• Title/Summary/Keyword: Antimicrobial Film

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Chitosan-Based Film of Tyrothricin for Enhanced Antimicrobial Activity against Common Skin Pathogens Including Staphylococcus aureus

  • Han, Sang Duk;Sung, Hyun Jung;Lee, Ga Hyeon;Jun, Joon-Ho;Son, Miwon;Kang, Myung Joo
    • Journal of Microbiology and Biotechnology
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    • v.26 no.5
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    • pp.953-958
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    • 2016
  • Chitosan-based film-forming gel is regarded as a promising vehicle for topical delivery of antimicrobial agents to skin wounds, since it protects from microbial infection and the cationic polymer itself possesses antibacterial activity. In this study, possible synergistic interaction against common skin pathogens between the cationic polymer and tyrothricin (TRC), a cyclic polypeptide antibiotic, was investigated, by determining the concentration to inhibit 90% of bacterial isolates (MIC). The addition of the polysaccharide to TRC dramatically reduced the MIC values of TRC by 1/33 and 1/4 against both methicillin-resistant and methicillin-susceptible Staphylococcus aureus, respectively. The synergism of TRC and chitosan combination against both strains was demonstrated by the checkerboard method, with a fractional inhibitory concentration index below 0.5. Moreover, co-treatment of TRC and chitosan exhibited antibacterial activity against Pseudomonas aeruginosa, due to the antibacterial activity of chitosan, whereas TRC itself did not inhibit the gram-negative bacterial growth. These findings suggested that the use of chitosan-based film for topical delivery of TRC could be an alternative to improve TRC antimicrobial activity against strains that are abundant in skin wounds.

Development of Thin, Transparent Oxo-Biodegradable Film with Antibacterial and Freshness Agent (항균, 신선도 기능을 부여한 투명 산화생분해 필름 개발)

  • Choi, Sung-Wook;Lee, Kun-Woo;Yu, Ji-Ye;You, Young-Sun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.23 no.3
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    • pp.133-141
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    • 2017
  • This article described the development of transparent antimicrobial oxo-biodegradable (AOB) film with the function of enhanced freshness of food by employing oxo-biodegradable masterbatch (MB) and antimicrobial MB together with organic metal salt, organic acid, or unsaturated fatty acid. Antibacterial test of the AOB film with the different contents of the antimicrobial MB resulted in the significant freshness extension of plum. Tensile strength and elongation rate of the AOB films before UV treatment were similar to those of polyethylene films used as control. The reduced mechanical properties of AOB film after UV treatment (340 nm) suggested that the AOB film could be degraded by oxo biodegradation. The developed AOB films can effectively prevent decomposition of food by providing antibacterial function and preserving freshness.

Preparation and Properties of Antimicrobial Zinc Alginate Films according to Solution Concentration (용액 농도에 따른 항균성 알긴산 아연 필름의 제조 및 그 특성)

  • Lee, Ju-Hyun;Seo, Hye-Jin;Son, Tae-Won;Lim, Hak-Sang
    • Polymer(Korea)
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    • v.37 no.6
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    • pp.677-684
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    • 2013
  • Zinc alginate films were prepared by a film maker from sodium alginate solutions of different weight ratios and then they solidified into 3 wt% content $ZnCl_2$ coagulation solution and washed and dried at a $60^{\circ}C$ oven for 20 min. The characteristics were measured by several methods (antimicrobial activity, viscosity, FTIR, TGA, SEM, EDS, contact angle, tensile strength and solubility) and the film properties were investigated. The antimicrobial test showed that the zinc alginate films result in excellent antimicrobial activity in the two strains (Klebsiella pneumonia, Staphylococcus). The surface of zinc alginate film from the solution of 9 wt% sodium alginate showed more uniform shape than any other films and the cross-section were hard and rough when the films were well-solidified by the $ZnCl_2$ solution. The tensile strength of zinc alginate films increased along with the concentration of sodium alginate solution due to the cross-linking, and the initial thermal decomposition temperature increased gradually.

Environment Corresponding Package by Quantitative Mixing System with Functional Inorganic Material and Polyolefin Resin (기능성 무기물과 폴리올레핀계 수지의 정량적 혼합시스템에 의한 환경대응형 포장소재 개발)

  • Kim, Hi-Sam;Lim, Hyun-Ju;Park, Young-Mi
    • Textile Coloration and Finishing
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    • v.21 no.1
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    • pp.1-9
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    • 2009
  • A lot of research has been made over the recent decade to develop testing packages with antimicrobial properties to improve food safety. In this study, a new method, experimental device and technology for environmental corresponding packages of polypropylene (PP) film has been developed to provide effective temperature buffering during the transport/long-term storage of grains or foodstuffs from the supplier to the market. This quantitatively optimized mixing system enabled to produce PP films with the 700$\sim$1,400d (width;1.5$\sim$3mm, thickness;0.01$\sim$0.5mm). In the whole mixing systems, the finely-granulated inorganic illite and PP virgin chip for master batch (M/B) chip was calculated by digital measurement methods, and then the M/B chip for PP film was adapted through a air jet and PP grinding method. The prepared PP film was characterized with tensile strength and elongation, far infrared radiation (FIR) emissivity, antimicrobial activity and deodorization properties. The results revealed that the two differently grain-sized illite could be show homogeneously dispersed on PP chip surface, and as the increasing of illite content, the FIR emissivity and the anion emission rate of film was increasingly improved. In both of 325 and 1,500 mesh-sized illite contained PP chip, of course the antimicrobial activity was good. But the ultimate deodorization rate for ammonia gas of PP film were found to be approximately the same.

A Study on the Antibacterial Properties of CPVC(Chlorinated polyvinyl chloride) Film treated with ODDMAC(Octadecyldimethyl (3-triethoxy silylpropyl) ammonium chloride) (CPVC(Chlorinated polyvinyl chloride)와 ODDMAC(Octadecyldimethyl(3-triethoxy silylpropyl) ammonium chloride) 첨가한 필름의 항균 특성)

  • Kim, Jiyeon;Lee, Sang Oh;Lee, Jaewoong
    • Textile Coloration and Finishing
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    • v.33 no.2
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    • pp.72-78
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    • 2021
  • The purpose of this study, the purpose of this study is to activate the antibacterial effect on the Chlorinated polyvinyl chloride film by using Octadecyldimethyl (3-triethoxy silylpropyl) ammonium chloride antibacterial agent with Chlorinated polyvinyl chloride polymer, which is inexpensive and has excellent properties such as heat resistance and chemical resistance. The Chlorinated polyvinyl chloride polymer was dissolved in a dimethylacetamide solvent, and film samples were prepared by varying the ratio of Octadecyldimethyl (3-triethoxy silylpropyl) ammonium chloride to study the antibacterial performance. A Scanning Electron Microscope-Energy Dispersive X-ray Spectrometer and X-ray photoelectron spectroscopy were employed to confirm the elements in the samples. According to the initial decomposition temperature of the Chlorinated polyvinyl chloride film and the Chlorinated polyvinyl chloride/Octadecyldimet hyl (3-triethoxy silylpropyl) ammonium chloride(10%) film using a Thermogravimetric analyzer(TA-DTA), it was confirmed that the initial decomposition temperature was lowered due to the influence of Octadecyldimethyl (3-triethoxy silylpropyl) ammonium chloride. In addition, in order to measure the mechanical properties, Universal testing machine was used and the result showed that a strength of Chlorinated polyvinyl chloride/Octadecyldimethyl (3-triethoxy silylpropyl) ammonium chloride(10%) was 36.8 MPa. The antimicrobial properties of the Chlorinated polyvinyl chloride/Octadecyldimethyl (3-triethoxy silylpropyl) ammonium chloride(10%) film showed 99.9% antimicrobial properties.

The Antimicrobial Food Packaging: Application of Antimicrobial Agents in Food Packaging (항균 식품포장: 식품 포장에서의 항균물질의 응용)

  • Cha, Dong-Su;Kweon, Dong-Keon;Park, Hyun-Jin
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.11 no.2
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    • pp.101-107
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    • 2005
  • The term 'antimicrobial' packaging encompasses any packaging technique(s) used to control microbial growth in the food product. These include packaging materials and edible films and coatings that contain antimicrobial agents, and also techniques that modify the atmosphere within the package. In recent years, antimicrobial packaging has attracted much attention from the food industry because of the increase in consumer demand for minimally processed, preservative-free products. Reflecting this demand, the preservative agents must be applied to packaging in such a way that only low levels of preservatives come into contact with the food. The film or coating technique is considered to be more effective, although more complicated to apply. New antimicrobial packaging materials are being developed continually. Many of them exploit natural agents, to control common food-borne microorganisms. Current trends suggest that in due course, packaging will generally incorporate antimicrobial agents and the sealing systems will continue to improve. The focus of packaging in the past has been on the appearance, size and integrity of the package. A greater emphasis on safety features associated with the addition of antimicrobial agents is perhaps the next area for development in packaging technology.

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Effect of Antimicrobial Dipping and Packaging on the Keeping Quality of Cucumbers (식물성 항균소재를 이용한 침지 및 포장처리가 오이의 선도에 미치는 영향)

  • 정순경;조성환
    • Food Science and Preservation
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    • v.7 no.1
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    • pp.8-11
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    • 2000
  • The antimicrobial extracts of rheum palmatum and coptis chinensis root as well as grapefruit seed extract(GFSE) were applied to dipping treatment for keeping qualities of cucumbers, respectively, which were then packed in low density polyethylene(LDPE) films incorporated with 1 % antimicrobial extracts and stored $10^{\circ}C$. Dipping and packaging in the antimicrobial agents suppressed the growth of putrefactive microorgani는 and the decay ratio of cucumbers. In addition, the loss ratio of ascortic acid content and their weight was decreased during the sotrage of cucumbers. Consequently, the combined method of dippinf and packaging in antimicrobial agents turned out to be superior to dipping treatment or film-packaging in the view point of decay ratio and the keeping qualities of cucumbers.

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Effect of Organic Photosensitizers on the Antimicrobial Property of Polyurethane coated Leather

  • Oh, Kyung Wha;Lim, Ki Sub
    • Fashion & Textile Research Journal
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    • v.15 no.4
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    • pp.630-634
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    • 2013
  • Cow leather coated with polyurethane film that contains various organic photosensitizers was investigated to demonstrate the antimicrobial properties in the application of the material to protective clothing and home appliances. To prepare the antimicrobial coating on leather surfaces with high potency against microbes, photoactive agents, such as benzophenone (BP), 4,4'-bis(dimethylamino) benzophenone (MK), 4,4'-dihydroxybenzophenone (DHBP) and methylene blue (MB), were incorporated into polyurethane-based coating solutions. The photoactive antimicrobial agent treated leather samples were characterized by SEM, color appearance, color fastness against abrasion, and antimicrobial tests. The optical properties of organic photosensitizers indicated that active UV absorbance ranges were different: BP (around 250 nm), MK (around 360 nm), DHBP (around 305 nm) and MB (around 295 nm &570 nm-685 nm). The intensity of the UV absorbance curve at the UVA light wavelength for the antimicrobial test showed the highest value with MK; subsequently, this was followed by MB, DHBP and BP in decreasing order. The treated-leather samples demonstrated excellent antibacterial activity under UVA light. The antimicrobial effects for the Staphylococcus aureus were superior to Escherichia coli. Moreover, the polyurethane finishing showed an effective durability to abrasion. The overall results indicated that DHBP is the most suitable PU coating additive to provide antimicrobial properties to leather as well as color and surface appearance than MK, MB, and BP.

Preparation of Gelatin Film Containing Grapefruit Seed Extract and Its Antimicrobial Effect (자몽종자 추출물을 함유한 Gelatin Film의 항균 효과)

  • Lim, Geum-Ok;Hong, Youn-Hee;Song, Kyung-Bin
    • Food Science and Preservation
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    • v.16 no.1
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    • pp.134-137
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    • 2009
  • The gelatin film containing grapefruit seed extract (GSE) was prepared by incorporating different amounts (0, 0.02, 0.05, 0.08, 0.1%) of GSE into the film. The tensile strength (TS) of the film increased by the addition of GSE, and water vapor permeability (WVP) of the film decreased. In particular, the gelatin film containing 0.1% GSE had a TS of 10.28 MPa, while the control had 8.68 MPa. WVP of the film containing 0.1% GSE decreased to 2.18 ng m/m2 s Pa, compared to 2.48 ng $m/m^{2}s$ Pa of the control. In addition, incorporation of 0.1% GSE to the gelatin film decreased the populations of Escherichia coli O157:H7 and Listeria monocytogenes by 2.67 and 3.15 log CFU/g, respectively, compared to the control. These results suggest that as a packaging material, gelatin film containing GSE can have antimicrobial activity against pathogenic microorganisms in foods.

Fabrication of Polyethylene Films Coated with Antimicrobials in a Binder and Their Application to Modified Atmosphere Packaging of Strawberries (결착제 함유 항균성 물질로 코팅한 폴리에틸렌 필름의 제조 및 이를 이용한 딸기의 환경기체조절포장)

  • 김영민;이상백;조성환;이동선
    • Food Science and Preservation
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    • v.7 no.1
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    • pp.12-18
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    • 2000
  • As am economical and effective way of antimicrobial film fabrication , antimicrobial agents were coated on the LDPE film with a binder mediu. the fabricated films were then applied tomodified atmosphere packaging of fresh strawberries. A binder of polyamide was selected for the coating medium, based on the stability in water. 1% grapefruit seed extract-coated film showed the antimicrobial activity on the plate media against EScherichia coli, Staphylococcus aureus, bacillus subtilis , Bacillus cereus, Leuconostoc mesenteroides, Micrococcus flavus, Saccharomyces cerevisiae, while one with 10% Coptis chinesis extract inhibited only M. 림편 and one coated with 10% rheum palmatum extract did not inhibit any of 10 strains tested. The packages of fresh strawberries by using antimicrobial agents-coated films created the gas compositions of O2 1.4-5.5% and CO2 5.7-7.9%, and contributed to reduced growth of total aerobic bacteria and yeast/molds on the produced. However, their lower microbial count was not correlated directly with the reduced decay of the fruits.

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