-Aust. J. Anim. Sci. 19(9):1328-1334. https://doi.org/10.5713/ajas.2006.1328
  44. Yuan, S. B., D. W. Chen, K. Y. Zhang and B. Yu. 2007. Effects of oxidative stress on growth performance, nutrient digestibilities and activities of antioxidative enzymes of weanling pigs. Asian-Aust. J. Anim. Sci. 20(10):1600-1605. https://doi.org/10.5713/ajas.2007.1600
  45. Yugarani, T., B. K. Tan, M. The and N. P. Das. 1992. Effects of polyphenolic natural products on the lipid profiles of rats fed high fat diets. Lipids 27(3):181-186. https://doi.org/10.1007/BF02536175
  46. Zhang, H. Y., N. Ge and Z. Y. Zhang. 1999. Theoretical elucidation of activity differences of five phenolic antioxidants. Acta Pharmacologica Sinica 20(4):363-366.

Cited by

  1. Purification of resveratrol, arachidin-1, and arachidin-3 from hairy root cultures of peanut (Arachis hypogaea) and determination of their antioxidant activity and cytotoxicity vol.26, pp.5, 2010, https://doi.org/10.1002/btpr.454
  2. High dose of dietary resveratrol enhances insulin sensitivity in healthy rats but does not lead to metabolite concentrations effective for SIRT1 expression vol.55, pp.8, 2011, https://doi.org/10.1002/mnfr.201100292
  3. Differential effects of low-dose resveratrol on adiposity and hepatic steatosis in diet-induced obese mice vol.108, pp.12, 2012, https://doi.org/10.1017/S0007114512000347
  4. Grape Seed Extract Effects in Brain After Hypobaric Hypoxia vol.16, pp.9, 2013, https://doi.org/10.1089/jmf.2012.0196
  5. Antioxidant Effects of Spinach (Spinacia oleracea L.) Supplementation in Hyperlipidemic Rats vol.19, pp.1, 2014, https://doi.org/10.3746/pnf.2014.19.1.019
  6. Chinese Herbal Medicine on Dyslipidemia: Progress and Perspective vol.2014, pp.1741-4288, 2014, https://doi.org/10.1155/2014/163036
  7. Impact of Chicory-Supplemented Diet on HMG-CoA Reductase, Acetyl-CoA Carboxylase, Visfatin and Anti-Oxidant Status in Triton WR-1339-Induced Hyperlipidemia vol.39, pp.2, 2015, https://doi.org/10.1111/jfbc.12115
  8. Oxidative stress biomarkers in organs of hyperlipidaemic and normal rats fed tropical red seaweed, Gracilaria changii vol.28, pp.2, 2016, https://doi.org/10.1007/s10811-015-0670-x
  9. Cholesterol-Lowering Effects and Mechanisms in View of Bile Acid Pathway of Resveratrol and Resveratrol Glucuronides vol.81, pp.11, 2016, https://doi.org/10.1111/1750-3841.13528
  10. Resveratrol mitigates hypercholesterolemia exacerbated hyperthermia in chronically heat-stressed rats vol.12, pp.2, 2019, https://doi.org/10.14202/vetworld.2019.337-344
  11. Non-alcoholic steatohepatitis: an overview including treatments with herbals as alternative therapeutics vol.10, pp.3, 2012, https://doi.org/10.2478/v10136-012-0008-9
  12. Resveratrol and fenofibrate ameliorate fructose-induced nonalcoholic steatohepatitis by modulation of genes expression vol.22, pp.10, 2008, https://doi.org/10.3748/wjg.v22.i10.2931
  13. Antihyperlipidemic and Antioxidant Effects of Averrhoa Carambola Extract in High-Fat Diet-Fed Rats vol.7, pp.3, 2019, https://doi.org/10.3390/biomedicines7030072
  14. The Fluid Aspect of the Mediterranean Diet in the Prevention and Management of Cardiovascular Disease and Diabetes: The Role of Polyphenol Content in Moderate Consumption of Wine and Olive Oil vol.11, pp.11, 2008, https://doi.org/10.3390/nu11112833
  15. Long-term effects of maternal resveratrol intake during lactation on cholesterol metabolism in male rat offspring vol.71, pp.2, 2008, https://doi.org/10.1080/09637486.2019.1639638
  16. The effects of resveratrol supplementation in patients with type 2 diabetes, metabolic syndrome, and nonalcoholic fatty liver disease: an umbrella review of meta-analyses of randomized controlled tria vol.114, pp.5, 2008, https://doi.org/10.1093/ajcn/nqab250