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http://dx.doi.org/10.4014/jmb.1512.12037

Chitosan-Based Film of Tyrothricin for Enhanced Antimicrobial Activity against Common Skin Pathogens Including Staphylococcus aureus  

Han, Sang Duk (Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories)
Sung, Hyun Jung (Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories)
Lee, Ga Hyeon (Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories)
Jun, Joon-Ho (Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories)
Son, Miwon (Dong-A ST Research Institute, Pharmaceutical Product Research Laboratories)
Kang, Myung Joo (College of Pharmacy, Dankook University)
Publication Information
Journal of Microbiology and Biotechnology / v.26, no.5, 2016 , pp. 953-958 More about this Journal
Abstract
Chitosan-based film-forming gel is regarded as a promising vehicle for topical delivery of antimicrobial agents to skin wounds, since it protects from microbial infection and the cationic polymer itself possesses antibacterial activity. In this study, possible synergistic interaction against common skin pathogens between the cationic polymer and tyrothricin (TRC), a cyclic polypeptide antibiotic, was investigated, by determining the concentration to inhibit 90% of bacterial isolates (MIC). The addition of the polysaccharide to TRC dramatically reduced the MIC values of TRC by 1/33 and 1/4 against both methicillin-resistant and methicillin-susceptible Staphylococcus aureus, respectively. The synergism of TRC and chitosan combination against both strains was demonstrated by the checkerboard method, with a fractional inhibitory concentration index below 0.5. Moreover, co-treatment of TRC and chitosan exhibited antibacterial activity against Pseudomonas aeruginosa, due to the antibacterial activity of chitosan, whereas TRC itself did not inhibit the gram-negative bacterial growth. These findings suggested that the use of chitosan-based film for topical delivery of TRC could be an alternative to improve TRC antimicrobial activity against strains that are abundant in skin wounds.
Keywords
Tyrothricin; chitosan; antimicrobial activity; Staphylococcus aureus; skin infection;
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Times Cited By KSCI : 1  (Citation Analysis)
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